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Protein

Cholesterol 7-alpha-monooxygenase

Gene

CYP7A1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.1 Publication

Catalytic activityi

Cholesterol + [reduced NADPH--hemoprotein reductase] + O2 = 7-alpha-hydroxycholesterol + [oxidized NADPH--hemoprotein reductase] + H2O.1 Publication

Cofactori

heme1 Publication

Pathwayi: bile acid biosynthesis

This protein is involved in the pathway bile acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway bile acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi444Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

  • bile acid biosynthetic process Source: UniProtKB
  • cellular response to cholesterol Source: UniProtKB
  • cellular response to glucose stimulus Source: UniProtKB
  • cholesterol catabolic process Source: UniProtKB
  • cholesterol homeostasis Source: UniProtKB
  • regulation of bile acid biosynthetic process Source: UniProtKB
  • sterol metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:HS09659-MONOMER.
ZFISH:HS09659-MONOMER.
BRENDAi1.14.13.17. 2681.
ReactomeiR-HSA-192105. Synthesis of bile acids and bile salts.
R-HSA-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-HSA-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
R-HSA-1989781. PPARA activates gene expression.
R-HSA-211976. Endogenous sterols.
SABIO-RKP22680.
UniPathwayiUPA00221.

Chemistry databases

SwissLipidsiSLP:000001200.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol 7-alpha-monooxygenase (EC:1.14.14.231 Publication)
Alternative name(s):
CYPVII
Cholesterol 7-alpha-hydroxylase
Cytochrome P450 7A1
Gene namesi
Name:CYP7A1
Synonyms:CYP7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:2651. CYP7A1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

DisGeNETi1581.
MalaCardsiCYP7A1.
OpenTargetsiENSG00000167910.
Orphaneti209902. Hypercholesterolemia due to cholesterol 7alpha-hydroxylase deficiency.
PharmGKBiPA132.

Polymorphism and mutation databases

BioMutaiCYP7A1.
DMDMi544084.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519011 – 504Cholesterol 7-alpha-monooxygenaseAdd BLAST504

Proteomic databases

EPDiP22680.
PaxDbiP22680.
PRIDEiP22680.

PTM databases

iPTMnetiP22680.
PhosphoSitePlusiP22680.

Expressioni

Tissue specificityi

Detected in liver.1 Publication

Inductioni

Up-regulated by glucose and by cholestyramine. Down-regulated by chenodeoxycholic acid.2 Publications

Gene expression databases

BgeeiENSG00000167910.
CleanExiHS_CYP7A1.
GenevisibleiP22680. HS.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000301645.

Structurei

Secondary structure

1504
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 41Combined sources8
Turni42 – 45Combined sources4
Helixi46 – 48Combined sources3
Helixi52 – 63Combined sources12
Beta strandi65 – 71Combined sources7
Beta strandi74 – 79Combined sources6
Helixi82 – 84Combined sources3
Helixi85 – 89Combined sources5
Beta strandi95 – 98Combined sources4
Helixi99 – 109Combined sources11
Helixi116 – 118Combined sources3
Beta strandi119 – 121Combined sources3
Helixi125 – 133Combined sources9
Helixi135 – 153Combined sources19
Helixi160 – 163Combined sources4
Beta strandi167 – 170Combined sources4
Helixi171 – 188Combined sources18
Helixi195 – 197Combined sources3
Helixi198 – 220Combined sources23
Helixi225 – 227Combined sources3
Helixi229 – 241Combined sources13
Helixi244 – 247Combined sources4
Helixi255 – 267Combined sources13
Helixi272 – 287Combined sources16
Helixi290 – 303Combined sources14
Helixi305 – 321Combined sources17
Beta strandi328 – 331Combined sources4
Helixi337 – 341Combined sources5
Helixi344 – 357Combined sources14
Beta strandi358 – 360Combined sources3
Beta strandi362 – 373Combined sources12
Beta strandi378 – 381Combined sources4
Beta strandi386 – 389Combined sources4
Helixi392 – 395Combined sources4
Turni398 – 400Combined sources3
Beta strandi401 – 403Combined sources3
Turni409 – 412Combined sources4
Beta strandi417 – 419Combined sources3
Helixi447 – 464Combined sources18
Beta strandi465 – 469Combined sources5
Turni470 – 473Combined sources4
Helixi481 – 483Combined sources3
Beta strandi485 – 488Combined sources4
Beta strandi491 – 493Combined sources3
Beta strandi496 – 501Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DAXX-ray2.15A/B25-503[»]
3SN5X-ray2.75A/B25-503[»]
3V8DX-ray1.90A/B25-503[»]
ProteinModelPortaliP22680.
SMRiP22680.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22680.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00550000074551.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiP22680.
KOiK00489.
OMAiFRTAHNA.
OrthoDBiEOG091G07UI.
PhylomeDBiP22680.
TreeFamiTF105090.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR030681. Cholesterol_7a_monooxygenase.
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PANTHERiPTHR24304:SF1. PTHR24304:SF1. 1 hit.
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF500625. Cytochrome_CYP7A1. 1 hit.
PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMTTSLIWGI AIAACCCLWL ILGIRRRQTG EPPLENGLIP YLGCALQFGA
60 70 80 90 100
NPLEFLRANQ RKHGHVFTCK LMGKYVHFIT NPLSYHKVLC HGKYFDWKKF
110 120 130 140 150
HFATSAKAFG HRSIDPMDGN TTENINDTFI KTLQGHALNS LTESMMENLQ
160 170 180 190 200
RIMRPPVSSN SKTAAWVTEG MYSFCYRVMF EAGYLTIFGR DLTRRDTQKA
210 220 230 240 250
HILNNLDNFK QFDKVFPALV AGLPIHMFRT AHNAREKLAE SLRHENLQKR
260 270 280 290 300
ESISELISLR MFLNDTLSTF DDLEKAKTHL VVLWASQANT IPATFWSLFQ
310 320 330 340 350
MIRNPEAMKA ATEEVKRTLE NAGQKVSLEG NPICLSQAEL NDLPVLDSII
360 370 380 390 400
KESLRLSSAS LNIRTAKEDF TLHLEDGSYN IRKDDIIALY PQLMHLDPEI
410 420 430 440 450
YPDPLTFKYD RYLDENGKTK TTFYCNGLKL KYYYMPFGSG ATICPGRLFA
460 470 480 490 500
IHEIKQFLIL MLSYFELELI EGQAKCPPLD QSRAGLGILP PLNDIEFKYK

FKHL
Length:504
Mass (Da):57,661
Last modified:June 1, 1994 - v2
Checksum:iD8067E0FF6342949
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti385D → S in CAA39568 (PubMed:2384150).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05915286H → N.Corresponds to variant rs62621283dbSNPEnsembl.1
Natural variantiVAR_001259100F → S.1 Publication1
Natural variantiVAR_018376233N → S.1 PublicationCorresponds to variant rs8192874dbSNPEnsembl.1
Natural variantiVAR_018377347D → N.2 PublicationsCorresponds to variant rs8192875dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56088 mRNA. Translation: CAA39568.1.
M93133 mRNA. Translation: AAA58435.1.
BC101777 mRNA. Translation: AAI01778.1.
BC112184 mRNA. Translation: AAI12185.1.
L13460 Genomic DNA. Translation: AAA61350.1.
M89647 Genomic DNA. Translation: AAA58423.1.
CCDSiCCDS6171.1.
PIRiS29818. JH0659.
RefSeqiNP_000771.2. NM_000780.3.
UniGeneiHs.1644.

Genome annotation databases

EnsembliENST00000301645; ENSP00000301645; ENSG00000167910.
GeneIDi1581.
KEGGihsa:1581.
UCSCiuc003xtm.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

Cholesterol-7 alpha-hydroxylase entry

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56088 mRNA. Translation: CAA39568.1.
M93133 mRNA. Translation: AAA58435.1.
BC101777 mRNA. Translation: AAI01778.1.
BC112184 mRNA. Translation: AAI12185.1.
L13460 Genomic DNA. Translation: AAA61350.1.
M89647 Genomic DNA. Translation: AAA58423.1.
CCDSiCCDS6171.1.
PIRiS29818. JH0659.
RefSeqiNP_000771.2. NM_000780.3.
UniGeneiHs.1644.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DAXX-ray2.15A/B25-503[»]
3SN5X-ray2.75A/B25-503[»]
3V8DX-ray1.90A/B25-503[»]
ProteinModelPortaliP22680.
SMRiP22680.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000301645.

Chemistry databases

SwissLipidsiSLP:000001200.

PTM databases

iPTMnetiP22680.
PhosphoSitePlusiP22680.

Polymorphism and mutation databases

BioMutaiCYP7A1.
DMDMi544084.

Proteomic databases

EPDiP22680.
PaxDbiP22680.
PRIDEiP22680.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000301645; ENSP00000301645; ENSG00000167910.
GeneIDi1581.
KEGGihsa:1581.
UCSCiuc003xtm.5. human.

Organism-specific databases

CTDi1581.
DisGeNETi1581.
GeneCardsiCYP7A1.
HGNCiHGNC:2651. CYP7A1.
MalaCardsiCYP7A1.
MIMi118455. gene.
neXtProtiNX_P22680.
OpenTargetsiENSG00000167910.
Orphaneti209902. Hypercholesterolemia due to cholesterol 7alpha-hydroxylase deficiency.
PharmGKBiPA132.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00550000074551.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiP22680.
KOiK00489.
OMAiFRTAHNA.
OrthoDBiEOG091G07UI.
PhylomeDBiP22680.
TreeFamiTF105090.

Enzyme and pathway databases

UniPathwayiUPA00221.
BioCyciMetaCyc:HS09659-MONOMER.
ZFISH:HS09659-MONOMER.
BRENDAi1.14.13.17. 2681.
ReactomeiR-HSA-192105. Synthesis of bile acids and bile salts.
R-HSA-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-HSA-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
R-HSA-1989781. PPARA activates gene expression.
R-HSA-211976. Endogenous sterols.
SABIO-RKP22680.

Miscellaneous databases

EvolutionaryTraceiP22680.
GeneWikiiCholesterol_7_alpha-hydroxylase.
GenomeRNAii1581.
PROiP22680.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000167910.
CleanExiHS_CYP7A1.
GenevisibleiP22680. HS.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR030681. Cholesterol_7a_monooxygenase.
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PANTHERiPTHR24304:SF1. PTHR24304:SF1. 1 hit.
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF500625. Cytochrome_CYP7A1. 1 hit.
PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP7A1_HUMAN
AccessioniPrimary (citable) accession number: P22680
Secondary accession number(s): P78454, Q3MIL8, Q7KZ19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 165 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.