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P22699

- GUN6_DICDI

UniProt

P22699 - GUN6_DICDI

Protein

Endoglucanase

Gene

celA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    May digest the spore cell wall during germination, to release the enclosed amoeba.1 Publication

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei390 – 3901By similarity
    Active sitei428 – 4281By similarity
    Active sitei437 – 4371By similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW
    2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation, Sporulation

    Protein family/group databases

    CAZyiCBM8. Carbohydrate-Binding Module Family 8.
    GH9. Glycoside Hydrolase Family 9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase (EC:3.2.1.4)
    Alternative name(s):
    Cellulase
    Endo-1,4-beta-glucanase
    Spore germination protein 270-6
    Gene namesi
    Name:celA
    ORF Names:DDB_G0271134
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 2, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0271134. celA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 705682EndoglucanasePRO_0000007953Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Developmental stagei

    Found predominantly during early stages of spore germination.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0215351.

    Structurei

    3D structure databases

    ProteinModelPortaliP22699.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni463 – 55290Pro/Thr repeats ("hinge") (Pro/Thr box)Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG05134.
    KOiK01179.
    OMAiEYESNIA.
    PhylomeDBiP22699.

    Family and domain databases

    Gene3Di1.50.10.10. 1 hit.
    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view]
    PfamiPF00759. Glyco_hydro_9. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.
    PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22699-1 [UniParc]FASTAAdd to Basket

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    MKILKNCILL IIFGLLSTQL INADTDYCSL LENALMFYKM NRAGRLPDND    50
    IPWRGNSALN DASPNSAKDA NGDGNLSGGY FDAGDGVKFG LPMAYSMTML 100
    GWSFIEYESN IAQCGLTSLY LDTIKYGTDW LIAAHTADNE FAGQVGDGNV 150
    DHSWWGPPED MTMARPTYML TTEAPGTEIA MEAASALAAA SIAFKSSNPT 200
    YAATCLAHAK TLHNFGYTYR GVYSDSITNA QAFYNSWSGY KDDLVWGSIW 250
    LYKATQDSDY LTKAVADYAS GGVGGMAQGN SHDWDNKAPG CCLLLSKLVP 300
    TTSTYKTDFE GWLNYWLPGG GVTYTPGGLA WIRQWGPARY AATAAFLGSL 350
    AGTEKGTDFT QKQVDYLIGN NPNQQSFVVG MGPNYPINPH HRAAHHSTTN 400
    DINNPVNNLY LLKGALVGGP GSNDEYTDDR TDYISNEVAT DYNAGFVGAL 450
    ASLVNPSSTS VPTTTPTVTE TPTETPTETP TETPTETPTE TPTETPTETP 500
    TETPTETPTE TPTETPTETP TETPTETPTE TPTETPTETV TPTPTVTPTE 550
    TPSSGESLSI YKSGLKNDFQ DWSWGEHSLT DTTNVESGET NSISFTPKAY 600
    GAVFLGCFEC IDTDTYNNIE FDINGGSSGA QLLRITVVKN SKSVGSKLIT 650
    DLNGGTPIEA NSWTKIKASF IDDFKVSGKV DGIWIQDIKG DTQSTVYISN 700
    IIATA 705
    Length:705
    Mass (Da):75,911
    Last modified:August 1, 1991 - v1
    Checksum:iF0457BDC07576A15
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33861 Genomic DNA. Translation: AAA52077.1.
    AAFI02000006 Genomic DNA. Translation: EAL71697.1.
    PIRiA35621.
    RefSeqiXP_645627.1. XM_640535.1.

    Genome annotation databases

    EnsemblProtistsiDDB0215351; DDB0215351; DDB_G0271134.
    GeneIDi8617819.
    KEGGiddi:DDB_G0271134.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33861 Genomic DNA. Translation: AAA52077.1 .
    AAFI02000006 Genomic DNA. Translation: EAL71697.1 .
    PIRi A35621.
    RefSeqi XP_645627.1. XM_640535.1.

    3D structure databases

    ProteinModelPortali P22699.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0215351.

    Protein family/group databases

    CAZyi CBM8. Carbohydrate-Binding Module Family 8.
    GH9. Glycoside Hydrolase Family 9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0215351 ; DDB0215351 ; DDB_G0271134 .
    GeneIDi 8617819.
    KEGGi ddi:DDB_G0271134.

    Organism-specific databases

    dictyBasei DDB_G0271134. celA.

    Phylogenomic databases

    eggNOGi NOG05134.
    KOi K01179.
    OMAi EYESNIA.
    PhylomeDBi P22699.

    Family and domain databases

    Gene3Di 1.50.10.10. 1 hit.
    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR012341. 6hp_glycosidase.
    IPR001701. Glyco_hydro_9.
    IPR018221. Glyco_hydro_9_AS.
    [Graphical view ]
    Pfami PF00759. Glyco_hydro_9. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    PROSITEi PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
    PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A shared internal threonine-glutamic acid-threonine-proline repeat defines a family of Dictyostelium discoideum spore germination specific proteins."
      Giorda R., Ohmachi T., Shaw D.R., Ennis H.L.
      Biochemistry 29:7264-7269(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    4. "A Dictyostelium discoideum cellulase is a member of a spore germination-specific gene family."
      Blume J.E., Ennis H.L.
      J. Biol. Chem. 266:15432-15437(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiGUN6_DICDI
    AccessioniPrimary (citable) accession number: P22699
    Secondary accession number(s): Q55BI5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3