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Protein

Heat shock factor protein

Gene

Hsf

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi46 – 150Add BLAST105

GO - Molecular functioni

  • DNA binding Source: FlyBase
  • DNA binding transcription factor activity Source: FlyBase
  • identical protein binding Source: IntAct
  • sequence-specific DNA binding Source: FlyBase

GO - Biological processi

  • defense response to bacterium Source: FlyBase
  • defense response to fungus Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: FlyBase
  • positive regulation of DNA binding Source: FlyBase
  • positive regulation of transcription, DNA-templated Source: FlyBase
  • positive regulation of transcription by RNA polymerase II Source: FlyBase
  • response to heat Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processStress response, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-3371453 Regulation of HSF1-mediated heat shock response
R-DME-3371511 HSF1 activation
R-DME-3371568 Attenuation phase
R-DME-3371571 HSF1-dependent transactivation

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock factor protein
Short name:
HSF
Alternative name(s):
Heat shock transcription factor
Short name:
HSTF
Gene namesi
Name:Hsf
ORF Names:CG5748
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0001222 Hsf

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001245781 – 691Heat shock factor proteinAdd BLAST691

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei256Phosphoserine1 Publication1
Modified residuei258Phosphothreonine1 Publication1
Modified residuei260Phosphoserine1 Publication1
Modified residuei283Phosphoserine1 Publication1
Modified residuei299Phosphoserine1 Publication1
Modified residuei580Phosphoserine1 Publication1

Post-translational modificationi

Exhibits temperature-dependent phosphorylation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP22813

PTM databases

iPTMnetiP22813

Expressioni

Gene expression databases

BgeeiFBgn0001222
ExpressionAtlasiP22813 differential
GenevisibleiP22813 DM

Interactioni

Subunit structurei

Homotrimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-130048,EBI-130048

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi62751, 17 interactors
DIPiDIP-20041N
IntActiP22813, 9 interactors
MINTiP22813

Structurei

Secondary structure

1691
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi50 – 57Combined sources8
Beta strandi58 – 60Combined sources3
Turni61 – 65Combined sources5
Beta strandi66 – 69Combined sources4
Turni70 – 72Combined sources3
Beta strandi74 – 76Combined sources3
Turni82 – 87Combined sources6
Turni89 – 92Combined sources4
Helixi97 – 107Combined sources11
Beta strandi115 – 117Combined sources3
Beta strandi128 – 130Combined sources3
Turni142 – 144Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HKSNMR-A43-148[»]
1HKTNMR-A43-148[»]
ProteinModelPortaliP22813
SMRiP22813
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22813

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi16 – 26Poly-GluAdd BLAST11

Sequence similaritiesi

Belongs to the HSF family.Curated

Phylogenomic databases

InParanoidiP22813
KOiK09414
OMAiMQLMIND
OrthoDBiEOG091G087O

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000232 HSF_DNA-bd
IPR027725 HSF_fam
IPR010542 Vert_HSTF_C
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10015 PTHR10015, 2 hits
PfamiView protein in Pfam
PF00447 HSF_DNA-bind, 1 hit
PF06546 Vert_HS_TF, 1 hit
PRINTSiPR00056 HSFDOMAIN
SMARTiView protein in SMART
SM00415 HSF, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00434 HSF_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

P22813-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRSRSSAKA VQFKHESEEE EEDEEEQLPS RRMHSYGDAA AIGSGVPAFL
60 70 80 90 100
AKLWRLVDDA DTNRLICWTK DGQSFVIQNQ AQFAKELLPL NYKHNNMASF
110 120 130 140 150
IRQLNMYGFH KITSIDNGGL RFDRDEIEFS HPFFKRNSPF LLDQIKRKIS
160 170 180 190 200
NNKNGDDKGV LKPEAMSKIL TDVKVMRGRQ DNLDSRFSAM KQENEVLWRE
210 220 230 240 250
IASLRQKHAK QQQIVNKLIQ FLITIVQPSR NMSGVKRHVQ LMINNTPEID
260 270 280 290 300
RARTTSETES ESGGGPVIHE LREELLDEVM NPSPAGYTAA SHYDQESVSP
310 320 330 340 350
PAVERPRSNM SISSHNVDYS NQSVEDLLLQ GNGTAGGNIL VGGAASPMAQ
360 370 380 390 400
SVSQSPAQHD VYTVTEAPDS HVQEVPNSPP YYEEQNVLTT PMVREQEQQK
410 420 430 440 450
RQQLKENNKL RRQAGDVILD AGDILVDSSS PKAQRTSIQH STQPDVMVQP
460 470 480 490 500
MIIKSEPENS SGLMDLMTPA NDLYSVNFIS EDMPTDIFED ALLPDGVEEA
510 520 530 540 550
AKLDQQQKFG QSTVSSGKFA SNFDVPTNST LLDANQASTS KAAAKAQASE
560 570 580 590 600
EEGMAVAKYS GAENGNNRDT NNSQLLRMAS VDELHGHLES MQDELETLKD
610 620 630 640 650
LLRGDGVAID QNMLMGLFND SDLMDNYGLS FPNDSISSEK KAPSGSELIS
660 670 680 690
YQPMYDLSDI LDTDDGNNDQ EASRRQMQTQ SSVLNTPRHE L
Length:691
Mass (Da):76,933
Last modified:August 1, 1991 - v1
Checksum:iE013428B22C98D35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60070 mRNA Translation: AAA28642.1
AE013599 Genomic DNA Translation: AAF57749.1
PIRiA36295
RefSeqiNP_476575.1, NM_057227.5
UniGeneiDm.4107

Genome annotation databases

EnsemblMetazoaiFBtr0086782; FBpp0085961; FBgn0001222
GeneIDi37068
KEGGidme:Dmel_CG5748

Similar proteinsi

Entry informationi

Entry nameiHSF_DROME
AccessioniPrimary (citable) accession number: P22813
Secondary accession number(s): Q9V8C1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: March 28, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health