P22669 (GUN_ASPAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Endoglucanase-1 EC=3.2.1.4 Alternative name(s): Cellulase Endo-1,4-beta-glucanase Endoglucanase I FI-CMCase
Organism	Aspergillus aculeatus
Taxonomic identifier	5053 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted.
Induction	By cellulosic materials and hemicelluloses.
Miscellaneous	Will also hydrolyze 1,4-linkages in beta-D-glucans also containing 1,3-linkages.
Sequence similarities	Belongs to the glycosyl hydrolase 12 (cellulase H) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 237	221	Endoglucanase-1		PRO_0000008018
Amino acid modifications
	Modified residue	17	1	Pyrrolidone carboxylic acid Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P22669 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 8F173571A8AE6931	FASTA	237	25,560
	10 20 30 40 50 60 MKAFHLLAAL AGAAVAQQAQ LCDQYATYTG GVYTINNNLW GKDAGSGSQC TTVNSASSAG 70 80 90 100 110 120 TSWSTKWNWS GGENSVKSYA NSGLTFNKKL VSQISQIPTT ARWSYDNTGI RADVAYDLFT 130 140 150 160 170 180 AADINHVTWS GDYELMIWLA RYGGVQPIGS QIATATVDGQ TWELWYGANG SQKTYSFVAP 190 200 210 220 230 TPITSFQGDV NDFFKYLTQN HGFPASSQYL ITLQFGTEPF TGGPATLSVS NWSASVQ

References

[1]	"Complete nucleotide sequence of a gene coding for Aspergillus aculeatus cellulase (FI-CMCase)." Ooi T., Shinmyo A., Okada H., Murao S., Kawaguchi T., Arai M. Nucleic Acids Res. 18:5884-5884(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: F-50.
[2]	"Cloning and sequence analysis of a cDNA for cellulase (FI-CMCase) from Aspergillus aculeatus." Ooi T., Shinmyo A., Okada H., Hara S., Ikenaka T., Murao S., Arai M. Curr. Genet. 18:217-222(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: F-50.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D00546 Genomic DNA. Translation: BAA00435.1. X52525 mRNA. Translation: CAA36757.1.
PIR	S12610.
3D structure databases
ProteinModelPortal	P22669.
SMR	P22669. Positions 21-236.
ModBase	Search...
Protein family/group databases
CAZy	GH12. Glycoside Hydrolase Family 12.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.120.180. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013319. Glyco_hydro_11/12. IPR002594. Glyco_hydro_12. [Graphical view]
Pfam	PF01670. Glyco_hydro_12. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GUN_ASPAC

Accession

Primary (citable) accession number: P22669

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program