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Protein

Proteasome subunit alpha type-7-1

Gene

Prosalpha4

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: FlyBase

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-DME-1169091 Activation of NF-kappaB in B cells
R-DME-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-DME-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-DME-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-DME-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-DME-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-DME-187577 SCF(Skp2)-mediated degradation of p27/p21
R-DME-195253 Degradation of beta-catenin by the destruction complex
R-DME-202424 Downstream TCR signaling
R-DME-209360 Ubiquitination and proteolysis of phosphorylated CI
R-DME-209406 Degradation of NF-kappa-B inhibitor, CACT
R-DME-209461 Ubiquitination and degradation of phosphorylated ARM
R-DME-216167 Nuclear CI is degraded
R-DME-2871837 FCERI mediated NF-kB activation
R-DME-432395 Degradation of TIM
R-DME-432524 Degradation of PER
R-DME-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-DME-4608870 Asymmetric localization of PCP proteins
R-DME-4641257 Degradation of AXIN
R-DME-4641258 Degradation of DVL
R-DME-5358346 Hedgehog ligand biogenesis
R-DME-538848 Degradation of CLK
R-DME-538864 Degradation of CRY
R-DME-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-DME-5607764 CLEC7A (Dectin-1) signaling
R-DME-5610785 GLI3 is processed to GLI3R by the proteasome
R-DME-5632684 Hedgehog 'on' state
R-DME-5658442 Regulation of RAS by GAPs
R-DME-5676590 NIK-->noncanonical NF-kB signaling
R-DME-5689603 UCH proteinases
R-DME-5689880 Ub-specific processing proteases
R-DME-68949 Orc1 removal from chromatin
R-DME-69017 CDK-mediated phosphorylation and removal of Cdc6
R-DME-69229 Ubiquitin-dependent degradation of Cyclin D1
R-DME-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-DME-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-DME-8939902 Regulation of RUNX2 expression and activity
R-DME-8941858 Regulation of RUNX3 expression and activity
R-DME-8948751 Regulation of PTEN stability and activity
R-DME-9020702 Interleukin-1 signaling
R-DME-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-7-1 (EC:3.4.25.1)
Alternative name(s):
PROS-Dm28.1
Proteasome 28 kDa subunit 1
Gene namesi
Name:Prosalpha4
Synonyms:PROS-28.1, Pros28.1
ORF Names:CG3422
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0004066 Prosalpha4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001241531 – 249Proteasome subunit alpha type-7-1Add BLAST249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei177Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP22769
PRIDEiP22769

PTM databases

iPTMnetiP22769

Expressioni

Gene expression databases

BgeeiFBgn0004066
GenevisibleiP22769 DM

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity). Interacts with PI31; this interaction is reduced by PI31 ADP-ribosylation.By similarity1 Publication

Protein-protein interaction databases

BioGridi58925, 52 interactors
DIPiDIP-17376N
IntActiP22769, 12 interactors
STRINGi7227.FBpp0073989

Structurei

3D structure databases

ProteinModelPortaliP22769
SMRiP22769
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0183 Eukaryota
ENOG410XP21 LUCA
GeneTreeiENSGT00550000074753
HOGENOMiHOG000072912
InParanoidiP22769
KOiK02731
OMAiEVAIMEN
OrthoDBiEOG091G0GX6
PhylomeDBiP22769

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR023332 Proteasome_alpha-type
IPR037560 Proteasome_alpha_7-like
IPR000426 Proteasome_asu_N
IPR001353 Proteasome_sua/b
PANTHERiPTHR11599:SF47 PTHR11599:SF47, 1 hit
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PF10584 Proteasome_A_N, 1 hit
SMARTiView protein in SMART
SM00948 Proteasome_A_N, 1 hit
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00388 PROTEASOME_ALPHA_1, 1 hit
PS51475 PROTEASOME_ALPHA_2, 1 hit

Sequencei

Sequence statusi: Complete.

P22769-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRYDRAVT IFSPDGHLLQ VEYAQEAVRK GSTAVGVRGA NCVVLGVEKK
60 70 80 90 100
SVAQLQEDRK VRKICMLDNH VVMAFAGLTA DARIMINRAQ VECQSHRLNV
110 120 130 140 150
EDPVTLEYIT RFIAQLKQKY TQSNGRRPFG ISCLIGGFDA DGSAHLFQTE
160 170 180 190 200
PSGIFYEYKA NATGRSAKVV REFFEKSYRE EEVANEHGAV KLAIRALLEV
210 220 230 240
AQSGQNNLEV AIMENGKPLK MLDTDVITDY VKIIEKEKEE ELEKKKQKK
Length:249
Mass (Da):27,973
Last modified:December 8, 2000 - v2
Checksum:i56987932DADD02B3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti224T → N in AAA62768 (PubMed:2169443).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57712 mRNA Translation: AAA62768.1
X62286 Genomic DNA Translation: CAA44174.1
AY542383 Genomic DNA Translation: AAS86210.1
AY542384 Genomic DNA Translation: AAS86211.1
AY542385 Genomic DNA Translation: AAS86212.1
AY542386 Genomic DNA Translation: AAS86213.1
AY542387 Genomic DNA Translation: AAS86214.1
AY542388 Genomic DNA Translation: AAS86215.1
AY542389 Genomic DNA Translation: AAS86216.1
AE014298 Genomic DNA Translation: AAF48573.1
AY071241 mRNA Translation: AAL48863.1
PIRiJQ0681
S23451
RefSeqiNP_525092.1, NM_080353.5
UniGeneiDm.1671

Genome annotation databases

EnsemblMetazoaiFBtr0074210; FBpp0073989; FBgn0004066
GeneIDi32584
KEGGidme:Dmel_CG3422

Similar proteinsi

Entry informationi

Entry nameiPSA71_DROME
AccessioniPrimary (citable) accession number: P22769
Secondary accession number(s): Q9VXI9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: December 8, 2000
Last modified: May 23, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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