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P22726 (WNT5B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein Wnt-5b
Gene names
Name:Wnt5b
Synonyms:Wnt-5b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

Subunit structure

Interacts with PORCN. Ref.3

Subcellular location

Secretedextracellular spaceextracellular matrix.

Post-translational modification

Palmitoylation at Ser-223 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-83. Palmitoylation is necessary for proper trafficking to cell surface By similarity.

Sequence similarities

Belongs to the Wnt family.

Sequence caution

The sequence AAA40568.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAH10775.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processWnt signaling pathway
   Cellular componentExtracellular matrix
Secreted
   DomainSignal
   Molecular functionDevelopmental protein
   PTMGlycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

cell fate commitment

Inferred from Biological aspect of Ancestor. Source: RefGenome

cell-cell signaling

Traceable author statement PubMed 9889131. Source: MGI

chondrocyte differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of canonical Wnt signaling pathway

Inferred from genetic interaction PubMed 15796911. Source: MGI

neuron differentiation

Inferred from Biological aspect of Ancestor. Source: RefGenome

organ morphogenesis

Traceable author statement PubMed 9889131. Source: MGI

positive regulation of cell migration

Inferred from electronic annotation. Source: Ensembl

positive regulation of fat cell differentiation

Inferred from genetic interaction PubMed 15796911. Source: MGI

signal transduction

Traceable author statement PubMed 9889131. Source: MGI

wound healing

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell surface

Inferred from direct assay PubMed 8167409. Source: BHF-UCL

endoplasmic reticulum lumen

Traceable author statement. Source: Reactome

extracellular matrix

Inferred from direct assay PubMed 8167409. Source: MGI

extracellular space

Inferred from Biological aspect of Ancestor. Source: RefGenome

proteinaceous extracellular matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionfrizzled binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

receptor binding

Traceable author statement PubMed 9889131. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 359342Protein Wnt-5b
PRO_0000041435

Amino acid modifications

Lipidation831S-palmitoyl cysteine By similarity
Lipidation2231O-palmitoyl serine; by PORCN By similarity
Glycosylation931N-linked (GlcNAc...) Potential
Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation2911N-linked (GlcNAc...) Potential
Glycosylation3051N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P22726 [UniParc].

Last modified June 20, 2001. Version 2.
Checksum: 308ED393D3020DEB

FASTA35940,343
        10         20         30         40         50         60 
MPSLLLVVVA ALLSSWAQLL TDANSWWSLA LNPVQRPEMF IIGAQPVCSQ LPGLSPGQRK 

        70         80         90        100        110        120 
LCQLYQEHMS YIGEGAKTGI RECQHQFRQR RWNCSTVDNT SVFGRVMQIG SRETAFTYAV 

       130        140        150        160        170        180 
SAAGVVNAIS RACREGELST CGCSRAARPK DLPRDWLWGG CGDNVEYGYR FAKEFVDARE 

       190        200        210        220        230        240 
REKNFAKGSE EQGRALMNLQ NNEAGRRAVY KMADVACKCH GVSGSCSLKT CWLQLAEFRK 

       250        260        270        280        290        300 
VGDRLKEKYD SAAAMRITRQ GKLELANSRF NQPTPEDLVY VDPSPDYCLR NETTGSLGTQ 

       310        320        330        340        350 
GRLCNKTSEG MDGCELMCCG RGYDRFKSVQ VERCHCRFHW CCFVRCKKCT EVVDQYVCK 

« Hide

References

« Hide 'large scale' references
[1]"Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development."
Gavin B.J., McMahon J.A., McMahon A.P.
Genes Dev. 4:2319-2332(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N-3.
Tissue: Liver and Mammary gland.
[3]"The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family."
Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T.
Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PORCN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M89799 mRNA. Translation: AAA40568.1. Different initiation.
BC010775 mRNA. Translation: AAH10775.1. Different initiation.
BC051406 mRNA. Translation: AAH51406.1.
CCDSCCDS20474.2.
PIRE36470.
RefSeqNP_001258686.1. NM_001271757.1.
NP_001258687.1. NM_001271758.1.
NP_033551.2. NM_009525.3.
XP_006505987.1. XM_006505924.1.
XP_006505988.1. XM_006505925.1.
UniGeneMm.321818.

3D structure databases

ProteinModelPortalP22726.
SMRP22726. Positions 73-288.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204578. 1 interaction.
STRING10090.ENSMUSP00000032273.

PTM databases

PhosphoSiteP22726.

Proteomic databases

PRIDEP22726.

Protocols and materials databases

DNASU22419.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000117171; ENSMUSP00000113188; ENSMUSG00000030170.
ENSMUST00000119369; ENSMUSP00000112448; ENSMUSG00000030170.
ENSMUST00000178696; ENSMUSP00000137065; ENSMUSG00000030170.
GeneID22419.
KEGGmmu:22419.
UCSCuc009dmg.2. mouse.

Organism-specific databases

CTD81029.
MGIMGI:98959. Wnt5b.

Phylogenomic databases

eggNOGNOG284879.
GeneTreeENSGT00740000115016.
HOGENOMHOG000039529.
HOVERGENHBG001595.
InParanoidP22726.
KOK00444.
OrthoDBEOG7C8GJ8.
PhylomeDBP22726.
TreeFamTF105310.

Enzyme and pathway databases

ReactomeREACT_188257. Signal Transduction.

Gene expression databases

ArrayExpressP22726.
BgeeP22726.
CleanExMM_WNT5B.
GenevestigatorP22726.

Family and domain databases

InterProIPR005817. Wnt.
IPR026537. Wnt5b.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF87. PTHR12027:SF87. 1 hit.
PfamPF00110. wnt. 1 hit.
[Graphical view]
PRINTSPR01349. WNTPROTEIN.
SMARTSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302845.
PROP22726.
SOURCESearch...

Entry information

Entry nameWNT5B_MOUSE
AccessionPrimary (citable) accession number: P22726
Secondary accession number(s): Q91XF5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: June 20, 2001
Last modified: July 9, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot