Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P22777 (PAI1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Plasminogen activator inhibitor 1

Short name=PAI
Short name=PAI-1
Alternative name(s):
Endothelial plasminogen activator inhibitor
Serpin E1
Gene names
Name:Serpine1
Synonyms:Mr1, Pai1, Planh1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length402 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis By similarity.

Subunit structure

Forms protease inhibiting heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation By similarity.

Subcellular location

Secreted.

Induction

Expression in the liver oscillates in a circadian manner. Ref.3

Sequence similarities

Belongs to the serpin family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Tmprss11eQ5S2482EBI-490898,EBI-490889

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Probable
Chain23 – 402380Plasminogen activator inhibitor 1
PRO_0000032500

Sites

Site369 – 3702Reactive bond

Amino acid modifications

Glycosylation2321N-linked (GlcNAc...) Potential
Glycosylation2881N-linked (GlcNAc...) Potential
Glycosylation3521N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict231T → M AA sequence Ref.2

Secondary structure

......................................................... 402
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P22777 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 765FF1659C70F68C

FASTA40245,170
        10         20         30         40         50         60 
MQMSSALACL ILGLVLVSGK GFTLPLRESH TAHQATDFGV KVFQQVVQAS KDRNVVFSPY 

        70         80         90        100        110        120 
GVSSVLAMLQ MTTAGKTRRQ IQDAMGFKVN EKGTAHALRQ LSKELMGPWN KNEISTADAI 

       130        140        150        160        170        180 
FVQRDLELVQ GFMPHFFKLF QTMVKQVDFS EVERARFIIN DWVERHTKGM INDLLAKGAV 

       190        200        210        220        230        240 
DELTRLVLVN ALYFSGQWKT PFLEASTHQR LFHKSDGSTV SVPMMAQSNK FNYTEFTTPD 

       250        260        270        280        290        300 
GLEYDVVELP YQRDTLSMFI AAPFEKDVHL SALTNILDAE LIRQWKGNMT RLPRLLILPK 

       310        320        330        340        350        360 
FSLETEVDLR GPLEKLGMPD MFSATLADFT SLSDQEQLSV AQALQKVRIE VNESGTVASS 

       370        380        390        400 
STAFVISARM APTEMVIDRS FLFVVRHNPT ETILFMGQVM EP 

« Hide

References

[1]"The c-myc-regulated gene mr1 encodes plasminogen activator inhibitor 1."
Prendergast G.C., Diamond L.E., Dahl D., Cole M.D.
Mol. Cell. Biol. 10:1265-1269(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Characterization of the murine plasma fibrinolytic system."
Lijnen H.R., van Hoef B., Beelen V., Collen D.
Eur. J. Biochem. 224:863-871(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 23-29.
[3]"The molecular clock regulates circadian transcription of tissue factor gene."
Oishi K., Koyanagi S., Ohkura N.
Biochem. Biophys. Res. Commun. 431:332-335(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M33960 mRNA. Translation: AAA39887.1.
CCDSCCDS19761.1.
PIRA34761.
UniGeneMm.250422.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3LW2X-ray1.93A24-402[»]
ProteinModelPortalP22777.
SMRP22777. Positions 30-402.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP22777. 1 interaction.

Protein family/group databases

MEROPSI04.020.

PTM databases

PhosphoSiteP22777.

Proteomic databases

PaxDbP22777.
PRIDEP22777.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:97608. Serpine1.

Phylogenomic databases

eggNOGCOG4826.
HOGENOMHOG000238519.
HOVERGENHBG106493.
InParanoidP22777.
PhylomeDBP22777.

Gene expression databases

CleanExMM_MR1.
GenevestigatorP22777.

Family and domain databases

InterProIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. SSF56574. 1 hit.
PROSITEPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP22777.
PROP22777.
SOURCESearch...

Entry information

Entry namePAI1_MOUSE
AccessionPrimary (citable) accession number: P22777
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: July 9, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot