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Protein

Plasminogen activator inhibitor 1

Gene

Serpine1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei369 – 370Reactive bond2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI04.020.

Names & Taxonomyi

Protein namesi
Recommended name:
Plasminogen activator inhibitor 1
Short name:
PAI
Short name:
PAI-1
Alternative name(s):
Endothelial plasminogen activator inhibitor
Serpin E1
Gene namesi
Name:Serpine1
Synonyms:Mr1, Pai1, Planh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97608. Serpine1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000003250023 – 402Plasminogen activator inhibitor 1Add BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi232N-linked (GlcNAc...)Sequence analysis1
Glycosylationi288N-linked (GlcNAc...)Sequence analysis1
Glycosylationi352N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP22777.
PaxDbiP22777.
PeptideAtlasiP22777.
PRIDEiP22777.

PTM databases

PhosphoSitePlusiP22777.

Expressioni

Inductioni

Expression in the liver oscillates in a circadian manner.1 Publication

Gene expression databases

CleanExiMM_MR1.

Interactioni

Subunit structurei

Forms protease inhibiting heterodimer with TMPRSS7. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Tmprss11eQ5S2482EBI-490898,EBI-490889

GO - Molecular functioni

Protein-protein interaction databases

IntActiP22777. 1 interactor.
STRINGi10090.ENSMUSP00000039586.

Structurei

Secondary structure

1402
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi31 – 50Combined sources20
Beta strandi55 – 57Combined sources3
Helixi59 – 72Combined sources14
Helixi75 – 85Combined sources11
Helixi94 – 106Combined sources13
Helixi108 – 110Combined sources3
Beta strandi111 – 123Combined sources13
Helixi132 – 140Combined sources9
Beta strandi145 – 147Combined sources3
Helixi152 – 166Combined sources15
Turni167 – 169Combined sources3
Beta strandi186 – 200Combined sources15
Helixi204 – 206Combined sources3
Beta strandi208 – 210Combined sources3
Beta strandi222 – 237Combined sources16
Beta strandi243 – 265Combined sources23
Helixi271 – 274Combined sources4
Helixi279 – 288Combined sources10
Beta strandi290 – 299Combined sources10
Beta strandi301 – 308Combined sources8
Helixi310 – 315Combined sources6
Helixi320 – 322Combined sources3
Turni324 – 326Combined sources3
Turni330 – 332Combined sources3
Beta strandi334 – 336Combined sources3
Beta strandi344 – 351Combined sources8
Beta strandi353 – 365Combined sources13
Beta strandi381 – 387Combined sources7
Turni388 – 391Combined sources4
Beta strandi392 – 399Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LW2X-ray1.93A24-402[»]
ProteinModelPortaliP22777.
SMRiP22777.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22777.

Family & Domainsi

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
HOGENOMiHOG000238519.
HOVERGENiHBG106493.
InParanoidiP22777.
PhylomeDBiP22777.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22777-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQMSSALACL ILGLVLVSGK GFTLPLRESH TAHQATDFGV KVFQQVVQAS
60 70 80 90 100
KDRNVVFSPY GVSSVLAMLQ MTTAGKTRRQ IQDAMGFKVN EKGTAHALRQ
110 120 130 140 150
LSKELMGPWN KNEISTADAI FVQRDLELVQ GFMPHFFKLF QTMVKQVDFS
160 170 180 190 200
EVERARFIIN DWVERHTKGM INDLLAKGAV DELTRLVLVN ALYFSGQWKT
210 220 230 240 250
PFLEASTHQR LFHKSDGSTV SVPMMAQSNK FNYTEFTTPD GLEYDVVELP
260 270 280 290 300
YQRDTLSMFI AAPFEKDVHL SALTNILDAE LIRQWKGNMT RLPRLLILPK
310 320 330 340 350
FSLETEVDLR GPLEKLGMPD MFSATLADFT SLSDQEQLSV AQALQKVRIE
360 370 380 390 400
VNESGTVASS STAFVISARM APTEMVIDRS FLFVVRHNPT ETILFMGQVM

EP
Length:402
Mass (Da):45,170
Last modified:August 1, 1991 - v1
Checksum:i765FF1659C70F68C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23T → M AA sequence (PubMed:7523120).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33960 mRNA. Translation: AAA39887.1.
CCDSiCCDS19761.1.
PIRiA34761.
UniGeneiMm.250422.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33960 mRNA. Translation: AAA39887.1.
CCDSiCCDS19761.1.
PIRiA34761.
UniGeneiMm.250422.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LW2X-ray1.93A24-402[»]
ProteinModelPortaliP22777.
SMRiP22777.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP22777. 1 interactor.
STRINGi10090.ENSMUSP00000039586.

Protein family/group databases

MEROPSiI04.020.

PTM databases

PhosphoSitePlusiP22777.

Proteomic databases

MaxQBiP22777.
PaxDbiP22777.
PeptideAtlasiP22777.
PRIDEiP22777.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:97608. Serpine1.

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
HOGENOMiHOG000238519.
HOVERGENiHBG106493.
InParanoidiP22777.
PhylomeDBiP22777.

Miscellaneous databases

EvolutionaryTraceiP22777.
PROiP22777.
SOURCEiSearch...

Gene expression databases

CleanExiMM_MR1.

Family and domain databases

InterProiIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiPF00079. Serpin. 1 hit.
[Graphical view]
SMARTiSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAI1_MOUSE
AccessioniPrimary (citable) accession number: P22777
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.