Skip Header

Contribute Send feedback
Read comments (?) or add your own

P22723 (GBRG2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Gamma-aminobutyric acid receptor subunit gamma-2
Alternative name(s):
GABA(A) receptor subunit gamma-2
Gene names
Name:Gabrg2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

Subunit structure

Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.

Subcellular location

Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Post-translational modification

Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability. Ref.5 Ref.6

Miscellaneous

This subunit carries the benzodiazepine binding site.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG2 sub-subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   LigandChloride
   Molecular functionChloride channel
Ion channel
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadult behavior

Inferred from mutant phenotype PubMed 15850489. Source: MGI

gamma-aminobutyric acid signaling pathway

Inferred from direct assay PubMed 12574411. Source: MGI

post-embryonic development

Inferred from mutant phenotype PubMed 15850489. Source: MGI

synaptic transmission

Inferred from direct assay PubMed 12574411. Source: MGI

synaptic transmission, GABAergic

Inferred from electronic annotation. Source: Compara

   Cellular_componentaxon

Inferred from direct assay PubMed 9682826. Source: MGI

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

chloride channel complex

Inferred from electronic annotation. Source: UniProtKB-KW

cytoplasm

Inferred from electronic annotation. Source: Compara

dendrite membrane

Inferred from electronic annotation. Source: Compara

inhibitory synapse

Inferred from electronic annotation. Source: Compara

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGABA-A receptor activity

Inferred from direct assay PubMed 12574411. Source: MGI

chloride channel activity

Inferred from electronic annotation. Source: UniProtKB-KW

extracellular ligand-gated ion channel activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2L (identifier: P22723-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2S (identifier: P22723-2)

The sequence of this isoform differs from the canonical sequence as follows:
     376-383: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3838 Potential
Chain39 – 474436Gamma-aminobutyric acid receptor subunit gamma-2
PRO_0000000478

Regions

Topological domain39 – 272234Extracellular Probable
Transmembrane273 – 29523Helical; Probable
Transmembrane299 – 32123Helical; Probable
Transmembrane333 – 35523Helical; Probable
Topological domain356 – 45095Cytoplasmic Probable
Transmembrane451 – 47424Helical; Probable

Amino acid modifications

Modified residue3811Phosphoserine; by PKC Probable
Glycosylation511N-linked (GlcNAc...) Potential
Glycosylation1281N-linked (GlcNAc...) Potential
Glycosylation2461N-linked (GlcNAc...) Potential
Disulfide bond189 ↔ 203 By similarity

Natural variations

Alternative sequence376 – 3838Missing in isoform 2S.
VSP_000091
Natural variant491A → T in strain: DBA/2J.

Sequences

Sequence LengthMass (Da)Tools
Isoform 2L [UniParc].

Last modified March 27, 2002. Version 3.
Checksum: 45BD61649C296EA9

FASTA47455,099
        10         20         30         40         50         60 
MSSPNTWSIG SSVYSPVFSQ KMTLWILLLL SLYPGFTSQK SDDDYEDYAS NKTWVLTPKV 

        70         80         90        100        110        120 
PEGDVTVILN NLLEGYDNKL RPDIGVKPTL IHTDMYVNSI GPVNAINMEY TIDIFFAQTW 

       130        140        150        160        170        180 
YDRRLKFNST IKVLRLNSNM VGKIWIPDTF FRNSKKADAH WITTPNRMLR IWNDGRVLYT 

       190        200        210        220        230        240 
LRLTIDAECQ LQLHNFPMDE HSCPLEFSSY GYPREEIVYQ WKRSSVEVGD TRSWRLYQFS 

       250        260        270        280        290        300 
FVGLRNTTEV VKTTSGDYVV MSVYFDLSRR MGYFTIQTYI PCTLIVVLSW VSFWINKDAV 

       310        320        330        340        350        360 
PARTSLGITT VLTMTTLSTI ARKSLPKVSY VTAMDLFVSV CFIFVFSALV EYGTLHYFVS 

       370        380        390        400        410        420 
NRKPSKDKDK KKKNPLLRMF SFKAPTIDIR PRSATIQMNN ATHLQERDEE YGYECLDGKD 

       430        440        450        460        470 
CASFFCCFED CRTGAWRHGR IHIRIAKMDS YARIFFPTAF CLFNLVYWVS YLYL

« Hide

Isoform 2S [UniParc].

Checksum: 670A0E228975A4AE
Show »

FASTA46654,075

References

« Hide 'large scale' references
[1]"Generation of two forms of the gamma-aminobutyric acidA receptor gamma 2-subunit in mice by alternative splicing."
Kofuji P., Wang J.B., Moss S.J., Huganir R.L., Burt D.R.
J. Neurochem. 56:713-715(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2L AND 2S).
[2]Sikela J.M., Shaw W.D., Khan A.S., Lin L.-H., Leidenheimer G., Siegel R.E.
Submitted (APR-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2L).
[3]"Allelic variation in the GABA A receptor gamma2 subunit is associated with genetic susceptibility to ethanol-induced motor incoordination and hypothermia, conditioned taste aversion, and withdrawal in BXD/Ty recombinant inbred mice."
Hood H.M., Buck K.J.
Alcohol. Clin. Exp. Res. 24:1327-1334(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2L).
Strain: C57BL/6J, DBA/2J and Various BXD strains.
Tissue: Brain.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2L).
Tissue: Retina.
[5]"The gamma2 subunit of GABA(A) receptors is a substrate for palmitoylation by GODZ."
Keller C.A., Yuan X., Panzanelli P., Martin M.L., Alldred M., Sassoe-Pognetto M., Luescher B.
J. Neurosci. 24:5881-5891(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PALMITOYLATION.
[6]"Palmitoylation regulates the clustering and cell surface stability of GABAA receptors."
Rathenberg J., Kittler J.T., Moss S.J.
Mol. Cell. Neurosci. 26:251-257(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PALMITOYLATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M86572 mRNA. Translation: AAB59635.1.
M62374 mRNA. Translation: AAA37653.1.
AF233775 mRNA. Translation: AAK71573.1.
AF233776 mRNA. Translation: AAK71574.1.
AF233777 mRNA. Translation: AAK71575.1.
AF233778 mRNA. Translation: AAK71576.1.
AF233779 mRNA. Translation: AAK71577.1.
AF233780 mRNA. Translation: AAK71578.1.
AF233781 mRNA. Translation: AAK71579.1.
AF233782 mRNA. Translation: AAK71580.1.
AF233783 mRNA. Translation: AAK71581.1.
AF233784 mRNA. Translation: AAK71582.1.
AF233785 mRNA. Translation: AAK71583.1.
AF233786 mRNA. Translation: AAK71584.1.
AF233787 mRNA. Translation: AAK71585.1.
AF233788 mRNA. Translation: AAK71586.1.
AF233789 mRNA. Translation: AAK71587.1.
AF233790 mRNA. Translation: AAK71588.1.
AF233791 mRNA. Translation: AAK71589.1.
AF233792 mRNA. Translation: AAK71590.1.
AF233793 mRNA. Translation: AAK71591.1.
AF233794 mRNA. Translation: AAK71592.1.
AF233795 mRNA. Translation: AAK71593.1.
AF233796 mRNA. Translation: AAK71594.1.
AF233797 mRNA. Translation: AAK71595.1.
AF233798 mRNA. Translation: AAK71596.1.
AF233799 mRNA. Translation: AAK71597.1.
AF233800 mRNA. Translation: AAK71598.1.
AF233801 mRNA. Translation: AAK71599.1.
AF233802 mRNA. Translation: AAK71600.1.
BC031762 mRNA. Translation: AAH31762.1.
IPIIPI00131369.
IPI00228358.
PIRJH0316.
JH0317.
RefSeqNP_032099.1. NM_008073.3.
NP_803127.3. NM_177408.6.
UniGeneMm.5309.

3D structure databases

ProteinModelPortalP22723.
SMRP22723. Positions 64-377.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-48995N.

PTM databases

PhosphoSiteP22723.

Proteomic databases

PaxDbP22723.
PRIDEP22723.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000070725; ENSMUSP00000064739; ENSMUSG00000020436.
ENSMUST00000070735; ENSMUSP00000063812; ENSMUSG00000020436.
GeneID14406.
KEGGmmu:14406.
UCSCuc007imb.1. mouse.

Organism-specific databases

CTD2566.
MGIMGI:95623. Gabrg2.

Phylogenomic databases

eggNOGNOG238757.
GeneTreeENSGT00540000070314.
HOGENOMHOG000231337.
HOVERGENHBG051707.
InParanoidP22723.
KOK05186.
OMAGFTSQKS.
OrthoDBEOG44BB25.

Gene expression databases

ArrayExpressP22723.
BgeeP22723.
GenevestigatorP22723.
GermOnlineENSMUSG00000020436. Mus musculus.

Family and domain databases

Gene3D2.70.170.10. 1 hit.
InterProIPR006028. GABAA_rcpt.
IPR005439. GABBAg2_rcpt.
IPR005437. GABBAg_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR00253. GABAARECEPTR.
PR01620. GABAARGAMMA.
PR01622. GABAARGAMMA2.
PR00252. NRIONCHANNEL.
SUPFAMSSF90112. Neu_channel_TM. 1 hit.
SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP22723.
ChEMBLCHEMBL2941.
NextBio285971.
SOURCESearch...

Entry information

Entry nameGBRG2_MOUSE
AccessionPrimary (citable) accession number: P22723
Secondary accession number(s): Q91V50, Q91VA8
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: March 27, 2002
Last modified: May 1, 2013
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents