P22726 (WNT5B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-5b

Gene names

Name:	Wnt5b
Synonyms:	Wnt-5b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Subunit structure	Interacts with PORCN. Ref.3
Subcellular location	Secreted › extracellular space › extracellular matrix.
Post-translational modification	Palmitoylation at Ser-223 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-83. Palmitoylation is necessary for proper trafficking to cell surface By similarity.
Sequence similarities	Belongs to the Wnt family.
Sequence caution	The sequence AAA40568.1 differs from that shown. Reason: Erroneous initiation. The sequence AAH10775.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway, calcium modulating pathway Inferred from Biological aspect of Ancestor. Source: RefGenome angiogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome anterior/posterior pattern specification Inferred from Biological aspect of Ancestor. Source: RefGenome cell migration involved in gastrulation Inferred from Biological aspect of Ancestor. Source: RefGenome cell-cell signaling Traceable author statement PubMed 9889131. Source: MGI chondrocyte differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome convergent extension involved in axis elongation Inferred from Biological aspect of Ancestor. Source: RefGenome convergent extension involved in gastrulation Inferred from Biological aspect of Ancestor. Source: RefGenome dorsal/ventral axis specification Inferred from Biological aspect of Ancestor. Source: RefGenome endocrine pancreas development Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of canonical Wnt receptor signaling pathway Inferred from genetic interaction PubMed 15796911. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from Biological aspect of Ancestor. Source: RefGenome organ morphogenesis Traceable author statement PubMed 9889131. Source: MGI positive regulation of cell migration Inferred from electronic annotation. Source: Compara positive regulation of fat cell differentiation Inferred from genetic interaction PubMed 15796911. Source: MGI respiratory system development Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cell surface Inferred from direct assay PubMed 8167409. Source: BHF-UCL extracellular matrix Inferred from direct assay PubMed 8167409. Source: MGI extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	frizzled-2 binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 359	342	Protein Wnt-5b		PRO_0000041435
Amino acid modifications
	Lipidation	83	1	S-palmitoyl cysteine By similarity
	Lipidation	223	1	O-palmitoyl serine; by PORCN By similarity
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	291	1	N-linked (GlcNAc...) Potential
	Glycosylation	305	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P22726 [UniParc]. Last modified June 20, 2001. Version 2. Checksum: 308ED393D3020DEB	FASTA	359	40,343
	10 20 30 40 50 60 MPSLLLVVVA ALLSSWAQLL TDANSWWSLA LNPVQRPEMF IIGAQPVCSQ LPGLSPGQRK 70 80 90 100 110 120 LCQLYQEHMS YIGEGAKTGI RECQHQFRQR RWNCSTVDNT SVFGRVMQIG SRETAFTYAV 130 140 150 160 170 180 SAAGVVNAIS RACREGELST CGCSRAARPK DLPRDWLWGG CGDNVEYGYR FAKEFVDARE 190 200 210 220 230 240 REKNFAKGSE EQGRALMNLQ NNEAGRRAVY KMADVACKCH GVSGSCSLKT CWLQLAEFRK 250 260 270 280 290 300 VGDRLKEKYD SAAAMRITRQ GKLELANSRF NQPTPEDLVY VDPSPDYCLR NETTGSLGTQ 310 320 330 340 350 GRLCNKTSEG MDGCELMCCG RGYDRFKSVQ VERCHCRFHW CCFVRCKKCT EVVDQYVCK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development." Gavin B.J., McMahon J.A., McMahon A.P. Genes Dev. 4:2319-2332(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N-3. Tissue: Liver and Mammary gland.
[3]	"The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family." Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T. Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PORCN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M89799 mRNA. Translation: AAA40568.1. Different initiation. BC010775 mRNA. Translation: AAH10775.1. Different initiation. BC051406 mRNA. Translation: AAH51406.1.
IPI	IPI00129100.
PIR	E36470.
RefSeq	NP_001258686.1. NM_001271757.1. NP_001258687.1. NM_001271758.1. NP_033551.2. NM_009525.3.
UniGene	Mm.321818.
3D structure databases
ProteinModelPortal	P22726.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000032273.
PTM databases
PhosphoSite	P22726.
Proteomic databases
PRIDE	P22726.
Protocols and materials databases
DNASU	22419.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000117171; ENSMUSP00000113188; ENSMUSG00000030170. ENSMUST00000119369; ENSMUSP00000112448; ENSMUSG00000030170. ENSMUST00000178696; ENSMUSP00000137065; ENSMUSG00000030170.
GeneID	22419.
KEGG	mmu:22419.
UCSC	uc009dmg.2. mouse.
Organism-specific databases
CTD	81029.
MGI	MGI:98959. Wnt5b.
Phylogenomic databases
eggNOG	NOG284879.
GeneTree	ENSGT00700000104304.
HOGENOM	HOG000039529.
HOVERGEN	HBG001595.
InParanoid	P22726.
KO	K00444.
OrthoDB	EOG4H19VP.
Gene expression databases
ArrayExpress	P22726.
Bgee	P22726.
CleanEx	MM_WNT5B.
Genevestigator	P22726.
GermOnline	ENSMUSG00000030170. Mus musculus.
Family and domain databases
InterPro	IPR005817. Wnt. IPR026537. Wnt5b. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF23. PTHR12027:SF23. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302845.
SOURCE	Search...

Entry information

Entry name

WNT5B_MOUSE

Accession

Primary (citable) accession number: P22726
Secondary accession number(s): Q91XF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	June 20, 2001
Last modified:	April 3, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program