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P22772 (MTB3_ANEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase BanIII

Short name=M.BanIII
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase BanIII
Gene names
Name:banIIIM
OrganismAneurinibacillus aneurinilyticus (Bacillus aneurinolyticus)
Taxonomic identifier1391 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeAneurinibacillus groupAneurinibacillus

Protein attributes

Sequence length580 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence ATCGAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BanIII endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 580580Modification methylase BanIII
PRO_0000087943

Sequences

Sequence LengthMass (Da)Tools
P22772 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 0E4DF7357EC394C7

FASTA58066,345
        10         20         30         40         50         60 
MELTIEEMLI KQKETGAHYT PTDLGDIIAK RLINELKKSG ISGTKKIRGL DPSCGDGELL 

        70         80         90        100        110        120 
LSLNRIAKFN NIDNIELIGI DEDKEAIKEA DFRLNEMGIN DAKLTAGDFL DMVDLEGNLS 

       130        140        150        160        170        180 
LFDDDLSKIE PVDLIIANPP YVRTQVLGAD RAQKLAKLFN LKGRVDLYHA FLVAMTLQLK 

       190        200        210        220        230        240 
PGGLIGVITS NKYLANSSGE SIRQFLAENY DIIEIMDLGD TKLFSAAVLQ AIFFGRKKVN 

       250        260        270        280        290        300 
KGIRQTAPAN FYKIYEETDP SKTEVSIKFE TLFGLLESSN TGVFNVDEKF YSVSCGKLIV 

       310        320        330        340        350        360 
PDSFKEPWVM ATDEEYNWIT NINNNSYCTI QDLCDVKVGI KTTADKVFIK STWEELPDEI 

       370        380        390        400        410        420 
KPEVEVLKLL ISTDHASKWR PLERIPNQKI LYTHENVNGK KKAIDFTQYP HALAYLETHR 

       430        440        450        460        470        480 
ETLEGRKYVI KAKRNWYEIW VPQNPDHWAL PKIVFPDISP EPKFFYEDEG CCIDGNCYWI 

       490        500        510        520        530        540 
IPKEENNNDI LFLILGISNT KYMTNYHDIA FNNKLYPGRR RYLTQYVSKY PLPNPEANYS 

       550        560        570        580 
QEIIDLVREL VFQNPNDERK IEIENQIENL TALAFGVERL 

« Hide

References

[1]"Nucleotide sequence of the gene coding for the BanIII DNA methyltransferase in Bacillus aneurinolyticus."
Kawakami B., Sasaki A., Oka M., Maekawa Y.
Agric. Biol. Chem. 54:3227-3233(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83417 Genomic DNA. Translation: CAA58443.1. Sequence problems.
PIRJH0224.

3D structure databases

ProteinModelPortalP22772.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3296. M.BanIII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
IPR025931. TaqI_C.
[Graphical view]
PfamPF02384. N6_Mtase. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view]
PRINTSPR00507. N12N6MTFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTB3_ANEAE
AccessionPrimary (citable) accession number: P22772
Secondary accession number(s): Q44656
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: June 11, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries