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P22699 (GUN6_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase

EC=3.2.1.4
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Spore germination protein 270-6
Gene names
Name:celA
ORF Names:DDB_G0271134
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length705 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May digest the spore cell wall during germination, to release the enclosed amoeba. Ref.4

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Developmental stage

Found predominantly during early stages of spore germination.

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 705682Endoglucanase
PRO_0000007953

Regions

Region463 – 55290Pro/Thr repeats ("hinge") (Pro/Thr box)

Sites

Active site3901 By similarity
Active site4281 By similarity
Active site4371 By similarity

Amino acid modifications

Glycosylation751N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P22699 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: F0457BDC07576A15

FASTA70575,911
        10         20         30         40         50         60 
MKILKNCILL IIFGLLSTQL INADTDYCSL LENALMFYKM NRAGRLPDND IPWRGNSALN 

        70         80         90        100        110        120 
DASPNSAKDA NGDGNLSGGY FDAGDGVKFG LPMAYSMTML GWSFIEYESN IAQCGLTSLY 

       130        140        150        160        170        180 
LDTIKYGTDW LIAAHTADNE FAGQVGDGNV DHSWWGPPED MTMARPTYML TTEAPGTEIA 

       190        200        210        220        230        240 
MEAASALAAA SIAFKSSNPT YAATCLAHAK TLHNFGYTYR GVYSDSITNA QAFYNSWSGY 

       250        260        270        280        290        300 
KDDLVWGSIW LYKATQDSDY LTKAVADYAS GGVGGMAQGN SHDWDNKAPG CCLLLSKLVP 

       310        320        330        340        350        360 
TTSTYKTDFE GWLNYWLPGG GVTYTPGGLA WIRQWGPARY AATAAFLGSL AGTEKGTDFT 

       370        380        390        400        410        420 
QKQVDYLIGN NPNQQSFVVG MGPNYPINPH HRAAHHSTTN DINNPVNNLY LLKGALVGGP 

       430        440        450        460        470        480 
GSNDEYTDDR TDYISNEVAT DYNAGFVGAL ASLVNPSSTS VPTTTPTVTE TPTETPTETP 

       490        500        510        520        530        540 
TETPTETPTE TPTETPTETP TETPTETPTE TPTETPTETP TETPTETPTE TPTETPTETV 

       550        560        570        580        590        600 
TPTPTVTPTE TPSSGESLSI YKSGLKNDFQ DWSWGEHSLT DTTNVESGET NSISFTPKAY 

       610        620        630        640        650        660 
GAVFLGCFEC IDTDTYNNIE FDINGGSSGA QLLRITVVKN SKSVGSKLIT DLNGGTPIEA 

       670        680        690        700 
NSWTKIKASF IDDFKVSGKV DGIWIQDIKG DTQSTVYISN IIATA 

« Hide

References

« Hide 'large scale' references
[1]"A shared internal threonine-glutamic acid-threonine-proline repeat defines a family of Dictyostelium discoideum spore germination specific proteins."
Giorda R., Ohmachi T., Shaw D.R., Ennis H.L.
Biochemistry 29:7264-7269(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[4]"A Dictyostelium discoideum cellulase is a member of a spore germination-specific gene family."
Blume J.E., Ennis H.L.
J. Biol. Chem. 266:15432-15437(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M33861 Genomic DNA. Translation: AAA52077.1.
AAFI02000006 Genomic DNA. Translation: EAL71697.1.
PIRA35621.
RefSeqXP_645627.1. XM_640535.1.

3D structure databases

ProteinModelPortalP22699.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0215351.

Protein family/group databases

CAZyCBM8. Carbohydrate-Binding Module Family 8.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0215351; DDB0215351; DDB_G0271134.
GeneID8617819.
KEGGddi:DDB_G0271134.

Organism-specific databases

dictyBaseDDB_G0271134. celA.

Phylogenomic databases

eggNOGNOG05134.
OMAEYESNIA.
PhylomeDBP22699.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN6_DICDI
AccessionPrimary (citable) accession number: P22699
Secondary accession number(s): Q55BI5
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase