P22751 (PLYX_ERWCH) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 52.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Pectate disaccharide-lyase EC=4.2.2.9 Alternative name(s): Exopolygalacturonate lyase Short name=ExoPL | ||
| Gene names |
| ||
| Organism | Erwinia chrysanthemi | ||
| Taxonomic identifier | 556 [NCBI] | ||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya |
Protein attributes
| Sequence length | 749 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | Eliminative cleavage of 4-(4-deoxy-alpha-D-galact-4-enuronosyl)-D-galacturonate from the reducing end of pectate, i.e. de-esterified pectin. |
| Cofactor | Calcium. |
| Sequence similarities | Belongs to the polysaccharide lyase 9 family. |
Ontologies
| Keywords | |
|---|---|
| Domain | Signal |
| Ligand | Calcium Metal-binding |
| Molecular function | Lyase |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Molecular function | metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate disaccharide-lyase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 26 | 26 | Ref.1 | ||||||
| Chain | 27 – 749 | 723 | Pectate disaccharide-lyase | PRO_0000024935 | |||||
Sites | |||||||||
| Active site | 595 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 538 | 1 | Calcium By similarity | ||||||
| Metal binding | 562 | 1 | Calcium By similarity | ||||||
| Metal binding | 563 | 1 | Calcium By similarity | ||||||
| Metal binding | 566 | 1 | Calcium By similarity | ||||||
Sequences
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References
| [1] | "Molecular cloning of the structural gene for exopolygalacturonate lyase from Erwinia chrysanthemi EC16 and characterization of the enzyme product." Brooks A.D., Yang He S., Gold S., Keen N.T., Collmer A., Hutcheson S.W. J. Bacteriol. 172:6950-6958(1990) [PubMed: 2254266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 27-46. Strain: EC16. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M62739 Genomic DNA. Translation: AAA24850.1. |
| PIR | A37839. |
3D structure databases | |
| ProteinModelPortal | P22751. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | PL9. Polysaccharide Lyase Family 9. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Family and domain databases | |
| InterPro | IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view] |
| Gene3D | G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit. |
| SMART | SM00710. PbH1. 4 hits. [Graphical view] |
| SUPFAM | SSF51126. Pectin_lyas_like. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | PLYX_ERWCH | ||||||||
| Accession | Primary (citable) accession number: P22751 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

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