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Protein

Granulysin

Gene

GNLY

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Fungicide

Enzyme and pathway databases

BioCyciZFISH:ENSG00000115523-MONOMER.
ReactomeiR-HSA-6803157. Antimicrobial peptides.

Protein family/group databases

TCDBi1.C.35.3.2. the amoebapore (amoebapore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Granulysin
Alternative name(s):
Lymphokine LAG-2
Protein NKG5
T-cell activation protein 519
Gene namesi
Name:GNLY
Synonyms:LAG2, NKG5, TLA519
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:4414. GNLY.

Subcellular locationi

  • Secreted

  • Note: Located in the cytotoxic granules of T-cells, which are released upon antigen stimulation.

GO - Cellular componenti

  • extracellular space Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi10578.
OpenTargetsiENSG00000115523.
PharmGKBiPA28793.

Polymorphism and mutation databases

BioMutaiGNLY.
DMDMi116242500.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000003165923 – 145GranulysinAdd BLAST123

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi69 ↔ 132
Disulfide bondi96 ↔ 107

Post-translational modificationi

A 9 kDa form is produced by proteolytic processing of a 15 kDa protein.1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP22749.
PeptideAtlasiP22749.
PRIDEiP22749.

PTM databases

iPTMnetiP22749.
PhosphoSitePlusiP22749.

Expressioni

Tissue specificityi

Expressed in natural killer and T-cells.

Inductioni

By T-cell growth factor and IL2/interleukin-2.

Gene expression databases

BgeeiENSG00000115523.
CleanExiHS_GNLY.
ExpressionAtlasiP22749. baseline and differential.
GenevisibleiP22749. HS.

Organism-specific databases

HPAiCAB025186.
HPA058021.

Interactioni

Protein-protein interaction databases

BioGridi115829. 1 interactor.
STRINGi9606.ENSP00000263863.

Structurei

Secondary structure

1145
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi64 – 79Combined sources16
Helixi85 – 94Combined sources10
Helixi95 – 97Combined sources3
Helixi101 – 123Combined sources23
Helixi128 – 132Combined sources5
Helixi133 – 135Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1L9LX-ray0.92A63-136[»]
ProteinModelPortaliP22749.
SMRiP22749.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22749.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini62 – 142Saposin B-typePROSITE-ProRule annotationAdd BLAST81

Sequence similaritiesi

Contains 1 saposin B-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J70I. Eukaryota.
ENOG41117YV. LUCA.
GeneTreeiENSGT00390000002975.
HOGENOMiHOG000112748.
HOVERGENiHBG005866.
InParanoidiP22749.
PhylomeDBiP22749.
TreeFamiTF342210.

Family and domain databases

Gene3Di1.10.225.10. 1 hit.
InterProiIPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PfamiPF03489. SapB_2. 1 hit.
[Graphical view]
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
PROSITEiPS50015. SAP_B. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P22749-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATWALLLLA AMLLGNPGLV FSRLSPEYYD LARAHLRDEE KSCPCLAQEG
60 70 80 90 100
PQGDLLTKTQ ELGRDYRTCL TIVQKLKKMV DKPTQRSVSN AATRVCRTGR
110 120 130 140
SRWRDVCRNF MRRYQSRVTQ GLVAGETAQQ ICEDLRLCIP STGPL
Length:145
Mass (Da):16,374
Last modified:October 17, 2006 - v3
Checksum:iCEC5432FFBDBA1F4
GO
Isoform Short (identifier: P22749-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-17: MATWALLLLAAMLLGNP → ME

Show »
Length:130
Mass (Da):14,853
Checksum:i6F5238713B905CF8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31L → P in CAA28715 (PubMed:2434598).Curated1
Sequence conflicti39E → G in CAA28715 (PubMed:2434598).Curated1
Sequence conflicti46 – 47LA → G in CAA28715 (PubMed:2434598).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_027868119T → I.2 PublicationsCorresponds to variant rs11127dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0060161 – 17MATWA…LLGNP → ME in isoform Short. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05044 mRNA. Translation: CAA28715.1.
X54101 mRNA. Translation: CAA38035.1.
M85276 Genomic DNA. Translation: AAA59935.1.
CR541859 mRNA. Translation: CAG46657.1.
BC023576 mRNA. Translation: AAH23576.1.
CCDSiCCDS1984.1. [P22749-1]
CCDS46354.1. [P22749-2]
PIRiA27562.
I54504.
RefSeqiNP_001289687.1. NM_001302758.1.
NP_006424.2. NM_006433.4. [P22749-1]
NP_036615.2. NM_012483.3. [P22749-2]
UniGeneiHs.105806.

Genome annotation databases

EnsembliENST00000263863; ENSP00000263863; ENSG00000115523. [P22749-1]
ENST00000409696; ENSP00000387116; ENSG00000115523. [P22749-2]
GeneIDi10578.
KEGGihsa:10578.
UCSCiuc002sql.5. human. [P22749-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05044 mRNA. Translation: CAA28715.1.
X54101 mRNA. Translation: CAA38035.1.
M85276 Genomic DNA. Translation: AAA59935.1.
CR541859 mRNA. Translation: CAG46657.1.
BC023576 mRNA. Translation: AAH23576.1.
CCDSiCCDS1984.1. [P22749-1]
CCDS46354.1. [P22749-2]
PIRiA27562.
I54504.
RefSeqiNP_001289687.1. NM_001302758.1.
NP_006424.2. NM_006433.4. [P22749-1]
NP_036615.2. NM_012483.3. [P22749-2]
UniGeneiHs.105806.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1L9LX-ray0.92A63-136[»]
ProteinModelPortaliP22749.
SMRiP22749.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115829. 1 interactor.
STRINGi9606.ENSP00000263863.

Protein family/group databases

TCDBi1.C.35.3.2. the amoebapore (amoebapore) family.

PTM databases

iPTMnetiP22749.
PhosphoSitePlusiP22749.

Polymorphism and mutation databases

BioMutaiGNLY.
DMDMi116242500.

Proteomic databases

PaxDbiP22749.
PeptideAtlasiP22749.
PRIDEiP22749.

Protocols and materials databases

DNASUi10578.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000263863; ENSP00000263863; ENSG00000115523. [P22749-1]
ENST00000409696; ENSP00000387116; ENSG00000115523. [P22749-2]
GeneIDi10578.
KEGGihsa:10578.
UCSCiuc002sql.5. human. [P22749-1]

Organism-specific databases

CTDi10578.
DisGeNETi10578.
GeneCardsiGNLY.
HGNCiHGNC:4414. GNLY.
HPAiCAB025186.
HPA058021.
MIMi188855. gene.
neXtProtiNX_P22749.
OpenTargetsiENSG00000115523.
PharmGKBiPA28793.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410J70I. Eukaryota.
ENOG41117YV. LUCA.
GeneTreeiENSGT00390000002975.
HOGENOMiHOG000112748.
HOVERGENiHBG005866.
InParanoidiP22749.
PhylomeDBiP22749.
TreeFamiTF342210.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000115523-MONOMER.
ReactomeiR-HSA-6803157. Antimicrobial peptides.

Miscellaneous databases

ChiTaRSiGNLY. human.
EvolutionaryTraceiP22749.
GeneWikiiGNLY.
GenomeRNAii10578.
PROiP22749.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000115523.
CleanExiHS_GNLY.
ExpressionAtlasiP22749. baseline and differential.
GenevisibleiP22749. HS.

Family and domain databases

Gene3Di1.10.225.10. 1 hit.
InterProiIPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PfamiPF03489. SapB_2. 1 hit.
[Graphical view]
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
PROSITEiPS50015. SAP_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGNLY_HUMAN
AccessioniPrimary (citable) accession number: P22749
Secondary accession number(s): P09325, Q6GU08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: October 17, 2006
Last modified: November 30, 2016
This is version 158 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.