Reviewed, UniProtKB/Swiss-Prot P22810 (HMOC_DROME)

Names and origin

Protein names

Recommended name:
    Homeotic protein ocelliless
Alternative name(s):
    Protein orthodenticle

Gene names

Name:	oc
Synonyms:	Otd
ORF Names:	CG12154

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	542 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transcriptional regulator involved in pattern formation and cell determination in the embryonic CNS and larval imaginal disks. Also later in development to coordinate the expression of regulatory and structural genes required for photoreceptor cell fate in the ocelli. Has a dual role in the terminal differentiation of subtypes of photoreceptors by regulating rhodopsin (rh) expression: essential for establishing the expression of rh genes in the pale subset of ommatidia as well as repressing Rh6 in outer photoreceptors. Ref.1 Ref.5 Ref.6
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in the anterior region of the embryo before cellularization and becomes localized to the procephalic head region following gastrulation. These cells correspond to the mesectoderm or midline neuroepithelium and will generate a mixed population of neurons and glia. Expressed in photoreceptors in pupae and adults. Ref.1 Ref.5 Ref.6
Developmental stage	Expressed from early embryonic stages through to late pupation. Ref.1
Domain	Contains multiple repeats consisting of single amino acids (e.g., Gly, Ser, His, and Asn) and pairs of amino acids (e.g., Gly-Val).
Disruption phenotype	In embryos, formation of an abnormal neuropil and the disappearance of the horizontal commissures associated with the midline of the CNS. In pupae, misshapen, duplicated, and shortened rhabdomeres within the eye. In adults, deletion of the ocelli. Ref.1 Ref.5
Sequence similarities	Belongs to the paired homeobox family. Contains 1 homeobox DNA-binding domain.
Sequence caution	The sequence CAA41732.1 differs from that shown. Reason: Frameshift at position 431.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox Repeat
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	adult walking behavior Traceable author statement. Source: FlyBase anterior head segmentation Traceable author statement. Source: FlyBase anterior region determination Traceable author statement. Source: FlyBase brain segmentation Inferred from mutant phenotype. Source: FlyBase embryonic development via the syncytial blastoderm Inferred from mutant phenotype. Source: FlyBase photoreceptor cell fate commitment Ref.5 Inferred from mutant phenotype. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW rhabdomere development Ref.5 Ref.6 Inferred from mutant phenotype. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 542	542	Homeotic protein ocelliless		PRO_0000049072
Regions
	DNA binding	67 – 126	60	Homeobox
	Compositional bias	132 – 268	137	Ser-rich
	Compositional bias	156 – 264	109	Asn-rich
	Compositional bias	210 – 294	85	Ala-rich
	Compositional bias	323 – 404	82	Gly-rich
Experimental info
	Sequence conflict	61	1	G → GDLCYPG in CAA41732. Ref.1
	Sequence conflict	240	1	A → ASAQSIKTHHSSFLSAAAA in CAA41732. Ref.1
	Sequence conflict	272 – 273	2	Missing in CAA41732. Ref.1
	Sequence conflict	337	1	G → GGG in CAA41732. Ref.1
	Sequence conflict	434	1	Missing in CAA41732. Ref.1
	Sequence conflict	530	1	D → Y in AAR88546. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P22810-1 [UniParc]. Last modified April 14, 2009. Version 2. Checksum: 73F6795CD8171EA0	FASTA	542	55,187
	10 20 30 40 50 60 MAAGFLKSGD LGPHPHSYGG PHPHHSVPHG PLPPGMPMPS LGPFGLPHGL EAVGFSQGMW 70 80 90 100 110 120 GVNTRKQRRE RTTFTRAQLD VLEALFGKTR YPDIFMREEV ALKINLPESR VQVWFKNRRA 130 140 150 160 170 180 KCRQQLQQQQ QSNSLSSSKN ASGGGSGNSC SSSSANSRSN SNNNGSSSNN NTQSSGGNNS 190 200 210 220 230 240 NKSSQKQGNS QSSQQGGGSS GGNNSNNNSA AAAASAAAAV AAAQSIKTHH SSFLSAAAAA 250 260 270 280 290 300 ASGGTNQSAN NNSNNNNQGN STPNSSSSGG GGGSQAGGHL SAAAAAAALN VTAAHQNSSP 310 320 330 340 350 360 LLPTPATSVS PVSIVCKKEH LSGGYGSSVG GGGGGGGASS GGLNLGVGVG VGVGVGVGVS 370 380 390 400 410 420 QDLLRSPYDQ LKDAGGDIGA GVHHHHSIYG SAAGSNPRLL QPGGNITPMD SSSSITTPSP 430 440 450 460 470 480 PITPMSPQSA AAAAHAAQSA QSAHHSAAHS AAYMSNHDSY NFWHNQYQQY PNNYAQAPSY 490 500 510 520 530 540 YSQMEYFSNQ NQVNYNMGHS GYTASNFGLS PSPSFTGTVS AQAFSQNSLD YMSPQDKYAN MV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The orthodenticle gene encodes a novel homeo domain protein involved in the development of the Drosophila nervous system and ocellar visual structures." Finkelstein R., Smouse D., Capaci T.M., Spradling A.C., Perrimon N. Genes Dev. 4:1516-1527(1990) [PubMed: 1979296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"orthodenticle is required for photoreceptor cell development in the Drosophila eye." Vandendries E.R., Johnson D., Reinke R. Dev. Biol. 173:243-255(1996) [PubMed: 8575625] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[6]	"Otd/Crx, a dual regulator for the specification of ommatidia subtypes in the Drosophila retina." Tahayato A., Sonneville R., Pichaud F., Wernet M.F., Papatsenko D., Beaufils P., Cook T., Desplan C. Dev. Cell 5:391-402(2003) [PubMed: 12967559] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X58983 mRNA. Translation: CAA41732.1. Frameshift. AE014298 Genomic DNA. Translation: AAX52481.2. BT011185 mRNA. Translation: AAR88546.1.
PIR	A35912.
RefSeq	NP_001014727.2.
UniGene	Dm.2933
3D structure databases
HSSP	HSSP built from PDB template 1FJL based on UniProtKB P06601.
SMR	P22810. Positions 67-129.
ModBase	Search...
Protein-protein interaction databases
IntAct	P22810. 8 interactions.
Genome annotation databases
Ensembl	FBgn0004102. Drosophila melanogaster. [Contig view]
GeneID	31802.
Organism-specific databases
FlyBase	FBgn0004102. oc.
Family and domain databases
InterPro	IPR001356. Homeobox. IPR017970. Homeobox_CS. IPR012287. Homeodomain-rel. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
ProDom	PD000010. Homeobox. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00389. HOX. 1 hit. [Graphical view]
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	775387.

Entry information

Entry name

HMOC_DROME

Accession

Primary (citable) accession number: P22810
Secondary accession number(s): Q59E69, Q6NNU8, Q9W3D0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	April 14, 2009
Last modified:	June 16, 2009
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project