Reviewed, UniProtKB/Swiss-Prot P22751 (PELX_ERWCH)

Names and origin

Protein names

Recommended name:
    Pectate disaccharide-lyase
    EC=4.2.2.9
Alternative name(s):
    Exopolygalacturonate lyase
      Short name=ExoPL

Gene names

Name:

pelX

Organism

Erwinia chrysanthemi

Taxonomic identifier

556 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Dickeya

Protein attributes

Sequence length	749 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Eliminative cleavage of 4-(4-deoxy-alpha-D-galact-4-enuronosyl)-D-galacturonate from the reducing end of pectate, i.e. de-esterified pectin.
Cofactor	Calcium.
Sequence similarities	Belongs to the polysaccharide lyase 9 family.

Ontologies

Keywords
Domain	Signal
Ligand	Calcium
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pectate disaccharide-lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Ref.1
	Chain	27 – 749	723	Pectate disaccharide-lyase		PRO_0000024935

Sequences

	Sequence		Length	Mass (Da)
	P22751-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 449BB25C23F2A9B5	FASTA	749	82,240
	10 20 30 40 50 60 MKYAASGLLS VALNSLLLLG SNQRFATQDV APVWRGIAFG QSTDVNFATN VLPEKVGVND 70 80 90 100 110 120 VTINGKKLTV NDKADLSAPI TIESRGGKIA NTHDGLTFFY TQLPANVNFT LQSDVTVEQF 130 140 150 160 170 180 GPESDAKPNA QEGAGLLVRD ILGVPRQEPL KEGYEEFPAA SNMVMNAIMT QDKKSKTEVK 190 200 210 220 230 240 MQLISRNGVT QPWGNTNAEI TRTSYQEKIN LEQTPTFRLK LERTNDGFIT AYAPKGSDQW 250 260 270 280 290 300 VSKTVKGADL VTHQDKDHYY VGFFASRNAK ITISNASLTT SPANTKPSAP FKAETTAPLL 310 320 330 340 350 360 QVASSSLSTS DTYPVQARVN YNGTVEVFQN GKSLGKPQRV RAGDDFSLTT RLTQQKSDFK 370 380 390 400 410 420 LVYIPSEGED KTAKETSFSV EKITLADARN LYVSPEGKAG NDGSKNAPLD IKTAINALPG 430 440 450 460 470 480 GGTLWLMDGD YSATVIPVSA TQRKGMKTLM PVGKKAVFHG LQLNASYWKV KGIEITEKSF 490 500 510 520 530 540 RIEGSHNQIE RLLAHHCDNT GIQVSSSDNV GRPLWASHNL ILNSESHSNQ HPSKKDADGF 550 560 570 580 590 600 AVKMRVGEGN VIRGAFSHDN VDDGFDLFNK IEDGPNGAVM IENSISLNNT SNGFKLGGEG 610 620 630 640 650 660 QPVAHQVKNS IAIGNHMDGF SDNFNPGALQ VSNNIALDNV RFNFIFRPSP YYGYEKQGIF 670 680 690 700 710 720 KNNVSLRTQP GKYDDAVVGR LDASNYFIRI IERSTVRVRK SRRRITNPSR CQRSSAGMKK 730 740 AACNWVIFCR RSNRHKTQRH RNRYPSTPA

References

[1]

"Molecular cloning of the structural gene for exopolygalacturonate lyase from Erwinia chrysanthemi EC16 and characterization of the enzyme product." Brooks A.D., Yang He S., Gold S., Keen N.T., Collmer A., Hutcheson S.W. J. Bacteriol. 172:6950-6958(1990) [PubMed: 2254266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 27-46. Strain: EC16.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M62739 Genomic DNA. Translation: AAA24850.1.
PIR	A37839.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL9. Polysaccharide Lyase Family 9.
Enzyme and pathway databases
BRENDA	4.2.2.9. 1459.
Family and domain databases
InterPro	IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
SMART	SM00710. PbH1. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PELX_ERWCH

Accession

Primary (citable) accession number: P22751

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	April 14, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)