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P22706

- TRAI2_ECOLX

UniProt

P22706 - TRAI2_ECOLX

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Protein
Multifunctional conjugation protein TraI
Gene
traI
Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Relaxosome formation requires binding of IHF and TraY to the oriT region, which then faciliates binding of TraI relaxase. TraI forms a covalent 5'-phosphotyrosine intermediate linkage to the ssDNA. The transesterified T-strand moves from the donor cell to the recipient cell in a 5'to 3' direction, with the DNA helicase activity of TraI unwinding the DNA. DNA transfer occurs via the conjugative pore (transferosome) an intercellular junction mediated by a type IV secretion system, with TraD providing the means to link the relaxosome to the conjugative pore. The relaxase completes DNA transfer by reversing the covalent phosphotyrosine linkage and releasing the T-strand.
TraI has also been identified as DNA helicase I. DNA. helicase I is a potent, highly processive DNA-dependent ATPase, able to unwind about 1.1 kb dsDNA per second in a 5' to 3' manner By similarity.

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
ATP + H2O = ADP + phosphate.

Cofactori

Mg2+ By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei16 – 161O-(5'-phospho-DNA)-tyrosine intermediate; for relaxase activity By similarity
Active sitei17 – 171Relaxase Reviewed prediction
Metal bindingi146 – 1461Magnesium; via pros nitrogen; catalytic By similarity
Metal bindingi157 – 1571Magnesium; via tele nitrogen; catalytic By similarity
Metal bindingi159 – 1591Magnesium; via tele nitrogen; catalytic By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi992 – 9998ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA helicase activity Source: InterPro
  4. DNA topoisomerase type I activity Source: UniProtKB-EC
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. conjugation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Isomerase, Mobility protein

Keywords - Biological processi

Conjugation

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional conjugation protein TraI
Including the following 2 domains:
DNA relaxase TraI (EC:5.99.1.2)
Alternative name(s):
DNA nickase TraI
Transesterase TraI
DNA helicase I (EC:3.6.4.12)
Gene namesi
Name:traI
Encoded oniPlasmid IncFII R100 (NR1)1 Publication
Plasmid F1 Publication
Plasmid R6-51 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 17561756Multifunctional conjugation protein TraI
PRO_0000068453Add
BLAST

Proteomic databases

PRIDEiP22706.

Interactioni

Subunit structurei

Monomer. Part of the relaxosome, a complex composed of plasmid-encodes TraI, TraM, TraY and host-encoded IHF bound to the F plasmid origin of transfer (oriT). Directly contacts coupling protein TraD. Seems to directly contact TraM via its C-terminus.

Structurei

3D structure databases

ProteinModelPortaliP22706.
SMRiP22706. Positions 1-306.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 330330DNA relaxase
Add
BLAST
Regioni950 – 1500551DNA helicase I
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1719 – 175335 Reviewed prediction
Add
BLAST

Sequence similaritiesi

To TraI of plasmid F.

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0507.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR014059. Conjug_relaxase_N.
IPR014129. Conjug_relaxase_TraI.
IPR009767. DNA_helicase_TraI.
IPR027417. P-loop_NTPase.
IPR014862. TrwC.
[Graphical view]
PfamiPF07057. TraI. 1 hit.
PF08751. TrwC. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 4 hits.
TIGRFAMsiTIGR02686. relax_trwC. 1 hit.
TIGR02760. TraI_TIGR. 1 hit.

Sequencei

Sequence statusi: Complete.

P22706-1 [UniParc]FASTAAdd to Basket

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MLSFSVVKSA GSAGNYYTDK DNYYVLGSMG ERWAGQGAEQ LGLQGSVDKD     50
VFTRLLEGRL PDGADLSRMQ DGSNKHRPGY DLTFSAPKSV SMMAMLGGDK 100
RLIDAHNQAV DFAVRQVEAL ASTRVMTDGQ SETVLTGNLV MALFNHDTSR 150
DQDPQLHTHV VVANVTQHNG EWKTLSSDKV GKTGFSENVL ANRIAFGKIY 200
QSELRQRVEA LGYETEVVGK HGMWEMPGVP VEAFSSRSQA IREAVGEDAS 250
LKSRDVAALD TRKSKHHVDP EVRMAEWMQT LKETGFDIRA YRDAADQRAE 300
IRTQAPGPAS QDGPDVQQAV TQAIAGLSER KVQFTYTDVL ARTVGILPPE 350
NGVIERARAG IDEAISREQL IPLDREKGLF TSGIHVLDEL SVRALSRDIM 400
KQNRVTVHPE KSVPRTAGYS DAVSVLAQDR PSLAIVSGQG GAAGQRERVA 450
ELVMMAREQG REVQIIAADR RSQLNLKQDE RLSGELITGR RQLQEGMVFT 500
SGSTFIVDQG EKLSLKETLT LLDGAARHNV QVLITDSGQR TGTGSALMAM 550
KDAGVNTYRW QGGEQRPATI ISEPDRNVRY DRLAGDFAAS VKAGEESVAQ 600
VSGVREQAIL TQAIRSELKT QGVLGHPEVT MTALSPVWLD SRSRYLRDMY 650
RPGMVMEQWN PETRSHDRYV IDRVTAQSHS LTLRDAQGET QVVRISSLDS 700
SWSLFRPEKM PVADGERLRV TGKIPGLRVS GGDRLQVASV SEDAMTVVVP 750
GRAEPATLPV ADSPFTALKL ENGWVETPGH SVSDSATVFA SVTQMAMDNA 800
TLNGLARSGR DVRLYSSLDE TRTAEKLARH PSFTVVSEQI KARAGETSLE 850
TAISLQKTGL HTPAQQAIHL ALPVLESKNL AFSMVDLLTE AKSFAAEGTG 900
FTELGGEINA QIKRGDLLYV DVAKGYGTGL LVSRASYEAE KSILRHILEG 950
KEAVTPLMER VPGELMETLT SGPRAATRMI LETSDRFTVV QGYAGVGKTT 1000
QFRAVMSAVN MLPASERPRV VGLGPTHRAV GEMRSAGVDA QTLASFLHDT 1050
QLQQRSGETP DFSNTLFLLD ESSMVGNTDM ARAYALIAAG GGRAVASGDT 1100
DQLQAIAPGQ PFRLQQTRSA ADVVIMKEIV RQTPELREAV YSLINRDVER 1150
ALSGLESVKP SQVPRQEGAW APEHSVTEFS HSQEAKLAEA QQKAMLKGET 1200
FPDVPMTLYE AIVRDYTGRT PEAREQTLIV THLNEDRRVL NSMIHDAREK 1250
AGELGKEQVM VPVLNTANIR DGELRRLSTW ENNPDALALV DSVYHRIAGI 1300
SKDDGLITLE DAEGNTRLIS PREAVAEGVT LYTPDKIRVG TGDRMRFTKS 1350
DRERGYVANS VWTVTAVSGD SVTLSDGQQT RVIRPGQERA EQHIDLAYAI 1400
TAHGAQGASE TFAIALEGTE GNRKLMAGFE SAYVALSRMK QHVQVYTDNR 1450
QGWTDAINNA VQKGTAHDVL EPKPDREVMN AQRLFSTARE LRDVAAGRAV 1500
LRQAGLAGGD SPARFIAPGR KYPQPYVALP AFDRNGKSAG IWLNPLTTDD 1550
GNGLRGFSGE GRVKGSGDAQ FVALQGSRNG ESLLADNMQD GVRIARDNPD 1600
SGVVVRIAGE GRPWNPGAIT GGRVWGDIPD SSVQPGAGNG EPVTAEVLAQ 1650
RQAEEAIRRE TERRADEIVR KMAENKPDLP DGKTEQAVRE IAGQERDRAD 1700
ITEREAALPE SVLRESQREQ EAVREVAREN LLQERLQQIE RDMVRDLQKE 1750
KTLGGD 1756
Length:1,756
Mass (Da):191,681
Last modified:August 1, 1991 - v1
Checksum:iB394B666141153F3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55815 Genomic DNA. Translation: CAA39337.1.
M38047 Genomic DNA. Translation: AAA98090.1.
PIRiS10660. BVECAI.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55815 Genomic DNA. Translation: CAA39337.1 .
M38047 Genomic DNA. Translation: AAA98090.1 .
PIRi S10660. BVECAI.

3D structure databases

ProteinModelPortali P22706.
SMRi P22706. Positions 1-306.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P22706.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG0507.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR014059. Conjug_relaxase_N.
IPR014129. Conjug_relaxase_TraI.
IPR009767. DNA_helicase_TraI.
IPR027417. P-loop_NTPase.
IPR014862. TrwC.
[Graphical view ]
Pfami PF07057. TraI. 1 hit.
PF08751. TrwC. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 4 hits.
TIGRFAMsi TIGR02686. relax_trwC. 1 hit.
TIGR02760. TraI_TIGR. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the promoter-distal region of the tra operon of plasmid R100, including traI (DNA helicase I) and traD genes."
    Yoshioka Y., Fujita Y., Ohtsubo E.
    J. Mol. Biol. 214:39-53(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: IncFII R100 (NR1)
  2. "Sequence and conservation of genes at the distal end of the transfer region on plasmids F and R6-5."
    Cram D.S., Loh S.M., Cheah K.C., Skurray R.A.
    Gene 104:85-90(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1747-1756.
    Plasmid: F R6-5

Entry informationi

Entry nameiTRAI2_ECOLX
AccessioniPrimary (citable) accession number: P22706
Secondary accession number(s): Q51812
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme, Plasmid

External Data

Dasty 3

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