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Reviewed, UniProtKB/Swiss-Prot P22760 (AAAD_HUMAN)

Last modified June 16, 2009. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Arylacetamide deacetylase
      Short name=AADAC
    EC=3.1.1.-
Gene names
Name: AADAC
Synonyms: DAC
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length399 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Arylacetamide deacetylation is an important enzyme activity in the metabolic activation of arylamine substrates to ultimate carcinogens.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Microsome membrane; Single-pass type II membrane protein By similarity.

Tissue specificity

Liver.

Sequence similarities

Belongs to the 'GDXG' lipolytic enzyme family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   Coding sequence diversityPolymorphism
   DomainSignal-anchor
Transmembrane
   Molecular functionHydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processmetabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentendoplasmic reticulum membrane

Inferred from direct assay. Source: UniProtKB

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

microsome Ref.1

Traceable author statement. Source: ProtInc

   Molecular functioncarboxylesterase activity

Inferred from electronic annotation. Source: InterPro

deacetylase activity

Inferred from direct assay. Source: UniProtKB

lipase activity

Traceable author statement. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 399398Arylacetamide deacetylase
PRO_0000071542

Regions

Topological domain2 – 54Cytoplasmic Potential
Transmembrane6 – 2318Signal-anchor for type II membrane protein Potential
Topological domain24 – 399376Lumenal Potential

Sites

Active site1111 Potential
Active site1891 Potential

Amino acid modifications

Glycosylation781N-linked (GlcNAc...)
Glycosylation2821N-linked (GlcNAc...)
Disulfide bond116 ↔ 340 By similarity

Natural variations

Natural variant2811I → V: dbSNP rs1803155. Ref.2 Ref.5
VAR_014798

Experimental info

Sequence conflict31R → M AA sequence Ref.3
Sequence conflict53 – 575LGLHH → HGSSI in AAA35551. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P22760-1 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 6BF9CB7162D74966

FASTA39945,748
        10         20         30         40         50         60 
MGRKSLYLLI VGILIAYYIY TPLPDNVEEP WRMMWINAHL KTIQNLATFV ELLGLHHFMD 

        70         80         90        100        110        120 
SFKVVGSFDE VPPTSDENVT VTETKFNNIL VRVYVPKRKS EALRRGLFYI HGGGWCVGSA 

       130        140        150        160        170        180 
ALSGYDLLSR WTADRLDAVV VSTNYRLAPK YHFPIQFEDV YNALRWFLRK KVLAKYGVNP 

       190        200        210        220        230        240 
ERIGISGDSA GGNLAAAVTQ QLLDDPDVKI KLKIQSLIYP ALQPLDVDLP SYQENSNFLF 

       250        260        270        280        290        300 
LSKSLMVRFW SEYFTTDRSL EKAMLSRQHV PVESSHLFKF INWSSLLPER FIKGHVYNNP 

       310        320        330        340        350        360 
NYGSSELAKK YPGFLDVRAA PLLADDNKLR GLPLTYVITC QYDLLRDDGL MYVTRLRNTG 

       370        380        390 
VQVTHNHVED GFHGAFSFLG LKISHRLINQ YIEWLKENL 

« Hide

References

« Hide 'large scale' references
[1]"Human liver arylacetamide deacetylase. Molecular cloning of a novel esterase involved in the metabolic activation of arylamine carcinogens with high sequence similarity to hormone-sensitive lipase."
Probst M.R., Beer M., Beer D., Jenoe P., Meyer U.A., Gasser R.
J. Biol. Chem. 269:21650-21656(1994) [PubMed: 8063807] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-281.
Tissue: Colon.
[3]"Purification and characterization of a human liver arylacetamide deacetylase."
Probst M.R., Jenoe P., Meyer U.A.
Biochem. Biophys. Res. Commun. 177:453-459(1991) [PubMed: 2043131] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
Tissue: Liver.
[4]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-78 AND ASN-282, MASS SPECTROMETRY.
Tissue: Liver.
[5]"Catalog of 680 variations among eight cytochrome p450 (CYP) genes, nine esterase genes, and two other genes in the Japanese population."
Saito S., Iida A., Sekine A., Kawauchi S., Higuchi S., Ogawa C., Nakamura Y.
J. Hum. Genet. 48:249-270(2003) [PubMed: 12721789] [Abstract]
Cited for: VARIANT VAL-281.
+Additional computationally mapped references.

Cross-references

Sequence databases

L32179 mRNA. Translation: AAA35551.1.
BC032309 mRNA. Translation: AAH32309.1.
IPIIPI00383879.
PIRA53856.
RefSeqNP_001077.2.
UniGeneHs.506908

3D structure databases

HSSPHSSP built from PDB template 1JJI based on UniProtKB O28558.
ModBaseSearch...

Protein family/group databases

MEROPSS09.991.

PTM databases

PhosphoSiteP22760.

Proteomic databases

PRIDEP22760.

Genome annotation databases

EnsemblENSG00000114771. Homo sapiens. [Contig view]
GeneID13.
KEGGhsa:13.

Organism-specific databases

GeneCardsGC03P153014.
HGNCHGNC:17. AADAC.
HPAHPA002911.
MIM600338. gene.
PharmGKBPA24363.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP22760.
HOVERGENP22760.

Gene expression databases

ArrayExpressP22760.
BgeeP22760.
CleanExHS_AADAC.
GermOnlineENSG00000114771. Homo sapiens.

Family and domain databases

InterProIPR013094. AB_hydrolase_3.
IPR017157. Arylacetamide_deacetylase.
IPR002168. Lipase_GDXG_AS.
[Graphical view]
PfamPF07859. Abhydrolase_3. 1 hit.
[Graphical view]
PIRSFPIRSF037251. Arylacetamide_deacetylase. 1 hit.
PROSITEPS01173. LIPASE_GDXG_HIS. False negative.
PS01174. LIPASE_GDXG_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio27.
SOURCESearch...

Entry information

Entry nameAAAD_HUMAN
AccessionPrimary (citable) accession number: P22760
Secondary accession number(s): Q8N1A9
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 90 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents