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P22777

- PAI1_MOUSE

UniProt

P22777 - PAI1_MOUSE

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Protein

Plasminogen activator inhibitor 1

Gene
Serpine1, Mr1, Pai1, Planh1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei369 – 3702Reactive bond

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. serine-type endopeptidase inhibitor activity Source: MGI

GO - Biological processi

  1. defense response to Gram-negative bacterium Source: BHF-UCL
  2. negative regulation of endopeptidase activity Source: GOC
  3. negative regulation of plasminogen activation Source: BHF-UCL
  4. negative regulation of vascular wound healing Source: BHF-UCL
  5. positive regulation of angiogenesis Source: BHF-UCL
  6. regulation of angiogenesis Source: MGI
  7. regulation of cell proliferation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI04.020.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasminogen activator inhibitor 1
Short name:
PAI
Short name:
PAI-1
Alternative name(s):
Endothelial plasminogen activator inhibitor
Serpin E1
Gene namesi
Name:Serpine1
Synonyms:Mr1, Pai1, Planh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:97608. Serpine1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 InferredAdd
BLAST
Chaini23 – 402380Plasminogen activator inhibitor 1PRO_0000032500Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi232 – 2321N-linked (GlcNAc...) Reviewed prediction
Glycosylationi288 – 2881N-linked (GlcNAc...) Reviewed prediction
Glycosylationi352 – 3521N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP22777.
PRIDEiP22777.

PTM databases

PhosphoSiteiP22777.

Expressioni

Inductioni

Expression in the liver oscillates in a circadian manner.1 Publication

Gene expression databases

CleanExiMM_MR1.
GenevestigatoriP22777.

Interactioni

Subunit structurei

Forms protease inhibiting heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
Tmprss11eQ5S2482EBI-490898,EBI-490889

Protein-protein interaction databases

IntActiP22777. 1 interaction.

Structurei

Secondary structure

1
402
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi31 – 5020
Beta strandi55 – 573
Helixi59 – 7214
Helixi75 – 8511
Helixi94 – 10613
Helixi108 – 1103
Beta strandi111 – 12313
Helixi132 – 1409
Beta strandi145 – 1473
Helixi152 – 16615
Turni167 – 1693
Beta strandi186 – 20015
Helixi204 – 2063
Beta strandi208 – 2103
Beta strandi222 – 23716
Beta strandi243 – 26523
Helixi271 – 2744
Helixi279 – 28810
Beta strandi290 – 29910
Beta strandi301 – 3088
Helixi310 – 3156
Helixi320 – 3223
Turni324 – 3263
Turni330 – 3323
Beta strandi334 – 3363
Beta strandi344 – 3518
Beta strandi353 – 36513
Beta strandi381 – 3877
Turni388 – 3914
Beta strandi392 – 3998

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LW2X-ray1.93A24-402[»]
ProteinModelPortaliP22777.
SMRiP22777. Positions 30-402.

Miscellaneous databases

EvolutionaryTraceiP22777.

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4826.
HOGENOMiHOG000238519.
HOVERGENiHBG106493.
InParanoidiP22777.
PhylomeDBiP22777.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22777-1 [UniParc]FASTAAdd to Basket

« Hide

MQMSSALACL ILGLVLVSGK GFTLPLRESH TAHQATDFGV KVFQQVVQAS    50
KDRNVVFSPY GVSSVLAMLQ MTTAGKTRRQ IQDAMGFKVN EKGTAHALRQ 100
LSKELMGPWN KNEISTADAI FVQRDLELVQ GFMPHFFKLF QTMVKQVDFS 150
EVERARFIIN DWVERHTKGM INDLLAKGAV DELTRLVLVN ALYFSGQWKT 200
PFLEASTHQR LFHKSDGSTV SVPMMAQSNK FNYTEFTTPD GLEYDVVELP 250
YQRDTLSMFI AAPFEKDVHL SALTNILDAE LIRQWKGNMT RLPRLLILPK 300
FSLETEVDLR GPLEKLGMPD MFSATLADFT SLSDQEQLSV AQALQKVRIE 350
VNESGTVASS STAFVISARM APTEMVIDRS FLFVVRHNPT ETILFMGQVM 400
EP 402
Length:402
Mass (Da):45,170
Last modified:August 1, 1991 - v1
Checksum:i765FF1659C70F68C
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231T → M AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33960 mRNA. Translation: AAA39887.1.
CCDSiCCDS19761.1.
PIRiA34761.
UniGeneiMm.250422.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33960 mRNA. Translation: AAA39887.1 .
CCDSi CCDS19761.1.
PIRi A34761.
UniGenei Mm.250422.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3LW2 X-ray 1.93 A 24-402 [» ]
ProteinModelPortali P22777.
SMRi P22777. Positions 30-402.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P22777. 1 interaction.

Protein family/group databases

MEROPSi I04.020.

PTM databases

PhosphoSitei P22777.

Proteomic databases

PaxDbi P22777.
PRIDEi P22777.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:97608. Serpine1.

Phylogenomic databases

eggNOGi COG4826.
HOGENOMi HOG000238519.
HOVERGENi HBG106493.
InParanoidi P22777.
PhylomeDBi P22777.

Miscellaneous databases

EvolutionaryTracei P22777.
PROi P22777.
SOURCEi Search...

Gene expression databases

CleanExi MM_MR1.
Genevestigatori P22777.

Family and domain databases

InterProi IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view ]
PANTHERi PTHR11461. PTHR11461. 1 hit.
Pfami PF00079. Serpin. 1 hit.
[Graphical view ]
SMARTi SM00093. SERPIN. 1 hit.
[Graphical view ]
SUPFAMi SSF56574. SSF56574. 1 hit.
PROSITEi PS00284. SERPIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The c-myc-regulated gene mr1 encodes plasminogen activator inhibitor 1."
    Prendergast G.C., Diamond L.E., Dahl D., Cole M.D.
    Mol. Cell. Biol. 10:1265-1269(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Characterization of the murine plasma fibrinolytic system."
    Lijnen H.R., van Hoef B., Beelen V., Collen D.
    Eur. J. Biochem. 224:863-871(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-29.
  3. "The molecular clock regulates circadian transcription of tissue factor gene."
    Oishi K., Koyanagi S., Ohkura N.
    Biochem. Biophys. Res. Commun. 431:332-335(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiPAI1_MOUSE
AccessioniPrimary (citable) accession number: P22777
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: July 9, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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