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P22777

- PAI1_MOUSE

UniProt

P22777 - PAI1_MOUSE

Protein

Plasminogen activator inhibitor 1

Gene

Serpine1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei369 – 3702Reactive bond

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. serine-type endopeptidase inhibitor activity Source: MGI

    GO - Biological processi

    1. defense response to Gram-negative bacterium Source: BHF-UCL
    2. negative regulation of endopeptidase activity Source: GOC
    3. negative regulation of plasminogen activation Source: BHF-UCL
    4. negative regulation of vascular wound healing Source: BHF-UCL
    5. positive regulation of angiogenesis Source: BHF-UCL
    6. regulation of angiogenesis Source: MGI
    7. regulation of cell proliferation Source: MGI

    Keywords - Molecular functioni

    Protease inhibitor, Serine protease inhibitor

    Protein family/group databases

    MEROPSiI04.020.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Plasminogen activator inhibitor 1
    Short name:
    PAI
    Short name:
    PAI-1
    Alternative name(s):
    Endothelial plasminogen activator inhibitor
    Serpin E1
    Gene namesi
    Name:Serpine1
    Synonyms:Mr1, Pai1, Planh1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:97608. Serpine1.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: RefGenome

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 22221 PublicationAdd
    BLAST
    Chaini23 – 402380Plasminogen activator inhibitor 1PRO_0000032500Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP22777.
    PRIDEiP22777.

    PTM databases

    PhosphoSiteiP22777.

    Expressioni

    Inductioni

    Expression in the liver oscillates in a circadian manner.1 Publication

    Gene expression databases

    CleanExiMM_MR1.
    GenevestigatoriP22777.

    Interactioni

    Subunit structurei

    Forms protease inhibiting heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Tmprss11eQ5S2482EBI-490898,EBI-490889

    Protein-protein interaction databases

    IntActiP22777. 1 interaction.

    Structurei

    Secondary structure

    1
    402
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi31 – 5020
    Beta strandi55 – 573
    Helixi59 – 7214
    Helixi75 – 8511
    Helixi94 – 10613
    Helixi108 – 1103
    Beta strandi111 – 12313
    Helixi132 – 1409
    Beta strandi145 – 1473
    Helixi152 – 16615
    Turni167 – 1693
    Beta strandi186 – 20015
    Helixi204 – 2063
    Beta strandi208 – 2103
    Beta strandi222 – 23716
    Beta strandi243 – 26523
    Helixi271 – 2744
    Helixi279 – 28810
    Beta strandi290 – 29910
    Beta strandi301 – 3088
    Helixi310 – 3156
    Helixi320 – 3223
    Turni324 – 3263
    Turni330 – 3323
    Beta strandi334 – 3363
    Beta strandi344 – 3518
    Beta strandi353 – 36513
    Beta strandi381 – 3877
    Turni388 – 3914
    Beta strandi392 – 3998

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3LW2X-ray1.93A24-402[»]
    ProteinModelPortaliP22777.
    SMRiP22777. Positions 30-402.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP22777.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the serpin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4826.
    HOGENOMiHOG000238519.
    HOVERGENiHBG106493.
    InParanoidiP22777.
    PhylomeDBiP22777.

    Family and domain databases

    InterProiIPR023795. Serpin_CS.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view]
    PANTHERiPTHR11461. PTHR11461. 1 hit.
    PfamiPF00079. Serpin. 1 hit.
    [Graphical view]
    SMARTiSM00093. SERPIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF56574. SSF56574. 1 hit.
    PROSITEiPS00284. SERPIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22777-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQMSSALACL ILGLVLVSGK GFTLPLRESH TAHQATDFGV KVFQQVVQAS    50
    KDRNVVFSPY GVSSVLAMLQ MTTAGKTRRQ IQDAMGFKVN EKGTAHALRQ 100
    LSKELMGPWN KNEISTADAI FVQRDLELVQ GFMPHFFKLF QTMVKQVDFS 150
    EVERARFIIN DWVERHTKGM INDLLAKGAV DELTRLVLVN ALYFSGQWKT 200
    PFLEASTHQR LFHKSDGSTV SVPMMAQSNK FNYTEFTTPD GLEYDVVELP 250
    YQRDTLSMFI AAPFEKDVHL SALTNILDAE LIRQWKGNMT RLPRLLILPK 300
    FSLETEVDLR GPLEKLGMPD MFSATLADFT SLSDQEQLSV AQALQKVRIE 350
    VNESGTVASS STAFVISARM APTEMVIDRS FLFVVRHNPT ETILFMGQVM 400
    EP 402
    Length:402
    Mass (Da):45,170
    Last modified:August 1, 1991 - v1
    Checksum:i765FF1659C70F68C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti23 – 231T → M AA sequence (PubMed:7523120)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33960 mRNA. Translation: AAA39887.1.
    CCDSiCCDS19761.1.
    PIRiA34761.
    UniGeneiMm.250422.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33960 mRNA. Translation: AAA39887.1 .
    CCDSi CCDS19761.1.
    PIRi A34761.
    UniGenei Mm.250422.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3LW2 X-ray 1.93 A 24-402 [» ]
    ProteinModelPortali P22777.
    SMRi P22777. Positions 30-402.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P22777. 1 interaction.

    Protein family/group databases

    MEROPSi I04.020.

    PTM databases

    PhosphoSitei P22777.

    Proteomic databases

    PaxDbi P22777.
    PRIDEi P22777.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:97608. Serpine1.

    Phylogenomic databases

    eggNOGi COG4826.
    HOGENOMi HOG000238519.
    HOVERGENi HBG106493.
    InParanoidi P22777.
    PhylomeDBi P22777.

    Miscellaneous databases

    EvolutionaryTracei P22777.
    PROi P22777.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_MR1.
    Genevestigatori P22777.

    Family and domain databases

    InterProi IPR023795. Serpin_CS.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view ]
    PANTHERi PTHR11461. PTHR11461. 1 hit.
    Pfami PF00079. Serpin. 1 hit.
    [Graphical view ]
    SMARTi SM00093. SERPIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56574. SSF56574. 1 hit.
    PROSITEi PS00284. SERPIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The c-myc-regulated gene mr1 encodes plasminogen activator inhibitor 1."
      Prendergast G.C., Diamond L.E., Dahl D., Cole M.D.
      Mol. Cell. Biol. 10:1265-1269(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Characterization of the murine plasma fibrinolytic system."
      Lijnen H.R., van Hoef B., Beelen V., Collen D.
      Eur. J. Biochem. 224:863-871(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 23-29.
    3. "The molecular clock regulates circadian transcription of tissue factor gene."
      Oishi K., Koyanagi S., Ohkura N.
      Biochem. Biophys. Res. Commun. 431:332-335(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiPAI1_MOUSE
    AccessioniPrimary (citable) accession number: P22777
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3