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Protein

Calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type

Gene

Ca-P60A

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi304Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi305Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi307Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi309Calcium 2By similarity1
Active sitei3514-aspartylphosphate intermediateBy similarity1
Metal bindingi703MagnesiumBy similarity1
Metal bindingi707MagnesiumBy similarity1
Metal bindingi768Calcium 1By similarity1
Metal bindingi771Calcium 1By similarity1
Metal bindingi796Calcium 2By similarity1
Metal bindingi799Calcium 1By similarity1
Metal bindingi800Calcium 1By similarity1
Metal bindingi800Calcium 2By similarity1
Metal bindingi908Calcium 1By similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: FlyBase
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular calcium ion homeostasis Source: FlyBase
  • fatty acid beta-oxidation Source: FlyBase
  • flight behavior Source: FlyBase
  • heart contraction Source: FlyBase
  • intestinal stem cell homeostasis Source: FlyBase
  • lipid biosynthetic process Source: FlyBase
  • neuromuscular synaptic transmission Source: FlyBase
  • ovarian follicle cell development Source: FlyBase
  • positive regulation of calcium-transporting ATPase activity Source: FlyBase
  • positive regulation of lipid storage Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • regulation of sequestering of calcium ion Source: FlyBase
  • stabilization of membrane potential Source: FlyBase
  • stem cell differentiation Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-418359. Reduction of cytosolic Ca++ levels.
R-DME-5578775. Ion homeostasis.
R-DME-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum type (EC:3.6.3.8)
Alternative name(s):
Calcium ATPase at 60A
Calcium pump
Gene namesi
Name:Ca-P60A
ORF Names:CG3725
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0263006. Ca-P60A.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 48CytoplasmicBy similarityAdd BLAST48
Transmembranei49 – 69Helical; Name=1By similarityAdd BLAST21
Topological domaini70 – 89LumenalBy similarityAdd BLAST20
Transmembranei90 – 110Helical; Name=2By similarityAdd BLAST21
Topological domaini111 – 253CytoplasmicBy similarityAdd BLAST143
Transmembranei254 – 273Helical; Name=3By similarityAdd BLAST20
Topological domaini274 – 295LumenalBy similarityAdd BLAST22
Transmembranei296 – 313Helical; Name=4By similarityAdd BLAST18
Topological domaini314 – 757CytoplasmicBy similarityAdd BLAST444
Transmembranei758 – 777Helical; Name=5By similarityAdd BLAST20
Topological domaini778 – 787LumenalBy similarity10
Transmembranei788 – 808Helical; Name=6By similarityAdd BLAST21
Topological domaini809 – 828CytoplasmicBy similarityAdd BLAST20
Transmembranei829 – 851Helical; Name=7By similarityAdd BLAST23
Topological domaini852 – 897LumenalBy similarityAdd BLAST46
Transmembranei898 – 917Helical; Name=8By similarityAdd BLAST20
Topological domaini918 – 930CytoplasmicBy similarityAdd BLAST13
Transmembranei931 – 949Helical; Name=9By similarityAdd BLAST19
Topological domaini950 – 964LumenalBy similarityAdd BLAST15
Transmembranei965 – 985Helical; Name=10By similarityAdd BLAST21
Topological domaini986 – 1020CytoplasmicBy similarityAdd BLAST35

GO - Cellular componenti

  • endomembrane system Source: FlyBase
  • endoplasmic reticulum Source: UniProtKB
  • integral component of plasma membrane Source: GO_Central
  • lipid particle Source: FlyBase
  • nuclear envelope Source: FlyBase
  • sarcoplasmic reticulum Source: UniProtKB
  • sarcoplasmic reticulum membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462081 – 1020Calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum typeAdd BLAST1020

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei240Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP22700.
PRIDEiP22700.

PTM databases

iPTMnetiP22700.

Expressioni

Gene expression databases

BgeeiFBgn0263006.
ExpressionAtlasiP22700. baseline.
GenevisibleiP22700. DM.

Interactioni

Subunit structurei

Interacts with SclA and SclB.1 Publication

Protein-protein interaction databases

BioGridi72161. 105 interactors.
DIPiDIP-20129N.
IntActiP22700. 2 interactors.
MINTiMINT-1567799.
STRINGi7227.FBpp0072125.

Structurei

3D structure databases

ProteinModelPortaliP22700.
SMRiP22700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00850000132256.
InParanoidiP22700.
OMAiLAHWLMV.
OrthoDBiEOG091G01LE.
PhylomeDBiP22700.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005782. P-type_ATPase_IIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF08282. Hydrolase_3. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: P22700-1) [UniParc]FASTAAdd to basket
Also known as: C, D, E, F, G, H

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDGHSKTVE QSLNFFGTDP ERGLTLDQIK ANQKKYGPNE LPTEEGKSIW
60 70 80 90 100
QLVLEQFDDL LVKILLLAAI ISFVLALFEE HEETFTAFVE PLVILLILIA
110 120 130 140 150
NAVVGVWQER NAESAIEALK EYEPEMGKVV RQDKSGIQKV RAKEIVPGDL
160 170 180 190 200
VEVSVGDKIP ADIRITHIYS TTLRIDQSIL TGESVSVIKH TDAIPDPRAV
210 220 230 240 250
NQDKKNILFS GTNVAAGKAR GVVIGTGLST AIGKIRTEMS ETEEIKTPLQ
260 270 280 290 300
QKLDEFGEQL SKVISVICVA VWAINIGHFN DPAHGGSWIK GAIYYFKIAV
310 320 330 340 350
ALAVAAIPEG LPAVITTCLA LGTRRMAKKN AIVRSLPSVE TLGCTSVICS
360 370 380 390 400
DKTGTLTTNQ MSVSRMFIFD KVEGNDSSFL EFEMTGSTYE PIGEVFLNGQ
410 420 430 440 450
RIKAADYDTL QELSTICIMC NDSAIDYNEF KQAFEKVGEA TETALIVLAE
460 470 480 490 500
KLNSFSVNKS GLDRRSAAIA CRGEIETKWK KEFTLEFSRD RKSMSSYCTP
510 520 530 540 550
LKASRLGTGP KLFVKGAPEG VLERCTHARV GTTKVPLTSA LKAKILALTG
560 570 580 590 600
QYGTGRDTLR CLALAVADSP MKPDEMDLGD STKFYQYEVN LTFVGVVGML
610 620 630 640 650
DPPRKEVFDS IVRCRAAGIR VIVITGDNKA TAEAICRRIG VFAEDEDTTG
660 670 680 690 700
KSYSGREFDD LSPTEQKAAV ARSRLFSRVE PQHKSKIVEF LQSMNEISAM
710 720 730 740 750
TGDGVNDAPA LKKAEIGIAM GSGTAVAKSA AEMVLADDNF SSIVSAVEEG
760 770 780 790 800
RAIYNNMKQF IRYLISSNIG EVVSIFLTAA LGLPEALIPV QLLWVNLVTD
810 820 830 840 850
GLPATALGFN PPDLDIMEKP PRKADEGLIS GWLFFRYMAI GFYVGAATVG
860 870 880 890 900
AAAWWFVFSD EGPKLSYWQL THHLSCLGGG DEFKGVDCKI FSDPHAMTMA
910 920 930 940 950
LSVLVTIEML NAMNSLSENQ SLITMPPWCN LWLIGSMALS FTLHFVILYV
960 970 980 990 1000
DVLSTVFQVT PLSAEEWITV MKFSIPVVLL DETLKFVARK IADGESPIYK
1010 1020
MHGIVLMWAV FFGLLYAMML
Note: No experimental confirmation available.
Length:1,020
Mass (Da):111,701
Last modified:December 1, 2000 - v2
Checksum:i6A62D350BF316984
GO
Isoform A (identifier: P22700-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     994-1020: GESPIYKMHGIVLMWAVFFGLLYAMML → VPDVVVDRM

Show »
Length:1,002
Mass (Da):109,611
Checksum:i463400C1967BD7D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti158K → R in AAL13694 (PubMed:12537569).Curated1
Sequence conflicti302L → V in AAB00735 (PubMed:2148477).Curated1
Sequence conflicti357T → L in CAA35505 (PubMed:2557235).Curated1
Sequence conflicti495S → P in AAL13694 (PubMed:12537569).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010297994 – 1020GESPI…YAMML → VPDVVVDRM in isoform A. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62892 mRNA. Translation: AAB00735.1.
AE013599 Genomic DNA. Translation: AAF47101.1.
AE013599 Genomic DNA. Translation: AAF47102.1.
AE013599 Genomic DNA. Translation: AAF47103.1.
AE013599 Genomic DNA. Translation: AAM68278.1.
AE013599 Genomic DNA. Translation: AAM68279.1.
AE013599 Genomic DNA. Translation: AAM68280.1.
AE013599 Genomic DNA. Translation: AAM68281.1.
AY058465 mRNA. Translation: AAL13694.1.
X17472 mRNA. Translation: CAA35505.1.
PIRiA36691.
RefSeqiNP_476832.1. NM_057484.3. [P22700-2]
NP_726381.1. NM_166633.2. [P22700-1]
NP_726382.1. NM_166634.2. [P22700-1]
NP_726383.1. NM_166635.2. [P22700-1]
NP_726384.1. NM_166636.2. [P22700-1]
NP_726385.1. NM_166637.2. [P22700-1]
NP_726386.1. NM_166638.2. [P22700-1]
NP_726387.1. NM_166639.2. [P22700-1]
UniGeneiDm.3812.

Genome annotation databases

EnsemblMetazoaiFBtr0072211; FBpp0072120; FBgn0263006. [P22700-1]
FBtr0072212; FBpp0072121; FBgn0263006. [P22700-1]
FBtr0072213; FBpp0072122; FBgn0263006. [P22700-1]
FBtr0072214; FBpp0072123; FBgn0263006. [P22700-1]
FBtr0072216; FBpp0072125; FBgn0263006. [P22700-1]
FBtr0072217; FBpp0072126; FBgn0263006. [P22700-1]
FBtr0072218; FBpp0072127; FBgn0263006. [P22700-1]
GeneIDi49297.
KEGGidme:Dmel_CG3725.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62892 mRNA. Translation: AAB00735.1.
AE013599 Genomic DNA. Translation: AAF47101.1.
AE013599 Genomic DNA. Translation: AAF47102.1.
AE013599 Genomic DNA. Translation: AAF47103.1.
AE013599 Genomic DNA. Translation: AAM68278.1.
AE013599 Genomic DNA. Translation: AAM68279.1.
AE013599 Genomic DNA. Translation: AAM68280.1.
AE013599 Genomic DNA. Translation: AAM68281.1.
AY058465 mRNA. Translation: AAL13694.1.
X17472 mRNA. Translation: CAA35505.1.
PIRiA36691.
RefSeqiNP_476832.1. NM_057484.3. [P22700-2]
NP_726381.1. NM_166633.2. [P22700-1]
NP_726382.1. NM_166634.2. [P22700-1]
NP_726383.1. NM_166635.2. [P22700-1]
NP_726384.1. NM_166636.2. [P22700-1]
NP_726385.1. NM_166637.2. [P22700-1]
NP_726386.1. NM_166638.2. [P22700-1]
NP_726387.1. NM_166639.2. [P22700-1]
UniGeneiDm.3812.

3D structure databases

ProteinModelPortaliP22700.
SMRiP22700.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi72161. 105 interactors.
DIPiDIP-20129N.
IntActiP22700. 2 interactors.
MINTiMINT-1567799.
STRINGi7227.FBpp0072125.

PTM databases

iPTMnetiP22700.

Proteomic databases

PaxDbiP22700.
PRIDEiP22700.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072211; FBpp0072120; FBgn0263006. [P22700-1]
FBtr0072212; FBpp0072121; FBgn0263006. [P22700-1]
FBtr0072213; FBpp0072122; FBgn0263006. [P22700-1]
FBtr0072214; FBpp0072123; FBgn0263006. [P22700-1]
FBtr0072216; FBpp0072125; FBgn0263006. [P22700-1]
FBtr0072217; FBpp0072126; FBgn0263006. [P22700-1]
FBtr0072218; FBpp0072127; FBgn0263006. [P22700-1]
GeneIDi49297.
KEGGidme:Dmel_CG3725.

Organism-specific databases

CTDi49297.
FlyBaseiFBgn0263006. Ca-P60A.

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00850000132256.
InParanoidiP22700.
OMAiLAHWLMV.
OrthoDBiEOG091G01LE.
PhylomeDBiP22700.

Enzyme and pathway databases

ReactomeiR-DME-418359. Reduction of cytosolic Ca++ levels.
R-DME-5578775. Ion homeostasis.
R-DME-936837. Ion transport by P-type ATPases.

Miscellaneous databases

GenomeRNAii49297.
PROiP22700.

Gene expression databases

BgeeiFBgn0263006.
ExpressionAtlasiP22700. baseline.
GenevisibleiP22700. DM.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005782. P-type_ATPase_IIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF08282. Hydrolase_3. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01116. ATPase-IIA1_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATC1_DROME
AccessioniPrimary (citable) accession number: P22700
Secondary accession number(s): A4UZU0
, Q2MGN2, Q95TX1, Q9W1G2, Q9W1G3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 169 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.