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Protein

Granulysin

Gene

GNLY

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Fungicide

Protein family/group databases

TCDBi1.C.35.3.2. the amoebapore (amoebapore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Granulysin
Alternative name(s):
Lymphokine LAG-2
Protein NKG5
T-cell activation protein 519
Gene namesi
Name:GNLY
Synonyms:LAG2, NKG5, TLA519
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:4414. GNLY.

Subcellular locationi

  • Secreted

  • Note: Located in the cytotoxic granules of T-cells, which are released upon antigen stimulation.

GO - Cellular componenti

  • extracellular space Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28793.

Polymorphism and mutation databases

BioMutaiGNLY.
DMDMi116242500.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 145123GranulysinPRO_0000031659Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi69 ↔ 132
Disulfide bondi96 ↔ 107

Post-translational modificationi

A 9 kDa form is produced by proteolytic processing of a 15 kDa protein.1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP22749.
PRIDEiP22749.

PTM databases

PhosphoSiteiP22749.

Expressioni

Tissue specificityi

Expressed in natural killer and T-cells.

Inductioni

By T-cell growth factor and IL2/interleukin-2.

Gene expression databases

BgeeiP22749.
CleanExiHS_GNLY.
ExpressionAtlasiP22749. baseline and differential.
GenevisibleiP22749. HS.

Organism-specific databases

HPAiCAB025186.
HPA058021.

Interactioni

Protein-protein interaction databases

BioGridi115829. 1 interaction.
STRINGi9606.ENSP00000263863.

Structurei

Secondary structure

1
145
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi64 – 7916Combined sources
Helixi85 – 9410Combined sources
Helixi95 – 973Combined sources
Helixi101 – 12323Combined sources
Helixi128 – 1325Combined sources
Helixi133 – 1353Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1L9LX-ray0.92A63-136[»]
ProteinModelPortaliP22749.
SMRiP22749. Positions 63-136.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22749.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini62 – 14281Saposin B-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 saposin B-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG68808.
GeneTreeiENSGT00390000002975.
HOGENOMiHOG000112748.
HOVERGENiHBG005866.
InParanoidiP22749.
PhylomeDBiP22749.
TreeFamiTF342210.

Family and domain databases

Gene3Di1.10.225.10. 1 hit.
InterProiIPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB.
[Graphical view]
PfamiPF03489. SapB_2. 1 hit.
[Graphical view]
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
PROSITEiPS50015. SAP_B. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P22749-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATWALLLLA AMLLGNPGLV FSRLSPEYYD LARAHLRDEE KSCPCLAQEG
60 70 80 90 100
PQGDLLTKTQ ELGRDYRTCL TIVQKLKKMV DKPTQRSVSN AATRVCRTGR
110 120 130 140
SRWRDVCRNF MRRYQSRVTQ GLVAGETAQQ ICEDLRLCIP STGPL
Length:145
Mass (Da):16,374
Last modified:October 17, 2006 - v3
Checksum:iCEC5432FFBDBA1F4
GO
Isoform Short (identifier: P22749-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: MATWALLLLAAMLLGNP → ME

Show »
Length:130
Mass (Da):14,853
Checksum:i6F5238713B905CF8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311L → P in CAA28715 (PubMed:2434598).Curated
Sequence conflicti39 – 391E → G in CAA28715 (PubMed:2434598).Curated
Sequence conflicti46 – 472LA → G in CAA28715 (PubMed:2434598).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti119 – 1191T → I.2 Publications
Corresponds to variant rs11127 [ dbSNP | Ensembl ].
VAR_027868

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1717MATWA…LLGNP → ME in isoform Short. 1 PublicationVSP_006016Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05044 mRNA. Translation: CAA28715.1.
X54101 mRNA. Translation: CAA38035.1.
M85276 Genomic DNA. Translation: AAA59935.1.
CR541859 mRNA. Translation: CAG46657.1.
BC023576 mRNA. Translation: AAH23576.1.
CCDSiCCDS1984.1. [P22749-1]
CCDS46354.1. [P22749-2]
PIRiA27562.
I54504.
RefSeqiNP_001289687.1. NM_001302758.1.
NP_006424.2. NM_006433.4. [P22749-1]
NP_036615.2. NM_012483.3. [P22749-2]
UniGeneiHs.105806.

Genome annotation databases

EnsembliENST00000263863; ENSP00000263863; ENSG00000115523.
ENST00000409696; ENSP00000387116; ENSG00000115523. [P22749-2]
GeneIDi10578.
KEGGihsa:10578.
UCSCiuc002sql.4. human. [P22749-1]
uc010fgp.3. human. [P22749-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05044 mRNA. Translation: CAA28715.1.
X54101 mRNA. Translation: CAA38035.1.
M85276 Genomic DNA. Translation: AAA59935.1.
CR541859 mRNA. Translation: CAG46657.1.
BC023576 mRNA. Translation: AAH23576.1.
CCDSiCCDS1984.1. [P22749-1]
CCDS46354.1. [P22749-2]
PIRiA27562.
I54504.
RefSeqiNP_001289687.1. NM_001302758.1.
NP_006424.2. NM_006433.4. [P22749-1]
NP_036615.2. NM_012483.3. [P22749-2]
UniGeneiHs.105806.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1L9LX-ray0.92A63-136[»]
ProteinModelPortaliP22749.
SMRiP22749. Positions 63-136.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115829. 1 interaction.
STRINGi9606.ENSP00000263863.

Protein family/group databases

TCDBi1.C.35.3.2. the amoebapore (amoebapore) family.

PTM databases

PhosphoSiteiP22749.

Polymorphism and mutation databases

BioMutaiGNLY.
DMDMi116242500.

Proteomic databases

PaxDbiP22749.
PRIDEiP22749.

Protocols and materials databases

DNASUi10578.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263863; ENSP00000263863; ENSG00000115523.
ENST00000409696; ENSP00000387116; ENSG00000115523. [P22749-2]
GeneIDi10578.
KEGGihsa:10578.
UCSCiuc002sql.4. human. [P22749-1]
uc010fgp.3. human. [P22749-2]

Organism-specific databases

CTDi10578.
GeneCardsiGC02P085912.
HGNCiHGNC:4414. GNLY.
HPAiCAB025186.
HPA058021.
MIMi188855. gene.
neXtProtiNX_P22749.
PharmGKBiPA28793.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG68808.
GeneTreeiENSGT00390000002975.
HOGENOMiHOG000112748.
HOVERGENiHBG005866.
InParanoidiP22749.
PhylomeDBiP22749.
TreeFamiTF342210.

Miscellaneous databases

ChiTaRSiGNLY. human.
EvolutionaryTraceiP22749.
GeneWikiiGNLY.
GenomeRNAii10578.
NextBioi40149.
PROiP22749.
SOURCEiSearch...

Gene expression databases

BgeeiP22749.
CleanExiHS_GNLY.
ExpressionAtlasiP22749. baseline and differential.
GenevisibleiP22749. HS.

Family and domain databases

Gene3Di1.10.225.10. 1 hit.
InterProiIPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB.
[Graphical view]
PfamiPF03489. SapB_2. 1 hit.
[Graphical view]
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
PROSITEiPS50015. SAP_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The isolation and sequence of a novel gene from a human functional T cell line."
    Jongstra J., Schall T.J., Dyer B.J., Clayberger C., Jorgensen J., Davis M.M., Krensky A.M.
    J. Exp. Med. 165:601-614(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT), VARIANT ILE-119.
  2. "A cDNA clone expressed in natural killer and T cells that likely encodes a secreted protein."
    Yabe T., McSherry C., Bach F.H., Houchins J.P.
    J. Exp. Med. 172:1159-1163(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
  3. "Genomic structure of NKG5, a human NK and T cell-specific activation gene."
    Houchins J.P., Kricek F., Chujor C.S., Heise C.P., Yabe T., McSherry C., Bach F.H.
    Immunogenetics 37:102-107(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ILE-119.
    Tissue: Placenta.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: B-cell.
  6. "Granulysin: a novel antimicrobial peptide of cytolytic T lymphocytes and natural killer cells."
    Krensky A.M.
    Biochem. Pharmacol. 59:317-320(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  7. Cited for: CHARACTERIZATION.
  8. "Biosynthesis of granulysin, a novel cytolytic molecule."
    Hanson D.A., Kaspar A.A., Poulain F.R., Krensky A.M.
    Mol. Immunol. 36:413-422(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING.
  9. "Granulysin crystal structure and a structure-derived lytic mechanism."
    Anderson D.H., Sawaya M.R., Cascio D., Ernst W., Modlin R., Krensky A., Eisenberg D.
    J. Mol. Biol. 325:355-365(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (0.92 ANGSTROMS) OF 63-136.

Entry informationi

Entry nameiGNLY_HUMAN
AccessioniPrimary (citable) accession number: P22749
Secondary accession number(s): P09325, Q6GU08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: October 17, 2006
Last modified: July 22, 2015
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.