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P22699

- GUN6_DICDI

UniProt

P22699 - GUN6_DICDI

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Protein

Endoglucanase

Gene

celA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

May digest the spore cell wall during germination, to release the enclosed amoeba.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei390 – 3901By similarity
Active sitei428 – 4281By similarity
Active sitei437 – 4371By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
  2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation, Sporulation

Protein family/group databases

CAZyiCBM8. Carbohydrate-Binding Module Family 8.
GH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Spore germination protein 270-6
Gene namesi
Name:celA
ORF Names:DDB_G0271134
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0271134. celA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 705682EndoglucanasePRO_0000007953Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Developmental stagei

Found predominantly during early stages of spore germination.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0215351.

Structurei

3D structure databases

ProteinModelPortaliP22699.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni463 – 55290Pro/Thr repeats ("hinge") (Pro/Thr box)Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG05134.
InParanoidiP22699.
KOiK01179.
OMAiEYESNIA.
PhylomeDBiP22699.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22699-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKILKNCILL IIFGLLSTQL INADTDYCSL LENALMFYKM NRAGRLPDND
60 70 80 90 100
IPWRGNSALN DASPNSAKDA NGDGNLSGGY FDAGDGVKFG LPMAYSMTML
110 120 130 140 150
GWSFIEYESN IAQCGLTSLY LDTIKYGTDW LIAAHTADNE FAGQVGDGNV
160 170 180 190 200
DHSWWGPPED MTMARPTYML TTEAPGTEIA MEAASALAAA SIAFKSSNPT
210 220 230 240 250
YAATCLAHAK TLHNFGYTYR GVYSDSITNA QAFYNSWSGY KDDLVWGSIW
260 270 280 290 300
LYKATQDSDY LTKAVADYAS GGVGGMAQGN SHDWDNKAPG CCLLLSKLVP
310 320 330 340 350
TTSTYKTDFE GWLNYWLPGG GVTYTPGGLA WIRQWGPARY AATAAFLGSL
360 370 380 390 400
AGTEKGTDFT QKQVDYLIGN NPNQQSFVVG MGPNYPINPH HRAAHHSTTN
410 420 430 440 450
DINNPVNNLY LLKGALVGGP GSNDEYTDDR TDYISNEVAT DYNAGFVGAL
460 470 480 490 500
ASLVNPSSTS VPTTTPTVTE TPTETPTETP TETPTETPTE TPTETPTETP
510 520 530 540 550
TETPTETPTE TPTETPTETP TETPTETPTE TPTETPTETV TPTPTVTPTE
560 570 580 590 600
TPSSGESLSI YKSGLKNDFQ DWSWGEHSLT DTTNVESGET NSISFTPKAY
610 620 630 640 650
GAVFLGCFEC IDTDTYNNIE FDINGGSSGA QLLRITVVKN SKSVGSKLIT
660 670 680 690 700
DLNGGTPIEA NSWTKIKASF IDDFKVSGKV DGIWIQDIKG DTQSTVYISN

IIATA
Length:705
Mass (Da):75,911
Last modified:August 1, 1991 - v1
Checksum:iF0457BDC07576A15
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33861 Genomic DNA. Translation: AAA52077.1.
AAFI02000006 Genomic DNA. Translation: EAL71697.1.
PIRiA35621.
RefSeqiXP_645627.1. XM_640535.1.

Genome annotation databases

EnsemblProtistsiDDB0215351; DDB0215351; DDB_G0271134.
GeneIDi8617819.
KEGGiddi:DDB_G0271134.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33861 Genomic DNA. Translation: AAA52077.1 .
AAFI02000006 Genomic DNA. Translation: EAL71697.1 .
PIRi A35621.
RefSeqi XP_645627.1. XM_640535.1.

3D structure databases

ProteinModelPortali P22699.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0215351.

Protein family/group databases

CAZyi CBM8. Carbohydrate-Binding Module Family 8.
GH9. Glycoside Hydrolase Family 9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0215351 ; DDB0215351 ; DDB_G0271134 .
GeneIDi 8617819.
KEGGi ddi:DDB_G0271134.

Organism-specific databases

dictyBasei DDB_G0271134. celA.

Phylogenomic databases

eggNOGi NOG05134.
InParanoidi P22699.
KOi K01179.
OMAi EYESNIA.
PhylomeDBi P22699.

Family and domain databases

Gene3Di 1.50.10.10. 1 hit.
InterProi IPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view ]
Pfami PF00759. Glyco_hydro_9. 1 hit.
[Graphical view ]
SUPFAMi SSF48208. SSF48208. 1 hit.
PROSITEi PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A shared internal threonine-glutamic acid-threonine-proline repeat defines a family of Dictyostelium discoideum spore germination specific proteins."
    Giorda R., Ohmachi T., Shaw D.R., Ennis H.L.
    Biochemistry 29:7264-7269(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  4. "A Dictyostelium discoideum cellulase is a member of a spore germination-specific gene family."
    Blume J.E., Ennis H.L.
    J. Biol. Chem. 266:15432-15437(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGUN6_DICDI
AccessioniPrimary (citable) accession number: P22699
Secondary accession number(s): Q55BI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: October 29, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3