P22732 (GTR5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Solute carrier family 2, facilitated glucose transporter member 5

Alternative name(s):
Fructose transporter
Glucose transporter type 5, small intestine
Short name=GLUT-5

Gene names

Name:	SLC2A5
Synonyms:	GLUT5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochalasin B-sensitive carrier. Seems to function primarily as a fructose transporter.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue.
Induction	By forskolin in Caco-2 cells. Ref.2
Sequence similarities	Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. [View classification]
Mass spectrometry	Molecular mass is 54973.98 Da from positions 1 - 501. Determined by MALDI. Ref.7
Sequence caution	The sequence AAB60641.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Sugar transport Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Traceable author statement. Source: Reactome small molecule metabolic process Traceable author statement. Source: Reactome
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Molecular_function	fructose transmembrane transporter activity Traceable author statement PubMed 1634504. Source: ProtInc glucose transmembrane transporter activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P22732-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P22732-2)

The sequence of this isoform differs from the canonical sequence as follows:
     192-501: WPILLGLTGV...KELPPVTSEQ → EFRTSREHPH...LGGPSCPEPT

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Solute carrier family 2, facilitated glucose transporter member 5		PRO_0000050369
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 33	21	Helical; Name=1; Potential
	Topological domain	34 – 68	35	Extracellular Potential
	Transmembrane	69 – 89	21	Helical; Name=2; Potential
	Topological domain	90 – 98	9	Cytoplasmic Potential
	Transmembrane	99 – 119	21	Helical; Name=3; Potential
	Topological domain	120	1	Extracellular Potential
	Transmembrane	121 – 141	21	Helical; Name=4; Potential
	Topological domain	142 – 161	20	Cytoplasmic Potential
	Transmembrane	162 – 182	21	Helical; Name=5; Potential
	Topological domain	183 – 192	10	Extracellular Potential
	Transmembrane	193 – 213	21	Helical; Name=6; Potential
	Topological domain	214 – 277	64	Cytoplasmic Potential
	Transmembrane	278 – 298	21	Helical; Name=7; Potential
	Topological domain	299 – 318	20	Extracellular Potential
	Transmembrane	319 – 339	21	Helical; Name=8; Potential
	Topological domain	340 – 342	3	Cytoplasmic Potential
	Transmembrane	343 – 363	21	Helical; Name=9; Potential
	Topological domain	364 – 368	5	Extracellular Potential
	Transmembrane	369 – 389	21	Helical; Name=10; Potential
	Topological domain	390 – 412	23	Cytoplasmic Potential
	Transmembrane	413 – 433	21	Helical; Name=11; Potential
	Topological domain	434 – 439	6	Extracellular Potential
	Transmembrane	440 – 460	21	Helical; Name=12; Potential
	Topological domain	461 – 501	41	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	51	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	192 – 501	310	WPILL…VTSEQ → EFRTSREHPHPFTTTLGPLL VFQSHHHRTGLSADWSLLTG WMSLGGPSCPEPT in isoform 2.		VSP_042031
Experimental info
	Sequence conflict	202	1	P → G in AAB60641. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 090B12F529C7B857	FASTA	501	54,974
	10 20 30 40 50 60 MEQQDQSMKE GRLTLVLALA TLIAAFGSSF QYGYNVAAVN SPALLMQQFY NETYYGRTGE 70 80 90 100 110 120 FMEDFPLTLL WSVTVSMFPF GGFIGSLLVG PLVNKFGRKG ALLFNNIFSI VPAILMGCSR 130 140 150 160 170 180 VATSFELIII SRLLVGICAG VSSNVVPMYL GELAPKNLRG ALGVVPQLFI TVGILVAQIF 190 200 210 220 230 240 GLRNLLANVD GWPILLGLTG VPAALQLLLL PFFPESPRYL LIQKKDEAAA KKALQTLRGW 250 260 270 280 290 300 DSVDREVAEI RQEDEAEKAA GFISVLKLFR MRSLRWQLLS IIVLMGGQQL SGVNAIYYYA 310 320 330 340 350 360 DQIYLSAGVP EEHVQYVTAG TGAVNVVMTF CAVFVVELLG RRLLLLLGFS ICLIACCVLT 370 380 390 400 410 420 AALALQDTVS WMPYISIVCV ISYVIGHALG PSPIPALLIT EIFLQSSRPS AFMVGGSVHW 430 440 450 460 470 480 LSNFTVGLIF PFIQEGLGPY SFIVFAVICL LTTIYIFLIV PETKAKTFIE INQIFTKMNK 490 500 VSEVYPEKEE LKELPPVTSE Q
	Isoform 2 [UniParc]. Checksum: 310B5F011D6CEA71	FASTA	244	26,519
	10 20 30 40 50 60 MEQQDQSMKE GRLTLVLALA TLIAAFGSSF QYGYNVAAVN SPALLMQQFY NETYYGRTGE 70 80 90 100 110 120 FMEDFPLTLL WSVTVSMFPF GGFIGSLLVG PLVNKFGRKG ALLFNNIFSI VPAILMGCSR 130 140 150 160 170 180 VATSFELIII SRLLVGICAG VSSNVVPMYL GELAPKNLRG ALGVVPQLFI TVGILVAQIF 190 200 210 220 230 240 GLRNLLANVD GEFRTSREHP HPFTTTLGPL LVFQSHHHRT GLSADWSLLT GWMSLGGPSC PEPT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human facilitative glucose transporters. Isolation, functional characterization, and gene localization of cDNAs encoding an isoform (GLUT5) expressed in small intestine, kidney, muscle, and adipose tissue and an unusual glucose transporter pseudogene-like sequence (GLUT6)." Kayano T., Burant C.F., Fukumoto H., Gould G.W., Fan Y.-S., Eddy R.L., Byers M.G., Shows T.B., Seino S., Bell G.I. J. Biol. Chem. 265:13276-13282(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Jejunum.
[2]	"Regulation of expression of the human fructose transporter (GLUT5) by cyclic AMP." Mahraoui L., Takeda J., Mesonero J., Chantret I., Dussaulx E., Bell G.I., Brot-Laroche E. Biochem. J. 301:169-175(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION. Tissue: Fetal liver.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Brain and Umbilical cord blood.
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Lymph and Prostate.
[7]	"Cluster analysis of an extensive human breast cancer cell line protein expression map database." Harris R.A., Yang A., Stein R.C., Lucy K., Brusten L., Herath A., Parekh R., Waterfield M.D., O'Hare M.J., Neville M.A., Page M.J., Zvelebil M.J. Proteomics 2:212-223(2002) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY. Tissue: Mammary cancer.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M55531 mRNA. Translation: AAA52570.1.
U11843 , U05344, U11839, U11840, U11841, U11842 Genomic DNA. Translation: AAB60641.1. Sequence problems.
AK289849 mRNA. Translation: BAF82538.1.
AK290398 mRNA. Translation: BAF83087.1.
AL158048 Genomic DNA. Translation: CAI23457.1.
AL158048 Genomic DNA. Translation: CAI23458.1.
CH471130 Genomic DNA. Translation: EAW71610.1.
BC001692 mRNA. Translation: AAH01692.1.
BC001820 mRNA. Translation: AAH01820.1.
BC035878 mRNA. Translation: AAH35878.1.

IPI

IPI00027452.

PIR

A36629.
G02864.

RefSeq

NP_001129057.1. NM_001135585.1.
NP_003030.1. NM_003039.2.

UniGene

Hs.530003.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YG1	model	-	A	1-501	[»]

ProteinModelPortal

P22732.

ModBase

Search...

Protein-protein interaction databases

IntAct

P22732. 3 interactions.

STRING

9606.ENSP00000366641.

Protein family/group databases

TCDB

2.A.1.1.13. major facilitator superfamily (MFS).

PTM databases

PhosphoSite

P22732.

Polymorphism databases

DMDM

121764.

Proteomic databases

PaxDb

P22732.

PeptideAtlas

P22732.

PRIDE

P22732.

Protocols and materials databases

DNASU

6518.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000377414; ENSP00000366631; ENSG00000142583.
ENST00000377424; ENSP00000366641; ENSG00000142583.

GeneID

6518.

KEGG

hsa:6518.

UCSC

uc001apo.3. human.
uc001app.4. human.

Organism-specific databases

CTD

6518.

GeneCards

GC01M009095.

HGNC

HGNC:11010. SLC2A5.

HPA

CAB026176.
HPA005449.

MIM

138230. gene.

neXtProt

NX_P22732.

PharmGKB

PA35880.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG0477.

HOGENOM

HOG000202871.

HOVERGEN

HBG014816.

InParanoid

P22732.

KO

K08143.

OMA

RMRSLRW.

OrthoDB

EOG42Z4Q3.

PhylomeDB

P22732.

Enzyme and pathway databases

Reactome

REACT_111217. Metabolism.
REACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpress

P22732.

Bgee

P22732.

CleanEx

HS_SLC2A5.

Genevestigator

P22732.

GermOnline

ENSG00000142583. Homo sapiens.

Family and domain databases

InterPro

IPR002442. Fru_transpt_5.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]

Pfam

PF00083. Sugar_tr. 1 hit.
[Graphical view]

PRINTS

PR01194. GLUCTRSPORT5.
PR00171. SUGRTRNSPORT.

SUPFAM

SSF103473. MFS_gen_substrate_transporter. 1 hit.

TIGRFAMs

TIGR00879. SP. 1 hit.

PROSITE

PS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P22732.

ChEMBL

CHEMBL5875.

ChiTaRS

SLC2A5. human.

GenomeRNAi

6518.

NextBio

25345.

SOURCE

Search...

Entry information

Entry name

GTR5_HUMAN

Accession

Primary (citable) accession number: P22732
Secondary accession number(s): Q14770, Q5T977, Q8IVB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	May 29, 2013
This is version 137 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.