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P22706

- TRAI2_ECOLX

UniProt

P22706 - TRAI2_ECOLX

Protein

Multifunctional conjugation protein TraI

Gene

traI

Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Relaxosome formation requires binding of IHF and TraY to the oriT region, which then faciliates binding of TraI relaxase. TraI forms a covalent 5'-phosphotyrosine intermediate linkage to the ssDNA. The transesterified T-strand moves from the donor cell to the recipient cell in a 5'to 3' direction, with the DNA helicase activity of TraI unwinding the DNA. DNA transfer occurs via the conjugative pore (transferosome) an intercellular junction mediated by a type IV secretion system, with TraD providing the means to link the relaxosome to the conjugative pore. The relaxase completes DNA transfer by reversing the covalent phosphotyrosine linkage and releasing the T-strand.
    TraI has also been identified as DNA helicase I. DNA. helicase I is a potent, highly processive DNA-dependent ATPase, able to unwind about 1.1 kb dsDNA per second in a 5' to 3' manner By similarity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    ATP + H2O = ADP + phosphate.

    Cofactori

    Mg2+.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei16 – 161O-(5'-phospho-DNA)-tyrosine intermediate; for relaxase activityBy similarity
    Active sitei17 – 171RelaxaseSequence Analysis
    Metal bindingi146 – 1461Magnesium; via pros nitrogen; catalyticBy similarity
    Metal bindingi157 – 1571Magnesium; via tele nitrogen; catalyticBy similarity
    Metal bindingi159 – 1591Magnesium; via tele nitrogen; catalyticBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi992 – 9998ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. DNA helicase activity Source: InterPro
    4. DNA topoisomerase type I activity Source: UniProtKB-EC
    5. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. conjugation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Helicase, Hydrolase, Isomerase, Mobility protein

    Keywords - Biological processi

    Conjugation

    Keywords - Ligandi

    ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Multifunctional conjugation protein TraI
    Including the following 2 domains:
    DNA relaxase TraI (EC:5.99.1.2)
    Alternative name(s):
    DNA nickase TraI
    Transesterase TraI
    DNA helicase I (EC:3.6.4.12)
    Gene namesi
    Name:traI
    Encoded oniPlasmid IncFII R100 (NR1)1 Publication
    Plasmid F1 Publication
    Plasmid R6-51 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 17561756Multifunctional conjugation protein TraIPRO_0000068453Add
    BLAST

    Proteomic databases

    PRIDEiP22706.

    Interactioni

    Subunit structurei

    Monomer. Part of the relaxosome, a complex composed of plasmid-encodes TraI, TraM, TraY and host-encoded IHF bound to the F plasmid origin of transfer (oriT). Directly contacts coupling protein TraD. Seems to directly contact TraM via its C-terminus.

    Structurei

    3D structure databases

    ProteinModelPortaliP22706.
    SMRiP22706. Positions 1-306.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 330330DNA relaxaseAdd
    BLAST
    Regioni950 – 1500551DNA helicase IAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1719 – 175335Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    To TraI of plasmid F.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0507.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR014059. Conjug_relaxase_N.
    IPR014129. Conjug_relaxase_TraI.
    IPR009767. DNA_helicase_TraI.
    IPR027417. P-loop_NTPase.
    IPR014862. TrwC.
    [Graphical view]
    PfamiPF07057. TraI. 1 hit.
    PF08751. TrwC. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 4 hits.
    TIGRFAMsiTIGR02686. relax_trwC. 1 hit.
    TIGR02760. TraI_TIGR. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P22706-1 [UniParc]FASTAAdd to Basket

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    MLSFSVVKSA GSAGNYYTDK DNYYVLGSMG ERWAGQGAEQ LGLQGSVDKD     50
    VFTRLLEGRL PDGADLSRMQ DGSNKHRPGY DLTFSAPKSV SMMAMLGGDK 100
    RLIDAHNQAV DFAVRQVEAL ASTRVMTDGQ SETVLTGNLV MALFNHDTSR 150
    DQDPQLHTHV VVANVTQHNG EWKTLSSDKV GKTGFSENVL ANRIAFGKIY 200
    QSELRQRVEA LGYETEVVGK HGMWEMPGVP VEAFSSRSQA IREAVGEDAS 250
    LKSRDVAALD TRKSKHHVDP EVRMAEWMQT LKETGFDIRA YRDAADQRAE 300
    IRTQAPGPAS QDGPDVQQAV TQAIAGLSER KVQFTYTDVL ARTVGILPPE 350
    NGVIERARAG IDEAISREQL IPLDREKGLF TSGIHVLDEL SVRALSRDIM 400
    KQNRVTVHPE KSVPRTAGYS DAVSVLAQDR PSLAIVSGQG GAAGQRERVA 450
    ELVMMAREQG REVQIIAADR RSQLNLKQDE RLSGELITGR RQLQEGMVFT 500
    SGSTFIVDQG EKLSLKETLT LLDGAARHNV QVLITDSGQR TGTGSALMAM 550
    KDAGVNTYRW QGGEQRPATI ISEPDRNVRY DRLAGDFAAS VKAGEESVAQ 600
    VSGVREQAIL TQAIRSELKT QGVLGHPEVT MTALSPVWLD SRSRYLRDMY 650
    RPGMVMEQWN PETRSHDRYV IDRVTAQSHS LTLRDAQGET QVVRISSLDS 700
    SWSLFRPEKM PVADGERLRV TGKIPGLRVS GGDRLQVASV SEDAMTVVVP 750
    GRAEPATLPV ADSPFTALKL ENGWVETPGH SVSDSATVFA SVTQMAMDNA 800
    TLNGLARSGR DVRLYSSLDE TRTAEKLARH PSFTVVSEQI KARAGETSLE 850
    TAISLQKTGL HTPAQQAIHL ALPVLESKNL AFSMVDLLTE AKSFAAEGTG 900
    FTELGGEINA QIKRGDLLYV DVAKGYGTGL LVSRASYEAE KSILRHILEG 950
    KEAVTPLMER VPGELMETLT SGPRAATRMI LETSDRFTVV QGYAGVGKTT 1000
    QFRAVMSAVN MLPASERPRV VGLGPTHRAV GEMRSAGVDA QTLASFLHDT 1050
    QLQQRSGETP DFSNTLFLLD ESSMVGNTDM ARAYALIAAG GGRAVASGDT 1100
    DQLQAIAPGQ PFRLQQTRSA ADVVIMKEIV RQTPELREAV YSLINRDVER 1150
    ALSGLESVKP SQVPRQEGAW APEHSVTEFS HSQEAKLAEA QQKAMLKGET 1200
    FPDVPMTLYE AIVRDYTGRT PEAREQTLIV THLNEDRRVL NSMIHDAREK 1250
    AGELGKEQVM VPVLNTANIR DGELRRLSTW ENNPDALALV DSVYHRIAGI 1300
    SKDDGLITLE DAEGNTRLIS PREAVAEGVT LYTPDKIRVG TGDRMRFTKS 1350
    DRERGYVANS VWTVTAVSGD SVTLSDGQQT RVIRPGQERA EQHIDLAYAI 1400
    TAHGAQGASE TFAIALEGTE GNRKLMAGFE SAYVALSRMK QHVQVYTDNR 1450
    QGWTDAINNA VQKGTAHDVL EPKPDREVMN AQRLFSTARE LRDVAAGRAV 1500
    LRQAGLAGGD SPARFIAPGR KYPQPYVALP AFDRNGKSAG IWLNPLTTDD 1550
    GNGLRGFSGE GRVKGSGDAQ FVALQGSRNG ESLLADNMQD GVRIARDNPD 1600
    SGVVVRIAGE GRPWNPGAIT GGRVWGDIPD SSVQPGAGNG EPVTAEVLAQ 1650
    RQAEEAIRRE TERRADEIVR KMAENKPDLP DGKTEQAVRE IAGQERDRAD 1700
    ITEREAALPE SVLRESQREQ EAVREVAREN LLQERLQQIE RDMVRDLQKE 1750
    KTLGGD 1756
    Length:1,756
    Mass (Da):191,681
    Last modified:August 1, 1991 - v1
    Checksum:iB394B666141153F3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55815 Genomic DNA. Translation: CAA39337.1.
    M38047 Genomic DNA. Translation: AAA98090.1.
    PIRiS10660. BVECAI.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55815 Genomic DNA. Translation: CAA39337.1 .
    M38047 Genomic DNA. Translation: AAA98090.1 .
    PIRi S10660. BVECAI.

    3D structure databases

    ProteinModelPortali P22706.
    SMRi P22706. Positions 1-306.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P22706.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG0507.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR014059. Conjug_relaxase_N.
    IPR014129. Conjug_relaxase_TraI.
    IPR009767. DNA_helicase_TraI.
    IPR027417. P-loop_NTPase.
    IPR014862. TrwC.
    [Graphical view ]
    Pfami PF07057. TraI. 1 hit.
    PF08751. TrwC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 4 hits.
    TIGRFAMsi TIGR02686. relax_trwC. 1 hit.
    TIGR02760. TraI_TIGR. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the promoter-distal region of the tra operon of plasmid R100, including traI (DNA helicase I) and traD genes."
      Yoshioka Y., Fujita Y., Ohtsubo E.
      J. Mol. Biol. 214:39-53(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: IncFII R100 (NR1)
    2. "Sequence and conservation of genes at the distal end of the transfer region on plasmids F and R6-5."
      Cram D.S., Loh S.M., Cheah K.C., Skurray R.A.
      Gene 104:85-90(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1747-1756.
      Plasmid: F R6-5

    Entry informationi

    Entry nameiTRAI2_ECOLX
    AccessioniPrimary (citable) accession number: P22706
    Secondary accession number(s): Q51812
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme, Plasmid

    External Data

    Dasty 3