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Protein

Plasminogen activator inhibitor 1

Gene

Serpine1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair (By similarity). Involved in cellular and replicative senescence (PubMed:16862142). Plays a role in alveolar type 2 cells senescence in the lung (PubMed:28722352). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei369 – 370Reactive bond2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI04.020

Names & Taxonomyi

Protein namesi
Recommended name:
Plasminogen activator inhibitor 1
Short name:
PAI
Short name:
PAI-1
Alternative name(s):
Endothelial plasminogen activator inhibitor
Serpin E1
Gene namesi
Name:Serpine1
Synonyms:Mr1, Pai1, Planh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97608 Serpine1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000003250023 – 402Plasminogen activator inhibitor 1Add BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi232N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi288N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi352N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP22777
PaxDbiP22777
PeptideAtlasiP22777
PRIDEiP22777

PTM databases

PhosphoSitePlusiP22777

Expressioni

Inductioni

Expression in the liver oscillates in a circadian manner.1 Publication

Gene expression databases

CleanExiMM_MR1

Interactioni

Subunit structurei

Forms a heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Interacts with PPP1CB.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Tmprss11eQ5S2482EBI-490898,EBI-490889

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-492 Vitronectin-PAI-1 complex
CPX-495 uPA-PAI-1 complex
CPX-518 tPA-PAI-1 complex
CORUMiP22777
IntActiP22777, 1 interactor
STRINGi10090.ENSMUSP00000039586

Structurei

Secondary structure

1402
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi31 – 50Combined sources20
Beta strandi55 – 57Combined sources3
Helixi59 – 72Combined sources14
Helixi75 – 85Combined sources11
Helixi94 – 106Combined sources13
Helixi108 – 110Combined sources3
Beta strandi111 – 123Combined sources13
Helixi132 – 140Combined sources9
Beta strandi145 – 147Combined sources3
Helixi152 – 166Combined sources15
Turni167 – 169Combined sources3
Beta strandi186 – 200Combined sources15
Helixi204 – 206Combined sources3
Beta strandi208 – 210Combined sources3
Beta strandi222 – 237Combined sources16
Beta strandi243 – 265Combined sources23
Helixi271 – 274Combined sources4
Helixi279 – 288Combined sources10
Beta strandi290 – 299Combined sources10
Beta strandi301 – 308Combined sources8
Helixi310 – 315Combined sources6
Helixi320 – 322Combined sources3
Turni324 – 326Combined sources3
Turni330 – 332Combined sources3
Beta strandi334 – 336Combined sources3
Beta strandi344 – 351Combined sources8
Beta strandi353 – 365Combined sources13
Beta strandi381 – 387Combined sources7
Turni388 – 391Combined sources4
Beta strandi392 – 399Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LW2X-ray1.93A24-402[»]
ProteinModelPortaliP22777
SMRiP22777
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22777

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2392 Eukaryota
COG4826 LUCA
HOGENOMiHOG000238519
HOVERGENiHBG106493
InParanoidiP22777
PhylomeDBiP22777

Family and domain databases

InterProiView protein in InterPro
IPR023795 Serpin_CS
IPR023796 Serpin_dom
IPR000215 Serpin_fam
IPR036186 Serpin_sf
PANTHERiPTHR11461 PTHR11461, 1 hit
PfamiView protein in Pfam
PF00079 Serpin, 1 hit
SMARTiView protein in SMART
SM00093 SERPIN, 1 hit
SUPFAMiSSF56574 SSF56574, 1 hit
PROSITEiView protein in PROSITE
PS00284 SERPIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22777-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQMSSALACL ILGLVLVSGK GFTLPLRESH TAHQATDFGV KVFQQVVQAS
60 70 80 90 100
KDRNVVFSPY GVSSVLAMLQ MTTAGKTRRQ IQDAMGFKVN EKGTAHALRQ
110 120 130 140 150
LSKELMGPWN KNEISTADAI FVQRDLELVQ GFMPHFFKLF QTMVKQVDFS
160 170 180 190 200
EVERARFIIN DWVERHTKGM INDLLAKGAV DELTRLVLVN ALYFSGQWKT
210 220 230 240 250
PFLEASTHQR LFHKSDGSTV SVPMMAQSNK FNYTEFTTPD GLEYDVVELP
260 270 280 290 300
YQRDTLSMFI AAPFEKDVHL SALTNILDAE LIRQWKGNMT RLPRLLILPK
310 320 330 340 350
FSLETEVDLR GPLEKLGMPD MFSATLADFT SLSDQEQLSV AQALQKVRIE
360 370 380 390 400
VNESGTVASS STAFVISARM APTEMVIDRS FLFVVRHNPT ETILFMGQVM

EP
Length:402
Mass (Da):45,170
Last modified:August 1, 1991 - v1
Checksum:i765FF1659C70F68C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23T → M AA sequence (PubMed:7523120).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33960 mRNA Translation: AAA39887.1
CCDSiCCDS19761.1
PIRiA34761
UniGeneiMm.250422

Similar proteinsi

Entry informationi

Entry nameiPAI1_MOUSE
AccessioniPrimary (citable) accession number: P22777
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: June 20, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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