P22699 (GUN6_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase
EC=3.2.1.4

Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Spore germination protein 270-6

Gene names

Name:	celA
ORF Names:	DDB_G0271134

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	705 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May digest the spore cell wall during germination, to release the enclosed amoeba. Ref.4
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Developmental stage	Found predominantly during early stages of spore germination.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation Sporulation
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 705	682	Endoglucanase		PRO_0000007953
Regions
	Region	463 – 552	90	Pro/Thr repeats ("hinge") (Pro/Thr box)
Sites
	Active site	390	1	By similarity
	Active site	428	1	By similarity
	Active site	437	1	By similarity
Amino acid modifications
	Glycosylation	75	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P22699 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: F0457BDC07576A15	FASTA	705	75,911
	10 20 30 40 50 60 MKILKNCILL IIFGLLSTQL INADTDYCSL LENALMFYKM NRAGRLPDND IPWRGNSALN 70 80 90 100 110 120 DASPNSAKDA NGDGNLSGGY FDAGDGVKFG LPMAYSMTML GWSFIEYESN IAQCGLTSLY 130 140 150 160 170 180 LDTIKYGTDW LIAAHTADNE FAGQVGDGNV DHSWWGPPED MTMARPTYML TTEAPGTEIA 190 200 210 220 230 240 MEAASALAAA SIAFKSSNPT YAATCLAHAK TLHNFGYTYR GVYSDSITNA QAFYNSWSGY 250 260 270 280 290 300 KDDLVWGSIW LYKATQDSDY LTKAVADYAS GGVGGMAQGN SHDWDNKAPG CCLLLSKLVP 310 320 330 340 350 360 TTSTYKTDFE GWLNYWLPGG GVTYTPGGLA WIRQWGPARY AATAAFLGSL AGTEKGTDFT 370 380 390 400 410 420 QKQVDYLIGN NPNQQSFVVG MGPNYPINPH HRAAHHSTTN DINNPVNNLY LLKGALVGGP 430 440 450 460 470 480 GSNDEYTDDR TDYISNEVAT DYNAGFVGAL ASLVNPSSTS VPTTTPTVTE TPTETPTETP 490 500 510 520 530 540 TETPTETPTE TPTETPTETP TETPTETPTE TPTETPTETP TETPTETPTE TPTETPTETV 550 560 570 580 590 600 TPTPTVTPTE TPSSGESLSI YKSGLKNDFQ DWSWGEHSLT DTTNVESGET NSISFTPKAY 610 620 630 640 650 660 GAVFLGCFEC IDTDTYNNIE FDINGGSSGA QLLRITVVKN SKSVGSKLIT DLNGGTPIEA 670 680 690 700 NSWTKIKASF IDDFKVSGKV DGIWIQDIKG DTQSTVYISN IIATA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A shared internal threonine-glutamic acid-threonine-proline repeat defines a family of Dictyostelium discoideum spore germination specific proteins." Giorda R., Ohmachi T., Shaw D.R., Ennis H.L. Biochemistry 29:7264-7269(1990) [PubMed: 1976383] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"A Dictyostelium discoideum cellulase is a member of a spore germination-specific gene family." Blume J.E., Ennis H.L. J. Biol. Chem. 266:15432-15437(1991) [PubMed: 1869562] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M33861 Genomic DNA. Translation: AAA52077.1. AAFI02000006 Genomic DNA. Translation: EAL71697.1.
PIR	A35621.
RefSeq	XP_645627.1. XM_640535.1.
3D structure databases
ProteinModelPortal	P22699.
ModBase	Search...
Protein-protein interaction databases
STRING	P22699.
Protein family/group databases
CAZy	CBM8. Carbohydrate-Binding Module Family 8. GH9. Glycoside Hydrolase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0215351; DDB0215351; DDB_G0271134.
GeneID	8617819.
GenomeReviews	Gene locus celA in contig CM000151_GR.
KEGG	ddi:DDB_G0271134.
Organism-specific databases
dictyBase	DDB_G0271134. celA.
Phylogenomic databases
eggNOG	euNOG08940.
GeneTree	EPrGT00050000001978.
HOGENOM	HBG746347.
OMA	ITINSAN.
ProtClustDB	CLSZ2430121.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
KO	K01179.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN6_DICDI

Accession

Primary (citable) accession number: P22699
Secondary accession number(s): Q55BI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	November 16, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)