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Protein

Endoglucanase

Gene

celA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May digest the spore cell wall during germination, to release the enclosed amoeba.1 Publication

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei390By similarity1
Active sitei428By similarity1
Active sitei437By similarity1

GO - Molecular functioni

  • cellulase activity Source: dictyBase
  • cellulose binding Source: dictyBase

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation, Sporulation

Protein family/group databases

CAZyiCBM8. Carbohydrate-Binding Module Family 8.
GH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase (EC:3.2.1.4)
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
Spore germination protein 270-6
Gene namesi
Name:celA
ORF Names:DDB_G0271134
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 2, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0271134. celA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000000795324 – 705EndoglucanaseAdd BLAST682

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi75N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP22699.

Expressioni

Developmental stagei

Found predominantly during early stages of spore germination.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0215351.

Structurei

3D structure databases

ProteinModelPortaliP22699.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni463 – 552Pro/Thr repeats ("hinge") (Pro/Thr box)Add BLAST90

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IDXW. Eukaryota.
ENOG410XPC9. LUCA.
InParanoidiP22699.
KOiK01179.
OMAiPIEANSW.
PhylomeDBiP22699.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR033126. Glyco_hydro_9_Asp/Glu_AS.
IPR018221. Glyco_hydro_9_His_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22699-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKILKNCILL IIFGLLSTQL INADTDYCSL LENALMFYKM NRAGRLPDND
60 70 80 90 100
IPWRGNSALN DASPNSAKDA NGDGNLSGGY FDAGDGVKFG LPMAYSMTML
110 120 130 140 150
GWSFIEYESN IAQCGLTSLY LDTIKYGTDW LIAAHTADNE FAGQVGDGNV
160 170 180 190 200
DHSWWGPPED MTMARPTYML TTEAPGTEIA MEAASALAAA SIAFKSSNPT
210 220 230 240 250
YAATCLAHAK TLHNFGYTYR GVYSDSITNA QAFYNSWSGY KDDLVWGSIW
260 270 280 290 300
LYKATQDSDY LTKAVADYAS GGVGGMAQGN SHDWDNKAPG CCLLLSKLVP
310 320 330 340 350
TTSTYKTDFE GWLNYWLPGG GVTYTPGGLA WIRQWGPARY AATAAFLGSL
360 370 380 390 400
AGTEKGTDFT QKQVDYLIGN NPNQQSFVVG MGPNYPINPH HRAAHHSTTN
410 420 430 440 450
DINNPVNNLY LLKGALVGGP GSNDEYTDDR TDYISNEVAT DYNAGFVGAL
460 470 480 490 500
ASLVNPSSTS VPTTTPTVTE TPTETPTETP TETPTETPTE TPTETPTETP
510 520 530 540 550
TETPTETPTE TPTETPTETP TETPTETPTE TPTETPTETV TPTPTVTPTE
560 570 580 590 600
TPSSGESLSI YKSGLKNDFQ DWSWGEHSLT DTTNVESGET NSISFTPKAY
610 620 630 640 650
GAVFLGCFEC IDTDTYNNIE FDINGGSSGA QLLRITVVKN SKSVGSKLIT
660 670 680 690 700
DLNGGTPIEA NSWTKIKASF IDDFKVSGKV DGIWIQDIKG DTQSTVYISN

IIATA
Length:705
Mass (Da):75,911
Last modified:August 1, 1991 - v1
Checksum:iF0457BDC07576A15
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33861 Genomic DNA. Translation: AAA52077.1.
AAFI02000006 Genomic DNA. Translation: EAL71697.1.
PIRiA35621.
RefSeqiXP_645627.1. XM_640535.1.

Genome annotation databases

EnsemblProtistsiEAL71697; EAL71697; DDB_G0271134.
GeneIDi8617819.
KEGGiddi:DDB_G0271134.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33861 Genomic DNA. Translation: AAA52077.1.
AAFI02000006 Genomic DNA. Translation: EAL71697.1.
PIRiA35621.
RefSeqiXP_645627.1. XM_640535.1.

3D structure databases

ProteinModelPortaliP22699.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0215351.

Protein family/group databases

CAZyiCBM8. Carbohydrate-Binding Module Family 8.
GH9. Glycoside Hydrolase Family 9.

Proteomic databases

PaxDbiP22699.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL71697; EAL71697; DDB_G0271134.
GeneIDi8617819.
KEGGiddi:DDB_G0271134.

Organism-specific databases

dictyBaseiDDB_G0271134. celA.

Phylogenomic databases

eggNOGiENOG410IDXW. Eukaryota.
ENOG410XPC9. LUCA.
InParanoidiP22699.
KOiK01179.
OMAiPIEANSW.
PhylomeDBiP22699.

Miscellaneous databases

PROiP22699.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR033126. Glyco_hydro_9_Asp/Glu_AS.
IPR018221. Glyco_hydro_9_His_AS.
[Graphical view]
PfamiPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUN6_DICDI
AccessioniPrimary (citable) accession number: P22699
Secondary accession number(s): Q55BI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.