P22772 (MTB3_BACAR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase BanIII
Short name=M.BanIII
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase BanIII

Gene names

Name:

banIIIM

Organism

Bacillus aneurinolyticus

Taxonomic identifier

1391 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Paenibacillaceae › Aneurinibacillus group › Aneurinibacillus

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence ATCGAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BanIII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 580	580	Modification methylase BanIII		PRO_0000087943

Sequences

	Sequence		Length	Mass (Da)
	P22772 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 0E4DF7357EC394C7	FASTA	580	66,345
	10 20 30 40 50 60 MELTIEEMLI KQKETGAHYT PTDLGDIIAK RLINELKKSG ISGTKKIRGL DPSCGDGELL 70 80 90 100 110 120 LSLNRIAKFN NIDNIELIGI DEDKEAIKEA DFRLNEMGIN DAKLTAGDFL DMVDLEGNLS 130 140 150 160 170 180 LFDDDLSKIE PVDLIIANPP YVRTQVLGAD RAQKLAKLFN LKGRVDLYHA FLVAMTLQLK 190 200 210 220 230 240 PGGLIGVITS NKYLANSSGE SIRQFLAENY DIIEIMDLGD TKLFSAAVLQ AIFFGRKKVN 250 260 270 280 290 300 KGIRQTAPAN FYKIYEETDP SKTEVSIKFE TLFGLLESSN TGVFNVDEKF YSVSCGKLIV 310 320 330 340 350 360 PDSFKEPWVM ATDEEYNWIT NINNNSYCTI QDLCDVKVGI KTTADKVFIK STWEELPDEI 370 380 390 400 410 420 KPEVEVLKLL ISTDHASKWR PLERIPNQKI LYTHENVNGK KKAIDFTQYP HALAYLETHR 430 440 450 460 470 480 ETLEGRKYVI KAKRNWYEIW VPQNPDHWAL PKIVFPDISP EPKFFYEDEG CCIDGNCYWI 490 500 510 520 530 540 IPKEENNNDI LFLILGISNT KYMTNYHDIA FNNKLYPGRR RYLTQYVSKY PLPNPEANYS 550 560 570 580 QEIIDLVREL VFQNPNDERK IEIENQIENL TALAFGVERL

References

[1]	"Nucleotide sequence of the gene coding for the BanIII DNA methyltransferase in Bacillus aneurinolyticus." Kawakami B., Sasaki A., Oka M., Maekawa Y. Agric. Biol. Chem. 54:3227-3233(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X83417 Genomic DNA. Translation: CAA58443.1. Sequence problems.
PIR	JH0224.
3D structure databases
ProteinModelPortal	P22772.
ModBase	Search...
Protein family/group databases
REBASE	3296. M.BanIII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR003356. DNA_methylase_A-5. IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. IPR025931. TaqI_C. [Graphical view]
Pfam	PF02384. N6_Mtase. 1 hit. PF12950. TaqI_C. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTB3_BACAR

Accession

Primary (citable) accession number: P22772
Secondary accession number(s): Q44656

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program