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P22777

- PAI1_MOUSE

UniProt

P22777 - PAI1_MOUSE

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Protein

Plasminogen activator inhibitor 1

Gene

Serpine1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei369 – 3702Reactive bond

GO - Molecular functioni

  1. serine-type endopeptidase inhibitor activity Source: MGI

GO - Biological processi

  1. defense response to Gram-negative bacterium Source: BHF-UCL
  2. negative regulation of endopeptidase activity Source: GOC
  3. negative regulation of plasminogen activation Source: BHF-UCL
  4. negative regulation of vascular wound healing Source: BHF-UCL
  5. positive regulation of angiogenesis Source: BHF-UCL
  6. regulation of angiogenesis Source: MGI
  7. regulation of cell proliferation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI04.020.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasminogen activator inhibitor 1
Short name:
PAI
Short name:
PAI-1
Alternative name(s):
Endothelial plasminogen activator inhibitor
Serpin E1
Gene namesi
Name:Serpine1
Synonyms:Mr1, Pai1, Planh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:97608. Serpine1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22221 PublicationAdd
BLAST
Chaini23 – 402380Plasminogen activator inhibitor 1PRO_0000032500Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence Analysis
Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP22777.
PaxDbiP22777.
PRIDEiP22777.

PTM databases

PhosphoSiteiP22777.

Expressioni

Inductioni

Expression in the liver oscillates in a circadian manner.1 Publication

Gene expression databases

CleanExiMM_MR1.
GenevestigatoriP22777.

Interactioni

Subunit structurei

Forms protease inhibiting heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Tmprss11eQ5S2482EBI-490898,EBI-490889

Protein-protein interaction databases

IntActiP22777. 1 interaction.

Structurei

Secondary structure

1
402
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi31 – 5020Combined sources
Beta strandi55 – 573Combined sources
Helixi59 – 7214Combined sources
Helixi75 – 8511Combined sources
Helixi94 – 10613Combined sources
Helixi108 – 1103Combined sources
Beta strandi111 – 12313Combined sources
Helixi132 – 1409Combined sources
Beta strandi145 – 1473Combined sources
Helixi152 – 16615Combined sources
Turni167 – 1693Combined sources
Beta strandi186 – 20015Combined sources
Helixi204 – 2063Combined sources
Beta strandi208 – 2103Combined sources
Beta strandi222 – 23716Combined sources
Beta strandi243 – 26523Combined sources
Helixi271 – 2744Combined sources
Helixi279 – 28810Combined sources
Beta strandi290 – 29910Combined sources
Beta strandi301 – 3088Combined sources
Helixi310 – 3156Combined sources
Helixi320 – 3223Combined sources
Turni324 – 3263Combined sources
Turni330 – 3323Combined sources
Beta strandi334 – 3363Combined sources
Beta strandi344 – 3518Combined sources
Beta strandi353 – 36513Combined sources
Beta strandi381 – 3877Combined sources
Turni388 – 3914Combined sources
Beta strandi392 – 3998Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LW2X-ray1.93A24-402[»]
ProteinModelPortaliP22777.
SMRiP22777. Positions 30-402.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22777.

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4826.
HOGENOMiHOG000238519.
HOVERGENiHBG106493.
InParanoidiP22777.
PhylomeDBiP22777.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22777-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQMSSALACL ILGLVLVSGK GFTLPLRESH TAHQATDFGV KVFQQVVQAS
60 70 80 90 100
KDRNVVFSPY GVSSVLAMLQ MTTAGKTRRQ IQDAMGFKVN EKGTAHALRQ
110 120 130 140 150
LSKELMGPWN KNEISTADAI FVQRDLELVQ GFMPHFFKLF QTMVKQVDFS
160 170 180 190 200
EVERARFIIN DWVERHTKGM INDLLAKGAV DELTRLVLVN ALYFSGQWKT
210 220 230 240 250
PFLEASTHQR LFHKSDGSTV SVPMMAQSNK FNYTEFTTPD GLEYDVVELP
260 270 280 290 300
YQRDTLSMFI AAPFEKDVHL SALTNILDAE LIRQWKGNMT RLPRLLILPK
310 320 330 340 350
FSLETEVDLR GPLEKLGMPD MFSATLADFT SLSDQEQLSV AQALQKVRIE
360 370 380 390 400
VNESGTVASS STAFVISARM APTEMVIDRS FLFVVRHNPT ETILFMGQVM

EP
Length:402
Mass (Da):45,170
Last modified:August 1, 1991 - v1
Checksum:i765FF1659C70F68C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231T → M AA sequence (PubMed:7523120)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33960 mRNA. Translation: AAA39887.1.
CCDSiCCDS19761.1.
PIRiA34761.
UniGeneiMm.250422.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33960 mRNA. Translation: AAA39887.1 .
CCDSi CCDS19761.1.
PIRi A34761.
UniGenei Mm.250422.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3LW2 X-ray 1.93 A 24-402 [» ]
ProteinModelPortali P22777.
SMRi P22777. Positions 30-402.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P22777. 1 interaction.

Protein family/group databases

MEROPSi I04.020.

PTM databases

PhosphoSitei P22777.

Proteomic databases

MaxQBi P22777.
PaxDbi P22777.
PRIDEi P22777.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:97608. Serpine1.

Phylogenomic databases

eggNOGi COG4826.
HOGENOMi HOG000238519.
HOVERGENi HBG106493.
InParanoidi P22777.
PhylomeDBi P22777.

Miscellaneous databases

EvolutionaryTracei P22777.
PROi P22777.
SOURCEi Search...

Gene expression databases

CleanExi MM_MR1.
Genevestigatori P22777.

Family and domain databases

InterProi IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view ]
PANTHERi PTHR11461. PTHR11461. 1 hit.
Pfami PF00079. Serpin. 1 hit.
[Graphical view ]
SMARTi SM00093. SERPIN. 1 hit.
[Graphical view ]
SUPFAMi SSF56574. SSF56574. 1 hit.
PROSITEi PS00284. SERPIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The c-myc-regulated gene mr1 encodes plasminogen activator inhibitor 1."
    Prendergast G.C., Diamond L.E., Dahl D., Cole M.D.
    Mol. Cell. Biol. 10:1265-1269(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Characterization of the murine plasma fibrinolytic system."
    Lijnen H.R., van Hoef B., Beelen V., Collen D.
    Eur. J. Biochem. 224:863-871(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 23-29.
  3. "The molecular clock regulates circadian transcription of tissue factor gene."
    Oishi K., Koyanagi S., Ohkura N.
    Biochem. Biophys. Res. Commun. 431:332-335(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiPAI1_MOUSE
AccessioniPrimary (citable) accession number: P22777
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 26, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3