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Protein

Guanylate cyclase soluble subunit beta-2

Gene

Gucy1b2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Miscellaneous

There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

Cofactori

hemeBy similarityNote: Binds 1 or 2 heme groups per heterodimer.By similarity

Enzyme regulationi

Activated by nitric oxide in the presence of magnesium or manganese ions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43Iron (heme proximal ligand)By similarity1

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • guanylate cyclase activity Source: GO_Central
  • heme binding Source: InterPro
  • ion binding Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processcGMP biosynthesis
LigandGTP-binding, Heme, Iron, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate cyclase soluble subunit beta-2 (EC:4.6.1.2)
Short name:
GCS-beta-2
Gene namesi
Name:Gucy1b2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2770 Gucy1b2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000741201 – 682Guanylate cyclase soluble subunit beta-2Add BLAST682

Proteomic databases

PaxDbiP22717
PRIDEiP22717

Expressioni

Tissue specificityi

Kidney and liver.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012948

Structurei

3D structure databases

ProteinModelPortaliP22717
SMRiP22717
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini408 – 536Guanylate cyclasePROSITE-ProRule annotationAdd BLAST129

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4171 Eukaryota
COG2114 LUCA
HOVERGENiHBG051715
InParanoidiP22717
KOiK12319
PhylomeDBiP22717

Family and domain databases

Gene3Di3.30.70.1230, 1 hit
3.90.1520.10, 1 hit
InterProiView protein in InterPro
IPR001054 A/G_cyclase
IPR018297 A/G_cyclase_CS
IPR038158 H-NOX_domain_sf
IPR011644 Heme_NO-bd
IPR011645 HNOB_dom_associated
IPR024096 NO_sig/Golgi_transp_ligand-bd
IPR029787 Nucleotide_cyclase
PfamiView protein in Pfam
PF00211 Guanylate_cyc, 1 hit
PF07700 HNOB, 1 hit
PF07701 HNOBA, 1 hit
SMARTiView protein in SMART
SM00044 CYCc, 1 hit
SUPFAMiSSF111126 SSF111126, 1 hit
SSF55073 SSF55073, 1 hit
PROSITEiView protein in PROSITE
PS00452 GUANYLATE_CYCLASE_1, 1 hit
PS50125 GUANYLATE_CYCLASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P22717-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAILKLFGE YFFKFCKMSG YDRMLRTLGG NLTEFIENLD ALHSYLALSY
60 70 80 90 100
QEMNAPSFRV EEGADGAMLL HYYSDRHGLC HIVPGIIEAV AKDFFDTDVA
110 120 130 140 150
MSILDMNEEV ERTGKKEHVV FLVVQKAHRQ IRGAKASRPQ GSEDSQADQE
160 170 180 190 200
ALQGTLLRMK ERYLNIPVCP GEKSHSTAVR ASVLFGKGPL RDTFQPVYPE
210 220 230 240 250
RLWVEEEVFC DAFPFHIVFD EALRVKQAGV NIQKYVPGIL TQKFALDEYF
260 270 280 290 300
SIIHPQVTFN ISSICKFINS QFVLKTRKEM MPKARKSQPM LKLRGQMIWM
310 320 330 340 350
ESLRCMIFMC SPNVRSLQEL EESKMHLSDI APHDTTRDLI LLNQQRLAEM
360 370 380 390 400
ELSCQLEKKK EELRVLSNHL AIEKKKTETL LYAMLPEHVA NQLKEGRKVA
410 420 430 440 450
AGEFETCTIL FSDVVTFTNI CAACEPIQIV NMLNSMYSKF DRLTSVHDVY
460 470 480 490 500
KVETIGDAYM VVGGVPVPVE SHAQRVANFA LGMRISAKEV MNPVTGEPIQ
510 520 530 540 550
IRVGIHTGPV LAGVVGDKMP RYCLFGDTVN TASRMESHGL PSKVHLSPTA
560 570 580 590 600
HRALKNKGFE IVRRGEIEVK GKGKMTTYFL IQNLNATEDE IMGRPSAPAD
610 620 630 640 650
GKEVCTPGNQ VRKSPAVPRN TDHQQQVYKG DPADASNEVT LAGSPVAGRN
660 670 680
STDAVNNQPS PDETKTSVVA SGPVLSAFCV VL
Length:682
Mass (Da):76,197
Last modified:August 1, 1991 - v1
Checksum:i98C173C1A1CC7715
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M57507 mRNA Translation: AAA41207.1
PIRiA36228 OYRTB2
RefSeqiNP_001257640.1, NM_001270711.1
NP_036902.2, NM_012770.2
UniGeneiRn.10933

Genome annotation databases

GeneIDi25206
KEGGirno:25206
UCSCiRGD:2770 rat

Similar proteinsi

Entry informationi

Entry nameiGCYB2_RAT
AccessioniPrimary (citable) accession number: P22717
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: March 28, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health