Reviewed, UniProtKB/Swiss-Prot P22724 (WNT4_MOUSE)

Names and origin

Protein names

Recommended name:
    Protein Wnt-4

Gene names

Name:	Wnt4
Synonyms:	Wnt-4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. May be an intracellular signaling molecule involved in segmentation of the forebrain. Is likely to signal over only few cell diameters By similarity. Seems to be involved in kidney development.
Subunit structure	Interacts with PORCN. Ref.2
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	In adults in lung and brain.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway, calcium modulating pathway Inferred from electronic annotation. Source: InterPro cell-cell signaling Traceable author statement. Source: MGI embryonic epithelial tube formation Inferred from direct assay. Source: MGI female gonad development Inferred from mutant phenotype. Source: MGI hormone metabolic process Inferred from mutant phenotype. Source: MGI male gonad development Inferred from mutant phenotype. Source: MGI negative regulation of cell differentiation Inferred from mutant phenotype. Source: MGI negative regulation of fibroblast growth factor receptor signaling pathway Inferred from genetic interaction. Source: MGI oocyte development Inferred from mutant phenotype. Source: MGI smooth muscle cell differentiation Inferred from direct assay. Source: MGI
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	receptor binding Traceable author statement. Source: MGI signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Kl	O35082	1	EBI-1570945,EBI-1570828

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 351	329	Protein Wnt-4		PRO_0000041422
Amino acid modifications
	Glycosylation	88	1	N-linked (GlcNAc...) Potential
	Glycosylation	297	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P22724-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 7E1C5C739BE939D9	FASTA	351	39,050
	10 20 30 40 50 60 MSPRSCLRSL RLLVFAVFSA AASNWLYLAK LSSVGSISEE ETCEKLKGLI QRQVQMCKRN 70 80 90 100 110 120 LEVMDSVRRG AQLAIEECQY QFRNRRWNCS TLDSLPVFGK VVTQGTREAA FVYAISSAGV 130 140 150 160 170 180 AFAVTRACSS GELEKCGCDR TVHGVSPQGF QWSGCSDNIA YGVAFSQSFV DVRERSKGAS 190 200 210 220 230 240 SSRALMNLHN NEAGRKAILT HMRVECKCHG VSGSCEVKTC WRAVPPFRQV GHALKEKFDG 250 260 270 280 290 300 ATEVEPRRVG SSRALVPRNA QFKPHTDEDL VYLEPSPDFC EQDIRSGVLG TRGRTCNKTS 310 320 330 340 350 KAIDGCELLC CGRGFHTAQV ELAERCGCRF HWCCFVKCRQ CQRLVEMHTC R

References

[1]	"Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development." Gavin B.J., McMahon J.A., McMahon A.P. Genes Dev. 4:2319-2332(1990) [PubMed: 2279700] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family." Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T. Eur. J. Biochem. 267:4300-4311(2000) [PubMed: 10866835] [Abstract] Cited for: INTERACTION WITH PORCN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M89797 mRNA. Translation: AAA40566.1.
IPI	IPI00131373.
PIR	C36470.
RefSeq	NP_033549.1.
UniGene	Mm.20355
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P22724. 1 interaction.
Genome annotation databases
Ensembl	ENSMUSG00000036856. Mus musculus. [Contig view]
GeneID	22417.
KEGG	mmu:22417.
Organism-specific databases
MGI	MGI:98957. Wnt4.
Phylogenomic databases
HOGENOM	P22724.
HOVERGEN	P22724.
OMA	P22724. SHMRVEC.
Gene expression databases
ArrayExpress	P22724.
Bgee	P22724.
CleanEx	MM_WNT4.
GermOnline	ENSMUSG00000036856. Mus musculus.
Family and domain databases
InterPro	IPR009142. Wnt4. IPR005816. Wnt_grthfactor. IPR018161. Wnt_grthfactor_CS. IPR005817. Wnt_SF. [Graphical view]
PANTHER	PTHR12027. Wnt. 1 hit. PTHR12027:SF19. Wnt4. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01844. WNT4PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	302837.
SOURCE	Search...

Entry information

Entry name

WNT4_MOUSE

Accession

Primary (citable) accession number: P22724

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)