P22727 (WNT6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-6

Gene names

Name:	Wnt6
Synonyms:	Wnt-6

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Subunit structure	Interacts with PORCN. Ref.5
Subcellular location	Secreted › extracellular space › extracellular matrix.
Post-translational modification	Palmitoylation at Ser-227 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-75. Palmitoylation is necessary for proper trafficking to cell surface By similarity.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anterior/posterior pattern specification Inferred from Biological aspect of Ancestor. Source: RefGenome axis specification Inferred from direct assay PubMed 11948913. Source: MGI branching involved in ureteric bud morphogenesis Inferred from genetic interaction PubMed 11948913. Source: MGI canonical Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome epithelial-mesenchymal cell signaling Inferred from direct assay PubMed 11948913. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from Biological aspect of Ancestor. Source: RefGenome nephron tubule formation Inferred from direct assay PubMed 11948913. Source: MGI odontogenesis of dentin-containing tooth Inferred from electronic annotation. Source: Compara positive regulation of tooth mineralization Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from direct assay PubMed 9356179. Source: MGI
Cellular_component	cell surface Inferred from direct assay PubMed 8167409. Source: BHF-UCL extracellular matrix Inferred from direct assay PubMed 8167409. Source: MGI extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 364	341	Protein Wnt-6		PRO_0000041441
Amino acid modifications
	Lipidation	75	1	S-palmitoyl cysteine By similarity
	Lipidation	227	1	O-palmitoyl serine; by PORCN By similarity
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	310	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	233	1	R → S in AAA40569. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P22727 [UniParc]. Last modified June 28, 2011. Version 2. Checksum: 6F28CE191F98A0AC	FASTA	364	39,656
	10 20 30 40 50 60 MLPPVPSRLG LLLLLLCPAH VDGLWWAVGS PLVMDPTSIC RKARRLAGRQ AELCQAEPEV 70 80 90 100 110 120 VAELARGARL GVRECQFQFR FRRWNCSSHS KAFGRVLQQD IRETAFVFAI TAAGASHAVT 130 140 150 160 170 180 QACSMGELLQ CGCQAPRGRA PPRPSGLLGT PGPPGPTGSP DASAAWEWGG CGDDVDFGDE 190 200 210 220 230 240 KSRLFMDAQH KRGRGDIRAL VQLHNNEAGR LAVRSHTRTE CKCHGLSGSC ALRTCWQKLP 250 260 270 280 290 300 PFREVGARLL ERFHGASRVM GTNDGKALLP AVRTLKPPGR ADLLYAADSP DFCAPNRRTG 310 320 330 340 350 360 SPGTRGRACN SSAPDLSGCD LLCCGRGHRQ ESVQLEENCL CRFHWCCVVQ CHRCRVRKEL SLCL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development." Gavin B.J., McMahon J.A., McMahon A.P. Genes Dev. 4:2319-2332(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Limb.
[5]	"The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family." Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T. Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PORCN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M89800 mRNA. Translation: AAA40569.1. AK133954 mRNA. Translation: BAE21949.1. CH466548 Genomic DNA. Translation: EDL00351.1. BC048700 mRNA. Translation: AAH48700.1.
IPI	IPI00131378.
PIR	F36470.
RefSeq	NP_033552.2. NM_009526.3.
UniGene	Mm.268282.
3D structure databases
ProteinModelPortal	P22727.
SMR	P22727. Positions 62-355.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000006716.
Proteomic databases
PRIDE	P22727.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000006716; ENSMUSP00000006716; ENSMUSG00000033227.
GeneID	22420.
KEGG	mmu:22420.
Organism-specific databases
CTD	7475.
MGI	MGI:98960. Wnt6.
Phylogenomic databases
eggNOG	NOG246444.
GeneTree	ENSGT00690000101771.
HOGENOM	HOG000039528.
HOVERGEN	HBG001595.
InParanoid	Q80ZM9.
KO	K00445.
OMA	RTLKPPG.
OrthoDB	EOG4BZN2W.
Gene expression databases
Bgee	P22727.
CleanEx	MM_WNT6.
Genevestigator	P22727.
GermOnline	ENSMUSG00000033227. Mus musculus.
Family and domain databases
InterPro	IPR005817. Wnt. IPR009143. Wnt6. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF14. PTHR12027:SF14. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01845. WNT6PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302849.
SOURCE	Search...

Entry information

Entry name

WNT6_MOUSE

Accession

Primary (citable) accession number: P22727
Secondary accession number(s): Q80ZM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	June 28, 2011
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program