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Protein

Modification methylase BanIII

Gene

banIIIM

Organism
Aneurinibacillus aneurinilyticus (Bacillus aneurinolyticus)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence ATCGAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BanIII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3296. M.BanIII.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BanIII (EC:2.1.1.72)
Short name:
M.BanIII
Alternative name(s):
Adenine-specific methyltransferase BanIII
Gene namesi
Name:banIIIM
OrganismiAneurinibacillus aneurinilyticus (Bacillus aneurinolyticus)
Taxonomic identifieri1391 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeAneurinibacillus groupAneurinibacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 580580Modification methylase BanIIIPRO_0000087943Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP22772.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases.
IPR025931. TaqI_C.
[Graphical view]
PfamiPF02384. N6_Mtase. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22772-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELTIEEMLI KQKETGAHYT PTDLGDIIAK RLINELKKSG ISGTKKIRGL
60 70 80 90 100
DPSCGDGELL LSLNRIAKFN NIDNIELIGI DEDKEAIKEA DFRLNEMGIN
110 120 130 140 150
DAKLTAGDFL DMVDLEGNLS LFDDDLSKIE PVDLIIANPP YVRTQVLGAD
160 170 180 190 200
RAQKLAKLFN LKGRVDLYHA FLVAMTLQLK PGGLIGVITS NKYLANSSGE
210 220 230 240 250
SIRQFLAENY DIIEIMDLGD TKLFSAAVLQ AIFFGRKKVN KGIRQTAPAN
260 270 280 290 300
FYKIYEETDP SKTEVSIKFE TLFGLLESSN TGVFNVDEKF YSVSCGKLIV
310 320 330 340 350
PDSFKEPWVM ATDEEYNWIT NINNNSYCTI QDLCDVKVGI KTTADKVFIK
360 370 380 390 400
STWEELPDEI KPEVEVLKLL ISTDHASKWR PLERIPNQKI LYTHENVNGK
410 420 430 440 450
KKAIDFTQYP HALAYLETHR ETLEGRKYVI KAKRNWYEIW VPQNPDHWAL
460 470 480 490 500
PKIVFPDISP EPKFFYEDEG CCIDGNCYWI IPKEENNNDI LFLILGISNT
510 520 530 540 550
KYMTNYHDIA FNNKLYPGRR RYLTQYVSKY PLPNPEANYS QEIIDLVREL
560 570 580
VFQNPNDERK IEIENQIENL TALAFGVERL
Length:580
Mass (Da):66,345
Last modified:August 1, 1991 - v1
Checksum:i0E4DF7357EC394C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83417 Genomic DNA. Translation: CAA58443.1. Sequence problems.
PIRiJH0224.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83417 Genomic DNA. Translation: CAA58443.1. Sequence problems.
PIRiJH0224.

3D structure databases

ProteinModelPortaliP22772.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3296. M.BanIII.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases.
IPR025931. TaqI_C.
[Graphical view]
PfamiPF02384. N6_Mtase. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the gene coding for the BanIII DNA methyltransferase in Bacillus aneurinolyticus."
    Kawakami B., Sasaki A., Oka M., Maekawa Y.
    Agric. Biol. Chem. 54:3227-3233(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTB3_ANEAE
AccessioniPrimary (citable) accession number: P22772
Secondary accession number(s): Q44656
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: April 29, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.