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Protein

Protein Tube

Gene

tub

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in the Tl receptor signaling pathway that establishes embryonic dorsoventral polarity; the signal directs import of dl into ventral and ventrolateral nuclei, thereby establishing dorsoventral polarity. Tub recruits pll to the plasma membrane and protein-protein interaction activates pll. Also has a role in pupal pattern formation.5 Publications

GO - Molecular functioni

GO - Biological processi

  • antifungal humoral response Source: FlyBase
  • apoptotic process Source: UniProtKB
  • defense response Source: FlyBase
  • determination of dorsal/ventral asymmetry Source: UniProtKB
  • dorsal/ventral axis specification Source: FlyBase
  • hemocyte proliferation Source: FlyBase
  • hemopoiesis Source: FlyBase
  • innate immune response Source: UniProtKB
  • intracellular signal transduction Source: GO_Central
  • larval somatic muscle development Source: FlyBase
  • response to fungus Source: FlyBase
  • Toll signaling pathway Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, Transducer

Enzyme and pathway databases

ReactomeiR-DME-209442. Formation of the trans-membrane 'signalling complex'.
R-DME-214842. DL and DIF homodimers bind to TUB and phosphorylated PLL in the TL receptor 'signalling complex'.
R-DME-214844. DL and DIF homodimers complexed with CACT are all phosphorylated in the TL receptor 'signalling complex'.
R-DME-214862. Activated PLL kinase is autophosphorylated in the TL receptor 'signalling complex'.
R-DME-214863. Adaptor protein complex binds to TL receptor at the plasma membrane.
R-DME-214869. Phosphorylated CACT, DL and DIF homodimers dissociate from the TL receptor 'signalling complex'.
R-DME-214874. PLL kinase binds to TUB in the TL receptor 'signalling complex'.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Tube
Gene namesi
Name:tub
Synonyms:tube
ORF Names:CG10520
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003882. tub.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000656941 – 462Protein TubeAdd BLAST462

Post-translational modificationi

Phosphorylated by pll.1 Publication

Proteomic databases

PaxDbiP22812.
PRIDEiP22812.

Expressioni

Developmental stagei

Expressed both maternally and zygotically. Zygotic expression is highest in late larval development.1 Publication

Gene expression databases

BgeeiFBgn0003882.
GenevisibleiP22812. DM.

Interactioni

Subunit structurei

Interacts (via Death domain) with pll (via Death domain).4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein domain specific binding Source: UniProtKB

Protein-protein interaction databases

BioGridi65774. 12 interactors.
DIPiDIP-27623N.
IntActiP22812. 7 interactors.
MINTiMINT-247876.
STRINGi7227.FBpp0271525.

Structurei

Secondary structure

1462
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi33 – 35Combined sources3
Helixi38 – 49Combined sources12
Helixi50 – 52Combined sources3
Helixi53 – 60Combined sources8
Beta strandi62 – 64Combined sources3
Helixi67 – 71Combined sources5
Helixi78 – 81Combined sources4
Helixi82 – 84Combined sources3
Beta strandi86 – 88Combined sources3
Helixi90 – 102Combined sources13
Helixi109 – 118Combined sources10
Beta strandi121 – 123Combined sources3
Helixi129 – 138Combined sources10
Helixi142 – 151Combined sources10
Beta strandi162 – 164Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D2ZX-ray2.00B/D23-175[»]
ProteinModelPortaliP22812.
SMRiP22812.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22812.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 152DeathAdd BLAST126
Repeati262 – 26918
Repeati286 – 29328
Repeati319 – 32638
Repeati356 – 36348
Repeati453 – 46058

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni262 – 4605 X approximate repeatsAdd BLAST199

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi388 – 401Asp/Glu-rich (acidic)Add BLAST14

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410J7N2. Eukaryota.
ENOG410Z3IC. LUCA.
InParanoidiP22812.
KOiK04733.
OMAiNDDYACN.
OrthoDBiEOG091G0Q4E.
PhylomeDBiP22812.

Family and domain databases

InterProiView protein in InterPro
IPR011029. DEATH-like_dom_sf.
IPR000488. Death_domain.
IPR029397. Tube_Death.
PfamiView protein in Pfam
PF14786. Death_2. 1 hit.
SMARTiView protein in SMART
SM00005. DEATH. 1 hit.
SUPFAMiSSF47986. SSF47986. 1 hit.

Sequencei

Sequence statusi: Complete.

P22812-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYGWNGCGM GVQVNGSNGA IGLSSKYSRN TELRRVEDND IYRLAKILDE
60 70 80 90 100
NSCWRKLMSI IPKGMDVQAC SGAGCLNFPA EIKKGFKYTA QDVFQIDEAA
110 120 130 140 150
NRLPPDQSKS QMMIDEWKTS GKLNERPTVG VLLQLLVQAE LFSAADFVAL
160 170 180 190 200
DFLNESTPAR PVDGPGALIS LELLEEEMEV DNEGLSLKYQ SSTATLGADA
210 220 230 240 250
QGSVGLNLDN FEKDIVRRDK SVPQPSGNTP PIAPPRRQQR STTNSNFATL
260 270 280 290 300
TGTGTTSTTI PNVPNLTILN PSEQIQEPVL QPRPMNIPDL SILISNSGDL
310 320 330 340 350
RATVSDNPSN RTSSTDPPNI PRITLLIDNS GDVNSRPNHA PAKASTATTS
360 370 380 390 400
TASSNNLPMI SALNISKGSR ETLRPESRSS SSSLSKDDDD DNDGEEDGEE
410 420 430 440 450
EYPDAFLPNL SNSEQQSSNN DSSLTTVTGT SGDNSFELTN DSSSTSNDDY
460
ACNIPDLSEL QQ
Length:462
Mass (Da):49,776
Last modified:April 14, 2009 - v4
Checksum:iDDFBB9D72B8036FC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti350S → P in AAA28994 (PubMed:1899484).Curated1
Sequence conflicti370R → K in AAA28994 (PubMed:1899484).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59501 mRNA. Translation: AAA28994.1.
AE014297 Genomic DNA. Translation: ADV37257.1.
AY061215 mRNA. Translation: AAL28763.1.
BT125884 mRNA. Translation: ADV15463.1.
PIRiA37862. A33170.
RefSeqiNP_001189164.1. NM_001202235.2.
UniGeneiDm.2494.

Genome annotation databases

EnsemblMetazoaiFBtr0302338; FBpp0291542; FBgn0003882.
GeneIDi40554.
KEGGidme:Dmel_CG10520.
UCSCiCG10520-RB. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiTUBE_DROME
AccessioniPrimary (citable) accession number: P22812
Secondary accession number(s): B7Z0I9, E8NH30, Q9VN15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: April 14, 2009
Last modified: November 22, 2017
This is version 165 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references