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P22749

- GNLY_HUMAN

UniProt

P22749 - GNLY_HUMAN

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Protein
Granulysin
Gene
GNLY, LAG2, NKG5, TLA519
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.

GO - Biological processi

  1. cellular defense response Source: ProtInc
  2. defense response to bacterium Source: UniProtKB-KW
  3. defense response to fungus Source: UniProtKB-KW
  4. killing of cells of other organism Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Fungicide

Protein family/group databases

TCDBi1.C.35.3.2. the amoebapore (amoebapore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Granulysin
Alternative name(s):
Lymphokine LAG-2
Protein NKG5
T-cell activation protein 519
Gene namesi
Name:GNLY
Synonyms:LAG2, NKG5, TLA519
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:4414. GNLY.

Subcellular locationi

Secreted
Note: Located in the cytotoxic granules of T-cells, which are released upon antigen stimulation.

GO - Cellular componenti

  1. extracellular space Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28793.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed prediction
Add
BLAST
Chaini23 – 145123Granulysin
PRO_0000031659Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi69 ↔ 132
Disulfide bondi96 ↔ 107

Post-translational modificationi

A 9 kDa form is produced by proteolytic processing of a 15 kDa protein.

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP22749.
PRIDEiP22749.

PTM databases

PhosphoSiteiP22749.

Expressioni

Tissue specificityi

Expressed in natural killer and T-cells.

Inductioni

By T-cell growth factor and IL2/interleukin-2.

Gene expression databases

ArrayExpressiP22749.
BgeeiP22749.
CleanExiHS_GNLY.
GenevestigatoriP22749.

Organism-specific databases

HPAiCAB025186.

Interactioni

Protein-protein interaction databases

BioGridi115829. 1 interaction.
STRINGi9606.ENSP00000263863.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi64 – 7916
Helixi85 – 9410
Helixi95 – 973
Helixi101 – 12323
Helixi128 – 1325
Helixi133 – 1353

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1L9LX-ray0.92A63-136[»]
ProteinModelPortaliP22749.
SMRiP22749. Positions 63-136.

Miscellaneous databases

EvolutionaryTraceiP22749.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini62 – 14281Saposin B-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG68808.
HOGENOMiHOG000112748.
HOVERGENiHBG005866.
InParanoidiP22749.
PhylomeDBiP22749.
TreeFamiTF342210.

Family and domain databases

Gene3Di1.10.225.10. 1 hit.
InterProiIPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB.
[Graphical view]
PfamiPF03489. SapB_2. 1 hit.
[Graphical view]
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
PROSITEiPS50015. SAP_B. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P22749-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MATWALLLLA AMLLGNPGLV FSRLSPEYYD LARAHLRDEE KSCPCLAQEG    50
PQGDLLTKTQ ELGRDYRTCL TIVQKLKKMV DKPTQRSVSN AATRVCRTGR 100
SRWRDVCRNF MRRYQSRVTQ GLVAGETAQQ ICEDLRLCIP STGPL 145
Length:145
Mass (Da):16,374
Last modified:October 17, 2006 - v3
Checksum:iCEC5432FFBDBA1F4
GO
Isoform Short (identifier: P22749-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: MATWALLLLAAMLLGNP → ME

Show »
Length:130
Mass (Da):14,853
Checksum:i6F5238713B905CF8
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti119 – 1191T → I.2 Publications
Corresponds to variant rs11127 [ dbSNP | Ensembl ].
VAR_027868

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1717MATWA…LLGNP → ME in isoform Short.
VSP_006016Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311L → P in CAA28715. 1 Publication
Sequence conflicti39 – 391E → G in CAA28715. 1 Publication
Sequence conflicti46 – 472LA → G in CAA28715. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05044 mRNA. Translation: CAA28715.1.
X54101 mRNA. Translation: CAA38035.1.
M85276 Genomic DNA. Translation: AAA59935.1.
CR541859 mRNA. Translation: CAG46657.1.
BC023576 mRNA. Translation: AAH23576.1.
CCDSiCCDS1984.1. [P22749-1]
CCDS46354.1. [P22749-2]
PIRiA27562.
I54504.
RefSeqiNP_006424.2. NM_006433.3. [P22749-1]
NP_036615.2. NM_012483.2. [P22749-2]
UniGeneiHs.105806.

Genome annotation databases

EnsembliENST00000263863; ENSP00000263863; ENSG00000115523. [P22749-1]
ENST00000409696; ENSP00000387116; ENSG00000115523. [P22749-2]
GeneIDi10578.
KEGGihsa:10578.
UCSCiuc002sql.4. human. [P22749-1]
uc010fgp.3. human. [P22749-2]

Polymorphism databases

DMDMi116242500.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05044 mRNA. Translation: CAA28715.1 .
X54101 mRNA. Translation: CAA38035.1 .
M85276 Genomic DNA. Translation: AAA59935.1 .
CR541859 mRNA. Translation: CAG46657.1 .
BC023576 mRNA. Translation: AAH23576.1 .
CCDSi CCDS1984.1. [P22749-1 ]
CCDS46354.1. [P22749-2 ]
PIRi A27562.
I54504.
RefSeqi NP_006424.2. NM_006433.3. [P22749-1 ]
NP_036615.2. NM_012483.2. [P22749-2 ]
UniGenei Hs.105806.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1L9L X-ray 0.92 A 63-136 [» ]
ProteinModelPortali P22749.
SMRi P22749. Positions 63-136.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115829. 1 interaction.
STRINGi 9606.ENSP00000263863.

Protein family/group databases

TCDBi 1.C.35.3.2. the amoebapore (amoebapore) family.

PTM databases

PhosphoSitei P22749.

Polymorphism databases

DMDMi 116242500.

Proteomic databases

PaxDbi P22749.
PRIDEi P22749.

Protocols and materials databases

DNASUi 10578.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000263863 ; ENSP00000263863 ; ENSG00000115523 . [P22749-1 ]
ENST00000409696 ; ENSP00000387116 ; ENSG00000115523 . [P22749-2 ]
GeneIDi 10578.
KEGGi hsa:10578.
UCSCi uc002sql.4. human. [P22749-1 ]
uc010fgp.3. human. [P22749-2 ]

Organism-specific databases

CTDi 10578.
GeneCardsi GC02P085912.
HGNCi HGNC:4414. GNLY.
HPAi CAB025186.
MIMi 188855. gene.
neXtProti NX_P22749.
PharmGKBi PA28793.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG68808.
HOGENOMi HOG000112748.
HOVERGENi HBG005866.
InParanoidi P22749.
PhylomeDBi P22749.
TreeFami TF342210.

Miscellaneous databases

ChiTaRSi GNLY. human.
EvolutionaryTracei P22749.
GeneWikii GNLY.
GenomeRNAii 10578.
NextBioi 40149.
PROi P22749.
SOURCEi Search...

Gene expression databases

ArrayExpressi P22749.
Bgeei P22749.
CleanExi HS_GNLY.
Genevestigatori P22749.

Family and domain databases

Gene3Di 1.10.225.10. 1 hit.
InterProi IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB.
[Graphical view ]
Pfami PF03489. SapB_2. 1 hit.
[Graphical view ]
SMARTi SM00741. SapB. 1 hit.
[Graphical view ]
SUPFAMi SSF47862. SSF47862. 1 hit.
PROSITEi PS50015. SAP_B. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The isolation and sequence of a novel gene from a human functional T cell line."
    Jongstra J., Schall T.J., Dyer B.J., Clayberger C., Jorgensen J., Davis M.M., Krensky A.M.
    J. Exp. Med. 165:601-614(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT), VARIANT ILE-119.
  2. "A cDNA clone expressed in natural killer and T cells that likely encodes a secreted protein."
    Yabe T., McSherry C., Bach F.H., Houchins J.P.
    J. Exp. Med. 172:1159-1163(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
  3. "Genomic structure of NKG5, a human NK and T cell-specific activation gene."
    Houchins J.P., Kricek F., Chujor C.S., Heise C.P., Yabe T., McSherry C., Bach F.H.
    Immunogenetics 37:102-107(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ILE-119.
    Tissue: Placenta.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: B-cell.
  6. "Granulysin: a novel antimicrobial peptide of cytolytic T lymphocytes and natural killer cells."
    Krensky A.M.
    Biochem. Pharmacol. 59:317-320(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  7. Cited for: CHARACTERIZATION.
  8. "Biosynthesis of granulysin, a novel cytolytic molecule."
    Hanson D.A., Kaspar A.A., Poulain F.R., Krensky A.M.
    Mol. Immunol. 36:413-422(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING.
  9. "Granulysin crystal structure and a structure-derived lytic mechanism."
    Anderson D.H., Sawaya M.R., Cascio D., Ernst W., Modlin R., Krensky A., Eisenberg D.
    J. Mol. Biol. 325:355-365(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (0.92 ANGSTROMS) OF 63-136.

Entry informationi

Entry nameiGNLY_HUMAN
AccessioniPrimary (citable) accession number: P22749
Secondary accession number(s): P09325, Q6GU08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: October 17, 2006
Last modified: July 9, 2014
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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