Reviewed, UniProtKB/Swiss-Prot P22736 (NR4A1_HUMAN)

Names and origin

Protein names

Recommended name:
    Nuclear receptor subfamily 4 group A member 1
Alternative name(s):
    Orphan nuclear receptor HMR
    Early response protein NAK1
    TR3 orphan receptor
    ST-59

Gene names

Name:	NR4A1
Synonyms:	GFRP1, HMR, NAK1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Orphan nuclear receptor.
Subunit structure	Interacts with GADD45GIP1. Ref.5
Subcellular location	Nucleus.
Tissue specificity	Fetal muscle and adult liver, brain and thyroid.
Induction	By growth-stimulating agents.
Sequence similarities	Belongs to the nuclear hormone receptor family. NR4 subfamily. Contains 1 nuclear receptor DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Receptor
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	signal transduction Ref.2 Traceable author statement. Source: ProtInc transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleoplasm Inferred from Experiment. Source: Reactome
Molecular function	steroid hormone receptor activity Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BCL2	P10415	3	EBI-721550,EBI-77694
BCL2A1	Q16548	1	EBI-721550,EBI-1003550
BCL2L10	Q9HD36	1	EBI-721550,EBI-2126349
E2	P03120	2	EBI-721550,EBI-1779322	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 598

598

Nuclear receptor subfamily 4 group A member 1

PRO_0000053715

Regions

DNA binding

264 – 339

76

Nuclear receptor

Zinc finger

267 – 287

21

NR C4-type

Zinc finger

303 – 327

25

NR C4-type

Region

409 – 459

51

Ligand-binding Potential

Compositional bias

82 – 92

11

Poly-Ser

Compositional bias

583 – 586

4

Poly-Pro

Amino acid modifications

Modified residue

341

1

Phosphoserine; by PKA By similarity

Modified residue

351

1

Phosphoserine Ref.6

Experimental info

Sequence conflict

253

1

T → I Ref.4

Sequence conflict

262

1

G → P in AAA36763. Ref.2

Sequence conflict

370

1

R → L in AAA36763. Ref.2

Secondary structure

1

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598

Helix Strand Turn

Details...

Helix

364 – 373

10

Beta strand

379 – 381

3

Helix

400 – 422

23

Turn

425 – 429

5

Helix

432 – 454

23

Helix

457 – 459

3

Beta strand

461 – 463

3

Beta strand

469 – 471

3

Helix

472 – 479

8

Helix

482 – 495

14

Helix

500 – 511

12

Helix

521 – 541

21

Helix

552 – 556

5

Helix

558 – 579

22

Helix

586 – 593

8

Sequences

	Sequence		Length	Mass (Da)
	P22736-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 41DAAEA7C25FDA22	FASTA	598	64,463
	10 20 30 40 50 60 MPCIQAQYGT PAPSPGPRDH LASDPLTPEF IKPTMDLASP EAAPAAPTAL PSFSTFMDGY 70 80 90 100 110 120 TGEFDTFLYQ LPGTVQPCSS ASSSASSTSS SSATSPASAS FKFEDFQVYG CYPGPLSGPV 130 140 150 160 170 180 DEALSSSGSD YYGSPCSAPS PSTPSFQPPQ LSPWDGSFGH FSPSQTYEGL RAWTEQLPKA 190 200 210 220 230 240 SGPPQPPAFF SFSPPTGPSP SLAQSPLKLF PSQATHQLGE GESYSMPTAF PGLAPTSPHL 250 260 270 280 290 300 EGSGILDTPV TSTKARSGAP GGSEGRCAVC GDNASCQHYG VRTCEGCKGF FKRTVQKNAK 310 320 330 340 350 360 YICLANKDCP VDKRRRNRCQ FCRFQKCLAV GMVKEVVRTD SLKGRRGRLP SKPKQPPDAS 370 380 390 400 410 420 PANLLTSLVR AHLDSGPSTA KLDYSKFQEL VLPHFGKEDA GDVQQFYDLL SGSLEVIRKW 430 440 450 460 470 480 AEKIPGFAEL SPADQDLLLE SAFLELFILR LAYRSKPGEG KLIFCSGLVL HRLQCARGFG 490 500 510 520 530 540 DWIDSILAFS RSLHSLLVDV PAFACLSALV LITDRHGLQE PRRVEELQNR IASCLKEHVA 550 560 570 580 590 AVAGEPQPAS CLSRLLGKLP ELRTLCTQGL QRIFYLKLED LVPPPPIIDK IFMDTLPF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A human early response gene homologous to murine nur77 and rat NGFI-B, and related to the nuclear receptor superfamily." Nakai A., Kartha S., Sakurai A., Toback F.G., Degroot L.J. Mol. Endocrinol. 4:1438-1443(1990) [PubMed: 2283997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Fetal skeletal muscle.
[2]	"Isolation and characterization of human TR3 receptor: a member of steroid receptor superfamily." Chang C., Kokontis J., Liao S., Chang Y. J. Steroid Biochem. 34:391-395(1989) [PubMed: 2626032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[4]	"Phorbol ester, forskolin, and serum induction of a human colon nuclear hormone receptor gene related to the NUR 77/NGFI-B genes." Bondy G.P. Cell Growth Differ. 2:203-208(1991) [PubMed: 1651101] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 35-398. Tissue: Colon adenocarcinoma.
[5]	"CR6-interacting factor 1 interacts with orphan nuclear receptor Nur77 and inhibits its transactivation." Park K.C., Song K.-H., Chung H.K., Kim H., Kim D.W., Song J.H., Hwang E.S., Jung H.S., Park S.-H., Bae I., Lee I.K., Choi H.-S., Shong M. Mol. Endocrinol. 19:12-24(2005) [PubMed: 15459248] [Abstract] Cited for: INTERACTION WITH GADD45GIP1.
[6]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-351, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D49728 mRNA. Translation: BAA08565.1.
L13740 mRNA. Translation: AAA36763.1.
BC016147 mRNA. Translation: AAH16147.1.

IPI

IPI00743108.

PIR

A37251.

RefSeq

NP_002126.2.
NP_775180.1.

UniGene

Hs.524430

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2QW4	X-ray	2.80	A/B/C/D	347-598	[»]

SMR

P22736. Positions 265-353.

ModBase

Search...

Protein-protein interaction databases

IntAct

P22736. 8 interactions.

PTM databases

PhosphoSite

P22736.

Proteomic databases

PRIDE

P22736.

Genome annotation databases

Ensembl

ENSG00000123358. Homo sapiens. [Contig view]

GeneID

3164.

KEGG

hsa:3164.

Organism-specific databases

GeneCards

GC12P050717.

H-InvDB

HIX0010646.

HGNC

HGNC:7980. NR4A1.

MIM

139139. gene.

PharmGKB

PA31761.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

P22736.

Enzyme and pathway databases

Pathway_Interaction_DB

nfat_3pathway. Role of Calcineurin-dependent NFAT signaling in lymphocytes.
rxr_vdr_pathway. RXR and RAR hetrodimerization with other nuclear receptor.

Reactome

REACT_11061. Signalling by NGF.

Gene expression databases

ArrayExpress

P22736.

Bgee

P22736.

CleanEx

HS_NR4A1.

GermOnline

ENSG00000123358. Homo sapiens.

Family and domain databases

InterPro

IPR003071. Nuc_orp_HMR_rcpt.
IPR003070. Nuc_orph_rcpt.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]

Gene3D

G3DSA:1.10.565.10. Nucl_hrmn_rcpt_lig_bd. 1 hit.
G3DSA:3.30.50.10. Znf_NHR/GATA. 1 hit.

Pfam

PF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]

PRINTS

PR01285. HMRNUCRECPTR.
PR01284. NUCLEARECPTR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.

ProDom

PD000035. Znf_C4steroid. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]

PROSITE

PS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

12546.

SOURCE

Search...

Entry information

Entry name

NR4A1_HUMAN

Accession

Primary (citable) accession number: P22736

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	June 16, 2009
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)