P22815 (BOSS_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein bride of sevenless

Gene names

Name:	boss
ORF Names:	CG8285

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	896 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as a ligand for sevenless tyrosine-kinase receptor during eye development. Ref.5
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Tissue specificity	Expressed exclusively by R8 photoreceptor cells and is internalized in a sev-dependent manner by R7 cells.
Sequence similarities	Belongs to the G-protein coupled receptor 3 family.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	R7 cell development Non-traceable author statement. Source: FlyBase R7 cell fate commitment Traceable author statement. Source: FlyBase R8 cell-mediated photoreceptor organization Non-traceable author statement. Source: FlyBase glucose homeostasis Inferred from mutant phenotype. Source: FlyBase lipid homeostasis Inferred from mutant phenotype. Source: FlyBase response to glucose stimulus Inferred from mutant phenotype. Source: FlyBase visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Non-traceable author statement. Source: FlyBase microvillus Inferred from direct assay Ref.5. Source: FlyBase plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW sevenless binding Inferred from genetic interaction Ref.5. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 896	865	Protein bride of sevenless		PRO_0000012971
Regions
	Topological domain	32 – 530	499	Extracellular Potential
	Transmembrane	531 – 554	24	Helical; Potential
	Transmembrane	570 – 588	19	Helical; Potential
	Transmembrane	615 – 637	23	Helical; Potential
	Transmembrane	655 – 676	22	Helical; Potential
	Transmembrane	693 – 712	20	Helical; Potential
	Transmembrane	728 – 748	21	Helical; Potential
	Transmembrane	759 – 781	23	Helical; Potential
	Topological domain	782 – 896	115	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	183	1	N-linked (GlcNAc...) Potential
	Glycosylation	307	1	N-linked (GlcNAc...) Potential
	Glycosylation	474	1	N-linked (GlcNAc...) Potential
	Glycosylation	485	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P22815 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: 580AEBC0FA208E24	FASTA	896	99,950
	10 20 30 40 50 60 MKVMDALQSG RRKPLPVALL CILVTVFCVL ECHGADLTSP TKKSAPLRIT KPQPTSQQAK 70 80 90 100 110 120 PISITTRAPT TVASTTDDEV SSSVDGQLAP LISSTTEGPS SGTTASLVPE ICLNGLQLTV 130 140 150 160 170 180 NSADEGTVIR KQEEFVKILE GDVVLSVLTK DPDSALFVIN RVNQANLIMA DFEIGIRAIS 190 200 210 220 230 240 IDNASLAENL LIQEVQFLQQ CTTYSMGIFV DWELYKQLES VIKDLEYNIW PIPGTRAHLF 250 260 270 280 290 300 PKVAHLLHQM PWGEKIASVE IATETLEMYN EFMEAARQEH MCLMHFKSDD NVYIMFGNKL 310 320 330 340 350 360 ASHFKENGTL FSVPTDRTDD EFLADLPNRA FVLMENEIDL STAVELDATP TALDEILIGK 370 380 390 400 410 420 SVLPSRVLSF AGSIIDLMNW LRGSLSKHCK RGEEHDLYVL ESCFNFLNFI EDWRTSEYRQ 430 440 450 460 470 480 AHDTAEILSL LLMRKLGTAM NFQMYQKKVL TLDKITGESR TELREIASQN FVTNVTTYYH 490 500 510 520 530 540 YNRDNHTSLE LKTKFGQVFN CQYSAGDNRR YPFLFDGESV MFWRIKMDTW VATGLTAAIL 550 560 570 580 590 600 GLIATLAILV FIVVRISLGD VFEGNPTTSI LLLLSLILVF CSFVPYSIEY VGEQRNSHVT 610 620 630 640 650 660 FEDAQTLNTL CAVRVFIMTL VYCFVFSLLL CRAVMLASIG SEGGFLSHVN GYIQAVICAF 670 680 690 700 710 720 SVVAQVGMSV QLLVVMHVAS ETVSCENIYY GRWLWGLLAY DFALLCCVGA LIPSIYRSQR 730 740 750 760 770 780 NYREGILIVI GSVLIMVIWV AWIALSLFGD EWRDAAIPLG LQASGWAVLV GILIPRTFLI 790 800 810 820 830 840 VRGIERSDIA QALPSLTSLA FAQNNQYSSE QSVYECVNPA MRHCSQDEVN HQSPSEIPTL 850 860 870 880 890 PLRGGGPRRQ QFFANLRQAN ANINPQRPPP RPQQSPSRSS VSSLPPSPDH NKITRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Induction of cell fate in the Drosophila retina: the bride of sevenless protein is predicted to contain a large extracellular domain and seven transmembrane segments." Hart A.C., Kraemer H., van Vactor D.L. Jr., Paidhungat M., Zipursky S.L. Genes Dev. 4:1835-1847(1990) [PubMed: 2276620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Kraemer H. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"Interaction of bride of sevenless membrane-bound ligand and the sevenless tyrosine-kinase receptor." Kraemer H., Cagan R.L., Zipursky S.L. Nature 352:207-212(1991) [PubMed: 1857416] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55887 Genomic DNA. Translation: CAA39373.1. AE014297 Genomic DNA. Translation: AAF56539.1.
RefSeq	NP_542440.1. NM_080709.3.
UniGene	Dm.2358.
3D structure databases
ProteinModelPortal	P22815.
ModBase	Search...
Protein-protein interaction databases
STRING	P22815.
Protein family/group databases
GPCRDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0084949; FBpp0084323; FBgn0000206.
GeneID	43146.
KEGG	dme:Dmel_CG8285.
NMPDR	fig|7227.3.peg.14754.
Organism-specific databases
CTD	43146.
FlyBase	FBgn0000206. boss.
Phylogenomic databases
eggNOG	inNOG08637.
InParanoid	P22815.
OMA	ECVNPAM.
OrthoDB	EOG49CNPM.
PhylomeDB	P22815.
Gene expression databases
Bgee	P22815.
GermOnline	CG8285. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002956. Bride_of_7less. IPR017978. GPCR_3_C. [Graphical view]
KO	K04623.
Pfam	PF00003. 7tm_3. 1 hit. [Graphical view]
PRINTS	PR01223. BRIDEOF7LESS.
PROSITE	PS00979. G_PROTEIN_RECEP_F3_1. False negative. PS00980. G_PROTEIN_RECEP_F3_2. False negative. PS00981. G_PROTEIN_RECEP_F3_3. False negative. PS50259. G_PROTEIN_RECEP_F3_4. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	832403.

Entry information

Entry name

BOSS_DROME

Accession

Primary (citable) accession number: P22815
Secondary accession number(s): Q9VBJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project