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Protein

Argininosuccinate synthase

Gene

ARG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the eighth step in arginine biosynthesis. Also has a catabolic function as the first enzyme of citrulline utilization as nitrogen source via arginine and the reactions involved in the arginase pathway.1 Publication

Miscellaneous

Present with 1870 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Kineticsi

  1. KM=0.1 mM for L-citrulline1 Publication
  2. KM=0.03 mM for L-aspartate1 Publication
  3. KM=0.3 mM for ATP1 Publication

    pH dependencei

    Optimum pH is 7.5-8.0 for the forward reaction and 6.0-6.5 for the reverse reaction.1 Publication

    Pathwayi: L-arginine biosynthesis

    This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. Ornithine carbamoyltransferase (ARG3)
    2. Argininosuccinate synthase (ARG1)
    3. Argininosuccinate lyase (ARG4)
    This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei35ATP; via amide nitrogen and carbonyl oxygenBy similarity1
    Binding sitei86CitrullineBy similarity1
    Binding sitei91CitrullineBy similarity1
    Binding sitei118AspartateBy similarity1
    Binding sitei122AspartateBy similarity1
    Binding sitei122CitrullineBy similarity1
    Binding sitei123AspartateBy similarity1
    Binding sitei126CitrullineBy similarity1
    Binding sitei179CitrullineBy similarity1
    Binding sitei188CitrullineBy similarity1
    Binding sitei273CitrullineBy similarity1
    Binding sitei285CitrullineBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi9 – 17ATPBy similarity9
    Nucleotide bindingi114 – 122ATPBy similarity9

    GO - Molecular functioni

    • argininosuccinate synthase activity Source: SGD
    • ATP binding Source: UniProtKB-KW

    GO - Biological processi

    • arginine biosynthetic process Source: SGD
    • argininosuccinate metabolic process Source: GO_Central
    • urea cycle Source: GO_Central

    Keywordsi

    Molecular functionLigase
    Biological processAmino-acid biosynthesis, Arginine biosynthesis
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:YOL058W-MONOMER
    UniPathwayiUPA00068; UER00113

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Argininosuccinate synthase (EC:6.3.4.5)
    Alternative name(s):
    Citrulline--aspartate ligase
    Gene namesi
    Name:ARG1
    Ordered Locus Names:YOL058W
    ORF Names:O1228
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XV

    Organism-specific databases

    EuPathDBiFungiDB:YOL058W
    SGDiS000005419 ARG1

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001485591 – 420Argininosuccinate synthaseAdd BLAST420

    Proteomic databases

    MaxQBiP22768
    PaxDbiP22768
    PRIDEiP22768

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    BioGridi34343, 23 interactors
    DIPiDIP-1660N
    IntActiP22768, 5 interactors
    MINTiP22768
    STRINGi4932.YOL058W

    Structurei

    3D structure databases

    ProteinModelPortaliP22768
    SMRiP22768
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00390000004524
    HOGENOMiHOG000230093
    InParanoidiP22768
    KOiK01940
    OMAiGVGRIDM
    OrthoDBiEOG092C2BM5

    Family and domain databases

    CDDicd01999 Argininosuccinate_Synthase, 1 hit
    Gene3Di3.40.50.620, 1 hit
    3.90.1260.10, 1 hit
    HAMAPiMF_00005 Arg_succ_synth_type1, 1 hit
    InterProiView protein in InterPro
    IPR001518 Arginosuc_synth
    IPR018223 Arginosuc_synth_CS
    IPR023434 Arginosuc_synth_type_1_subfam
    IPR024074 AS_cat/multimer_dom_body
    IPR014729 Rossmann-like_a/b/a_fold
    PANTHERiPTHR11587 PTHR11587, 1 hit
    PfamiView protein in Pfam
    PF00764 Arginosuc_synth, 1 hit
    TIGRFAMsiTIGR00032 argG, 1 hit
    PROSITEiView protein in PROSITE
    PS00564 ARGININOSUCCIN_SYN_1, 1 hit
    PS00565 ARGININOSUCCIN_SYN_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P22768-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSKGKVCLAY SGGLDTSVIL AWLLDQGYEV VAFMANVGQE EDFDAAKEKA
    60 70 80 90 100
    LKIGACKFVC VDCREDFVKD ILFPAVQVNA VYEDVYLLGT SLARPVIAKA
    110 120 130 140 150
    QIDVAKQEGC FAVSHGCTGK GNDQIRFELS FYALKPDVKC ITPWRMPEFF
    160 170 180 190 200
    ERFAGRKDLL DYAAQKGIPV AQTKAKPWST DENQAHISYE AGILEDPDTT
    210 220 230 240 250
    PPKDMWKLIV DPMDAPDQPQ DLTIDFERGL PVKLTYTDNK TSKEVSVTKP
    260 270 280 290 300
    LDVFLAASNL ARANGVGRID IVEDRYINLK SRGCYEQAPL TVLRKAHVDL
    310 320 330 340 350
    EGLTLDKEVR QLRDSFVTPN YSRLIYNGSY FTPECEYIRS MIQPSQNSVN
    360 370 380 390 400
    GTVRVRLYKG NVIILGRSTK TEKLYDPTES SMDELTGFLP TDTTGFIAIQ
    410 420
    AIRIKKYGES KKTKGEELTL
    Length:420
    Mass (Da):46,928
    Last modified:September 21, 2011 - v3
    Checksum:i155E1A4775B6FA9B
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti27 – 28GY → AT in AAA34437 (PubMed:2123815).Curated2
    Sequence conflicti27 – 28GY → AT in CAA30106 (PubMed:2897249).Curated2
    Sequence conflicti48 – 49EK → VL in AAA34437 (PubMed:2123815).Curated2
    Sequence conflicti48 – 49EK → VL in CAA30106 (PubMed:2897249).Curated2
    Sequence conflicti61 – 64VDCR → GGLS in AAA34437 (PubMed:2123815).Curated4
    Sequence conflicti169P → F in AAA34437 (PubMed:2123815).Curated1
    Sequence conflicti316F → L in AAA34437 (PubMed:2123815).Curated1
    Sequence conflicti329 – 332SYFT → FLLH in AAA34437 (PubMed:2123815).Curated4
    Sequence conflicti329 – 332SYFT → FLLH in CAA62528 (PubMed:8789261).Curated4
    Sequence conflicti329 – 332SYFT → FLLH in CAA99067 (PubMed:9169874).Curated4

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M35237 Genomic DNA Translation: AAA34437.1
    X91067 Genomic DNA Translation: CAA62528.1
    Z74800 Genomic DNA Translation: CAA99067.1
    X07070 Genomic DNA Translation: CAA30106.1
    BK006948 Genomic DNA Translation: DAA10725.2
    PIRiS59291 AJBYRS
    RefSeqiNP_014583.2, NM_001183313.2

    Genome annotation databases

    EnsemblFungiiYOL058W; YOL058W; YOL058W
    GeneIDi854096
    KEGGisce:YOL058W

    Similar proteinsi

    Entry informationi

    Entry nameiASSY_YEAST
    AccessioniPrimary (citable) accession number: P22768
    Secondary accession number(s): D6W209
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: September 21, 2011
    Last modified: May 23, 2018
    This is version 157 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Protein nomenclature information
      List of publications dealing with protein nomenclature and links to specialized websites

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