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Protein

Protein Wnt-5a

Gene

Wnt5a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes.4 Publications

GO - Molecular functioni

  • cytokine activity Source: BHF-UCL
  • frizzled-2 binding Source: MGI
  • frizzled binding Source: BHF-UCL
  • protein domain specific binding Source: UniProtKB
  • receptor binding Source: MGI
  • receptor tyrosine kinase-like orphan receptor binding Source: MGI
  • sequence-specific DNA binding transcription factor activity Source: UniProtKB
  • transcription regulatory region DNA binding Source: MGI

GO - Biological processi

  • activation of JUN kinase activity Source: MGI
  • activation of MAPK activity Source: MGI
  • activation of protein kinase B activity Source: MGI
  • ameboidal-type cell migration Source: MGI
  • anterior/posterior axis specification, embryo Source: BHF-UCL
  • anterior/posterior pattern specification Source: MGI
  • axis elongation Source: MGI
  • axon guidance Source: UniProtKB
  • canonical Wnt signaling pathway Source: BHF-UCL
  • cartilage development Source: UniProtKB-KW
  • cell-cell signaling Source: MGI
  • cell fate commitment Source: GO_Central
  • cell migration Source: MGI
  • cellular protein localization Source: MGI
  • cellular response to calcium ion Source: Ensembl
  • cellular response to interferon-gamma Source: Ensembl
  • cellular response to lipopolysaccharide Source: Ensembl
  • cellular response to molecule of bacterial origin Source: BHF-UCL
  • cellular response to transforming growth factor beta stimulus Source: Ensembl
  • cervix development Source: MGI
  • cochlea morphogenesis Source: MGI
  • convergent extension Source: MGI
  • convergent extension involved in organogenesis Source: MGI
  • determination of left/right symmetry Source: MGI
  • development of primary male sexual characteristics Source: MGI
  • digestive tract morphogenesis Source: MGI
  • dopaminergic neuron differentiation Source: CACAO
  • embryonic digit morphogenesis Source: MGI
  • embryonic limb morphogenesis Source: MGI
  • embryonic skeletal system development Source: MGI
  • epithelial cell proliferation involved in mammary gland duct elongation Source: MGI
  • epithelial to mesenchymal transition Source: Ensembl
  • establishment of planar polarity Source: MGI
  • face development Source: MGI
  • genitalia development Source: MGI
  • heart looping Source: MGI
  • hematopoietic stem cell proliferation Source: MGI
  • hindgut morphogenesis Source: MGI
  • hypophysis morphogenesis Source: MGI
  • inner ear morphogenesis Source: MGI
  • JNK cascade Source: MGI
  • keratinocyte differentiation Source: Ensembl
  • lateral sprouting involved in mammary gland duct morphogenesis Source: MGI
  • limb morphogenesis Source: MGI
  • lung development Source: MGI
  • male gonad development Source: MGI
  • mammary gland branching involved in thelarche Source: MGI
  • melanocyte proliferation Source: CACAO
  • mesenchymal-epithelial cell signaling Source: MGI
  • midbrain development Source: CACAO
  • midgut development Source: MGI
  • morphogenesis of an epithelium Source: MGI
  • negative chemotaxis Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of axon extension involved in axon guidance Source: MGI
  • negative regulation of BMP signaling pathway Source: MGI
  • negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
  • negative regulation of epithelial cell proliferation Source: MGI
  • negative regulation of fat cell differentiation Source: MGI
  • negative regulation of fibroblast growth factor receptor signaling pathway Source: MGI
  • negative regulation of melanin biosynthetic process Source: CACAO
  • negative regulation of mesenchymal cell proliferation Source: MGI
  • negative regulation of prostatic bud formation Source: MGI
  • negative regulation of synapse assembly Source: BHF-UCL
  • negative regulation of transcription, DNA-templated Source: MGI
  • neural tube closure Source: MGI
  • neural tube development Source: MGI
  • neuron projection morphogenesis Source: UniProtKB
  • non-canonical Wnt signaling pathway Source: MGI
  • non-canonical Wnt signaling pathway via JNK cascade Source: BHF-UCL
  • olfactory bulb interneuron development Source: UniProtKB
  • organ morphogenesis Source: MGI
  • palate development Source: MGI
  • pericardium morphogenesis Source: MGI
  • planar cell polarity pathway involved in cardiac muscle tissue morphogenesis Source: MGI
  • planar cell polarity pathway involved in cardiac right atrium morphogenesis Source: MGI
  • planar cell polarity pathway involved in neural tube closure Source: MGI
  • planar cell polarity pathway involved in outflow tract morphogenesis Source: MGI
  • planar cell polarity pathway involved in pericardium morphogenesis Source: MGI
  • planar cell polarity pathway involved in ventricular septum morphogenesis Source: MGI
  • positive regulation of angiogenesis Source: MGI
  • positive regulation of cartilage development Source: MGI
  • positive regulation of cell-cell adhesion mediated by cadherin Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of cGMP metabolic process Source: MGI
  • positive regulation of chemokine biosynthetic process Source: MGI
  • positive regulation of cytokine production Source: MGI
  • positive regulation of cytokine secretion involved in immune response Source: MGI
  • positive regulation of endocytosis Source: MGI
  • positive regulation of endothelial cell migration Source: MGI
  • positive regulation of endothelial cell proliferation Source: MGI
  • positive regulation of epithelial cell proliferation Source: MGI
  • positive regulation of fibroblast proliferation Source: MGI
  • positive regulation of inflammatory response Source: MGI
  • positive regulation of interferon-gamma production Source: BHF-UCL
  • positive regulation of interleukin-1 beta secretion Source: BHF-UCL
  • positive regulation of interleukin-6 production Source: BHF-UCL
  • positive regulation of interleukin-8 secretion Source: BHF-UCL
  • positive regulation of JNK cascade Source: BHF-UCL
  • positive regulation of JUN kinase activity Source: MGI
  • positive regulation of macrophage activation Source: MGI
  • positive regulation of macrophage cytokine production Source: MGI
  • positive regulation of meiotic nuclear division Source: MGI
  • positive regulation of mesenchymal cell proliferation Source: MGI
  • positive regulation of neuron projection development Source: UniProtKB
  • positive regulation of NF-kappaB transcription factor activity Source: MGI
  • positive regulation of non-canonical Wnt signaling pathway Source: CACAO
  • positive regulation of ossification Source: MGI
  • positive regulation of peptidyl-serine phosphorylation Source: MGI
  • positive regulation of peptidyl-threonine phosphorylation Source: MGI
  • positive regulation of protein catabolic process Source: MGI
  • positive regulation of protein kinase C activity Source: MGI
  • positive regulation of protein kinase C signaling Source: MGI
  • positive regulation of protein phosphorylation Source: MGI
  • positive regulation of response to cytokine stimulus Source: MGI
  • positive regulation of T cell chemotaxis Source: MGI
  • positive regulation of thymocyte apoptotic process Source: MGI
  • positive regulation of transcription, DNA-templated Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of type I interferon-mediated signaling pathway Source: MGI
  • post-anal tail morphogenesis Source: MGI
  • primitive streak formation Source: MGI
  • protein phosphorylation Source: MGI
  • regulation of branching involved in mammary gland duct morphogenesis Source: MGI
  • regulation of protein localization Source: MGI
  • signal transduction Source: MGI
  • somitogenesis Source: MGI
  • tube closure Source: MGI
  • type B pancreatic cell development Source: MGI
  • urinary bladder development Source: MGI
  • uterus development Source: MGI
  • vagina development Source: MGI
  • Wnt signaling pathway Source: MGI
  • Wnt signaling pathway, calcium modulating pathway Source: BHF-UCL
  • Wnt signaling pathway, planar cell polarity pathway Source: MGI
  • wound healing Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Chondrogenesis, Differentiation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiREACT_295052. WNT ligand biogenesis and trafficking.
REACT_299462. WNT5A-dependent internalization of FZD4.
REACT_305098. negative regulation of TCF-dependent signaling by WNT ligand antagonists.
REACT_330043. WNT5A-dependent internalization of FZD2, FZD5 and ROR2.
REACT_331940. Ca2+ pathway.
REACT_333985. PCP/CE pathway.
REACT_335449. Class B/2 (Secretin family receptors).
REACT_342636. Asymmetric localization of PCP proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-5a
Gene namesi
Name:Wnt5a
Synonyms:Wnt-5a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:98958. Wnt5a.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: BHF-UCL
  • endoplasmic reticulum lumen Source: Reactome
  • extracellular region Source: Reactome
  • extracellular space Source: MGI
  • proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Disruption phenotypei

Mice display perinatal lethality and display gross morphological defects in outgrowing tissues. They are truncated caudally, displaying loss of the tail and a significant shortening of the anterior-posterior axis. The shape of the head is abnormal with truncated snout, mandible and tongue and reduced outgrowth of the external ear. Fore- and hindlimbs lack digits, the genital tubercle is missing and chondrocyte differentiation is inhibited.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi104 – 1041C → A: No effect on secretion. 1 Publication
Mutagenesisi114 – 1141N → Q: Abolishes glycosylation; when associated with Q-120, Q-312 and Q-326. 1 Publication
Mutagenesisi120 – 1201N → Q: Abolishes glycosylation; when associated with Q-114, Q-312 and Q-326. 1 Publication
Mutagenesisi312 – 3121N → Q: Abolishes glycosylation; when associated with Q-114, Q-120 and Q-326. 1 Publication
Mutagenesisi326 – 3261N → Q: Abolishes glycosylation; when associated with Q-114, Q-120 and Q-312. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3737Sequence AnalysisAdd
BLAST
Propeptidei38 – 61241 PublicationPRO_0000352797Add
BLAST
Chaini62 – 380319Protein Wnt-5aPRO_0000041428Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi104 ↔ 115By similarity
Glycosylationi114 – 1141N-linked (GlcNAc...)1 Publication
Glycosylationi120 – 1201N-linked (GlcNAc...)1 Publication
Disulfide bondi154 ↔ 162By similarity
Disulfide bondi164 ↔ 182By similarity
Disulfide bondi238 ↔ 252By similarity
Disulfide bondi240 ↔ 247By similarity
Lipidationi244 – 2441O-palmitoleyl serine; by PORCNBy similarity
Glycosylationi312 – 3121N-linked (GlcNAc...)1 Publication
Disulfide bondi325 ↔ 340By similarity
Glycosylationi326 – 3261N-linked (GlcNAc...)1 Publication
Disulfide bondi355 ↔ 370By similarity
Disulfide bondi357 ↔ 367By similarity
Disulfide bondi362 ↔ 363By similarity

Post-translational modificationi

Glycosylation is necessary for secretion but not for activity.1 Publication
Palmitoleylation is required for efficient binding to frizzled receptors. Depalmitoleylation leads to Wnt signaling pathway inhibition.By similarity
Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT5A.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

PRIDEiP22725.

Expressioni

Tissue specificityi

Expressed in a gradient at the caudal end of the embryo during gastrulation and later in the distal-most aspect of several structures that extend from the body such as the limbs and genital tubercle.1 Publication

Gene expression databases

BgeeiP22725.
CleanExiMM_WNT5A.
GenevisibleiP22725. MM.

Interactioni

Subunit structurei

Homooligomer; disulfide-linked, leading to inactivation. Interacts with PORCN. Interacts with WLS.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KlO350822EBI-1570983,EBI-1570828
WIF1Q9Y5W57EBI-1570983,EBI-3922719From a different organism.

Protein-protein interaction databases

BioGridi204577. 7 interactions.
DIPiDIP-39893N.
IntActiP22725. 4 interactions.
MINTiMINT-6476731.
STRINGi10090.ENSMUSP00000064878.

Structurei

3D structure databases

ProteinModelPortaliP22725.
SMRiP22725. Positions 94-309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG284879.
GeneTreeiENSGT00760000118943.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiP22725.
KOiK00444.
OMAiIVERCHC.
OrthoDBiEOG7C8GJ8.
PhylomeDBiP22725.
TreeFamiTF105310.

Family and domain databases

InterProiIPR005817. Wnt.
IPR026538. Wnt5a.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF33. PTHR12027:SF33. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P22725-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKKPIGILSP GVALGTAGGA MSSKFFLMAL ATFFSFAQVV IEANSWWSLG
60 70 80 90 100
MNNPVQMSEV YIIGAQPLCS QLAGLSQGQK KLCHLYQDHM QYIGEGAKTG
110 120 130 140 150
IKECQYQFRH RRWNCSTVDN TSVFGRVMQI GSRETAFTYA VSAAGVVNAM
160 170 180 190 200
SRACREGELS TCGCSRAARP KDLPRDWLWG GCGDNIDYGY RFAKEFVDAR
210 220 230 240 250
ERERIHAKGS YESARILMNL HNNEAGRRTV YNLADVACKC HGVSGSCSLK
260 270 280 290 300
TCWLQLADFR KVGDALKEKY DSAAAMRLNS RGKLVQVNSR FNSPTTQDLV
310 320 330 340 350
YIDPSPDYCV RNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV
360 370 380
QTERCHCKFH WCCYVKCKKC TEIVDQFVCK
Length:380
Mass (Da):42,309
Last modified:November 4, 2008 - v2
Checksum:i64CA36C516D22D8A
GO
Isoform 2 (identifier: P22725-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Note: No experimental confirmation available.
Show »
Length:360
Mass (Da):40,489
Checksum:i626372A278164C18
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti168 – 1681Missing in AAA40567 (PubMed:2279700).Curated
Sequence conflicti190 – 1912YR → HP in AAA40567 (PubMed:2279700).Curated
Sequence conflicti377 – 3771F → L in BAC27430 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2020Missing in isoform 2. 1 PublicationVSP_035595Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89798 mRNA. Translation: AAA40567.1.
AK031512 mRNA. Translation: BAC27430.1.
AK031597 mRNA. Translation: BAC27468.1.
AK036824 mRNA. Translation: BAC29593.1.
CT025649 Genomic DNA. Translation: CAM27826.1.
CT025649 Genomic DNA. Translation: CAM27827.1.
BC018425 mRNA. Translation: AAH18425.1.
CCDSiCCDS26888.1. [P22725-1]
CCDS56946.1. [P22725-2]
PIRiD36470.
RefSeqiNP_001243153.1. NM_001256224.1. [P22725-2]
NP_033550.2. NM_009524.3. [P22725-1]
XP_006518987.1. XM_006518924.2. [P22725-2]
XP_006518988.1. XM_006518925.2. [P22725-2]
UniGeneiMm.287544.

Genome annotation databases

EnsembliENSMUST00000063465; ENSMUSP00000064878; ENSMUSG00000021994. [P22725-1]
ENSMUST00000112272; ENSMUSP00000107891; ENSMUSG00000021994. [P22725-2]
GeneIDi22418.
KEGGimmu:22418.
UCSCiuc007sug.2. mouse. [P22725-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89798 mRNA. Translation: AAA40567.1.
AK031512 mRNA. Translation: BAC27430.1.
AK031597 mRNA. Translation: BAC27468.1.
AK036824 mRNA. Translation: BAC29593.1.
CT025649 Genomic DNA. Translation: CAM27826.1.
CT025649 Genomic DNA. Translation: CAM27827.1.
BC018425 mRNA. Translation: AAH18425.1.
CCDSiCCDS26888.1. [P22725-1]
CCDS56946.1. [P22725-2]
PIRiD36470.
RefSeqiNP_001243153.1. NM_001256224.1. [P22725-2]
NP_033550.2. NM_009524.3. [P22725-1]
XP_006518987.1. XM_006518924.2. [P22725-2]
XP_006518988.1. XM_006518925.2. [P22725-2]
UniGeneiMm.287544.

3D structure databases

ProteinModelPortaliP22725.
SMRiP22725. Positions 94-309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204577. 7 interactions.
DIPiDIP-39893N.
IntActiP22725. 4 interactions.
MINTiMINT-6476731.
STRINGi10090.ENSMUSP00000064878.

Proteomic databases

PRIDEiP22725.

Protocols and materials databases

DNASUi22418.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063465; ENSMUSP00000064878; ENSMUSG00000021994. [P22725-1]
ENSMUST00000112272; ENSMUSP00000107891; ENSMUSG00000021994. [P22725-2]
GeneIDi22418.
KEGGimmu:22418.
UCSCiuc007sug.2. mouse. [P22725-1]

Organism-specific databases

CTDi7474.
MGIiMGI:98958. Wnt5a.

Phylogenomic databases

eggNOGiNOG284879.
GeneTreeiENSGT00760000118943.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiP22725.
KOiK00444.
OMAiIVERCHC.
OrthoDBiEOG7C8GJ8.
PhylomeDBiP22725.
TreeFamiTF105310.

Enzyme and pathway databases

ReactomeiREACT_295052. WNT ligand biogenesis and trafficking.
REACT_299462. WNT5A-dependent internalization of FZD4.
REACT_305098. negative regulation of TCF-dependent signaling by WNT ligand antagonists.
REACT_330043. WNT5A-dependent internalization of FZD2, FZD5 and ROR2.
REACT_331940. Ca2+ pathway.
REACT_333985. PCP/CE pathway.
REACT_335449. Class B/2 (Secretin family receptors).
REACT_342636. Asymmetric localization of PCP proteins.

Miscellaneous databases

ChiTaRSiWnt5a. mouse.
NextBioi302841.
PROiP22725.
SOURCEiSearch...

Gene expression databases

BgeeiP22725.
CleanExiMM_WNT5A.
GenevisibleiP22725. MM.

Family and domain databases

InterProiIPR005817. Wnt.
IPR026538. Wnt5a.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF33. PTHR12027:SF33. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development."
    Gavin B.J., McMahon J.A., McMahon A.P.
    Genes Dev. 4:2319-2332(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Embryo.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Testis and Vagina.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Mammary tumor.
  5. "Purified Wnt5a protein activates or inhibits beta-catenin-TCF signaling depending on receptor context."
    Mikels A.J., Nusse R.
    PLoS Biol. 4:E115-E115(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 62-82, FUNCTION.
  6. "A Wnt5a pathway underlies outgrowth of multiple structures in the vertebrate embryo."
    Yamaguchi T.P., Bradley A., McMahon A.P., Jones S.
    Development 126:1211-1223(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  7. "The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family."
    Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T.
    Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PORCN.
  8. "Wnt-5a inhibits the canonical Wnt pathway by promoting GSK-3-independent beta-catenin degradation."
    Topol L., Jiang X., Choi H., Garrett-Beal L., Carolan P.J., Yang Y.
    J. Cell Biol. 162:899-908(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  9. "Post-translational palmitoylation and glycosylation of Wnt-5a are necessary for its signalling."
    Kurayoshi M., Yamamoto H., Izumi S., Kikuchi A.
    Biochem. J. 402:515-523(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PRELIMINARY CYSTEINE PALMITOYLATION, GLYCOSYLATION AT ASN-114; ASN-120; ASN-312 AND ASN-326, MUTAGENESIS OF CYS-104; ASN-114; ASN-120; ASN-312 AND ASN-326.
  10. "Reciprocal regulation of Wnt and Gpr177/mouse Wntless is required for embryonic axis formation."
    Fu J., Jiang M., Mirando A.J., Yu H.-M., Hsu W.
    Proc. Natl. Acad. Sci. U.S.A. 106:18598-18603(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH WLS.
  11. "Tiki1 is required for head formation via Wnt cleavage-oxidation and inactivation."
    Zhang X., Abreu J.G., Yokota C., Macdonald B.T., Singh S., Coburn K.L., Cheong S.M., Zhang M.M., Ye Q.Z., Hang H.C., Steen H., He X.
    Cell 149:1565-1577(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEOLYTIC PROCESSING BY TIKI1 AND TIKI2, DISULFIDE BONDS, SUBUNIT.

Entry informationi

Entry nameiWNT5A_MOUSE
AccessioniPrimary (citable) accession number: P22725
Secondary accession number(s): Q8BM17, Q8BMF9, Q8VCV6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: November 4, 2008
Last modified: June 24, 2015
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

A palmitoylation site was proposed at Cys-104, but it was later shown that this cysteine is engaged in a disulfide bond.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.