Reviewed, UniProtKB/Swiss-Prot P22758 (TGM1_RABIT)

Names and origin

Protein names

Recommended name:
    Protein-glutamine gamma-glutamyltransferase K
    EC=2.3.2.13
Alternative name(s):
    Transglutaminase K
      Short name=TGase K
      Short name=TGK
      Short name=TG(K)
    Transglutaminase-1
    Epidermal TGase

Gene names

Name:

TGM1

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	836 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross-linking epidermal proteins during formation of the stratum corneum.
Catalytic activity	Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Enzyme regulation	Inhibited by retinoic acid, but phorbol ester treatment activates it.
Subcellular location	Membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the transglutaminase superfamily. Transglutaminase family.

Ontologies

Keywords
Biological process	Keratinization
Cellular component	Membrane
Ligand	Calcium Metal-binding
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	keratinization Inferred from electronic annotation. Source: UniProtKB-KW peptide cross-linking Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein-glutamine gamma-glutamyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 836	836	Protein-glutamine gamma-glutamyltransferase K		PRO_0000213703
Sites
	Active site	397	1	By similarity
	Active site	456	1	By similarity
	Active site	479	1	By similarity
	Metal binding	519	1	Calcium By similarity
	Metal binding	521	1	Calcium By similarity
	Metal binding	568	1	Calcium By similarity
	Metal binding	573	1	Calcium By similarity
Amino acid modifications
	Modified residue	98	1	Phosphoserine By similarity
	Modified residue	112	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22758-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: B3D281F7BECB9C00	FASTA	836	91,935
	10 20 30 40 50 60 MDGPRSDMGR SDVSRSDMSR SDMGRSDMGR SDVGRCGGGP LQPSATPSPE PEPEPEPEPD 70 80 90 100 110 120 RGSRSRGGRG RSFWARCCGC CSCRNAADDD WGREPSDSRD RGSSSRGGRP DSRGGGVNAA 130 140 150 160 170 180 GDGTIREGML VVTGVDLLSS RSDQNRREHH TDEFEYEELI VRRGQPFHLV LFLSRPYESS 190 200 210 220 230 240 DRIALELQIG NNPEVGKGTH VIIPVGKGNS GGWKAQVTKA SGQTLNLRVH SPASAIIGKF 250 260 270 280 290 300 QFTVRTRTEA GEFQLPFDPR NEIYILFNPW CPEDIVYVDH EDWRQDYVLN ESGRIYYGTE 310 320 330 340 350 360 AQIGERTWNY GQFDHGVLDA CLYILDRRGM PYGGRGDPVS VSRVISAMVN SLDDNGVLIG 370 380 390 400 410 420 NWSGDYSRGT NPSAWVGSVE ILLSYLRTGY SVPYGQCWVF AGVTTTVLRC LGLATRTVTN 430 440 450 460 470 480 FNSAHDTDTS LTMDIYFDEN MKPLEHLNRD SVWNFHVWND CWMKRPDLPS GFDGWQVVDA 490 500 510 520 530 540 TPQETSSGIF CCGPCSVESV KNGLVYMKYD TPFIFAEVNS DKVYWQRQDD GSFKIVYVEE 550 560 570 580 590 600 KAIGTLIVTK AVRSHMREDI THIYKHPEGS DAERKAVETA AAHGSKPNVY DSRDSAEDVA 610 620 630 640 650 660 MQVEAQDAVM GQDLTVSVVL TNRSSSRRTV KLHLYLSVTF YTGVTGSIFK ESKKEVVLAA 670 680 690 700 710 720 GSSDSVVMPV AYKEYRPHLV DQGAMLLNVS GHVKESGQVL AKQHTFRVRT PDLSLTLLGA 730 740 750 760 770 780 AVVGQECEVQ IVFRNPLPIT LTNVVFRLEG SGLQRPKILN VGDIGGNETV TLRQTFVPVR 790 800 810 820 830 PGPRQLIASL DSPQLSQVHG VIQVDVAPAS GGRGFLHAGG DSYSGETIPM TSRGEA

References

[1]	"Regulation of transglutaminase type I expression in squamous differentiating rabbit tracheal epithelial cells and human epidermal keratinocytes: effects of retinoic acid and phorbol esters." Saunders N.A., Bernacki S.H., Vollberg T.M., Jetten A.M. Mol. Endocrinol. 7:387-398(1993) [PubMed: 8097865] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: New Zealand white. Tissue: Tracheobronchial epithelium.
[2]	"Regulation of type I (epidermal) transglutaminase mRNA levels during squamous differentiation: down regulation by retinoids." Floyd E.E., Jetten A.M. Mol. Cell. Biol. 9:4846-4851(1989) [PubMed: 2574824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 368-749.
[3]	"The complete amino acid sequence of the human transglutaminase K enzyme deduced from the nucleic acid sequences of cDNA clones." Kim H.C., Idler W.W., Kim I.-G., Han J.H., Chung S.-I., Steinert P.M. J. Biol. Chem. 266:536-539(1991) [PubMed: 1670769] [Abstract] Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L10714 mRNA. No translation available. M30468 mRNA. Translation: AAA31475.1.
PIR	A33477. A54269.
UniGene	Ocu.1972
3D structure databases
HSSP	HSSP built from PDB template 1KV3 based on UniProtKB P21980.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P22758.
Enzyme and pathway databases
BRENDA	2.3.2.13. 255.
Family and domain databases
InterPro	IPR008957. Fibronectin_typ-III-like_fold. IPR013783. Ig-like_fold. IPR002931. Transglutaminase-like. IPR008958. Transglutaminase_C. IPR013808. Transglutaminase_CS. IPR001102. Transglutaminase_N. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00927. Transglut_C. 2 hits. PF01841. Transglut_core. 1 hit. PF00868. Transglut_N. 1 hit. [Graphical view]
SMART	SM00460. TGc. 1 hit. [Graphical view]
PROSITE	PS00547. TRANSGLUTAMINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TGM1_RABIT

Accession

Primary (citable) accession number: P22758

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)