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P22724 (WNT4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein Wnt-4
Gene names
Name:Wnt4
Synonyms:Wnt-4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ligand for members of the frizzled family of seven transmembrane receptors. May be an intracellular signaling molecule involved in segmentation of the forebrain. Is likely to signal over only few cell diameters By similarity. Seems to be involved in kidney development.

Subunit structure

Interacts with PORCN. Ref.2

Subcellular location

Secretedextracellular spaceextracellular matrix.

Tissue specificity

In adults in lung and brain.

Post-translational modification

Palmitoylation at Ser-212 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-78. Palmitoylation is necessary for proper trafficking to cell surface By similarity.

Sequence similarities

Belongs to the Wnt family.

Ontologies

Keywords
   Biological processWnt signaling pathway
   Cellular componentExtracellular matrix
Secreted
   DomainSignal
   Molecular functionDevelopmental protein
   PTMGlycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processT cell differentiation in thymus

Inferred from direct assay PubMed 11265645. Source: MGI

adrenal gland development

Inferred from electronic annotation. Source: Ensembl

anatomical structure development

Inferred from genetic interaction PubMed 16818445. Source: MGI

androgen biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

branching involved in ureteric bud morphogenesis

Inferred from mutant phenotype PubMed 16054034. Source: MGI

branching morphogenesis of an epithelial tube

Inferred from genetic interaction PubMed 11948913. Source: MGI

canonical Wnt signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

cell differentiation

Inferred from mutant phenotype PubMed 15581876. Source: MGI

cell fate commitment

Inferred from mutant phenotype PubMed 18351662. Source: MGI

cell-cell signaling

Traceable author statement PubMed 9889131. Source: MGI

cellular response to transforming growth factor beta stimulus

Inferred from electronic annotation. Source: Ensembl

embryonic epithelial tube formation

Inferred from direct assay PubMed 16546160. Source: MGI

epithelial to mesenchymal transition

Inferred from electronic annotation. Source: Ensembl

establishment of protein localization to plasma membrane

Inferred from mutant phenotype PubMed 17976063. Source: UniProtKB

female gonad development

Inferred from mutant phenotype PubMed 17976063. Source: UniProtKB

female sex determination

Inferred from sequence or structural similarity PubMed 11283799. Source: UniProtKB

gamete generation

Inferred from genetic interaction PubMed 15162500. Source: MGI

hormone metabolic process

Inferred from mutant phenotype PubMed 12399432. Source: MGI

immature T cell proliferation in thymus

Inferred from mutant phenotype PubMed 18617424. Source: MGI

kidney morphogenesis

Inferred from genetic interaction PubMed 23362348. Source: MGI

liver development

Inferred from electronic annotation. Source: Ensembl

male gonad development

Inferred from sequence or structural similarity PubMed 11283799. Source: UniProtKB

mesenchymal to epithelial transition

Inferred from mutant phenotype PubMed 7990960. Source: MGI

mesonephric tubule development

Inferred from genetic interaction PubMed 20040500. Source: MGI

mesonephros development

Inferred from mutant phenotype PubMed 14695376. Source: UniProtKB

metanephric mesenchymal cell differentiation

Non-traceable author statement PubMed 16368682. Source: UniProtKB

metanephric nephron development

Inferred from mutant phenotype PubMed 18346943. Source: MGI

metanephric nephron morphogenesis

Inferred from mutant phenotype PubMed 19830824. Source: MGI

metanephric tubule formation

Inferred from mutant phenotype PubMed 21350016. Source: MGI

metanephros development

Inferred from mutant phenotype PubMed 7990960. Source: MGI

negative regulation of apoptotic signaling pathway

Inferred from genetic interaction PubMed 20454446. Source: MGI

negative regulation of canonical Wnt signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cell differentiation

Inferred from mutant phenotype PubMed 15581876. Source: MGI

negative regulation of cell migration

Inferred from genetic interaction PubMed 23027131. Source: BHF-UCL

negative regulation of fibroblast growth factor receptor signaling pathway

Inferred from genetic interaction PubMed 16700629. Source: MGI

negative regulation of gene expression

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of male gonad development

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of steroid biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of testicular blood vessel morphogenesis

Inferred from electronic annotation. Source: Ensembl

negative regulation of testosterone biosynthetic process

Inferred from mutant phenotype PubMed 12949260. Source: UniProtKB

negative regulation of transcription, DNA-templated

Inferred from direct assay PubMed 12949260. Source: UniProtKB

negative regulation of wound healing

Inferred from genetic interaction PubMed 23027131. Source: BHF-UCL

neuron differentiation

Inferred from Biological aspect of Ancestor. Source: RefGenome

non-canonical Wnt signaling pathway

Inferred from direct assay PubMed 18617424. Source: MGI

non-canonical Wnt signaling pathway via MAPK cascade

Inferred from electronic annotation. Source: Ensembl

oocyte development

Inferred from mutant phenotype PubMed 9989404. Source: MGI

paramesonephric duct development

Inferred from mutant phenotype PubMed 14695376. Source: UniProtKB

positive regulation of Rho GTPase activity

Inferred from genetic interaction PubMed 23027131. Source: BHF-UCL

positive regulation of aldosterone biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of bone mineralization

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of canonical Wnt signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of collagen biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cortisol biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of dermatome development

Inferred from sequence or structural similarity PubMed 10654605. Source: BHF-UCL

positive regulation of focal adhesion assembly

Inferred from genetic interaction PubMed 23027131. Source: BHF-UCL

positive regulation of meiosis

Inferred from genetic interaction PubMed 20106871. Source: MGI

positive regulation of osteoblast differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of stress fiber assembly

Inferred from genetic interaction PubMed 23027131. Source: BHF-UCL

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 11283799. Source: UniProtKB

protein palmitoylation

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of cell-cell adhesion

Inferred from mutant phenotype PubMed 20106871. Source: MGI

renal vesicle formation

Inferred from mutant phenotype PubMed 16054034. Source: MGI

renal vesicle induction

Inferred from direct assay PubMed 11507767. Source: MGI

sex differentiation

Inferred from mutant phenotype PubMed 9989404. Source: MGI

signal transduction

Traceable author statement PubMed 9889131. Source: MGI

smooth muscle cell differentiation

Inferred from direct assay PubMed 16546160. Source: MGI

somatotropin secreting cell differentiation

Inferred from mutant phenotype PubMed 18351662. Source: MGI

tertiary branching involved in mammary gland duct morphogenesis

Inferred from mutant phenotype PubMed 10733525. Source: MGI

thyroid-stimulating hormone-secreting cell differentiation

Inferred from mutant phenotype PubMed 18351662. Source: MGI

tube morphogenesis

Inferred from mutant phenotype PubMed 16546160. Source: MGI

   Cellular_componentcell surface

Inferred from direct assay PubMed 8167409. Source: BHF-UCL

cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

endoplasmic reticulum lumen

Traceable author statement. Source: Reactome

extracellular matrix

Inferred from direct assay PubMed 8167409. Source: MGI

extracellular space

Inferred from sequence or structural similarity. Source: UniProtKB

proteinaceous extracellular matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionfrizzled binding

Inferred from physical interaction PubMed 15265686. Source: BHF-UCL

receptor binding

Traceable author statement PubMed 9889131. Source: MGI

transcription corepressor activity

Inferred from direct assay PubMed 12949260. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

KlO350822EBI-1570945,EBI-1570828

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 351329Protein Wnt-4
PRO_0000041422

Amino acid modifications

Lipidation781S-palmitoyl cysteine By similarity
Lipidation2121O-palmitoyl serine; by PORCN By similarity
Glycosylation881N-linked (GlcNAc...) Potential
Glycosylation2971N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P22724 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 7E1C5C739BE939D9

FASTA35139,050
        10         20         30         40         50         60 
MSPRSCLRSL RLLVFAVFSA AASNWLYLAK LSSVGSISEE ETCEKLKGLI QRQVQMCKRN 

        70         80         90        100        110        120 
LEVMDSVRRG AQLAIEECQY QFRNRRWNCS TLDSLPVFGK VVTQGTREAA FVYAISSAGV 

       130        140        150        160        170        180 
AFAVTRACSS GELEKCGCDR TVHGVSPQGF QWSGCSDNIA YGVAFSQSFV DVRERSKGAS 

       190        200        210        220        230        240 
SSRALMNLHN NEAGRKAILT HMRVECKCHG VSGSCEVKTC WRAVPPFRQV GHALKEKFDG 

       250        260        270        280        290        300 
ATEVEPRRVG SSRALVPRNA QFKPHTDEDL VYLEPSPDFC EQDIRSGVLG TRGRTCNKTS 

       310        320        330        340        350 
KAIDGCELLC CGRGFHTAQV ELAERCGCRF HWCCFVKCRQ CQRLVEMHTC R 

« Hide

References

[1]"Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development."
Gavin B.J., McMahon J.A., McMahon A.P.
Genes Dev. 4:2319-2332(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family."
Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T.
Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PORCN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M89797 mRNA. Translation: AAA40566.1.
PIRC36470.
RefSeqNP_033549.1. NM_009523.2.
UniGeneMm.20355.

3D structure databases

ProteinModelPortalP22724.
SMRP22724. Positions 66-350.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204576. 4 interactions.
IntActP22724. 2 interactions.
STRING10090.ENSMUSP00000036580.

PTM databases

PhosphoSiteP22724.

Proteomic databases

PRIDEP22724.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000045747; ENSMUSP00000036580; ENSMUSG00000036856.
GeneID22417.
KEGGmmu:22417.
UCSCuc008viv.2. mouse.

Organism-specific databases

CTD54361.
MGIMGI:98957. Wnt4.

Phylogenomic databases

eggNOGNOG284879.
HOGENOMHOG000039529.
HOVERGENHBG001595.
InParanoidP22724.
KOK00408.
OMAQVQICKR.
OrthoDBEOG7C8GJ8.
PhylomeDBP22724.
TreeFamTF105310.

Enzyme and pathway databases

ReactomeREACT_188257. Signal Transduction.

Gene expression databases

BgeeP22724.
CleanExMM_WNT4.
GenevestigatorP22724.

Family and domain databases

InterProIPR005817. Wnt.
IPR009142. Wnt4.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF19. PTHR12027:SF19. 1 hit.
PfamPF00110. wnt. 1 hit.
[Graphical view]
PRINTSPR01844. WNT4PROTEIN.
PR01349. WNTPROTEIN.
SMARTSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302837.
PROP22724.
SOURCESearch...

Entry information

Entry nameWNT4_MOUSE
AccessionPrimary (citable) accession number: P22724
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: April 16, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot