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P22759

- BFR_AZOVI

UniProt

P22759 - BFR_AZOVI

Protein

Bacterioferritin

Gene

bfr

Organism
Azotobacter vinelandii
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Iron-storage protein, whose ferroxidase center binds Fe2+ ions, oxidizes them by dioxygen to Fe3+, and participates in the subsequent Fe3+ oxide mineral core formation within the central cavity of the protein complex.By similarity

    Catalytic activityi

    4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.

    Cofactori

    Binds 1 heme B (iron-protoporphyrin IX) group per dimer.By similarity
    Binds 2 iron ions per subunit. The catalytic dinuclear iron-binding site within each subunit is known as the ferroxidase center By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi18 – 181Iron 1PROSITE-ProRule annotation
    Metal bindingi51 – 511Iron 1PROSITE-ProRule annotation
    Metal bindingi51 – 511Iron 2PROSITE-ProRule annotation
    Metal bindingi52 – 521Iron (heme axial ligand); shared with dimeric partnerPROSITE-ProRule annotation
    Metal bindingi54 – 541Iron 1PROSITE-ProRule annotation
    Metal bindingi94 – 941Iron 2PROSITE-ProRule annotation
    Metal bindingi127 – 1271Iron 1PROSITE-ProRule annotation
    Metal bindingi127 – 1271Iron 2PROSITE-ProRule annotation
    Metal bindingi130 – 1301Iron 2PROSITE-ProRule annotation

    GO - Molecular functioni

    1. ferric iron binding Source: InterPro
    2. ferroxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular iron ion homeostasis Source: UniProtKB-KW
    2. iron ion transport Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Iron storage

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bacterioferritin (EC:1.16.3.1)
    Short name:
    BFR
    Alternative name(s):
    Cytochrome b-557.5
    Gene namesi
    Name:bfr
    OrganismiAzotobacter vinelandii
    Taxonomic identifieri354 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 156156BacterioferritinPRO_0000192588Add
    BLAST

    Interactioni

    Subunit structurei

    Homooligomer of 24 subunits, arranged as 12 dimers, that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be deposited.By similarity

    Structurei

    Secondary structure

    1
    156
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 3430
    Helixi38 – 6427
    Helixi83 – 11028
    Helixi114 – 14431
    Helixi146 – 1516

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SOFX-ray2.60A/B/C/D/E/F/G/H1-156[»]
    2FKZX-ray2.00A/B/C/D/E/F/G/H1-155[»]
    2FL0X-ray2.70A/B/C/D/E/F/G/H1-155[»]
    ProteinModelPortaliP22759.
    SMRiP22759. Positions 1-155.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP22759.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 145145Ferritin-like diironPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the bacterioferritin family.Curated
    Contains 1 ferritin-like diiron domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di1.20.1260.10. 1 hit.
    InterProiIPR002024. Bacterioferritin.
    IPR009040. Ferritin-like_diiron.
    IPR009078. Ferritin-like_SF.
    IPR012347. Ferritin-rel.
    IPR008331. Ferritin_DPS_dom.
    [Graphical view]
    PfamiPF00210. Ferritin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002560. Bacterioferritin. 1 hit.
    PRINTSiPR00601. BACFERRITIN.
    SUPFAMiSSF47240. SSF47240. 1 hit.
    TIGRFAMsiTIGR00754. bfr. 1 hit.
    PROSITEiPS00549. BACTERIOFERRITIN. 1 hit.
    PS50905. FERRITIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P22759-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKGDKIVIQH LNKILGNELI AINQYFLHAR MYEDWGLEKL GKHEYHESID    50
    EMKHADKLIK RILFLEGLPN LQELGKLLIG EHTKEMLECD LKLEQAGLPD 100
    LKAAIAYCES VGDYASRELL EDILESEEDH IDWLETQLDL IDKIGLENYL 150
    QSQMDE 156
    Length:156
    Mass (Da):18,105
    Last modified:May 1, 1992 - v2
    Checksum:i468C2F059240A647
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M83692 Genomic DNA. Translation: AAA22121.1.
    PIRiA41983.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M83692 Genomic DNA. Translation: AAA22121.1 .
    PIRi A41983.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SOF X-ray 2.60 A/B/C/D/E/F/G/H 1-156 [» ]
    2FKZ X-ray 2.00 A/B/C/D/E/F/G/H 1-155 [» ]
    2FL0 X-ray 2.70 A/B/C/D/E/F/G/H 1-155 [» ]
    ProteinModelPortali P22759.
    SMRi P22759. Positions 1-155.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P22759.

    Family and domain databases

    Gene3Di 1.20.1260.10. 1 hit.
    InterProi IPR002024. Bacterioferritin.
    IPR009040. Ferritin-like_diiron.
    IPR009078. Ferritin-like_SF.
    IPR012347. Ferritin-rel.
    IPR008331. Ferritin_DPS_dom.
    [Graphical view ]
    Pfami PF00210. Ferritin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002560. Bacterioferritin. 1 hit.
    PRINTSi PR00601. BACFERRITIN.
    SUPFAMi SSF47240. SSF47240. 1 hit.
    TIGRFAMsi TIGR00754. bfr. 1 hit.
    PROSITEi PS00549. BACTERIOFERRITIN. 1 hit.
    PS50905. FERRITIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Physical, chemical and immunological properties of the bacterioferritins of Escherichia coli, Pseudomonas aeruginosa and Azotobacter vinelandii."
      Andrews S.C., Findlay J.B.C., Guest J.R., Harrison P.M., Keen J.N., Smith J.M.A.
      Biochim. Biophys. Acta 1078:111-116(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-70.

    Entry informationi

    Entry nameiBFR_AZOVI
    AccessioniPrimary (citable) accession number: P22759
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3