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P22772

- MTB3_ANEAE

UniProt

P22772 - MTB3_ANEAE

Protein

Modification methylase BanIII

Gene

banIIIM

Organism
Aneurinibacillus aneurinilyticus (Bacillus aneurinolyticus)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence ATCGAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BanIII endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3296. M.BanIII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase BanIII (EC:2.1.1.72)
    Short name:
    M.BanIII
    Alternative name(s):
    Adenine-specific methyltransferase BanIII
    Gene namesi
    Name:banIIIM
    OrganismiAneurinibacillus aneurinilyticus (Bacillus aneurinolyticus)
    Taxonomic identifieri1391 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeAneurinibacillus groupAneurinibacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 580580Modification methylase BanIIIPRO_0000087943Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP22772.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR003356. DNA_methylase_A-5.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    IPR025931. TaqI_C.
    [Graphical view]
    PfamiPF02384. N6_Mtase. 1 hit.
    PF12950. TaqI_C. 1 hit.
    [Graphical view]
    PRINTSiPR00507. N12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P22772-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELTIEEMLI KQKETGAHYT PTDLGDIIAK RLINELKKSG ISGTKKIRGL    50
    DPSCGDGELL LSLNRIAKFN NIDNIELIGI DEDKEAIKEA DFRLNEMGIN 100
    DAKLTAGDFL DMVDLEGNLS LFDDDLSKIE PVDLIIANPP YVRTQVLGAD 150
    RAQKLAKLFN LKGRVDLYHA FLVAMTLQLK PGGLIGVITS NKYLANSSGE 200
    SIRQFLAENY DIIEIMDLGD TKLFSAAVLQ AIFFGRKKVN KGIRQTAPAN 250
    FYKIYEETDP SKTEVSIKFE TLFGLLESSN TGVFNVDEKF YSVSCGKLIV 300
    PDSFKEPWVM ATDEEYNWIT NINNNSYCTI QDLCDVKVGI KTTADKVFIK 350
    STWEELPDEI KPEVEVLKLL ISTDHASKWR PLERIPNQKI LYTHENVNGK 400
    KKAIDFTQYP HALAYLETHR ETLEGRKYVI KAKRNWYEIW VPQNPDHWAL 450
    PKIVFPDISP EPKFFYEDEG CCIDGNCYWI IPKEENNNDI LFLILGISNT 500
    KYMTNYHDIA FNNKLYPGRR RYLTQYVSKY PLPNPEANYS QEIIDLVREL 550
    VFQNPNDERK IEIENQIENL TALAFGVERL 580
    Length:580
    Mass (Da):66,345
    Last modified:August 1, 1991 - v1
    Checksum:i0E4DF7357EC394C7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83417 Genomic DNA. Translation: CAA58443.1. Sequence problems.
    PIRiJH0224.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83417 Genomic DNA. Translation: CAA58443.1 . Sequence problems.
    PIRi JH0224.

    3D structure databases

    ProteinModelPortali P22772.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3296. M.BanIII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR003356. DNA_methylase_A-5.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR029063. SAM-dependent_MTases-like.
    IPR025931. TaqI_C.
    [Graphical view ]
    Pfami PF02384. N6_Mtase. 1 hit.
    PF12950. TaqI_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00507. N12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the gene coding for the BanIII DNA methyltransferase in Bacillus aneurinolyticus."
      Kawakami B., Sasaki A., Oka M., Maekawa Y.
      Agric. Biol. Chem. 54:3227-3233(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiMTB3_ANEAE
    AccessioniPrimary (citable) accession number: P22772
    Secondary accession number(s): Q44656
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3