P22724 (WNT4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-4

Gene names

Name:	Wnt4
Synonyms:	Wnt-4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. May be an intracellular signaling molecule involved in segmentation of the forebrain. Is likely to signal over only few cell diameters By similarity. Seems to be involved in kidney development.
Subunit structure	Interacts with PORCN. Ref.2
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	In adults in lung and brain.
Post-translational modification	Palmitoylation at Ser-212 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-78. Palmitoylation is necessary for proper trafficking to cell surface By similarity.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adrenal gland development Inferred from electronic annotation. Source: Compara androgen biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB anterior/posterior pattern specification Inferred from Biological aspect of Ancestor. Source: RefGenome axis specification Inferred from Biological aspect of Ancestor. Source: RefGenome branching involved in ureteric bud morphogenesis Inferred from mutant phenotype PubMed 16054034. Source: MGI canonical Wnt receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB cellular response to transforming growth factor beta stimulus Inferred from electronic annotation. Source: Compara embryonic epithelial tube formation Inferred from direct assay PubMed 16546160. Source: MGI endoderm development Inferred from Biological aspect of Ancestor. Source: RefGenome epithelial to mesenchymal transition Inferred from electronic annotation. Source: Compara establishment of protein localization to plasma membrane Inferred from mutant phenotype PubMed 17976063. Source: UniProtKB female gonad development Inferred from mutant phenotype PubMed 17976063. Source: UniProtKB female sex determination Inferred from sequence or structural similarity PubMed 11283799. Source: UniProtKB immature T cell proliferation in thymus Inferred from mutant phenotype PubMed 18617424. Source: MGI liver development Inferred from electronic annotation. Source: Compara male gonad development Inferred from sequence or structural similarity PubMed 11283799. Source: UniProtKB mesenchymal to epithelial transition Inferred from mutant phenotype PubMed 7990960. Source: MGI mesonephric tubule development Inferred from genetic interaction PubMed 20040500. Source: MGI metanephric mesenchymal cell differentiation Non-traceable author statement PubMed 16368682. Source: UniProtKB metanephric nephron morphogenesis Inferred from mutant phenotype PubMed 19830824. Source: MGI negative regulation of apoptotic process Inferred from genetic interaction PubMed 20454446. Source: MGI negative regulation of canonical Wnt receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell differentiation Inferred from mutant phenotype PubMed 15581876. Source: MGI negative regulation of cell migration Inferred from genetic interaction PubMed 23027131. Source: BHF-UCL negative regulation of fibroblast growth factor receptor signaling pathway Inferred from genetic interaction PubMed 16700629. Source: MGI negative regulation of male gonad development Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of testicular blood vessel morphogenesis Inferred from electronic annotation. Source: Compara negative regulation of testosterone biosynthetic process Inferred from mutant phenotype PubMed 12949260. Source: UniProtKB negative regulation of transcription from RNA polymerase II promoter Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of wound healing Inferred from genetic interaction PubMed 23027131. Source: BHF-UCL non-canonical Wnt receptor signaling pathway Inferred from direct assay PubMed 18617424. Source: MGI non-canonical Wnt receptor signaling pathway via MAPK cascade Inferred from electronic annotation. Source: Compara oocyte development Inferred from mutant phenotype PubMed 9989404. Source: MGI paramesonephric duct development Inferred from mutant phenotype PubMed 14695376. Source: UniProtKB positive regulation of Rho GTPase activity Inferred from genetic interaction PubMed 23027131. Source: BHF-UCL positive regulation of aldosterone biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of bone mineralization Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of canonical Wnt receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of collagen biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cortisol biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of dermatome development Inferred from sequence or structural similarity PubMed 10654605. Source: BHF-UCL positive regulation of focal adhesion assembly Inferred from genetic interaction PubMed 23027131. Source: BHF-UCL positive regulation of meiosis Inferred from genetic interaction PubMed 20106871. Source: MGI positive regulation of osteoblast differentiation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of stress fiber assembly Inferred from genetic interaction PubMed 23027131. Source: BHF-UCL positive regulation of transcription, DNA-dependent Inferred from direct assay PubMed 11283799. Source: UniProtKB protein palmitoylation Inferred from sequence or structural similarity. Source: UniProtKB regulation of cell-cell adhesion Inferred from mutant phenotype PubMed 20106871. Source: MGI renal vesicle formation Inferred from mutant phenotype PubMed 16054034. Source: MGI renal vesicle induction Inferred from direct assay PubMed 11507767. Source: MGI smooth muscle cell differentiation Inferred from direct assay PubMed 16546160. Source: MGI somatotropin secreting cell differentiation Inferred from mutant phenotype PubMed 18351662. Source: MGI tertiary branching involved in mammary gland duct morphogenesis Inferred from mutant phenotype PubMed 10733525. Source: MGI thyroid-stimulating hormone-secreting cell differentiation Inferred from mutant phenotype PubMed 18351662. Source: MGI
Cellular_component	Golgi apparatus Inferred from Biological aspect of Ancestor. Source: RefGenome cell surface Inferred from direct assay PubMed 8167409. Source: BHF-UCL extracellular matrix Inferred from direct assay PubMed 8167409. Source: MGI extracellular space Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	receptor binding Traceable author statement PubMed 9889131. Source: MGI transcription corepressor activity Inferred from direct assay PubMed 12949260. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Kl	O35082	2	EBI-1570945,EBI-1570828

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 351	329	Protein Wnt-4		PRO_0000041422
Amino acid modifications
	Lipidation	78	1	S-palmitoyl cysteine By similarity
	Lipidation	212	1	O-palmitoyl serine; by PORCN By similarity
	Glycosylation	88	1	N-linked (GlcNAc...) Potential
	Glycosylation	297	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P22724 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 7E1C5C739BE939D9	FASTA	351	39,050
	10 20 30 40 50 60 MSPRSCLRSL RLLVFAVFSA AASNWLYLAK LSSVGSISEE ETCEKLKGLI QRQVQMCKRN 70 80 90 100 110 120 LEVMDSVRRG AQLAIEECQY QFRNRRWNCS TLDSLPVFGK VVTQGTREAA FVYAISSAGV 130 140 150 160 170 180 AFAVTRACSS GELEKCGCDR TVHGVSPQGF QWSGCSDNIA YGVAFSQSFV DVRERSKGAS 190 200 210 220 230 240 SSRALMNLHN NEAGRKAILT HMRVECKCHG VSGSCEVKTC WRAVPPFRQV GHALKEKFDG 250 260 270 280 290 300 ATEVEPRRVG SSRALVPRNA QFKPHTDEDL VYLEPSPDFC EQDIRSGVLG TRGRTCNKTS 310 320 330 340 350 KAIDGCELLC CGRGFHTAQV ELAERCGCRF HWCCFVKCRQ CQRLVEMHTC R

References

[1]	"Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development." Gavin B.J., McMahon J.A., McMahon A.P. Genes Dev. 4:2319-2332(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family." Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T. Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PORCN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M89797 mRNA. Translation: AAA40566.1.
IPI	IPI00131373.
PIR	C36470.
RefSeq	NP_033549.1. NM_009523.2.
UniGene	Mm.20355.
3D structure databases
ProteinModelPortal	P22724.
SMR	P22724. Positions 66-350.
ModBase	Search...
Protein-protein interaction databases
IntAct	P22724. 1 interaction.
STRING	10090.ENSMUSP00000036580.
PTM databases
PhosphoSite	P22724.
Proteomic databases
PRIDE	P22724.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000045747; ENSMUSP00000036580; ENSMUSG00000036856.
GeneID	22417.
KEGG	mmu:22417.
Organism-specific databases
CTD	54361.
MGI	MGI:98957. Wnt4.
Phylogenomic databases
eggNOG	NOG284879.
HOGENOM	HOG000039529.
HOVERGEN	HBG001595.
InParanoid	P22724.
KO	K00408.
OMA	QVQICKR.
OrthoDB	EOG49P9ZP.
Gene expression databases
ArrayExpress	P22724.
Bgee	P22724.
CleanEx	MM_WNT4.
Genevestigator	P22724.
GermOnline	ENSMUSG00000036856. Mus musculus.
Family and domain databases
InterPro	IPR005817. Wnt. IPR009142. Wnt4. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF19. PTHR12027:SF19. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01844. WNT4PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302837.
SOURCE	Search...

Entry information

Entry name

WNT4_MOUSE

Accession

Primary (citable) accession number: P22724

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program