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P22724

- WNT4_MOUSE

UniProt

P22724 - WNT4_MOUSE

Protein

Protein Wnt-4

Gene

Wnt4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    Ligand for members of the frizzled family of seven transmembrane receptors. May be an intracellular signaling molecule involved in segmentation of the forebrain. Is likely to signal over only few cell diameters By similarity. Seems to be involved in kidney development.By similarity

    GO - Molecular functioni

    1. frizzled binding Source: BHF-UCL
    2. protein binding Source: IntAct
    3. receptor binding Source: MGI
    4. transcription corepressor activity Source: UniProtKB

    GO - Biological processi

    1. adrenal gland development Source: Ensembl
    2. anatomical structure development Source: MGI
    3. androgen biosynthetic process Source: UniProtKB
    4. branching involved in ureteric bud morphogenesis Source: MGI
    5. branching morphogenesis of an epithelial tube Source: MGI
    6. canonical Wnt signaling pathway Source: UniProtKB
    7. cell-cell signaling Source: MGI
    8. cell differentiation Source: MGI
    9. cell fate commitment Source: MGI
    10. cellular response to starvation Source: MGI
    11. cellular response to transforming growth factor beta stimulus Source: Ensembl
    12. embryonic epithelial tube formation Source: MGI
    13. epithelial to mesenchymal transition Source: Ensembl
    14. establishment of protein localization to plasma membrane Source: UniProtKB
    15. female gonad development Source: UniProtKB
    16. female sex determination Source: UniProtKB
    17. gamete generation Source: MGI
    18. hormone metabolic process Source: MGI
    19. immature T cell proliferation in thymus Source: MGI
    20. kidney morphogenesis Source: MGI
    21. liver development Source: Ensembl
    22. male gonad development Source: UniProtKB
    23. mesenchymal to epithelial transition Source: MGI
    24. mesonephric tubule development Source: MGI
    25. mesonephros development Source: UniProtKB
    26. metanephric mesenchymal cell differentiation Source: UniProtKB
    27. metanephric nephron development Source: MGI
    28. metanephric nephron morphogenesis Source: MGI
    29. metanephric tubule formation Source: MGI
    30. metanephros development Source: MGI
    31. negative regulation of apoptotic signaling pathway Source: MGI
    32. negative regulation of canonical Wnt signaling pathway Source: UniProtKB
    33. negative regulation of cell differentiation Source: MGI
    34. negative regulation of cell migration Source: BHF-UCL
    35. negative regulation of fibroblast growth factor receptor signaling pathway Source: MGI
    36. negative regulation of gene expression Source: UniProtKB
    37. negative regulation of male gonad development Source: UniProtKB
    38. negative regulation of steroid biosynthetic process Source: UniProtKB
    39. negative regulation of testicular blood vessel morphogenesis Source: Ensembl
    40. negative regulation of testosterone biosynthetic process Source: UniProtKB
    41. negative regulation of transcription, DNA-templated Source: UniProtKB
    42. negative regulation of wound healing Source: BHF-UCL
    43. neuron differentiation Source: RefGenome
    44. non-canonical Wnt signaling pathway Source: MGI
    45. non-canonical Wnt signaling pathway via MAPK cascade Source: Ensembl
    46. oocyte development Source: MGI
    47. paramesonephric duct development Source: UniProtKB
    48. positive regulation of aldosterone biosynthetic process Source: UniProtKB
    49. positive regulation of bone mineralization Source: UniProtKB
    50. positive regulation of canonical Wnt signaling pathway Source: UniProtKB
    51. positive regulation of collagen biosynthetic process Source: UniProtKB
    52. positive regulation of cortisol biosynthetic process Source: UniProtKB
    53. positive regulation of dermatome development Source: BHF-UCL
    54. positive regulation of focal adhesion assembly Source: BHF-UCL
    55. positive regulation of meiosis Source: MGI
    56. positive regulation of osteoblast differentiation Source: UniProtKB
    57. positive regulation of Rho GTPase activity Source: BHF-UCL
    58. positive regulation of stress fiber assembly Source: BHF-UCL
    59. positive regulation of transcription, DNA-templated Source: UniProtKB
    60. protein palmitoylation Source: UniProtKB
    61. regulation of cell-cell adhesion Source: MGI
    62. renal vesicle formation Source: MGI
    63. renal vesicle induction Source: MGI
    64. sex differentiation Source: MGI
    65. signal transduction Source: MGI
    66. smooth muscle cell differentiation Source: MGI
    67. somatotropin secreting cell differentiation Source: MGI
    68. T cell differentiation in thymus Source: MGI
    69. tertiary branching involved in mammary gland duct morphogenesis Source: MGI
    70. thyroid-stimulating hormone-secreting cell differentiation Source: MGI
    71. tube morphogenesis Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Wnt signaling pathway

    Enzyme and pathway databases

    ReactomeiREACT_210612. negative regulation of TCF-dependent signaling by WNT ligand antagonists.
    REACT_213918. WNT ligand biogenesis and trafficking.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein Wnt-4
    Gene namesi
    Name:Wnt4
    Synonyms:Wnt-4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:98957. Wnt4.

    Subcellular locationi

    GO - Cellular componenti

    1. cell surface Source: BHF-UCL
    2. cytoplasm Source: UniProtKB
    3. endoplasmic reticulum lumen Source: Reactome
    4. extracellular matrix Source: MGI
    5. extracellular space Source: UniProtKB
    6. proteinaceous extracellular matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Extracellular matrix, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 351329Protein Wnt-4PRO_0000041422Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi78 ↔ 89By similarity
    Glycosylationi88 – 881N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi128 ↔ 136By similarity
    Disulfide bondi138 ↔ 155By similarity
    Disulfide bondi206 ↔ 220By similarity
    Disulfide bondi208 ↔ 215By similarity
    Lipidationi212 – 2121O-palmitoyl serine; by PORCNBy similarity
    Disulfide bondi296 ↔ 311By similarity
    Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi326 ↔ 341By similarity
    Disulfide bondi328 ↔ 338By similarity
    Disulfide bondi333 ↔ 334By similarity

    Post-translational modificationi

    Palmitoylation at Ser-212 is required for efficient binding to frizzled receptors. Palmitoylation is necessary for proper trafficking to cell surface By similarity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PRIDEiP22724.

    PTM databases

    PhosphoSiteiP22724.

    Expressioni

    Tissue specificityi

    In adults in lung and brain.

    Gene expression databases

    BgeeiP22724.
    CleanExiMM_WNT4.
    GenevestigatoriP22724.

    Interactioni

    Subunit structurei

    Interacts with PORCN.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    KlO350822EBI-1570945,EBI-1570828

    Protein-protein interaction databases

    BioGridi204576. 4 interactions.
    IntActiP22724. 2 interactions.
    STRINGi10090.ENSMUSP00000036580.

    Structurei

    3D structure databases

    ProteinModelPortaliP22724.
    SMRiP22724. Positions 66-350.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Wnt family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG284879.
    HOGENOMiHOG000039529.
    HOVERGENiHBG001595.
    InParanoidiP22724.
    KOiK00408.
    OMAiQVQICKR.
    OrthoDBiEOG7C8GJ8.
    PhylomeDBiP22724.
    TreeFamiTF105310.

    Family and domain databases

    InterProiIPR005817. Wnt.
    IPR009142. Wnt4.
    IPR018161. Wnt_CS.
    [Graphical view]
    PANTHERiPTHR12027. PTHR12027. 1 hit.
    PfamiPF00110. wnt. 1 hit.
    [Graphical view]
    PRINTSiPR01844. WNT4PROTEIN.
    PR01349. WNTPROTEIN.
    SMARTiSM00097. WNT1. 1 hit.
    [Graphical view]
    PROSITEiPS00246. WNT1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22724-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSPRSCLRSL RLLVFAVFSA AASNWLYLAK LSSVGSISEE ETCEKLKGLI    50
    QRQVQMCKRN LEVMDSVRRG AQLAIEECQY QFRNRRWNCS TLDSLPVFGK 100
    VVTQGTREAA FVYAISSAGV AFAVTRACSS GELEKCGCDR TVHGVSPQGF 150
    QWSGCSDNIA YGVAFSQSFV DVRERSKGAS SSRALMNLHN NEAGRKAILT 200
    HMRVECKCHG VSGSCEVKTC WRAVPPFRQV GHALKEKFDG ATEVEPRRVG 250
    SSRALVPRNA QFKPHTDEDL VYLEPSPDFC EQDIRSGVLG TRGRTCNKTS 300
    KAIDGCELLC CGRGFHTAQV ELAERCGCRF HWCCFVKCRQ CQRLVEMHTC 350
    R 351
    Length:351
    Mass (Da):39,050
    Last modified:August 1, 1991 - v1
    Checksum:i7E1C5C739BE939D9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M89797 mRNA. Translation: AAA40566.1.
    CCDSiCCDS18815.1.
    PIRiC36470.
    RefSeqiNP_033549.1. NM_009523.2.
    UniGeneiMm.20355.

    Genome annotation databases

    EnsembliENSMUST00000045747; ENSMUSP00000036580; ENSMUSG00000036856.
    GeneIDi22417.
    KEGGimmu:22417.
    UCSCiuc008viv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M89797 mRNA. Translation: AAA40566.1 .
    CCDSi CCDS18815.1.
    PIRi C36470.
    RefSeqi NP_033549.1. NM_009523.2.
    UniGenei Mm.20355.

    3D structure databases

    ProteinModelPortali P22724.
    SMRi P22724. Positions 66-350.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204576. 4 interactions.
    IntActi P22724. 2 interactions.
    STRINGi 10090.ENSMUSP00000036580.

    PTM databases

    PhosphoSitei P22724.

    Proteomic databases

    PRIDEi P22724.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000045747 ; ENSMUSP00000036580 ; ENSMUSG00000036856 .
    GeneIDi 22417.
    KEGGi mmu:22417.
    UCSCi uc008viv.2. mouse.

    Organism-specific databases

    CTDi 54361.
    MGIi MGI:98957. Wnt4.

    Phylogenomic databases

    eggNOGi NOG284879.
    HOGENOMi HOG000039529.
    HOVERGENi HBG001595.
    InParanoidi P22724.
    KOi K00408.
    OMAi QVQICKR.
    OrthoDBi EOG7C8GJ8.
    PhylomeDBi P22724.
    TreeFami TF105310.

    Enzyme and pathway databases

    Reactomei REACT_210612. negative regulation of TCF-dependent signaling by WNT ligand antagonists.
    REACT_213918. WNT ligand biogenesis and trafficking.

    Miscellaneous databases

    NextBioi 302837.
    PROi P22724.
    SOURCEi Search...

    Gene expression databases

    Bgeei P22724.
    CleanExi MM_WNT4.
    Genevestigatori P22724.

    Family and domain databases

    InterProi IPR005817. Wnt.
    IPR009142. Wnt4.
    IPR018161. Wnt_CS.
    [Graphical view ]
    PANTHERi PTHR12027. PTHR12027. 1 hit.
    Pfami PF00110. wnt. 1 hit.
    [Graphical view ]
    PRINTSi PR01844. WNT4PROTEIN.
    PR01349. WNTPROTEIN.
    SMARTi SM00097. WNT1. 1 hit.
    [Graphical view ]
    PROSITEi PS00246. WNT1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development."
      Gavin B.J., McMahon J.A., McMahon A.P.
      Genes Dev. 4:2319-2332(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The evolutionarily conserved porcupine gene family is involved in the processing of the Wnt family."
      Tanaka K., Okabayashi H., Asashima M., Perrimon N., Kadowaki T.
      Eur. J. Biochem. 267:4300-4311(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PORCN.

    Entry informationi

    Entry nameiWNT4_MOUSE
    AccessioniPrimary (citable) accession number: P22724
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3