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Protein

Protein Wnt-4

Gene

Wnt4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. May be an intracellular signaling molecule involved in segmentation of the forebrain. Is likely to signal over only few cell diameters (By similarity). Seems to be involved in kidney development.By similarity

GO - Molecular functioni

  1. frizzled binding Source: BHF-UCL
  2. receptor binding Source: MGI
  3. transcription corepressor activity Source: UniProtKB

GO - Biological processi

  1. adrenal gland development Source: Ensembl
  2. anatomical structure development Source: MGI
  3. androgen biosynthetic process Source: UniProtKB
  4. branching involved in ureteric bud morphogenesis Source: MGI
  5. branching morphogenesis of an epithelial tube Source: MGI
  6. canonical Wnt signaling pathway Source: UniProtKB
  7. cell-cell signaling Source: MGI
  8. cell differentiation Source: MGI
  9. cell fate commitment Source: MGI
  10. cellular response to starvation Source: MGI
  11. cellular response to transforming growth factor beta stimulus Source: Ensembl
  12. embryonic epithelial tube formation Source: MGI
  13. epithelial to mesenchymal transition Source: Ensembl
  14. establishment of protein localization to plasma membrane Source: UniProtKB
  15. female gonad development Source: UniProtKB
  16. female sex determination Source: UniProtKB
  17. gamete generation Source: MGI
  18. hormone metabolic process Source: MGI
  19. immature T cell proliferation in thymus Source: MGI
  20. kidney morphogenesis Source: MGI
  21. liver development Source: Ensembl
  22. male gonad development Source: UniProtKB
  23. mesenchymal to epithelial transition Source: MGI
  24. mesonephric tubule development Source: MGI
  25. mesonephros development Source: UniProtKB
  26. metanephric mesenchymal cell differentiation Source: UniProtKB
  27. metanephric nephron development Source: MGI
  28. metanephric nephron morphogenesis Source: MGI
  29. metanephric tubule formation Source: MGI
  30. metanephros development Source: MGI
  31. negative regulation of apoptotic signaling pathway Source: MGI
  32. negative regulation of canonical Wnt signaling pathway Source: UniProtKB
  33. negative regulation of cell differentiation Source: MGI
  34. negative regulation of cell migration Source: BHF-UCL
  35. negative regulation of fibroblast growth factor receptor signaling pathway Source: MGI
  36. negative regulation of gene expression Source: UniProtKB
  37. negative regulation of male gonad development Source: UniProtKB
  38. negative regulation of steroid biosynthetic process Source: UniProtKB
  39. negative regulation of testicular blood vessel morphogenesis Source: MGI
  40. negative regulation of testosterone biosynthetic process Source: UniProtKB
  41. negative regulation of transcription, DNA-templated Source: UniProtKB
  42. negative regulation of wound healing Source: BHF-UCL
  43. nephron development Source: MGI
  44. neuron differentiation Source: GO_Central
  45. non-canonical Wnt signaling pathway Source: MGI
  46. non-canonical Wnt signaling pathway via MAPK cascade Source: MGI
  47. oocyte development Source: MGI
  48. paramesonephric duct development Source: UniProtKB
  49. positive regulation of aldosterone biosynthetic process Source: UniProtKB
  50. positive regulation of bone mineralization Source: UniProtKB
  51. positive regulation of canonical Wnt signaling pathway Source: UniProtKB
  52. positive regulation of collagen biosynthetic process Source: UniProtKB
  53. positive regulation of cortisol biosynthetic process Source: UniProtKB
  54. positive regulation of dermatome development Source: BHF-UCL
  55. positive regulation of focal adhesion assembly Source: BHF-UCL
  56. positive regulation of meiosis Source: MGI
  57. positive regulation of osteoblast differentiation Source: UniProtKB
  58. positive regulation of Rho GTPase activity Source: BHF-UCL
  59. positive regulation of stress fiber assembly Source: BHF-UCL
  60. positive regulation of transcription, DNA-templated Source: UniProtKB
  61. protein palmitoylation Source: UniProtKB
  62. regulation of cell-cell adhesion Source: MGI
  63. renal vesicle formation Source: MGI
  64. renal vesicle induction Source: MGI
  65. sex differentiation Source: MGI
  66. signal transduction Source: MGI
  67. smooth muscle cell differentiation Source: MGI
  68. somatotropin secreting cell differentiation Source: MGI
  69. T cell differentiation in thymus Source: MGI
  70. tertiary branching involved in mammary gland duct morphogenesis Source: MGI
  71. thyroid-stimulating hormone-secreting cell differentiation Source: MGI
  72. tube morphogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

ReactomeiREACT_210612. negative regulation of TCF-dependent signaling by WNT ligand antagonists.
REACT_213918. WNT ligand biogenesis and trafficking.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-4
Gene namesi
Name:Wnt4
Synonyms:Wnt-4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:98957. Wnt4.

Subcellular locationi

GO - Cellular componenti

  1. cell surface Source: BHF-UCL
  2. cytoplasm Source: UniProtKB
  3. endoplasmic reticulum lumen Source: Reactome
  4. extracellular region Source: Reactome
  5. extracellular space Source: UniProtKB
  6. proteinaceous extracellular matrix Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 351329Protein Wnt-4PRO_0000041422Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi78 ↔ 89By similarity
Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi128 ↔ 136By similarity
Disulfide bondi138 ↔ 155By similarity
Disulfide bondi206 ↔ 220By similarity
Disulfide bondi208 ↔ 215By similarity
Lipidationi212 – 2121O-palmitoyl serine; by PORCNBy similarity
Disulfide bondi296 ↔ 311By similarity
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi326 ↔ 341By similarity
Disulfide bondi328 ↔ 338By similarity
Disulfide bondi333 ↔ 334By similarity

Post-translational modificationi

Palmitoylation at Ser-212 is required for efficient binding to frizzled receptors. Palmitoylation is necessary for proper trafficking to cell surface (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PRIDEiP22724.

PTM databases

PhosphoSiteiP22724.

Expressioni

Tissue specificityi

In adults in lung and brain.

Gene expression databases

BgeeiP22724.
CleanExiMM_WNT4.
ExpressionAtlasiP22724. baseline and differential.
GenevestigatoriP22724.

Interactioni

Subunit structurei

Interacts with PORCN.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
KlO350822EBI-1570945,EBI-1570828

Protein-protein interaction databases

BioGridi204576. 4 interactions.
IntActiP22724. 2 interactions.
STRINGi10090.ENSMUSP00000036580.

Structurei

3D structure databases

ProteinModelPortaliP22724.
SMRiP22724. Positions 66-350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG284879.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiP22724.
KOiK00408.
OMAiRQVQICK.
OrthoDBiEOG7C8GJ8.
PhylomeDBiP22724.
TreeFamiTF105310.

Family and domain databases

InterProiIPR005817. Wnt.
IPR009142. Wnt4.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01844. WNT4PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPRSCLRSL RLLVFAVFSA AASNWLYLAK LSSVGSISEE ETCEKLKGLI
60 70 80 90 100
QRQVQMCKRN LEVMDSVRRG AQLAIEECQY QFRNRRWNCS TLDSLPVFGK
110 120 130 140 150
VVTQGTREAA FVYAISSAGV AFAVTRACSS GELEKCGCDR TVHGVSPQGF
160 170 180 190 200
QWSGCSDNIA YGVAFSQSFV DVRERSKGAS SSRALMNLHN NEAGRKAILT
210 220 230 240 250
HMRVECKCHG VSGSCEVKTC WRAVPPFRQV GHALKEKFDG ATEVEPRRVG
260 270 280 290 300
SSRALVPRNA QFKPHTDEDL VYLEPSPDFC EQDIRSGVLG TRGRTCNKTS
310 320 330 340 350
KAIDGCELLC CGRGFHTAQV ELAERCGCRF HWCCFVKCRQ CQRLVEMHTC

R
Length:351
Mass (Da):39,050
Last modified:August 1, 1991 - v1
Checksum:i7E1C5C739BE939D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89797 mRNA. Translation: AAA40566.1.
CCDSiCCDS18815.1.
PIRiC36470.
RefSeqiNP_033549.1. NM_009523.2.
UniGeneiMm.20355.

Genome annotation databases

EnsembliENSMUST00000045747; ENSMUSP00000036580; ENSMUSG00000036856.
GeneIDi22417.
KEGGimmu:22417.
UCSCiuc008viv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M89797 mRNA. Translation: AAA40566.1.
CCDSiCCDS18815.1.
PIRiC36470.
RefSeqiNP_033549.1. NM_009523.2.
UniGeneiMm.20355.

3D structure databases

ProteinModelPortaliP22724.
SMRiP22724. Positions 66-350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204576. 4 interactions.
IntActiP22724. 2 interactions.
STRINGi10090.ENSMUSP00000036580.

PTM databases

PhosphoSiteiP22724.

Proteomic databases

PRIDEiP22724.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045747; ENSMUSP00000036580; ENSMUSG00000036856.
GeneIDi22417.
KEGGimmu:22417.
UCSCiuc008viv.2. mouse.

Organism-specific databases

CTDi54361.
MGIiMGI:98957. Wnt4.

Phylogenomic databases

eggNOGiNOG284879.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiP22724.
KOiK00408.
OMAiRQVQICK.
OrthoDBiEOG7C8GJ8.
PhylomeDBiP22724.
TreeFamiTF105310.

Enzyme and pathway databases

ReactomeiREACT_210612. negative regulation of TCF-dependent signaling by WNT ligand antagonists.
REACT_213918. WNT ligand biogenesis and trafficking.

Miscellaneous databases

NextBioi302837.
PROiP22724.
SOURCEiSearch...

Gene expression databases

BgeeiP22724.
CleanExiMM_WNT4.
ExpressionAtlasiP22724. baseline and differential.
GenevestigatoriP22724.

Family and domain databases

InterProiIPR005817. Wnt.
IPR009142. Wnt4.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01844. WNT4PROTEIN.
PR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development."
    Gavin B.J., McMahon J.A., McMahon A.P.
    Genes Dev. 4:2319-2332(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family."
    Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T.
    Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PORCN.

Entry informationi

Entry nameiWNT4_MOUSE
AccessioniPrimary (citable) accession number: P22724
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: March 4, 2015
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.