Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P22768

- ASSY_YEAST

UniProt

P22768 - ASSY_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Argininosuccinate synthase

Gene

ARG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the eighth step in arginine biosynthesis. Also has a catabolic function as the first enzyme of citrulline utilization as nitrogen source via arginine and the reactions involved in the arginase pathway.1 Publication

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Kineticsi

  1. KM=0.1 mM for L-citrulline1 Publication
  2. KM=0.03 mM for L-aspartate1 Publication
  3. KM=0.3 mM for ATP1 Publication

pH dependencei

Optimum pH is 7.5-8.0 for the forward reaction and 6.0-6.5 for the reverse reaction.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei86 – 861CitrullineBy similarity
Binding sitei91 – 911CitrullineBy similarity
Binding sitei118 – 1181AspartateBy similarity
Binding sitei122 – 1221AspartateBy similarity
Binding sitei122 – 1221CitrullineBy similarity
Binding sitei123 – 1231AspartateBy similarity
Binding sitei126 – 1261CitrullineBy similarity
Binding sitei179 – 1791CitrullineBy similarity
Binding sitei188 – 1881CitrullineBy similarity
Binding sitei273 – 2731CitrullineBy similarity
Binding sitei285 – 2851CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 179ATPBy similarity
Nucleotide bindingi114 – 1229ATPBy similarity

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: SGD
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. arginine biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YOL058W-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:ARG1
Ordered Locus Names:YOL058W
ORF Names:O1228
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOL058w.
SGDiS000005419. ARG1.

Subcellular locationi

Cytoplasm 2 Publications

GO - Cellular componenti

  1. cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 420420Argininosuccinate synthasePRO_0000148559Add
BLAST

Proteomic databases

MaxQBiP22768.
PaxDbiP22768.
PeptideAtlasiP22768.

Expressioni

Gene expression databases

GenevestigatoriP22768.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi34343. 18 interactions.
DIPiDIP-1660N.
IntActiP22768. 4 interactions.
MINTiMINT-393127.
STRINGi4932.YOL058W.

Structurei

3D structure databases

ProteinModelPortaliP22768.
SMRiP22768. Positions 3-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0137.
GeneTreeiENSGT00390000004524.
HOGENOMiHOG000230093.
InParanoidiP22768.
KOiK01940.
OMAiPECEYIR.
OrthoDBiEOG7KSXJR.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22768-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKGKVCLAY SGGLDTSVIL AWLLDQGYEV VAFMANVGQE EDFDAAKEKA
60 70 80 90 100
LKIGACKFVC VDCREDFVKD ILFPAVQVNA VYEDVYLLGT SLARPVIAKA
110 120 130 140 150
QIDVAKQEGC FAVSHGCTGK GNDQIRFELS FYALKPDVKC ITPWRMPEFF
160 170 180 190 200
ERFAGRKDLL DYAAQKGIPV AQTKAKPWST DENQAHISYE AGILEDPDTT
210 220 230 240 250
PPKDMWKLIV DPMDAPDQPQ DLTIDFERGL PVKLTYTDNK TSKEVSVTKP
260 270 280 290 300
LDVFLAASNL ARANGVGRID IVEDRYINLK SRGCYEQAPL TVLRKAHVDL
310 320 330 340 350
EGLTLDKEVR QLRDSFVTPN YSRLIYNGSY FTPECEYIRS MIQPSQNSVN
360 370 380 390 400
GTVRVRLYKG NVIILGRSTK TEKLYDPTES SMDELTGFLP TDTTGFIAIQ
410 420
AIRIKKYGES KKTKGEELTL
Length:420
Mass (Da):46,928
Last modified:September 21, 2011 - v3
Checksum:i155E1A4775B6FA9B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 282GY → AT in AAA34437. (PubMed:2123815)Curated
Sequence conflicti27 – 282GY → AT in CAA30106. (PubMed:2897249)Curated
Sequence conflicti48 – 492EK → VL in AAA34437. (PubMed:2123815)Curated
Sequence conflicti48 – 492EK → VL in CAA30106. (PubMed:2897249)Curated
Sequence conflicti61 – 644VDCR → GGLS in AAA34437. (PubMed:2123815)Curated
Sequence conflicti169 – 1691P → F in AAA34437. (PubMed:2123815)Curated
Sequence conflicti316 – 3161F → L in AAA34437. (PubMed:2123815)Curated
Sequence conflicti329 – 3324SYFT → FLLH in AAA34437. (PubMed:2123815)Curated
Sequence conflicti329 – 3324SYFT → FLLH in CAA62528. (PubMed:8789261)Curated
Sequence conflicti329 – 3324SYFT → FLLH in CAA99067. (PubMed:9169874)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35237 Genomic DNA. Translation: AAA34437.1.
X91067 Genomic DNA. Translation: CAA62528.1.
Z74800 Genomic DNA. Translation: CAA99067.1.
X07070 Genomic DNA. Translation: CAA30106.1.
BK006948 Genomic DNA. Translation: DAA10725.2.
PIRiS59291. AJBYRS.
RefSeqiNP_014583.2. NM_001183313.2.

Genome annotation databases

EnsemblFungiiYOL058W; YOL058W; YOL058W.
GeneIDi854096.
KEGGisce:YOL058W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M35237 Genomic DNA. Translation: AAA34437.1 .
X91067 Genomic DNA. Translation: CAA62528.1 .
Z74800 Genomic DNA. Translation: CAA99067.1 .
X07070 Genomic DNA. Translation: CAA30106.1 .
BK006948 Genomic DNA. Translation: DAA10725.2 .
PIRi S59291. AJBYRS.
RefSeqi NP_014583.2. NM_001183313.2.

3D structure databases

ProteinModelPortali P22768.
SMRi P22768. Positions 3-405.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34343. 18 interactions.
DIPi DIP-1660N.
IntActi P22768. 4 interactions.
MINTi MINT-393127.
STRINGi 4932.YOL058W.

Proteomic databases

MaxQBi P22768.
PaxDbi P22768.
PeptideAtlasi P22768.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOL058W ; YOL058W ; YOL058W .
GeneIDi 854096.
KEGGi sce:YOL058W.

Organism-specific databases

CYGDi YOL058w.
SGDi S000005419. ARG1.

Phylogenomic databases

eggNOGi COG0137.
GeneTreei ENSGT00390000004524.
HOGENOMi HOG000230093.
InParanoidi P22768.
KOi K01940.
OMAi PECEYIR.
OrthoDBi EOG7KSXJR.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00113 .
BioCyci YEAST:YOL058W-MONOMER.

Miscellaneous databases

NextBioi 975761.
PROi P22768.

Gene expression databases

Genevestigatori P22768.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPi MF_00005. Arg_succ_synth_type1.
InterProi IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00764. Arginosuc_synth. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00032. argG. 1 hit.
PROSITEi PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequences of the genes encoding argininosuccinate synthetase in Escherichia coli and Saccharomyces cerevisiae: comparison with methanogenic archaebacteria and mammals."
    van Vliet F., Crabeel M., Boyen A., Tricot C., Stalon V., Falmagne P., Nakamura Y., Baumberg S., Glansdorff N.
    Gene 95:99-104(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of a 26 kb region on the left arm of yeast chromosome XV."
    Mannhaupt G., Vetter I., Schwarzlose C., Mitzel S., Feldmann H.
    Yeast 12:67-76(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 90843 / S288c / FY73.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 329-332.
    Strain: ATCC 204508 / S288c.
  5. "Arginine repression of the Saccharomyces cerevisiae ARG1 gene. Comparison of the ARG1 and ARG3 control regions."
    Crabeel M., Seneca S., Devos K., Glansdorff N.
    Curr. Genet. 13:113-124(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-57.
  6. "Arginine metabolism in Saccharomyces cerevisiae: subcellular localization of the enzymes."
    Jauniaux J.-C., Urrestarazu L.A., Wiame J.-M.
    J. Bacteriol. 133:1096-1107(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "Yeast argininosuccinate synthetase. Purification; structural and kinetic properties."
    Hilger F., Simon J.-P., Stalon V.
    Eur. J. Biochem. 94:153-163(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiASSY_YEAST
AccessioniPrimary (citable) accession number: P22768
Secondary accession number(s): D6W209
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: September 21, 2011
Last modified: October 29, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1870 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3