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Protein

Homeobox protein vnd

Gene

vnd

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable transcriptional regulator involved in the regulation of the proneural AS-C genes and the neurogenic genes of the enhancer of split complex. Could specifically activate proneural genes in the ventral-most neuroectoderm.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi545 – 60460HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: FlyBase
  • sequence-specific DNA binding Source: InterPro
  • transcription factor binding Source: FlyBase

GO - Biological processi

  • brain development Source: FlyBase
  • central nervous system development Source: FlyBase
  • dorsal/ventral pattern formation Source: FlyBase
  • ectoderm development Source: FlyBase
  • glial cell development Source: FlyBase
  • negative regulation of gene expression Source: FlyBase
  • negative regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • neuroblast development Source: FlyBase
  • neuroblast fate determination Source: FlyBase
  • pattern specification process Source: FlyBase
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • regulation of transcription, DNA-templated Source: FlyBase
  • regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
  • ventral cord development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein vnd
Alternative name(s):
Homeobox protein NK-2
Protein ventral nervous system defective
Gene namesi
Name:vnd
Synonyms:NK2
ORF Names:CG6172
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0261930. vnd.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi579 – 5791A → T in vnd-29; disrupts neuroblast development. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 723723Homeobox protein vndPRO_0000049071Add
BLAST

Proteomic databases

PaxDbiP22808.

Expressioni

Tissue specificityi

Expressed in the CNS and midgut.1 Publication

Gene expression databases

BgeeiFBgn0261930.
ExpressionAtlasiP22808. differential.
GenevisibleiP22808. DM.

Interactioni

GO - Molecular functioni

  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi57572. 14 interactions.
DIPiDIP-60882N.
IntActiP22808. 2 interactions.
MINTiMINT-283656.
STRINGi7227.FBpp0070105.

Structurei

Secondary structure

1
723
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi542 – 5454Combined sources
Helixi554 – 56613Combined sources
Helixi572 – 58110Combined sources
Helixi586 – 60621Combined sources
Beta strandi608 – 6114Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NK2NMR-P538-613[»]
1NK3NMR-P538-606[»]
1QRYNMR-A538-613[»]
1VNDNMR-A538-613[»]
ProteinModelPortaliP22808.
SMRiP22808. Positions 544-606.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22808.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi225 – 350126His-richAdd
BLAST
Compositional biasi319 – 36042Ala-richAdd
BLAST
Compositional biasi483 – 49311Asn-richAdd
BLAST
Compositional biasi664 – 72057Ala-richAdd
BLAST
Compositional biasi675 – 71541His-richAdd
BLAST

Sequence similaritiesi

Belongs to the NK-2 homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0842. Eukaryota.
ENOG410XR21. LUCA.
GeneTreeiENSGT00840000129671.
InParanoidiP22808.
KOiK08029.
OMAiSGRSAWI.
OrthoDBiEOG091G0LZ2.
PhylomeDBiP22808.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22808-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSASLERT PSKRDRDRER DNSSGLGSAG SLPASPQSAI TVSPSSPATP
60 70 80 90 100
KRPLRTSTPS LERKREREDR EDREDRKERQ ERHERDRDHE RFAAVFSTAS
110 120 130 140 150
TTVPTNTSSS SGLAPEQLRI PTGAAAFSGF PGLHSMSSLM LPSSAAVAAA
160 170 180 190 200
AAAPFLPWSP ILLPPWNHAL LPAAFYPAAL RNALPGLFDA KVPSSQRSGF
210 220 230 240 250
HISDILNLEG SELKNAAAAA AAAAHHGSDL SHHSASESTS GHRGQGSHTS
260 270 280 290 300
PSALSPTPAG VSADEHHNGS GTGGGAGEAD HHSTTEHHAP PSHPQQQHPH
310 320 330 340 350
HQQHHHPHLL LPQQHHQQAV APLPLAHHQS GEAQSHAHAN AAAAHLLASH
360 370 380 390 400
NAAAAAAVAA GQYLPNLPKN FPGSFGDEMS SYHHMAQTML QHSGRSAWIK
410 420 430 440 450
ENELYGTQQP ASPDSTSPVT SEVSYTYIGS NCQTSPALSG DYKSYSRSAD
460 470 480 490 500
SDALSVGDAL HTLHGSSGNG SAGGAPTAHA LHNNNNNTTN NNNHSLKAEG
510 520 530 540 550
INGAGSGHDD SLNEDGIEED IDDVDDADGS GGGDANGSDG LPNKKRKRRV
560 570 580 590 600
LFTKAQTYEL ERRFRQQRYL SAPEREHLAS LIRLTPTQVK IWFQNHRYKT
610 620 630 640 650
KRAQNEKGYE GHPGLLHGHA THPHHPSALP SPRRVAVPVL VRNGKPCLGD
660 670 680 690 700
SSKLGADCVS VSSATATAMQ NAAAHHLVAL NGAAAYQHAA AAAAGLHAHA
710 720
HAHAHAHGHG HPHAHAQRAA WWP
Length:723
Mass (Da):76,422
Last modified:May 10, 2004 - v3
Checksum:i811F20B03A93C95E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 521R → A in AAB34960 (PubMed:7625694).Curated
Sequence conflicti55 – 551R → P in AAB34960 (PubMed:7625694).Curated
Sequence conflicti88 – 881D → E in CAA60619 (PubMed:7628450).Curated
Sequence conflicti149 – 1491A → R in CAA60619 (PubMed:7628450).Curated
Sequence conflicti153 – 1531Missing in CAA60619 (PubMed:7628450).Curated
Sequence conflicti254 – 2541L → V in AAB34960 (PubMed:7625694).Curated
Sequence conflicti362 – 3621Q → K in CAA60619 (PubMed:7628450).Curated
Sequence conflicti399 – 3991I → M in CAA60619 (PubMed:7628450).Curated
Sequence conflicti405 – 4051Y → H in CAA21410 (PubMed:10731137).Curated
Sequence conflicti610 – 6101E → D in CAA21410 (PubMed:10731137).Curated
Sequence conflicti633 – 6342RR → VG (PubMed:2573058).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87141 mRNA. Translation: CAA60619.1.
S78691 mRNA. Translation: AAB34960.1.
AE014298 Genomic DNA. Translation: AAF45521.1.
AL031883 Genomic DNA. Translation: CAA21410.1.
M27290 Genomic DNA. Translation: AAA28617.1.
PIRiS57246.
RefSeqiNP_476786.2. NM_057438.4.

Genome annotation databases

EnsemblMetazoaiFBtr0070110; FBpp0070105; FBgn0261930.
GeneIDi31003.
KEGGidme:Dmel_CG6172.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X87141 mRNA. Translation: CAA60619.1.
S78691 mRNA. Translation: AAB34960.1.
AE014298 Genomic DNA. Translation: AAF45521.1.
AL031883 Genomic DNA. Translation: CAA21410.1.
M27290 Genomic DNA. Translation: AAA28617.1.
PIRiS57246.
RefSeqiNP_476786.2. NM_057438.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NK2NMR-P538-613[»]
1NK3NMR-P538-606[»]
1QRYNMR-A538-613[»]
1VNDNMR-A538-613[»]
ProteinModelPortaliP22808.
SMRiP22808. Positions 544-606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi57572. 14 interactions.
DIPiDIP-60882N.
IntActiP22808. 2 interactions.
MINTiMINT-283656.
STRINGi7227.FBpp0070105.

Proteomic databases

PaxDbiP22808.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0070110; FBpp0070105; FBgn0261930.
GeneIDi31003.
KEGGidme:Dmel_CG6172.

Organism-specific databases

CTDi31003.
FlyBaseiFBgn0261930. vnd.

Phylogenomic databases

eggNOGiKOG0842. Eukaryota.
ENOG410XR21. LUCA.
GeneTreeiENSGT00840000129671.
InParanoidiP22808.
KOiK08029.
OMAiSGRSAWI.
OrthoDBiEOG091G0LZ2.
PhylomeDBiP22808.

Miscellaneous databases

EvolutionaryTraceiP22808.
GenomeRNAii31003.
PROiP22808.

Gene expression databases

BgeeiFBgn0261930.
ExpressionAtlasiP22808. differential.
GenevisibleiP22808. DM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVND_DROME
AccessioniPrimary (citable) accession number: P22808
Secondary accession number(s): Q24589
, Q26436, Q9UB43, Q9W5F0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: May 10, 2004
Last modified: September 7, 2016
This is version 156 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.