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Protein

Protein Wnt-5b

Gene

Wnt5b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

GO - Molecular functioni

  1. frizzled binding Source: GO_Central
  2. receptor binding Source: MGI

GO - Biological processi

  1. cell-cell signaling Source: MGI
  2. cell fate commitment Source: GO_Central
  3. chondrocyte differentiation Source: Ensembl
  4. negative regulation of canonical Wnt signaling pathway Source: MGI
  5. neuron differentiation Source: GO_Central
  6. organ morphogenesis Source: MGI
  7. positive regulation of cell migration Source: MGI
  8. positive regulation of fat cell differentiation Source: MGI
  9. signal transduction Source: MGI
  10. Wnt signaling pathway Source: MGI
  11. wound healing Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiREACT_295052. WNT ligand biogenesis and trafficking.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-5b
Gene namesi
Name:Wnt5b
Synonyms:Wnt-5b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:98959. Wnt5b.

Subcellular locationi

GO - Cellular componenti

  1. cell surface Source: BHF-UCL
  2. endoplasmic reticulum lumen Source: Reactome
  3. extracellular space Source: GO_Central
  4. extracellular vesicular exosome Source: MGI
  5. proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 359342Protein Wnt-5bPRO_0000041435Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi83 ↔ 94By similarity
Glycosylationi93 – 931N-linked (GlcNAc...)Sequence Analysis
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi133 ↔ 141By similarity
Disulfide bondi143 ↔ 161By similarity
Disulfide bondi217 ↔ 231By similarity
Disulfide bondi219 ↔ 226By similarity
Lipidationi223 – 2231O-palmitoyl serine; by PORCNBy similarity
Glycosylationi291 – 2911N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi304 ↔ 319By similarity
Glycosylationi305 – 3051N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi334 ↔ 349By similarity
Disulfide bondi336 ↔ 346By similarity
Disulfide bondi341 ↔ 342By similarity

Post-translational modificationi

Palmitoylation at Ser-223 is required for efficient binding to frizzled receptors. Palmitoylation is necessary for proper trafficking to cell surface (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PRIDEiP22726.

PTM databases

PhosphoSiteiP22726.

Expressioni

Gene expression databases

BgeeiP22726.
CleanExiMM_WNT5B.
ExpressionAtlasiP22726. baseline and differential.
GenevestigatoriP22726.

Interactioni

Subunit structurei

Interacts with PORCN.1 Publication

Protein-protein interaction databases

BioGridi204578. 1 interaction.
STRINGi10090.ENSMUSP00000032273.

Structurei

3D structure databases

ProteinModelPortaliP22726.
SMRiP22726. Positions 73-288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG284879.
GeneTreeiENSGT00760000118943.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiP22726.
KOiK00444.
OrthoDBiEOG7C8GJ8.
PhylomeDBiP22726.
TreeFamiTF105310.

Family and domain databases

InterProiIPR005817. Wnt.
IPR026537. Wnt5b.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF87. PTHR12027:SF87. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSLLLVVVA ALLSSWAQLL TDANSWWSLA LNPVQRPEMF IIGAQPVCSQ
60 70 80 90 100
LPGLSPGQRK LCQLYQEHMS YIGEGAKTGI RECQHQFRQR RWNCSTVDNT
110 120 130 140 150
SVFGRVMQIG SRETAFTYAV SAAGVVNAIS RACREGELST CGCSRAARPK
160 170 180 190 200
DLPRDWLWGG CGDNVEYGYR FAKEFVDARE REKNFAKGSE EQGRALMNLQ
210 220 230 240 250
NNEAGRRAVY KMADVACKCH GVSGSCSLKT CWLQLAEFRK VGDRLKEKYD
260 270 280 290 300
SAAAMRITRQ GKLELANSRF NQPTPEDLVY VDPSPDYCLR NETTGSLGTQ
310 320 330 340 350
GRLCNKTSEG MDGCELMCCG RGYDRFKSVQ VERCHCRFHW CCFVRCKKCT

EVVDQYVCK
Length:359
Mass (Da):40,343
Last modified:June 20, 2001 - v2
Checksum:i308ED393D3020DEB
GO

Sequence cautioni

The sequence AAA40568.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence AAH10775.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89799 mRNA. Translation: AAA40568.1. Different initiation.
BC010775 mRNA. Translation: AAH10775.1. Different initiation.
BC051406 mRNA. Translation: AAH51406.1.
CCDSiCCDS20474.2.
PIRiE36470.
RefSeqiNP_001258686.1. NM_001271757.1.
NP_001258687.1. NM_001271758.1.
NP_033551.2. NM_009525.3.
XP_006505987.1. XM_006505924.1.
UniGeneiMm.321818.

Genome annotation databases

EnsembliENSMUST00000117171; ENSMUSP00000113188; ENSMUSG00000030170.
ENSMUST00000178696; ENSMUSP00000137065; ENSMUSG00000030170.
GeneIDi22419.
KEGGimmu:22419.
UCSCiuc009dmg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89799 mRNA. Translation: AAA40568.1. Different initiation.
BC010775 mRNA. Translation: AAH10775.1. Different initiation.
BC051406 mRNA. Translation: AAH51406.1.
CCDSiCCDS20474.2.
PIRiE36470.
RefSeqiNP_001258686.1. NM_001271757.1.
NP_001258687.1. NM_001271758.1.
NP_033551.2. NM_009525.3.
XP_006505987.1. XM_006505924.1.
UniGeneiMm.321818.

3D structure databases

ProteinModelPortaliP22726.
SMRiP22726. Positions 73-288.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204578. 1 interaction.
STRINGi10090.ENSMUSP00000032273.

PTM databases

PhosphoSiteiP22726.

Proteomic databases

PRIDEiP22726.

Protocols and materials databases

DNASUi22419.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000117171; ENSMUSP00000113188; ENSMUSG00000030170.
ENSMUST00000178696; ENSMUSP00000137065; ENSMUSG00000030170.
GeneIDi22419.
KEGGimmu:22419.
UCSCiuc009dmg.2. mouse.

Organism-specific databases

CTDi81029.
MGIiMGI:98959. Wnt5b.

Phylogenomic databases

eggNOGiNOG284879.
GeneTreeiENSGT00760000118943.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiP22726.
KOiK00444.
OrthoDBiEOG7C8GJ8.
PhylomeDBiP22726.
TreeFamiTF105310.

Enzyme and pathway databases

ReactomeiREACT_295052. WNT ligand biogenesis and trafficking.

Miscellaneous databases

NextBioi302845.
PROiP22726.
SOURCEiSearch...

Gene expression databases

BgeeiP22726.
CleanExiMM_WNT5B.
ExpressionAtlasiP22726. baseline and differential.
GenevestigatoriP22726.

Family and domain databases

InterProiIPR005817. Wnt.
IPR026537. Wnt5b.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF87. PTHR12027:SF87. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development."
    Gavin B.J., McMahon J.A., McMahon A.P.
    Genes Dev. 4:2319-2332(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Liver and Mammary gland.
  3. "The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family."
    Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T.
    Eur. J. Biochem. 267:4300-4311(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PORCN.

Entry informationi

Entry nameiWNT5B_MOUSE
AccessioniPrimary (citable) accession number: P22726
Secondary accession number(s): Q91XF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: June 20, 2001
Last modified: April 1, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.