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P22803

- TRX2_YEAST

UniProt

P22803 - TRX2_YEAST

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Protein

Thioredoxin-2

Gene

TRX2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Participates as a hydrogen donor in redox reactions through the reversible oxidation of its active center dithiol to a disulfide, accompanied by the transfer of 2 electrons and 2 protons. It is involved in many cellular processes, including deoxyribonucleotide synthesis, repair of oxidatively damaged proteins, protein folding, sulfur metabolism, and redox homeostasis. Thioredoxin-dependent enzymes include phosphoadenosine-phosphosulfate reductase MET16, alkyl-hydroperoxide reductase DOT5, thioredoxin peroxidases TSA1 and TSA2, alkyl hydroperoxide reductase AHP1, and peroxiredoxin HYR1. Thioredoxin is also involved in protection against reducing stress. As part of the LMA1 complex, it is involved in the facilitation of vesicle fusion such as homotypic vacuole and ER-derived COPII vesicle fusion with the Golgi. This activity does not require the redox mechanism. Through its capacity to inactivate the stress response transcription factor YAP1 and its regulator the hydroperoxide stress sensor HYR1, it is involved in feedback regulation of stress response gene expression upon oxidative stress.9 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei25 – 251Deprotonates C-terminal active site Cys
Active sitei31 – 311Nucleophile
Sitei32 – 321Contributes to redox potential value
Sitei33 – 331Contributes to redox potential value
Active sitei34 – 341Nucleophile

GO - Molecular functioni

  1. disulfide oxidoreductase activity Source: SGD
  2. protein disulfide oxidoreductase activity Source: InterPro

GO - Biological processi

  1. cell redox homeostasis Source: SGD
  2. deoxyribonucleotide biosynthetic process Source: UniProtKB-KW
  3. ER to Golgi vesicle-mediated transport Source: SGD
  4. glutathione metabolic process Source: SGD
  5. glycerol ether metabolic process Source: InterPro
  6. protein deglutathionylation Source: SGD
  7. protein transport Source: UniProtKB-KW
  8. retrograde vesicle-mediated transport, Golgi to ER Source: SGD
  9. sulfate assimilation Source: SGD
  10. vacuole fusion, non-autophagic Source: SGD
  11. vacuole inheritance Source: SGD
Complete GO annotation...

Keywords - Biological processi

Deoxyribonucleotide synthesis, Electron transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30891-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin-2
Alternative name(s):
Thioredoxin II
Short name:
TR-II
Thioredoxin-1
Gene namesi
Name:TRX2
Synonyms:TRX1
Ordered Locus Names:YGR209C
ORF Names:G7746
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

SGDiS000003441. TRX2.

Subcellular locationi

Cytoplasm 1 Publication. Golgi apparatus membrane 1 Publication; Peripheral membrane protein 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: SGD
  2. fungal-type vacuole Source: SGD
  3. Golgi apparatus Source: UniProtKB-KW
  4. membrane Source: UniProtKB-KW
  5. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 104103Thioredoxin-2PRO_0000120045Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 34Redox-active1 PublicationPROSITE-ProRule annotation
Modified residuei62 – 621Phosphoserine2 Publications

Post-translational modificationi

Reversible disulfide bond formation between Cys-31 and Cys-34, reverted by thioredoxin reductase TRR1 using NADPH as hydrogen donor.

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP22803.
PaxDbiP22803.
PeptideAtlasiP22803.

Expressioni

Inductioni

Strongly induced by hydrogen peroxide and diamide stress in a YAP1- and SKN7-dependent manner. Also induced by reducing stress by DTT.

Gene expression databases

GenevestigatoriP22803.

Interactioni

Subunit structurei

Monomer. Part of the heterodimeric LMA1 complex together with the proteinase inhibitor PBI2. LMA1 binds to the ATPase SEC18.3 Publications

Protein-protein interaction databases

BioGridi33462. 44 interactions.
DIPiDIP-5552N.
IntActiP22803. 9 interactions.
MINTiMINT-474704.
STRINGi4932.YGR209C.

Structurei

Secondary structure

1
104
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53
Helixi8 – 158
Beta strandi19 – 279
Helixi32 – 4716
Beta strandi51 – 577
Turni58 – 603
Helixi62 – 676
Beta strandi72 – 809
Beta strandi83 – 919
Helixi93 – 1019

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FA4X-ray2.38A/B1-104[»]
2HSYNMR-A1-104[»]
3PINX-ray2.70A1-104[»]
4DSSX-ray2.10B1-104[»]
ProteinModelPortaliP22803.
SMRiP22803. Positions 1-104.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22803.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 104103ThioredoxinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the thioredoxin family.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiCOG0526.
GeneTreeiENSGT00530000063008.
HOGENOMiHOG000292977.
InParanoidiP22803.
KOiK03671.
OMAiCKAMEPR.
OrthoDBiEOG71CFZN.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR005746. Thioredoxin.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PANTHERiPTHR10438. PTHR10438. 1 hit.
PfamiPF00085. Thioredoxin. 1 hit.
[Graphical view]
PIRSFiPIRSF000077. Thioredoxin. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01068. thioredoxin. 1 hit.
PROSITEiPS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22803-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVTQLKSASE YDSALASGDK LVVVDFFATW CGPCKMIAPM IEKFAEQYSD
60 70 80 90 100
AAFYKLDVDE VSDVAQKAEV SSMPTLIFYK GGKEVTRVVG ANPAAIKQAI

ASNV
Length:104
Mass (Da):11,204
Last modified:January 23, 2007 - v3
Checksum:i071503762C90D451
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59168 Genomic DNA. Translation: AAA35170.1.
M62648 Genomic DNA. Translation: AAA35178.1.
U40843 Genomic DNA. Translation: AAA85584.1.
Z49133 Genomic DNA. Translation: CAA89002.1.
Z72994 Genomic DNA. Translation: CAA97236.1.
AY557817 Genomic DNA. Translation: AAS56143.1.
BK006941 Genomic DNA. Translation: DAA08303.1.
PIRiS15049. TXBY1.
RefSeqiNP_011725.3. NM_001181338.3.

Genome annotation databases

EnsemblFungiiYGR209C; YGR209C; YGR209C.
GeneIDi853123.
KEGGisce:YGR209C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59168 Genomic DNA. Translation: AAA35170.1 .
M62648 Genomic DNA. Translation: AAA35178.1 .
U40843 Genomic DNA. Translation: AAA85584.1 .
Z49133 Genomic DNA. Translation: CAA89002.1 .
Z72994 Genomic DNA. Translation: CAA97236.1 .
AY557817 Genomic DNA. Translation: AAS56143.1 .
BK006941 Genomic DNA. Translation: DAA08303.1 .
PIRi S15049. TXBY1.
RefSeqi NP_011725.3. NM_001181338.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2FA4 X-ray 2.38 A/B 1-104 [» ]
2HSY NMR - A 1-104 [» ]
3PIN X-ray 2.70 A 1-104 [» ]
4DSS X-ray 2.10 B 1-104 [» ]
ProteinModelPortali P22803.
SMRi P22803. Positions 1-104.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33462. 44 interactions.
DIPi DIP-5552N.
IntActi P22803. 9 interactions.
MINTi MINT-474704.
STRINGi 4932.YGR209C.

Proteomic databases

MaxQBi P22803.
PaxDbi P22803.
PeptideAtlasi P22803.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR209C ; YGR209C ; YGR209C .
GeneIDi 853123.
KEGGi sce:YGR209C.

Organism-specific databases

SGDi S000003441. TRX2.

Phylogenomic databases

eggNOGi COG0526.
GeneTreei ENSGT00530000063008.
HOGENOMi HOG000292977.
InParanoidi P22803.
KOi K03671.
OMAi CKAMEPR.
OrthoDBi EOG71CFZN.

Enzyme and pathway databases

BioCyci YEAST:G3O-30891-MONOMER.

Miscellaneous databases

EvolutionaryTracei P22803.
NextBioi 973163.

Gene expression databases

Genevestigatori P22803.

Family and domain databases

Gene3Di 3.40.30.10. 1 hit.
InterProi IPR005746. Thioredoxin.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view ]
PANTHERi PTHR10438. PTHR10438. 1 hit.
Pfami PF00085. Thioredoxin. 1 hit.
[Graphical view ]
PIRSFi PIRSF000077. Thioredoxin. 1 hit.
SUPFAMi SSF52833. SSF52833. 1 hit.
TIGRFAMsi TIGR01068. thioredoxin. 1 hit.
PROSITEi PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast thioredoxin genes."
    Gan Z.-R.
    J. Biol. Chem. 266:1692-1696(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Thioredoxin deficiency in yeast prolongs S phase and shortens the G1 interval of the cell cycle."
    Muller E.G.D.
    J. Biol. Chem. 266:9194-9202(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Analysis of the 15.6-kb fragment encompassing the ADE3 gene."
    Song J.M., Cheung E., Rabinowitz J.C.
    Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  4. "Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveals 12 ORFs, including CCT, ADE3 and TR-I genes, homologues of the yeast PMT and EF1G genes, of the human and bacterial electron-transferring flavoproteins (beta-chain) and of the Escherichia coli phosphoserine phosphohydrolase, and five new ORFs."
    Guerreiro P., Barreiros T., Soares H., Cyrne L., Maia e Silva A., Rodrigues-Pousada C.
    Yeast 12:273-280(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  8. "Thioredoxin-dependent peroxide reductase from yeast."
    Chae H.Z., Chung S.J., Rhee S.G.
    J. Biol. Chem. 269:27670-27678(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-13, TSA1 DEPENDENCE ON THIOREDOXIN.
    Strain: ATCC 200358 / YNN 295.
  9. "Yeast thioredoxin. Amino-acid sequence around the active-center disulfide of thioredoxin I and II."
    Hall D.E., Baldesten A., Holmgren A., Reichard P.
    Eur. J. Biochem. 23:328-335(1971) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 27-43, REDOX-ACTIVE DISULFIDE BOND.
  10. "Yeast PAPS reductase: properties and requirements of the purified enzyme."
    Schwenn J.D., Krone F.A., Husmann K.
    Arch. Microbiol. 150:313-319(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SULFATE ASSIMILATION AND METHIONINE METABOLISM.
  11. "A heterodimer of thioredoxin and I(B)2 cooperates with Sec18p (NSF) to promote yeast vacuole inheritance."
    Xu Z., Mayer A., Muller E.G.D., Wickner W.
    J. Cell Biol. 136:299-306(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE LMA1 COMPLEX.
  12. "LMA1 binds to vacuoles at Sec18p (NSF), transfers upon ATP hydrolysis to a t-SNARE (Vam3p) complex, and is released during fusion."
    Xu Z., Sato K., Wickner W.
    Cell 93:1125-1134(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION OF THE LMA1 COMPLEX WITH SEC18.
  13. "Distinct physiological functions of thiol peroxidase isoenzymes in Saccharomyces cerevisiae."
    Park S.G., Cha M.-K., Jeong W., Kim I.-H.
    J. Biol. Chem. 275:5723-5732(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DOT5 AND TSA2 DEPENDENCE ON THIOREDOXIN.
  14. "A new antioxidant with alkyl hydroperoxide defense properties in yeast."
    Lee J., Spector D., Godon C., Labarre J., Toledano M.B.
    J. Biol. Chem. 274:4537-4544(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REDUCTION OF AHP1.
  15. "H2O2 sensing through oxidation of the Yap1 transcription factor."
    Delaunay A., Isnard A.D., Toledano M.B.
    EMBO J. 19:5157-5166(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REGULATION OF YAP1.
  16. "A thiol peroxidase is an H2O2 receptor and redox-transducer in gene activation."
    Delaunay A., Pflieger D., Barrault M.-B., Vinh J., Toledano M.B.
    Cell 111:471-481(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REGULATION OF HYR1/GPX3.
  17. "Thioredoxins are required for protection against a reductive stress in the yeast Saccharomyces cerevisiae."
    Trotter E.W., Grant C.M.
    Mol. Microbiol. 46:869-878(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PROTECTION AGAINST REDUCING STRESS.
  18. "Roles of the glutathione- and thioredoxin-dependent reduction systems in the Escherichia coli and Saccharomyces cerevisiae responses to oxidative stress."
    Carmel-Harel O., Storz G.
    Annu. Rev. Microbiol. 54:439-461(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, OXIDATIVE STRESS.
  19. "Role of the glutathione/glutaredoxin and thioredoxin systems in yeast growth and response to stress conditions."
    Grant C.M.
    Mol. Microbiol. 39:533-541(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  20. "Involvement of LMA1 and GATE-16 family members in intracellular membrane dynamics."
    Elazar Z., Scherz-Shouval R., Shorer H.
    Biochim. Biophys. Acta 1641:145-156(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW, FUNCTION OF THE LMA1 COMPLEX IN VESICLE FUSION.
  21. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  22. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  23. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  24. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-62, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  25. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  26. "Crystal structure of the yeast cytoplasmic thioredoxin Trx2."
    Bao R., Chen Y., Tang Y.-J., Janin J., Zhou C.-Z.
    Proteins 66:246-249(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.38 ANGSTROMS), DISULFIDE BOND, SUBUNIT.

Entry informationi

Entry nameiTRX2_YEAST
AccessioniPrimary (citable) accession number: P22803
Secondary accession number(s): D6VUZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 152 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 17237 molecules/cell in log phase SD medium.1 Publication
Yeast has two cytoplasmic thioredoxins, TRX1 and TRX2, and one mitochondrial, TRX3.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3