Reviewed, UniProtKB/Swiss-Prot P22757 (HE_PARLI)

Names and origin

Protein names	Recommended name:     Hatching enzyme       Short name=HEZ       Short name=HE     EC=3.4.24.12 Alternative name(s):     Envelysin     Sea-urchin-hatching proteinase Cleaved into the following chain:     1- Recommended name:             Hatching enzyme 18 kDa form
Organism	Paracentrotus lividus (Common sea urchin)
Taxonomic identifier	7656 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Echinodermata › Eleutherozoa › Echinozoa › Echinoidea › Euechinoidea › Echinacea › Echinoida › Echinidae › Paracentrotus

Protein attributes

Sequence length	587 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Allows the sea urchin to digest the protective envelope derived from the egg extracellular matrix; thus allowing the sea urchin to swim freely.
Catalytic activity	Hydrolysis of proteins of the fertilization envelope and dimethylcasein.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Developmental stage	Embryo, blastula stage.
Domain	There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity. The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Sequence similarities	Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.

Ontologies

Keywords
Domain	Repeat Signal
Ligand	Calcium Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Autocatalytic cleavage Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: InterPro
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Probable
	Propeptide	19 – 166	148	Activation peptide Ref.1		PRO_0000028864
	Chain	167 – 587	421	Hatching enzyme		PRO_0000028865
	Chain	167 – 324	158	Hatching enzyme 18 kDa form		PRO_0000028866
Regions
	Domain	384 – 426	43	Hemopexin-like 1
	Domain	428 – 470	43	Hemopexin-like 2
	Domain	472 – 515	44	Hemopexin-like 3
	Domain	521 – 565	45	Hemopexin-like 4
	Motif	157 – 164	8	Cysteine switch By similarity
	Compositional bias	334 – 376	43	Arg/Thr-rich (hinge region)
Sites
	Active site	284	1	By similarity
	Metal binding	159	1	Zinc; in inhibited form By similarity
	Metal binding	283	1	Zinc; catalytic By similarity
	Metal binding	287	1	Zinc; catalytic By similarity
	Metal binding	293	1	Zinc; catalytic By similarity
	Site	324 – 325	2	Cleavage; by autolysis
Amino acid modifications
	Glycosylation	64	1	N-linked (GlcNAc...) Potential
	Glycosylation	126	1	N-linked (GlcNAc...) Potential
	Glycosylation	141	1	N-linked (GlcNAc...) Potential
	Glycosylation	584	1	N-linked (GlcNAc...) Potential
	Disulfide bond	380 ↔ 582		By similarity
Experimental info
	Sequence conflict	158	1	R → L in CAA46638. Ref.2
	Sequence conflict	196	1	V → A in CAA46638. Ref.2
	Sequence conflict	269	1	E → I in CAA46638. Ref.2
	Sequence conflict	316	1	N → S in CAA46638. Ref.2
	Sequence conflict	558	1	P → A in CAA46638. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P22757-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 0A1733EC547279AA	FASTA	587	65,207
	10 20 30 40 50 60 MANSGLILLV MFMIHVTTVH NVPLPSTAPS IITQLSDITT SIIEEDAFGL TTPTTGLLTP 70 80 90 100 110 120 VSENDSDDDG DDITTIQTTT SSSQTVISGV VVEEGVHESN VEILKAHLEK FGYTPPGSTF 130 140 150 160 170 180 GEANLNYTSA ILDFQEHGGI NQTGILDADT AELLSTPRCG VPDVLPFVTS SITWSRNQPV 190 200 210 220 230 240 TYSFGALTSD LNQNDVKDEI RRAFRVWDDV SGLSFREVPD TTSVDIRIKF GSYDHGDGIS 250 260 270 280 290 300 FDGRGGVLAH AFLPRNGDAH FDDSETWTEG TRSGTNLFQV AAHEFGHSLG LYHSTVRSAL 310 320 330 340 350 360 MYPYYQGYVP NFRLDNDDIA GIRSLYGSNS GSGTTTTTRR PTTTRATTTR RTTTTRATTT 370 380 390 400 410 420 RATTTTTTSP SRPSPPRRAC SGSFDAVVRD SSNRIYALTG PYFWQLDQPS PSWGLVSNRF 430 440 450 460 470 480 GFGLPQNIDA SFQRGVVTYF FSECYYYYQT STQRNFPRIP VNRKWVGLPC NIDAVYRSSR 490 500 510 520 530 540 GPTYFFKDSF VYKFNSNNRL QRRTRISSLF NDVPSALHDG VEAVVRADRN YIHFYRDGRY 550 560 570 580 YRMTDYGRQF VNFPNGLPYS DVIESVIPQC RGRSLSYESE GCSNSSE

References

[1]	"Early expression of a collagenase-like hatching enzyme gene in the sea urchin embryo." Lepage T., Gache C. EMBO J. 9:3003-3012(1990) [PubMed: 2167841] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 167-191 AND 325-333.
[2]	"Structure of the sea urchin hatching enzyme gene." Ghiglione C., Lhomond G., Lepage T., Gache C. Eur. J. Biochem. 219:845-854(1994) [PubMed: 8112336] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X53598 mRNA. Translation: CAA37667.1. X65722 Genomic DNA. Translation: CAA46638.1.
PIR	S12805. S41409.
3D structure databases
HSSP	HSSP built from PDB template 1HY7 based on UniProtKB P08254.
ModBase	Search...
Protein family/group databases
MEROPS	M10.010.
Enzyme and pathway databases
BRENDA	3.4.24.12. 39472.
Family and domain databases
InterPro	IPR000585. Hemopexin/matrixin. IPR018486. Hemopexin/matrixin_CS. IPR018487. Hemopexin/matrixin_repeat. IPR001818. Pept_M10A_M12B. IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR002477. Peptidoglycan-bd-like. [Graphical view]
Gene3D	G3DSA:2.110.10.10. Hemopexin. 1 hit.
Pfam	PF00045. Hemopexin. 4 hits. PF00413. Peptidase_M10. 1 hit. PF01471. PG_binding_1. 1 hit. [Graphical view]
PRINTS	PR00138. MATRIXIN.
SMART	SM00120. HX. 4 hits. SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00546. CYSTEINE_SWITCH. 1 hit. PS00024. HEMOPEXIN. False negative. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
PMAP-CutDB	P22757.

Entry information

Entry name

HE_PARLI

Accession

Primary (citable) accession number: P22757

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)