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P22706

- TRAI2_ECOLX

UniProt

P22706 - TRAI2_ECOLX

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Protein

Multifunctional conjugation protein TraI

Gene

traI

Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Relaxosome formation requires binding of IHF and TraY to the oriT region, which then faciliates binding of TraI relaxase. TraI forms a covalent 5'-phosphotyrosine intermediate linkage to the ssDNA. The transesterified T-strand moves from the donor cell to the recipient cell in a 5'to 3' direction, with the DNA helicase activity of TraI unwinding the DNA. DNA transfer occurs via the conjugative pore (transferosome) an intercellular junction mediated by a type IV secretion system, with TraD providing the means to link the relaxosome to the conjugative pore. The relaxase completes DNA transfer by reversing the covalent phosphotyrosine linkage and releasing the T-strand.
TraI has also been identified as DNA helicase I. DNA. helicase I is a potent, highly processive DNA-dependent ATPase, able to unwind about 1.1 kb dsDNA per second in a 5' to 3' manner By similarity.By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
ATP + H2O = ADP + phosphate.

Cofactori

Mg2+.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei16 – 161O-(5'-phospho-DNA)-tyrosine intermediate; for relaxase activityBy similarity
Active sitei17 – 171RelaxaseSequence Analysis
Metal bindingi146 – 1461Magnesium; via pros nitrogen; catalyticBy similarity
Metal bindingi157 – 1571Magnesium; via tele nitrogen; catalyticBy similarity
Metal bindingi159 – 1591Magnesium; via tele nitrogen; catalyticBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi992 – 9998ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA helicase activity Source: InterPro
  4. DNA topoisomerase type I activity Source: UniProtKB-EC
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. conjugation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Isomerase, Mobility protein

Keywords - Biological processi

Conjugation

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional conjugation protein TraI
Including the following 2 domains:
DNA relaxase TraI (EC:5.99.1.2)
Alternative name(s):
DNA nickase TraI
Transesterase TraI
DNA helicase I (EC:3.6.4.12)
Gene namesi
Name:traI
Encoded oniPlasmid IncFII R100 (NR1)1 Publication
Plasmid F1 Publication
Plasmid R6-51 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17561756Multifunctional conjugation protein TraIPRO_0000068453Add
BLAST

Proteomic databases

PRIDEiP22706.

Interactioni

Subunit structurei

Monomer. Part of the relaxosome, a complex composed of plasmid-encodes TraI, TraM, TraY and host-encoded IHF bound to the F plasmid origin of transfer (oriT). Directly contacts coupling protein TraD. Seems to directly contact TraM via its C-terminus.

Structurei

3D structure databases

ProteinModelPortaliP22706.
SMRiP22706. Positions 1-306.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 330330DNA relaxaseAdd
BLAST
Regioni950 – 1500551DNA helicase IAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1719 – 175335Sequence AnalysisAdd
BLAST

Sequence similaritiesi

To TraI of plasmid F.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0507.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR014059. Conjug_relaxase_N.
IPR014129. Conjug_relaxase_TraI.
IPR009767. DNA_helicase_TraI.
IPR027417. P-loop_NTPase.
IPR014862. TrwC.
[Graphical view]
PfamiPF07057. TraI. 1 hit.
PF08751. TrwC. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
TIGRFAMsiTIGR02686. relax_trwC. 1 hit.
TIGR02760. TraI_TIGR. 1 hit.

Sequencei

Sequence statusi: Complete.

P22706-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLSFSVVKSA GSAGNYYTDK DNYYVLGSMG ERWAGQGAEQ LGLQGSVDKD
60 70 80 90 100
VFTRLLEGRL PDGADLSRMQ DGSNKHRPGY DLTFSAPKSV SMMAMLGGDK
110 120 130 140 150
RLIDAHNQAV DFAVRQVEAL ASTRVMTDGQ SETVLTGNLV MALFNHDTSR
160 170 180 190 200
DQDPQLHTHV VVANVTQHNG EWKTLSSDKV GKTGFSENVL ANRIAFGKIY
210 220 230 240 250
QSELRQRVEA LGYETEVVGK HGMWEMPGVP VEAFSSRSQA IREAVGEDAS
260 270 280 290 300
LKSRDVAALD TRKSKHHVDP EVRMAEWMQT LKETGFDIRA YRDAADQRAE
310 320 330 340 350
IRTQAPGPAS QDGPDVQQAV TQAIAGLSER KVQFTYTDVL ARTVGILPPE
360 370 380 390 400
NGVIERARAG IDEAISREQL IPLDREKGLF TSGIHVLDEL SVRALSRDIM
410 420 430 440 450
KQNRVTVHPE KSVPRTAGYS DAVSVLAQDR PSLAIVSGQG GAAGQRERVA
460 470 480 490 500
ELVMMAREQG REVQIIAADR RSQLNLKQDE RLSGELITGR RQLQEGMVFT
510 520 530 540 550
SGSTFIVDQG EKLSLKETLT LLDGAARHNV QVLITDSGQR TGTGSALMAM
560 570 580 590 600
KDAGVNTYRW QGGEQRPATI ISEPDRNVRY DRLAGDFAAS VKAGEESVAQ
610 620 630 640 650
VSGVREQAIL TQAIRSELKT QGVLGHPEVT MTALSPVWLD SRSRYLRDMY
660 670 680 690 700
RPGMVMEQWN PETRSHDRYV IDRVTAQSHS LTLRDAQGET QVVRISSLDS
710 720 730 740 750
SWSLFRPEKM PVADGERLRV TGKIPGLRVS GGDRLQVASV SEDAMTVVVP
760 770 780 790 800
GRAEPATLPV ADSPFTALKL ENGWVETPGH SVSDSATVFA SVTQMAMDNA
810 820 830 840 850
TLNGLARSGR DVRLYSSLDE TRTAEKLARH PSFTVVSEQI KARAGETSLE
860 870 880 890 900
TAISLQKTGL HTPAQQAIHL ALPVLESKNL AFSMVDLLTE AKSFAAEGTG
910 920 930 940 950
FTELGGEINA QIKRGDLLYV DVAKGYGTGL LVSRASYEAE KSILRHILEG
960 970 980 990 1000
KEAVTPLMER VPGELMETLT SGPRAATRMI LETSDRFTVV QGYAGVGKTT
1010 1020 1030 1040 1050
QFRAVMSAVN MLPASERPRV VGLGPTHRAV GEMRSAGVDA QTLASFLHDT
1060 1070 1080 1090 1100
QLQQRSGETP DFSNTLFLLD ESSMVGNTDM ARAYALIAAG GGRAVASGDT
1110 1120 1130 1140 1150
DQLQAIAPGQ PFRLQQTRSA ADVVIMKEIV RQTPELREAV YSLINRDVER
1160 1170 1180 1190 1200
ALSGLESVKP SQVPRQEGAW APEHSVTEFS HSQEAKLAEA QQKAMLKGET
1210 1220 1230 1240 1250
FPDVPMTLYE AIVRDYTGRT PEAREQTLIV THLNEDRRVL NSMIHDAREK
1260 1270 1280 1290 1300
AGELGKEQVM VPVLNTANIR DGELRRLSTW ENNPDALALV DSVYHRIAGI
1310 1320 1330 1340 1350
SKDDGLITLE DAEGNTRLIS PREAVAEGVT LYTPDKIRVG TGDRMRFTKS
1360 1370 1380 1390 1400
DRERGYVANS VWTVTAVSGD SVTLSDGQQT RVIRPGQERA EQHIDLAYAI
1410 1420 1430 1440 1450
TAHGAQGASE TFAIALEGTE GNRKLMAGFE SAYVALSRMK QHVQVYTDNR
1460 1470 1480 1490 1500
QGWTDAINNA VQKGTAHDVL EPKPDREVMN AQRLFSTARE LRDVAAGRAV
1510 1520 1530 1540 1550
LRQAGLAGGD SPARFIAPGR KYPQPYVALP AFDRNGKSAG IWLNPLTTDD
1560 1570 1580 1590 1600
GNGLRGFSGE GRVKGSGDAQ FVALQGSRNG ESLLADNMQD GVRIARDNPD
1610 1620 1630 1640 1650
SGVVVRIAGE GRPWNPGAIT GGRVWGDIPD SSVQPGAGNG EPVTAEVLAQ
1660 1670 1680 1690 1700
RQAEEAIRRE TERRADEIVR KMAENKPDLP DGKTEQAVRE IAGQERDRAD
1710 1720 1730 1740 1750
ITEREAALPE SVLRESQREQ EAVREVAREN LLQERLQQIE RDMVRDLQKE

KTLGGD
Length:1,756
Mass (Da):191,681
Last modified:August 1, 1991 - v1
Checksum:iB394B666141153F3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55815 Genomic DNA. Translation: CAA39337.1.
M38047 Genomic DNA. Translation: AAA98090.1.
PIRiS10660. BVECAI.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55815 Genomic DNA. Translation: CAA39337.1 .
M38047 Genomic DNA. Translation: AAA98090.1 .
PIRi S10660. BVECAI.

3D structure databases

ProteinModelPortali P22706.
SMRi P22706. Positions 1-306.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P22706.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0507.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR014059. Conjug_relaxase_N.
IPR014129. Conjug_relaxase_TraI.
IPR009767. DNA_helicase_TraI.
IPR027417. P-loop_NTPase.
IPR014862. TrwC.
[Graphical view ]
Pfami PF07057. TraI. 1 hit.
PF08751. TrwC. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 4 hits.
TIGRFAMsi TIGR02686. relax_trwC. 1 hit.
TIGR02760. TraI_TIGR. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the promoter-distal region of the tra operon of plasmid R100, including traI (DNA helicase I) and traD genes."
    Yoshioka Y., Fujita Y., Ohtsubo E.
    J. Mol. Biol. 214:39-53(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: IncFII R100 (NR1)
  2. "Sequence and conservation of genes at the distal end of the transfer region on plasmids F and R6-5."
    Cram D.S., Loh S.M., Cheah K.C., Skurray R.A.
    Gene 104:85-90(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1747-1756.
    Plasmid: F R6-5

Entry informationi

Entry nameiTRAI2_ECOLX
AccessioniPrimary (citable) accession number: P22706
Secondary accession number(s): Q51812
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: October 29, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme, Plasmid

External Data

Dasty 3