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Protein

Gamma-aminobutyric acid receptor subunit gamma-2

Gene

Gabrg2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.1 Publication

GO - Molecular functioni

  • chloride channel activity Source: UniProtKB
  • extracellular ligand-gated ion channel activity Source: InterPro
  • GABA-A receptor activity Source: UniProtKB

GO - Biological processi

  • adult behavior Source: MGI
  • cellular response to histamine Source: UniProtKB
  • chloride transmembrane transport Source: UniProtKB
  • gamma-aminobutyric acid signaling pathway Source: MGI
  • post-embryonic development Source: MGI
  • synaptic transmission Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiR-MMU-975298. Ligand-gated ion channel transport.
R-MMU-977441. GABA A receptor activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit gamma-2
Alternative name(s):
GABA(A) receptor subunit gamma-2
Gene namesi
Name:Gabrg2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:95623. Gabrg2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini39 – 272234ExtracellularCuratedAdd
BLAST
Transmembranei273 – 29523HelicalCuratedAdd
BLAST
Transmembranei299 – 32123HelicalCuratedAdd
BLAST
Transmembranei333 – 35523HelicalCuratedAdd
BLAST
Topological domaini356 – 45095CytoplasmicCuratedAdd
BLAST
Transmembranei451 – 47424HelicalCuratedAdd
BLAST

GO - Cellular componenti

  • axon Source: MGI
  • cell junction Source: UniProtKB-KW
  • chloride channel complex Source: UniProtKB-KW
  • cytoplasm Source: Ensembl
  • GABA-A receptor complex Source: UniProtKB
  • inhibitory synapse Source: MGI
  • integral component of plasma membrane Source: UniProtKB
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2094133.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3838Sequence analysisAdd
BLAST
Chaini39 – 474436Gamma-aminobutyric acid receptor subunit gamma-2PRO_0000000478Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi51 – 511N-linked (GlcNAc...)Sequence analysis
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence analysis
Disulfide bondi189 ↔ 203By similarity
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence analysis
Modified residuei381 – 3811Phosphoserine; by PKCCurated

Post-translational modificationi

Palmitoylated by ZDHHC3/GODZ; which may affect presynaptic clustering and/or cell surface stability.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP22723.
PaxDbiP22723.
PeptideAtlasiP22723.
PRIDEiP22723.

PTM databases

iPTMnetiP22723.
PhosphoSiteiP22723.
SwissPalmiP22723.

Expressioni

Gene expression databases

BgeeiP22723.
ExpressionAtlasiP22723. baseline and differential.
GenevisibleiP22723. MM.

Interactioni

Subunit structurei

Interacts with GABARAP (By similarity). Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains.By similarity1 Publication

Protein-protein interaction databases

BioGridi199808. 3 interactions.
DIPiDIP-48995N.
STRINGi10090.ENSMUSP00000063812.

Structurei

3D structure databases

ProteinModelPortaliP22723.
SMRiP22723. Positions 66-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3642. Eukaryota.
ENOG410XNQG. LUCA.
GeneTreeiENSGT00760000119010.
HOGENOMiHOG000231337.
HOVERGENiHBG051707.
InParanoidiP22723.
KOiK05186.
OMAiYPGFTSQ.
OrthoDBiEOG7JX342.
PhylomeDBiP22723.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR005439. GABBAg2_rcpt.
IPR005437. GABBAg_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01620. GABAARGAMMA.
PR01622. GABAARGAMMA2.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2L (identifier: P22723-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSPNTWSIG SSVYSPVFSQ KMTLWILLLL SLYPGFTSQK SDDDYEDYAS
60 70 80 90 100
NKTWVLTPKV PEGDVTVILN NLLEGYDNKL RPDIGVKPTL IHTDMYVNSI
110 120 130 140 150
GPVNAINMEY TIDIFFAQTW YDRRLKFNST IKVLRLNSNM VGKIWIPDTF
160 170 180 190 200
FRNSKKADAH WITTPNRMLR IWNDGRVLYT LRLTIDAECQ LQLHNFPMDE
210 220 230 240 250
HSCPLEFSSY GYPREEIVYQ WKRSSVEVGD TRSWRLYQFS FVGLRNTTEV
260 270 280 290 300
VKTTSGDYVV MSVYFDLSRR MGYFTIQTYI PCTLIVVLSW VSFWINKDAV
310 320 330 340 350
PARTSLGITT VLTMTTLSTI ARKSLPKVSY VTAMDLFVSV CFIFVFSALV
360 370 380 390 400
EYGTLHYFVS NRKPSKDKDK KKKNPLLRMF SFKAPTIDIR PRSATIQMNN
410 420 430 440 450
ATHLQERDEE YGYECLDGKD CASFFCCFED CRTGAWRHGR IHIRIAKMDS
460 470
YARIFFPTAF CLFNLVYWVS YLYL
Length:474
Mass (Da):55,099
Last modified:March 27, 2002 - v3
Checksum:i45BD61649C296EA9
GO
Isoform 2S (identifier: P22723-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     376-383: Missing.

Show »
Length:466
Mass (Da):54,075
Checksum:i670A0E228975A4AE
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti49 – 491A → T in strain: DBA/2J.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei376 – 3838Missing in isoform 2S. 1 PublicationVSP_000091

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86572 mRNA. Translation: AAB59635.1.
M62374 mRNA. Translation: AAA37653.1.
AF233775 mRNA. Translation: AAK71573.1.
AF233776 mRNA. Translation: AAK71574.1.
AF233777 mRNA. Translation: AAK71575.1.
AF233778 mRNA. Translation: AAK71576.1.
AF233779 mRNA. Translation: AAK71577.1.
AF233780 mRNA. Translation: AAK71578.1.
AF233781 mRNA. Translation: AAK71579.1.
AF233782 mRNA. Translation: AAK71580.1.
AF233783 mRNA. Translation: AAK71581.1.
AF233784 mRNA. Translation: AAK71582.1.
AF233785 mRNA. Translation: AAK71583.1.
AF233786 mRNA. Translation: AAK71584.1.
AF233787 mRNA. Translation: AAK71585.1.
AF233788 mRNA. Translation: AAK71586.1.
AF233789 mRNA. Translation: AAK71587.1.
AF233790 mRNA. Translation: AAK71588.1.
AF233791 mRNA. Translation: AAK71589.1.
AF233792 mRNA. Translation: AAK71590.1.
AF233793 mRNA. Translation: AAK71591.1.
AF233794 mRNA. Translation: AAK71592.1.
AF233795 mRNA. Translation: AAK71593.1.
AF233796 mRNA. Translation: AAK71594.1.
AF233797 mRNA. Translation: AAK71595.1.
AF233798 mRNA. Translation: AAK71596.1.
AF233799 mRNA. Translation: AAK71597.1.
AF233800 mRNA. Translation: AAK71598.1.
AF233801 mRNA. Translation: AAK71599.1.
AF233802 mRNA. Translation: AAK71600.1.
BC031762 mRNA. Translation: AAH31762.1.
CCDSiCCDS24551.1. [P22723-1]
CCDS24552.2. [P22723-2]
PIRiJH0316.
JH0317.
RefSeqiNP_032099.1. NM_008073.3. [P22723-1]
NP_803127.3. NM_177408.6. [P22723-2]
UniGeneiMm.5309.

Genome annotation databases

EnsembliENSMUST00000070725; ENSMUSP00000064739; ENSMUSG00000020436. [P22723-2]
ENSMUST00000070735; ENSMUSP00000063812; ENSMUSG00000020436. [P22723-1]
GeneIDi14406.
KEGGimmu:14406.
UCSCiuc007imb.2. mouse. [P22723-1]
uc007imc.3. mouse. [P22723-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86572 mRNA. Translation: AAB59635.1.
M62374 mRNA. Translation: AAA37653.1.
AF233775 mRNA. Translation: AAK71573.1.
AF233776 mRNA. Translation: AAK71574.1.
AF233777 mRNA. Translation: AAK71575.1.
AF233778 mRNA. Translation: AAK71576.1.
AF233779 mRNA. Translation: AAK71577.1.
AF233780 mRNA. Translation: AAK71578.1.
AF233781 mRNA. Translation: AAK71579.1.
AF233782 mRNA. Translation: AAK71580.1.
AF233783 mRNA. Translation: AAK71581.1.
AF233784 mRNA. Translation: AAK71582.1.
AF233785 mRNA. Translation: AAK71583.1.
AF233786 mRNA. Translation: AAK71584.1.
AF233787 mRNA. Translation: AAK71585.1.
AF233788 mRNA. Translation: AAK71586.1.
AF233789 mRNA. Translation: AAK71587.1.
AF233790 mRNA. Translation: AAK71588.1.
AF233791 mRNA. Translation: AAK71589.1.
AF233792 mRNA. Translation: AAK71590.1.
AF233793 mRNA. Translation: AAK71591.1.
AF233794 mRNA. Translation: AAK71592.1.
AF233795 mRNA. Translation: AAK71593.1.
AF233796 mRNA. Translation: AAK71594.1.
AF233797 mRNA. Translation: AAK71595.1.
AF233798 mRNA. Translation: AAK71596.1.
AF233799 mRNA. Translation: AAK71597.1.
AF233800 mRNA. Translation: AAK71598.1.
AF233801 mRNA. Translation: AAK71599.1.
AF233802 mRNA. Translation: AAK71600.1.
BC031762 mRNA. Translation: AAH31762.1.
CCDSiCCDS24551.1. [P22723-1]
CCDS24552.2. [P22723-2]
PIRiJH0316.
JH0317.
RefSeqiNP_032099.1. NM_008073.3. [P22723-1]
NP_803127.3. NM_177408.6. [P22723-2]
UniGeneiMm.5309.

3D structure databases

ProteinModelPortaliP22723.
SMRiP22723. Positions 66-474.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199808. 3 interactions.
DIPiDIP-48995N.
STRINGi10090.ENSMUSP00000063812.

Chemistry

ChEMBLiCHEMBL2094133.

PTM databases

iPTMnetiP22723.
PhosphoSiteiP22723.
SwissPalmiP22723.

Proteomic databases

MaxQBiP22723.
PaxDbiP22723.
PeptideAtlasiP22723.
PRIDEiP22723.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070725; ENSMUSP00000064739; ENSMUSG00000020436. [P22723-2]
ENSMUST00000070735; ENSMUSP00000063812; ENSMUSG00000020436. [P22723-1]
GeneIDi14406.
KEGGimmu:14406.
UCSCiuc007imb.2. mouse. [P22723-1]
uc007imc.3. mouse. [P22723-2]

Organism-specific databases

CTDi2566.
MGIiMGI:95623. Gabrg2.

Phylogenomic databases

eggNOGiKOG3642. Eukaryota.
ENOG410XNQG. LUCA.
GeneTreeiENSGT00760000119010.
HOGENOMiHOG000231337.
HOVERGENiHBG051707.
InParanoidiP22723.
KOiK05186.
OMAiYPGFTSQ.
OrthoDBiEOG7JX342.
PhylomeDBiP22723.
TreeFamiTF315453.

Enzyme and pathway databases

ReactomeiR-MMU-975298. Ligand-gated ion channel transport.
R-MMU-977441. GABA A receptor activation.

Miscellaneous databases

PROiP22723.
SOURCEiSearch...

Gene expression databases

BgeeiP22723.
ExpressionAtlasiP22723. baseline and differential.
GenevisibleiP22723. MM.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR005439. GABBAg2_rcpt.
IPR005437. GABBAg_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00253. GABAARECEPTR.
PR01620. GABAARGAMMA.
PR01622. GABAARGAMMA2.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Generation of two forms of the gamma-aminobutyric acidA receptor gamma 2-subunit in mice by alternative splicing."
    Kofuji P., Wang J.B., Moss S.J., Huganir R.L., Burt D.R.
    J. Neurochem. 56:713-715(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2L AND 2S).
  2. Sikela J.M., Shaw W.D., Khan A.S., Lin L.-H., Leidenheimer G., Siegel R.E.
    Submitted (APR-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2L).
  3. "Allelic variation in the GABA A receptor gamma2 subunit is associated with genetic susceptibility to ethanol-induced motor incoordination and hypothermia, conditioned taste aversion, and withdrawal in BXD/Ty recombinant inbred mice."
    Hood H.M., Buck K.J.
    Alcohol. Clin. Exp. Res. 24:1327-1334(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2L).
    Strain: C57BL/6J, DBA/2J and Various BXD strains.
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2L).
    Tissue: Retina.
  5. "The gamma2 subunit of GABA(A) receptors is a substrate for palmitoylation by GODZ."
    Keller C.A., Yuan X., Panzanelli P., Martin M.L., Alldred M., Sassoe-Pognetto M., Luescher B.
    J. Neurosci. 24:5881-5891(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION.
  6. "Palmitoylation regulates the clustering and cell surface stability of GABAA receptors."
    Rathenberg J., Kittler J.T., Moss S.J.
    Mol. Cell. Neurosci. 26:251-257(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION.
  7. "Histamine action on vertebrate GABAA receptors: direct channel gating and potentiation of GABA responses."
    Saras A., Gisselmann G., Vogt-Eisele A.K., Erlkamp K.S., Kletke O., Pusch H., Hatt H.
    J. Biol. Chem. 283:10470-10475(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT.

Entry informationi

Entry nameiGBRG2_MOUSE
AccessioniPrimary (citable) accession number: P22723
Secondary accession number(s): Q91V50, Q91VA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: March 27, 2002
Last modified: July 6, 2016
This is version 157 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This subunit carries the benzodiazepine binding site.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.