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P22725 (WNT5A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein Wnt-5a
Gene names
Name:Wnt5a
Synonyms:Wnt-5a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length380 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. Ref.5 Ref.6 Ref.8 Ref.9

Subunit structure

Homooligomer; disulfide-linked, leading to inactivation. Interacts with PORCN. Interacts with WLS. Ref.7 Ref.10 Ref.11

Subcellular location

Secretedextracellular spaceextracellular matrix.

Tissue specificity

Expressed in a gradient at the caudal end of the embryo during gastrulation and later in the distal-most aspect of several structures that extend from the body such as the limbs and genital tubercle. Ref.6

Post-translational modification

Palmitoylation is necessary for stimulation of cell migration, inhibition of the beta-catenin pathway and receptor binding. Ref.9

Glycosylation is necessary for secretion but not for activity.

Palmitoylation at Ser-244 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-104. Palmitoylation is necessary for proper trafficking to cell surface By similarity. Ref.9

Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT5A.

Disruption phenotype

Mice display perinatal lethality and display gross morphological defects in outgrowing tissues. They are truncated caudally, displaying loss of the tail and a significant shortening of the anterior-posterior axis. The shape of the head is abnormal with truncated snout, mandible and tongue and reduced outgrowth of the external ear. Fore- and hindlimbs lack digits, the genital tubercle is missing and chondrocyte differentiation is inhibited. Ref.6 Ref.8

Sequence similarities

Belongs to the Wnt family.

Ontologies

Keywords
   Biological processChondrogenesis
Differentiation
Wnt signaling pathway
   Cellular componentExtracellular matrix
Secreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionDevelopmental protein
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processJNK cascade

Inferred from direct assay PubMed 12839624. Source: MGI

Wnt signaling pathway

Inferred from genetic interaction PubMed 11092808PubMed 16115200. Source: MGI

Wnt signaling pathway, calcium modulating pathway

Inferred from direct assay PubMed 18174455. Source: BHF-UCL

Wnt signaling pathway, planar cell polarity pathway

Inferred from direct assay PubMed 19056682. Source: MGI

activation of JUN kinase activity

Inferred from electronic annotation. Source: Ensembl

activation of protein kinase B activity

Inferred from electronic annotation. Source: Ensembl

ameboidal cell migration

Inferred from mutant phenotype PubMed 19100728. Source: MGI

anterior/posterior axis specification, embryo

Inferred from direct assay PubMed 9054360. Source: BHF-UCL

anterior/posterior pattern specification

Inferred from mutant phenotype PubMed 15073149PubMed 19795512. Source: MGI

axis elongation

Inferred from mutant phenotype PubMed 19100728PubMed 19795512. Source: MGI

axon guidance

Inferred from direct assay PubMed 21483795. Source: UniProtKB

canonical Wnt signaling pathway

Inferred from direct assay PubMed 18929644. Source: BHF-UCL

cartilage development

Inferred from electronic annotation. Source: UniProtKB-KW

cell fate commitment

Inferred from Biological aspect of Ancestor. Source: RefGenome

cell migration

Inferred from mutant phenotype PubMed 16246260. Source: MGI

cell-cell signaling

Traceable author statement PubMed 9889131. Source: MGI

cellular protein localization

Inferred from mutant phenotype PubMed 17433286. Source: MGI

cellular response to calcium ion

Inferred from electronic annotation. Source: Ensembl

cellular response to interferon-gamma

Inferred from electronic annotation. Source: Ensembl

cellular response to lipopolysaccharide

Inferred from electronic annotation. Source: Ensembl

cellular response to molecule of bacterial origin

Inferred from direct assay PubMed 16601243. Source: BHF-UCL

cellular response to transforming growth factor beta stimulus

Inferred from electronic annotation. Source: Ensembl

cervix development

Inferred from mutant phenotype PubMed 15073149. Source: MGI

cochlea morphogenesis

Inferred from mutant phenotype PubMed 17433286. Source: MGI

convergent extension

Inferred from mutant phenotype PubMed 17433286. Source: MGI

convergent extension involved in organogenesis

Inferred from genetic interaction PubMed 17433286PubMed 19795512. Source: MGI

development of primary male sexual characteristics

Inferred from mutant phenotype PubMed 19100252. Source: MGI

digestive tract morphogenesis

Inferred from mutant phenotype PubMed 19300477. Source: MGI

dopaminergic neuron differentiation

Inferred from mutant phenotype PubMed 18953410. Source: MGI

embryonic digit morphogenesis

Inferred from mutant phenotype PubMed 19795512. Source: MGI

embryonic limb morphogenesis

Inferred from mutant phenotype Ref.6. Source: MGI

embryonic skeletal system development

Inferred from electronic annotation. Source: Ensembl

epithelial cell proliferation involved in mammary gland duct elongation

Inferred from direct assay PubMed 17898001. Source: MGI

epithelial to mesenchymal transition

Inferred from electronic annotation. Source: Ensembl

establishment of planar polarity

Inferred from mutant phenotype PubMed 18953410. Source: MGI

face development

Inferred from electronic annotation. Source: Ensembl

heart looping

Inferred from genetic interaction PubMed 19795512. Source: MGI

hematopoietic stem cell proliferation

Inferred from direct assay PubMed 9160667. Source: MGI

hindgut morphogenesis

Inferred from mutant phenotype PubMed 18804104. Source: MGI

hypophysis morphogenesis

Inferred from mutant phenotype PubMed 15037319. Source: MGI

inner ear morphogenesis

Inferred from direct assay PubMed 18991062. Source: MGI

keratinocyte differentiation

Inferred from electronic annotation. Source: Ensembl

lateral sprouting involved in mammary gland duct morphogenesis

Inferred from direct assay PubMed 17898001. Source: MGI

limb morphogenesis

Inferred from mutant phenotype PubMed 19795512. Source: MGI

lung development

Inferred from mutant phenotype PubMed 12142021. Source: MGI

male gonad development

Inferred from mutant phenotype PubMed 18804104PubMed 19100252. Source: MGI

mammary gland branching involved in thelarche

Inferred from direct assay PubMed 17898001. Source: MGI

mesenchymal-epithelial cell signaling

Inferred from mutant phenotype PubMed 15073149. Source: MGI

midgut development

Inferred from mutant phenotype PubMed 19100728. Source: MGI

morphogenesis of an epithelium

Inferred from mutant phenotype PubMed 15073149. Source: MGI

negative chemotaxis

Inferred from genetic interaction PubMed 16723543. Source: MGI

negative regulation of BMP signaling pathway

Inferred from mutant phenotype PubMed 18351662. Source: MGI

negative regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of axon extension involved in axon guidance

Inferred from genetic interaction PubMed 16723543. Source: MGI

negative regulation of canonical Wnt signaling pathway

Inferred from direct assay PubMed 18986540. Source: BHF-UCL

negative regulation of epithelial cell proliferation

Inferred from direct assay PubMed 17898001. Source: MGI

negative regulation of fat cell differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of fibroblast growth factor receptor signaling pathway

Inferred from mutant phenotype PubMed 18351662. Source: MGI

negative regulation of mesenchymal cell proliferation

Inferred from electronic annotation. Source: Ensembl

negative regulation of prostatic bud formation

Inferred from mutant phenotype PubMed 18804104. Source: MGI

negative regulation of synapse assembly

Inferred from direct assay PubMed 18986540. Source: BHF-UCL

negative regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

neural tube closure

Inferred from genetic interaction PubMed 17433286PubMed 19795512. Source: MGI

neural tube development

Inferred from genetic interaction PubMed 19795512. Source: MGI

neuron projection morphogenesis

Inferred from direct assay PubMed 21483795. Source: UniProtKB

non-canonical Wnt signaling pathway via JNK cascade

Inferred from mutant phenotype PubMed 20032469. Source: BHF-UCL

olfactory bulb interneuron development

Inferred from mutant phenotype PubMed 21539518. Source: UniProtKB

organ morphogenesis

Traceable author statement PubMed 9889131. Source: MGI

palate development

Inferred from electronic annotation. Source: Ensembl

pericardium morphogenesis

Inferred from genetic interaction PubMed 19795512. Source: MGI

planar cell polarity pathway involved in cardiac muscle tissue morphogenesis

Inferred from mutant phenotype PubMed 19056682. Source: MGI

planar cell polarity pathway involved in cardiac right atrium morphogenesis

Inferred from mutant phenotype PubMed 19056682. Source: MGI

planar cell polarity pathway involved in neural tube closure

Inferred from mutant phenotype PubMed 19056682. Source: MGI

planar cell polarity pathway involved in outflow tract morphogenesis

Inferred from mutant phenotype PubMed 19056682. Source: MGI

planar cell polarity pathway involved in pericardium morphogenesis

Inferred from mutant phenotype PubMed 19056682. Source: MGI

planar cell polarity pathway involved in ventricular septum morphogenesis

Inferred from mutant phenotype PubMed 19056682. Source: MGI

positive regulation of JNK cascade

Inferred from mutant phenotype PubMed 20032469. Source: BHF-UCL

positive regulation of JUN kinase activity

Inferred from direct assay PubMed 19300477. Source: MGI

positive regulation of NF-kappaB transcription factor activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of T cell chemotaxis

Inferred from electronic annotation. Source: Ensembl

positive regulation of angiogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of cGMP metabolic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of cartilage development

Inferred from direct assay PubMed 18991062. Source: MGI

positive regulation of cell proliferation

Inferred from direct assay PubMed 9160667. Source: MGI

positive regulation of cell-cell adhesion mediated by cadherin

Inferred from direct assay PubMed 10893270. Source: MGI

positive regulation of chemokine biosynthetic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytokine secretion involved in immune response

Inferred from electronic annotation. Source: Ensembl

positive regulation of endothelial cell migration

Inferred from electronic annotation. Source: Ensembl

positive regulation of endothelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of epithelial cell proliferation

Inferred from mutant phenotype PubMed 19100728. Source: MGI

positive regulation of fibroblast proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of inflammatory response

Inferred from electronic annotation. Source: Ensembl

positive regulation of interferon-gamma production

Inferred from mutant phenotype PubMed 16601243. Source: BHF-UCL

positive regulation of interleukin-1 beta secretion

Inferred from direct assay PubMed 18174455. Source: BHF-UCL

positive regulation of interleukin-6 production

Inferred from direct assay PubMed 18174455. Source: BHF-UCL

positive regulation of interleukin-8 secretion

Inferred from direct assay PubMed 18174455. Source: BHF-UCL

positive regulation of macrophage activation

Inferred from electronic annotation. Source: Ensembl

positive regulation of macrophage cytokine production

Inferred from electronic annotation. Source: Ensembl

positive regulation of meiosis

Inferred from genetic interaction PubMed 20106871. Source: MGI

positive regulation of mesenchymal cell proliferation

Inferred from mutant phenotype PubMed 19100728. Source: MGI

positive regulation of neuron projection development

Inferred from direct assay PubMed 21539518. Source: UniProtKB

positive regulation of ossification

Inferred from electronic annotation. Source: Ensembl

positive regulation of peptidyl-serine phosphorylation

Inferred from genetic interaction PubMed 17986005. Source: MGI

positive regulation of peptidyl-threonine phosphorylation

Inferred from genetic interaction PubMed 17986005. Source: MGI

positive regulation of protein catabolic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein kinase C signaling

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein phosphorylation

Inferred from direct assay PubMed 15143170. Source: MGI

positive regulation of thymocyte apoptotic process

Inferred from mutant phenotype PubMed 18070933. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 18929644. Source: BHF-UCL

positive regulation of type I interferon-mediated signaling pathway

Inferred from electronic annotation. Source: Ensembl

post-anal tail morphogenesis

Inferred from mutant phenotype PubMed 19795512. Source: MGI

primitive streak formation

Inferred from genetic interaction PubMed 19795512. Source: MGI

protein phosphorylation

Inferred from genetic interaction PubMed 17898001. Source: MGI

regulation of branching involved in mammary gland duct morphogenesis

Inferred from direct assay PubMed 17898001. Source: MGI

signal transduction

Traceable author statement PubMed 9889131. Source: MGI

somitogenesis

Inferred from genetic interaction PubMed 19795512. Source: MGI

tube closure

Inferred from mutant phenotype PubMed 19100728. Source: MGI

type B pancreatic cell development

Inferred from mutant phenotype PubMed 16246260. Source: MGI

urinary bladder development

Inferred from mutant phenotype PubMed 18804104. Source: MGI

uterus development

Inferred from mutant phenotype PubMed 15073149. Source: MGI

vagina development

Inferred from mutant phenotype PubMed 15073149. Source: MGI

wound healing

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell surface

Inferred from direct assay PubMed 8167409. Source: BHF-UCL

endoplasmic reticulum lumen

Traceable author statement. Source: Reactome

extracellular matrix

Inferred from direct assay PubMed 8167409. Source: MGI

extracellular region

Traceable author statement. Source: Reactome

extracellular space

Inferred from Biological aspect of Ancestor. Source: RefGenome

proteinaceous extracellular matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncytokine activity

Inferred from direct assay PubMed 18986540. Source: BHF-UCL

frizzled binding

Inferred from physical interaction PubMed 10557084. Source: BHF-UCL

frizzled-2 binding

Inferred from direct assay PubMed 10893270PubMed 19910923. Source: MGI

protein binding

Inferred from physical interaction Ref.10. Source: UniProtKB

protein domain specific binding

Inferred from physical interaction PubMed 16723543. Source: UniProtKB

receptor binding

Inferred from physical interaction PubMed 16723543. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from sequence or structural similarity. Source: UniProtKB

transcription regulatory region DNA binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

KlO350822EBI-1570983,EBI-1570828
WIF1Q9Y5W57EBI-1570983,EBI-3922719From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P22725-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P22725-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3737 Potential
Propeptide38 – 6124
PRO_0000352797
Chain62 – 380319Protein Wnt-5a
PRO_0000041428

Amino acid modifications

Lipidation1041S-palmitoyl cysteine Ref.9
Lipidation2441O-palmitoyl serine; by PORCN By similarity
Glycosylation1141N-linked (GlcNAc...) Ref.9
Glycosylation1201N-linked (GlcNAc...) Ref.9
Glycosylation3121N-linked (GlcNAc...) Ref.9
Glycosylation3261N-linked (GlcNAc...) Ref.9

Natural variations

Alternative sequence1 – 2020Missing in isoform 2.
VSP_035595

Experimental info

Mutagenesis1041C → A: Abolishes palmitoylation. No effect on secretion. Ref.9
Mutagenesis1141N → Q: Abolishes glycosylation; when associated with Q-120, Q-312 and Q-326. Ref.9
Mutagenesis1201N → Q: Abolishes glycosylation; when associated with Q-114, Q-312 and Q-326. Ref.9
Mutagenesis3121N → Q: Abolishes glycosylation; when associated with Q-114, Q-120 and Q-326. Ref.9
Mutagenesis3261N → Q: Abolishes glycosylation; when associated with Q-114, Q-120 and Q-312. Ref.9
Sequence conflict1681Missing in AAA40567. Ref.1
Sequence conflict190 – 1912YR → HP in AAA40567. Ref.1
Sequence conflict3771F → L in BAC27430. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 4, 2008. Version 2.
Checksum: 64CA36C516D22D8A

FASTA38042,309
        10         20         30         40         50         60 
MKKPIGILSP GVALGTAGGA MSSKFFLMAL ATFFSFAQVV IEANSWWSLG MNNPVQMSEV 

        70         80         90        100        110        120 
YIIGAQPLCS QLAGLSQGQK KLCHLYQDHM QYIGEGAKTG IKECQYQFRH RRWNCSTVDN 

       130        140        150        160        170        180 
TSVFGRVMQI GSRETAFTYA VSAAGVVNAM SRACREGELS TCGCSRAARP KDLPRDWLWG 

       190        200        210        220        230        240 
GCGDNIDYGY RFAKEFVDAR ERERIHAKGS YESARILMNL HNNEAGRRTV YNLADVACKC 

       250        260        270        280        290        300 
HGVSGSCSLK TCWLQLADFR KVGDALKEKY DSAAAMRLNS RGKLVQVNSR FNSPTTQDLV 

       310        320        330        340        350        360 
YIDPSPDYCV RNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV QTERCHCKFH 

       370        380 
WCCYVKCKKC TEIVDQFVCK 

« Hide

Isoform 2 [UniParc].

Checksum: 626372A278164C18
Show »

FASTA36040,489

References

« Hide 'large scale' references
[1]"Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development."
Gavin B.J., McMahon J.A., McMahon A.P.
Genes Dev. 4:2319-2332(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Embryo.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Testis and Vagina.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Mammary tumor.
[5]"Purified Wnt5a protein activates or inhibits beta-catenin-TCF signaling depending on receptor context."
Mikels A.J., Nusse R.
PLoS Biol. 4:E115-E115(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 62-82, FUNCTION.
[6]"A Wnt5a pathway underlies outgrowth of multiple structures in the vertebrate embryo."
Yamaguchi T.P., Bradley A., McMahon A.P., Jones S.
Development 126:1211-1223(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[7]"The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family."
Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T.
Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PORCN.
[8]"Wnt-5a inhibits the canonical Wnt pathway by promoting GSK-3-independent beta-catenin degradation."
Topol L., Jiang X., Choi H., Garrett-Beal L., Carolan P.J., Yang Y.
J. Cell Biol. 162:899-908(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[9]"Post-translational palmitoylation and glycosylation of Wnt-5a are necessary for its signalling."
Kurayoshi M., Yamamoto H., Izumi S., Kikuchi A.
Biochem. J. 402:515-523(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PALMITOYLATION AT CYS-104, GLYCOSYLATION AT ASN-114; ASN-120; ASN-312 AND ASN-326, MUTAGENESIS OF CYS-104; ASN-114; ASN-120; ASN-312 AND ASN-326.
[10]"Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation."
Fu J., Jiang M., Mirando A.J., Yu H.-M., Hsu W.
Proc. Natl. Acad. Sci. U.S.A. 106:18598-18603(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH WLS.
[11]"Tiki1 is required for head formation via Wnt cleavage-oxidation and inactivation."
Zhang X., Abreu J.G., Yokota C., Macdonald B.T., Singh S., Coburn K.L., Cheong S.M., Zhang M.M., Ye Q.Z., Hang H.C., Steen H., He X.
Cell 149:1565-1577(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING BY TIKI1 AND TIKI2, DISULFIDE BONDS, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M89798 mRNA. Translation: AAA40567.1.
AK031512 mRNA. Translation: BAC27430.1.
AK031597 mRNA. Translation: BAC27468.1.
AK036824 mRNA. Translation: BAC29593.1.
CT025649 Genomic DNA. Translation: CAM27826.1.
CT025649 Genomic DNA. Translation: CAM27827.1.
BC018425 mRNA. Translation: AAH18425.1.
CCDSCCDS26888.1. [P22725-1]
CCDS56946.1. [P22725-2]
PIRD36470.
RefSeqNP_001243153.1. NM_001256224.1. [P22725-2]
NP_033550.2. NM_009524.3. [P22725-1]
XP_006518987.1. XM_006518924.1. [P22725-2]
XP_006518988.1. XM_006518925.1. [P22725-2]
UniGeneMm.287544.

3D structure databases

ProteinModelPortalP22725.
SMRP22725. Positions 94-309.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204577. 7 interactions.
DIPDIP-39893N.
IntActP22725. 4 interactions.
MINTMINT-6476731.

Proteomic databases

PRIDEP22725.

Protocols and materials databases

DNASU22418.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000063465; ENSMUSP00000064878; ENSMUSG00000021994. [P22725-1]
ENSMUST00000112272; ENSMUSP00000107891; ENSMUSG00000021994. [P22725-2]
GeneID22418.
KEGGmmu:22418.
UCSCuc007sug.2. mouse. [P22725-1]

Organism-specific databases

CTD7474.
MGIMGI:98958. Wnt5a.

Phylogenomic databases

eggNOGNOG284879.
GeneTreeENSGT00740000115016.
HOGENOMHOG000039529.
HOVERGENHBG001595.
InParanoidP22725.
KOK00444.
OMAIVERCHC.
OrthoDBEOG7C8GJ8.
PhylomeDBP22725.
TreeFamTF105310.

Enzyme and pathway databases

ReactomeREACT_188257. Signal Transduction.

Gene expression databases

BgeeP22725.
CleanExMM_WNT5A.
GenevestigatorP22725.

Family and domain databases

InterProIPR005817. Wnt.
IPR026538. Wnt5a.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF33. PTHR12027:SF33. 1 hit.
PfamPF00110. wnt. 1 hit.
[Graphical view]
PRINTSPR01349. WNTPROTEIN.
SMARTSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302841.
PROP22725.
SOURCESearch...

Entry information

Entry nameWNT5A_MOUSE
AccessionPrimary (citable) accession number: P22725
Secondary accession number(s): Q8BM17, Q8BMF9, Q8VCV6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 4, 2008
Last modified: July 9, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot