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P22706 (TRAI2_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Multifunctional conjugation protein TraI

Including the following 2 domains:

  1. DNA relaxase TraI
    EC=5.99.1.2
    Alternative name(s):
    DNA nickase TraI
    Transesterase TraI
  2. DNA helicase I
    EC=3.6.4.12
Gene names
Name:traI
Encoded onPlasmid IncFII R100 (NR1) Ref.1
Plasmid F Ref.2
Plasmid R6-5 Ref.2
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length1756 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Relaxosome formation requires binding of IHF and TraY to the oriT region, which then faciliates binding of TraI relaxase. TraI forms a covalent 5'-phosphotyrosine intermediate linkage to the ssDNA. The transesterified T-strand moves from the donor cell to the recipient cell in a 5'to 3' direction, with the DNA helicase activity of TraI unwinding the DNA. DNA transfer occurs via the conjugative pore (transferosome) an intercellular junction mediated by a type IV secretion system, with TraD providing the means to link the relaxosome to the conjugative pore. The relaxase completes DNA transfer by reversing the covalent phosphotyrosine linkage and releasing the T-strand.

TraI has also been identified as DNA helicase I. DNA. helicase I is a potent, highly processive DNA-dependent ATPase, able to unwind about 1.1 kb dsDNA per second in a 5' to 3' manner By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

ATP + H2O = ADP + phosphate.

Cofactor

Mg2+ By similarity.

Subunit structure

Monomer. Part of the relaxosome, a complex composed of plasmid-encodes TraI, TraM, TraY and host-encoded IHF bound to the F plasmid origin of transfer (oriT). Directly contacts coupling protein TraD. Seems to directly contact TraM via its C-terminus.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

To TraI of plasmid F.

Ontologies

Keywords
   Biological processConjugation
   Cellular componentCytoplasm
   DomainCoiled coil
   LigandATP-binding
DNA-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionHelicase
Hydrolase
Isomerase
Mobility protein
   Technical termMultifunctional enzyme
Plasmid
Gene Ontology (GO)
   Biological_processconjugation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA helicase activity

Inferred from electronic annotation. Source: InterPro

DNA topoisomerase type I activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 17561756Multifunctional conjugation protein TraI
PRO_0000068453

Regions

Nucleotide binding992 – 9998ATP Potential
Region1 – 330330DNA relaxase
Region950 – 1500551DNA helicase I
Coiled coil1719 – 175335 Potential

Sites

Active site161O-(5'-phospho-DNA)-tyrosine intermediate; for relaxase activity By similarity
Active site171Relaxase Potential
Metal binding1461Magnesium; via pros nitrogen; catalytic By similarity
Metal binding1571Magnesium; via tele nitrogen; catalytic By similarity
Metal binding1591Magnesium; via tele nitrogen; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
P22706 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: B394B666141153F3

FASTA1,756191,681
        10         20         30         40         50         60 
MLSFSVVKSA GSAGNYYTDK DNYYVLGSMG ERWAGQGAEQ LGLQGSVDKD VFTRLLEGRL 

        70         80         90        100        110        120 
PDGADLSRMQ DGSNKHRPGY DLTFSAPKSV SMMAMLGGDK RLIDAHNQAV DFAVRQVEAL 

       130        140        150        160        170        180 
ASTRVMTDGQ SETVLTGNLV MALFNHDTSR DQDPQLHTHV VVANVTQHNG EWKTLSSDKV 

       190        200        210        220        230        240 
GKTGFSENVL ANRIAFGKIY QSELRQRVEA LGYETEVVGK HGMWEMPGVP VEAFSSRSQA 

       250        260        270        280        290        300 
IREAVGEDAS LKSRDVAALD TRKSKHHVDP EVRMAEWMQT LKETGFDIRA YRDAADQRAE 

       310        320        330        340        350        360 
IRTQAPGPAS QDGPDVQQAV TQAIAGLSER KVQFTYTDVL ARTVGILPPE NGVIERARAG 

       370        380        390        400        410        420 
IDEAISREQL IPLDREKGLF TSGIHVLDEL SVRALSRDIM KQNRVTVHPE KSVPRTAGYS 

       430        440        450        460        470        480 
DAVSVLAQDR PSLAIVSGQG GAAGQRERVA ELVMMAREQG REVQIIAADR RSQLNLKQDE 

       490        500        510        520        530        540 
RLSGELITGR RQLQEGMVFT SGSTFIVDQG EKLSLKETLT LLDGAARHNV QVLITDSGQR 

       550        560        570        580        590        600 
TGTGSALMAM KDAGVNTYRW QGGEQRPATI ISEPDRNVRY DRLAGDFAAS VKAGEESVAQ 

       610        620        630        640        650        660 
VSGVREQAIL TQAIRSELKT QGVLGHPEVT MTALSPVWLD SRSRYLRDMY RPGMVMEQWN 

       670        680        690        700        710        720 
PETRSHDRYV IDRVTAQSHS LTLRDAQGET QVVRISSLDS SWSLFRPEKM PVADGERLRV 

       730        740        750        760        770        780 
TGKIPGLRVS GGDRLQVASV SEDAMTVVVP GRAEPATLPV ADSPFTALKL ENGWVETPGH 

       790        800        810        820        830        840 
SVSDSATVFA SVTQMAMDNA TLNGLARSGR DVRLYSSLDE TRTAEKLARH PSFTVVSEQI 

       850        860        870        880        890        900 
KARAGETSLE TAISLQKTGL HTPAQQAIHL ALPVLESKNL AFSMVDLLTE AKSFAAEGTG 

       910        920        930        940        950        960 
FTELGGEINA QIKRGDLLYV DVAKGYGTGL LVSRASYEAE KSILRHILEG KEAVTPLMER 

       970        980        990       1000       1010       1020 
VPGELMETLT SGPRAATRMI LETSDRFTVV QGYAGVGKTT QFRAVMSAVN MLPASERPRV 

      1030       1040       1050       1060       1070       1080 
VGLGPTHRAV GEMRSAGVDA QTLASFLHDT QLQQRSGETP DFSNTLFLLD ESSMVGNTDM 

      1090       1100       1110       1120       1130       1140 
ARAYALIAAG GGRAVASGDT DQLQAIAPGQ PFRLQQTRSA ADVVIMKEIV RQTPELREAV 

      1150       1160       1170       1180       1190       1200 
YSLINRDVER ALSGLESVKP SQVPRQEGAW APEHSVTEFS HSQEAKLAEA QQKAMLKGET 

      1210       1220       1230       1240       1250       1260 
FPDVPMTLYE AIVRDYTGRT PEAREQTLIV THLNEDRRVL NSMIHDAREK AGELGKEQVM 

      1270       1280       1290       1300       1310       1320 
VPVLNTANIR DGELRRLSTW ENNPDALALV DSVYHRIAGI SKDDGLITLE DAEGNTRLIS 

      1330       1340       1350       1360       1370       1380 
PREAVAEGVT LYTPDKIRVG TGDRMRFTKS DRERGYVANS VWTVTAVSGD SVTLSDGQQT 

      1390       1400       1410       1420       1430       1440 
RVIRPGQERA EQHIDLAYAI TAHGAQGASE TFAIALEGTE GNRKLMAGFE SAYVALSRMK 

      1450       1460       1470       1480       1490       1500 
QHVQVYTDNR QGWTDAINNA VQKGTAHDVL EPKPDREVMN AQRLFSTARE LRDVAAGRAV 

      1510       1520       1530       1540       1550       1560 
LRQAGLAGGD SPARFIAPGR KYPQPYVALP AFDRNGKSAG IWLNPLTTDD GNGLRGFSGE 

      1570       1580       1590       1600       1610       1620 
GRVKGSGDAQ FVALQGSRNG ESLLADNMQD GVRIARDNPD SGVVVRIAGE GRPWNPGAIT 

      1630       1640       1650       1660       1670       1680 
GGRVWGDIPD SSVQPGAGNG EPVTAEVLAQ RQAEEAIRRE TERRADEIVR KMAENKPDLP 

      1690       1700       1710       1720       1730       1740 
DGKTEQAVRE IAGQERDRAD ITEREAALPE SVLRESQREQ EAVREVAREN LLQERLQQIE 

      1750 
RDMVRDLQKE KTLGGD 

« Hide

References

[1]"Nucleotide sequence of the promoter-distal region of the tra operon of plasmid R100, including traI (DNA helicase I) and traD genes."
Yoshioka Y., Fujita Y., Ohtsubo E.
J. Mol. Biol. 214:39-53(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence and conservation of genes at the distal end of the transfer region on plasmids F and R6-5."
Cram D.S., Loh S.M., Cheah K.C., Skurray R.A.
Gene 104:85-90(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1747-1756.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55815 Genomic DNA. Translation: CAA39337.1.
M38047 Genomic DNA. Translation: AAA98090.1.
PIRBVECAI. S10660.

3D structure databases

ProteinModelPortalP22706.
SMRP22706. Positions 1-306.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP22706.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG0507.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR014059. Conjug_relaxase_N.
IPR014129. Conjug_relaxase_TraI.
IPR009767. DNA_helicase_TraI.
IPR027417. P-loop_NTPase.
IPR014862. TrwC.
[Graphical view]
PfamPF07057. TraI. 1 hit.
PF08751. TrwC. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 4 hits.
TIGRFAMsTIGR02686. relax_trwC. 1 hit.
TIGR02760. TraI_TIGR. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRAI2_ECOLX
AccessionPrimary (citable) accession number: P22706
Secondary accession number(s): Q51812
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program