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P22811

- XDH_DROPS

UniProt

P22811 - XDH_DROPS

Protein

Xanthine dehydrogenase

Gene

ry

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (11 Jul 2006)
      Previous versions | rss
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    Functioni

    Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid By similarity.By similarity

    Catalytic activityi

    Xanthine + NAD+ + H2O = urate + NADH.
    Hypoxanthine + NAD+ + H2O = xanthine + NADH.

    Cofactori

    Binds 2 2Fe-2S clusters.By similarity
    FAD.By similarity
    Binds 1 molybdenum-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi47 – 471Iron-sulfur 1By similarity
    Metal bindingi52 – 521Iron-sulfur 1By similarity
    Metal bindingi55 – 551Iron-sulfur 1By similarity
    Metal bindingi77 – 771Iron-sulfur 1By similarity
    Metal bindingi117 – 1171Iron-sulfur 2By similarity
    Metal bindingi120 – 1201Iron-sulfur 2By similarity
    Metal bindingi152 – 1521Iron-sulfur 2By similarity
    Metal bindingi154 – 1541Iron-sulfur 2By similarity
    Binding sitei343 – 3431FADBy similarity
    Binding sitei366 – 3661FADBy similarity
    Binding sitei414 – 4141FAD; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei432 – 4321FADBy similarity
    Metal bindingi780 – 7801MolybdenumBy similarity
    Metal bindingi811 – 8111Molybdenum; via carbonyl oxygenBy similarity
    Binding sitei815 – 8151SubstrateBy similarity
    Binding sitei893 – 8931SubstrateBy similarity
    Metal bindingi925 – 9251Molybdenum; via amide nitrogenBy similarity
    Binding sitei927 – 9271SubstrateBy similarity
    Metal bindingi1092 – 10921Molybdenum; via amide nitrogenBy similarity
    Active sitei1275 – 12751Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi263 – 2708FADBy similarity
    Nucleotide bindingi353 – 3575FADBy similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB
    2. electron carrier activity Source: InterPro
    3. flavin adenine dinucleotide binding Source: UniProtKB
    4. iron ion binding Source: InterPro
    5. molybdopterin cofactor binding Source: UniProtKB
    6. UDP-N-acetylmuramate dehydrogenase activity Source: InterPro
    7. xanthine dehydrogenase activity Source: UniProtKB
    8. xanthine oxidase activity Source: InterPro

    GO - Biological processi

    1. xanthine catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xanthine dehydrogenase (EC:1.17.1.4)
    Short name:
    XD
    Alternative name(s):
    Protein rosy locus
    Gene namesi
    Name:ry
    Synonyms:XDH
    ORF Names:GA20500
    OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
    Taxonomic identifieri46245 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000001819: Chromosome 2, UP000001819: Partially assembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0012736. Dpse\ry.

    Subcellular locationi

    Peroxisome By similarity

    GO - Cellular componenti

    1. peroxisome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13431343Xanthine dehydrogenasePRO_0000166080Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi7237.FBpp0282224.

    Structurei

    3D structure databases

    ProteinModelPortaliP22811.
    SMRiP22811. Positions 9-169.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 95882Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini235 – 424190FAD-binding PCMH-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the xanthine dehydrogenase family.Curated
    Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
    Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG4630.
    InParanoidiP22811.
    KOiK00106.
    OrthoDBiEOG7QRQSZ.

    Family and domain databases

    Gene3Di1.10.150.120. 1 hit.
    3.10.20.30. 1 hit.
    3.30.365.10. 6 hits.
    3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    3.90.1170.50. 1 hit.
    InterProiIPR002888. 2Fe-2S-bd.
    IPR001041. 2Fe-2S_ferredoxin-type.
    IPR006058. 2Fe2S_fd_BS.
    IPR000674. Ald_Oxase/Xan_DH_a/b.
    IPR016208. Ald_Oxase/xanthine_DH.
    IPR008274. AldOxase/xan_DH_Mopterin-bd.
    IPR012675. Beta-grasp_dom.
    IPR005107. CO_DH_flav_C.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR002346. Mopterin_DH_FAD-bd.
    IPR022407. OxRdtase_Mopterin_BS.
    IPR014307. Xanthine_DH_ssu.
    [Graphical view]
    PfamiPF01315. Ald_Xan_dh_C. 1 hit.
    PF02738. Ald_Xan_dh_C2. 1 hit.
    PF03450. CO_deh_flav_C. 1 hit.
    PF00941. FAD_binding_5. 1 hit.
    PF00111. Fer2. 1 hit.
    PF01799. Fer2_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000127. Xanthine_DH. 1 hit.
    SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
    SM01092. CO_deh_flav_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF47741. SSF47741. 1 hit.
    SSF54292. SSF54292. 1 hit.
    SSF54665. SSF54665. 1 hit.
    SSF55447. SSF55447. 1 hit.
    SSF56003. SSF56003. 1 hit.
    SSF56176. SSF56176. 1 hit.
    TIGRFAMsiTIGR02963. xanthine_xdhA. 1 hit.
    PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
    PS51085. 2FE2S_FER_2. 1 hit.
    PS51387. FAD_PCMH. 1 hit.
    PS00559. MOLYBDOPTERIN_EUK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P22811-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGQQKTSEL VFFVNGKKVT DTNPDPECTL LTYLRDKLRL CGTKLGCAEG     50
    GCGACTVVIS RMDRGQNKIR HLAVNACLTP VCAMHGCAVT TVEGIGSTRT 100
    RLHPVQERLA KAHGSQCGFC TPGIVMSMYA LLRSAEQPSM RDLEVAFQGN 150
    LCRCTGYRPI LEGYKTFTKE FACGMGDKCC KVNGKGCGGG DDTQSVTDDT 200
    LFERSQFQPL DPSQEPIFPP ELQLTPTYDS ESLIFSSERV TWYRPTTLQE 250
    LLQLKSDHPS AKLVVGNTEV GVEVKFKHFL YPHLINPTQV PELLEVRESE 300
    ESIYFGAAVS LMEIDALLRQ RIEELPEAQT RLFQCAVDML HYFAGKQIRN 350
    VACLGGNIMT GSPISDMNPV LTAAGARLEV ASLVGGKTSH RTVHMGTGFF 400
    TGYRRNVIEP HEVLLGIHFQ KTTPDQHVVA FKQARRRDDD IAIVNAAVNV 450
    RFEPRTNVVA GISMAFGGMA PTTVLAPRTS QLMVKQPLDH HLVERVAESL 500
    CGELPLAASA PGGMIAYRRA LVVSLIFKAY LSISRKLSEA GIISTDAIPA 550
    EERSGAELFH TPVLRSAQLF ERVCSEQPVC DPIGRPEVHA AALKQATGEA 600
    IYTDDIPRMD GELYLGLVLS TKPRAKITKL DASEALALEG VHAFFSHKDL 650
    TEHENEVGPV FHDEHVFAAA EVHCYGQIVG AVAADNKALA QRAARLVRVE 700
    YEELAPVIVT IEQAIEHGSY FPDYPRYVNK GNVEEAFAAA EHTYEGSCRM 750
    GGQEHFYLET HAAVAVPRDS DELELFCSTQ HPSEVQKLVA HVTTLPAHRV 800
    VCRAKRLGGG FGGKESRGIS VALPVALAAY RLRRPVRCML DRDEDMLITG 850
    TRHPFLFKYK VAFASDGLIT ACDIECYNNA GWSMDLSFSV LERAMYHFEN 900
    CYRIPNVRVG GWVCKTNLPS NTAFRGFGGP QGMFAGEHII RDVARIVGRD 950
    VLDVMRLNFY KTGDITHYNQ KLEHFPIERC LDDCLAQSRY HEKRTEIAKF 1000
    NRENRWRKRG MAVIPTKYGI AFGVMHLNQA GALINVYGDG SVLLSHGGVE 1050
    IGQGLNTKMI QCAARALGIP IELIHISETA TDKVPNTSPT AASVGSDLNG 1100
    MAVLDACEKL NKRLAPIKEA LPQGTWQEWI NKAYFDRVSL SATGFYAMPG 1150
    IGYHPETNPN ARTYSYYTNG VGISVVEIDC LTGDHQVLST DIVMDIGSSI 1200
    NPAIDIGQIE GAFMQGYGLF TLEELMYSPQ GMLYSRGPGM YKLPGFADIP 1250
    GEFNVSLLTG APNPRAVYSS KAVGEPPLFI GSSAFFAIKE AIAAARQEQG 1300
    LTGDFPLEAP STSARIRMAC QDKFTNLLEI PEEGSFTPWN IVP 1343
    Length:1,343
    Mass (Da):147,349
    Last modified:July 11, 2006 - v2
    Checksum:iA58C142336E14BC9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti58 – 581V → M in AAA29022. (PubMed:2493563)Curated
    Sequence conflicti200 – 2001T → A in AAA29022. (PubMed:2493563)Curated
    Sequence conflicti428 – 4281V → I in AAA29022. (PubMed:2493563)Curated
    Sequence conflicti428 – 4281V → I in AAX13180. (PubMed:15545653)Curated
    Sequence conflicti461 – 4611G → E in AAA29022. (PubMed:2493563)Curated
    Sequence conflicti461 – 4611G → E in AAX13180. (PubMed:15545653)Curated
    Sequence conflicti475 – 4751L → V in AAA29022. (PubMed:2493563)Curated
    Sequence conflicti734 – 7341E → D in AAX13180. (PubMed:15545653)Curated
    Sequence conflicti747 – 7471S → N in AAA29022. (PubMed:2493563)Curated
    Sequence conflicti762 – 7621A → G in AAA29022. (PubMed:2493563)Curated
    Sequence conflicti986 – 9861A → V in AAX13180. (PubMed:15545653)Curated
    Sequence conflicti1245 – 12451Missing in AAA29022. (PubMed:2493563)Curated
    Sequence conflicti1320 – 13201C → F in AAA29022. (PubMed:2493563)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33977 Genomic DNA. Translation: AAA29022.1.
    CM000070 Genomic DNA. Translation: EAL27642.2.
    AY754605 Genomic DNA. Translation: AAX13180.1.
    PIRiA31946.
    RefSeqiXP_001358503.2. XM_001358466.2.

    Genome annotation databases

    EnsemblMetazoaiFBtr0283786; FBpp0282224; FBgn0012736.
    GeneIDi4801405.
    KEGGidpo:Dpse_GA20500.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33977 Genomic DNA. Translation: AAA29022.1 .
    CM000070 Genomic DNA. Translation: EAL27642.2 .
    AY754605 Genomic DNA. Translation: AAX13180.1 .
    PIRi A31946.
    RefSeqi XP_001358503.2. XM_001358466.2.

    3D structure databases

    ProteinModelPortali P22811.
    SMRi P22811. Positions 9-169.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7237.FBpp0282224.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0283786 ; FBpp0282224 ; FBgn0012736 .
    GeneIDi 4801405.
    KEGGi dpo:Dpse_GA20500.

    Organism-specific databases

    FlyBasei FBgn0012736. Dpse\ry.

    Phylogenomic databases

    eggNOGi COG4630.
    InParanoidi P22811.
    KOi K00106.
    OrthoDBi EOG7QRQSZ.

    Family and domain databases

    Gene3Di 1.10.150.120. 1 hit.
    3.10.20.30. 1 hit.
    3.30.365.10. 6 hits.
    3.30.43.10. 1 hit.
    3.30.465.10. 1 hit.
    3.90.1170.50. 1 hit.
    InterProi IPR002888. 2Fe-2S-bd.
    IPR001041. 2Fe-2S_ferredoxin-type.
    IPR006058. 2Fe2S_fd_BS.
    IPR000674. Ald_Oxase/Xan_DH_a/b.
    IPR016208. Ald_Oxase/xanthine_DH.
    IPR008274. AldOxase/xan_DH_Mopterin-bd.
    IPR012675. Beta-grasp_dom.
    IPR005107. CO_DH_flav_C.
    IPR016169. CO_DH_flavot_FAD-bd_sub2.
    IPR016166. FAD-bd_2.
    IPR016167. FAD-bd_2_sub1.
    IPR002346. Mopterin_DH_FAD-bd.
    IPR022407. OxRdtase_Mopterin_BS.
    IPR014307. Xanthine_DH_ssu.
    [Graphical view ]
    Pfami PF01315. Ald_Xan_dh_C. 1 hit.
    PF02738. Ald_Xan_dh_C2. 1 hit.
    PF03450. CO_deh_flav_C. 1 hit.
    PF00941. FAD_binding_5. 1 hit.
    PF00111. Fer2. 1 hit.
    PF01799. Fer2_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000127. Xanthine_DH. 1 hit.
    SMARTi SM01008. Ald_Xan_dh_C. 1 hit.
    SM01092. CO_deh_flav_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47741. SSF47741. 1 hit.
    SSF54292. SSF54292. 1 hit.
    SSF54665. SSF54665. 1 hit.
    SSF55447. SSF55447. 1 hit.
    SSF56003. SSF56003. 1 hit.
    SSF56176. SSF56176. 1 hit.
    TIGRFAMsi TIGR02963. xanthine_xdhA. 1 hit.
    PROSITEi PS00197. 2FE2S_FER_1. 1 hit.
    PS51085. 2FE2S_FER_2. 1 hit.
    PS51387. FAD_PCMH. 1 hit.
    PS00559. MOLYBDOPTERIN_EUK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the Xdh region in Drosophila pseudoobscura and an analysis of the evolution of synonymous codons."
      Riley M.A.
      Mol. Biol. Evol. 6:33-52(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution."
      Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L.
      , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A.
      Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MV2-25 / Tucson 14011-0121.94.
    3. "Patterns of selection on synonymous and nonsynonymous variants in Drosophila miranda."
      Bartolome C., Maside X., Yi S., Grant A.L., Charlesworth B.
      Genetics 169:1495-1507(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 298-1062.

    Entry informationi

    Entry nameiXDH_DROPS
    AccessioniPrimary (citable) accession number: P22811
    Secondary accession number(s): Q299Q9, Q56RA2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: July 11, 2006
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3