Reviewed, UniProtKB/Swiss-Prot P22661 (HOXH_RHOOP)

Names and origin

Protein names

Recommended name:
    NAD-reducing hydrogenase hoxS subunit beta
    EC=1.12.1.2

Gene names

Name:

hoxH

Encoded on

Plasmid

Organism

Rhodococcus opacus (Nocardia opaca)

Taxonomic identifier

37919 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	38 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	H2 + NAD+ = H+ + NADH.
Cofactor	FMN. Nickel.
Subunit structure	Tetramer of an alpha and a gamma subunits (flavin-containing dimer), and a delta and a nickel-containing beta subunits (hydrogenase dimer).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Nickel
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hydrogen dehydrogenase activity Inferred from electronic annotation. Source: EC nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›38	›38	NAD-reducing hydrogenase hoxS subunit beta		PRO_0000199720
Experimental info
	Non-terminal residue	38	1

Sequences

	Sequence		Length	Mass (Da)
	P22661-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 227487B5299C29C2	FASTA	38	4,219
	10 20 30 STKLVIDPVT RIEGHGKVTV HLDDNNNVVD AHLHVVEF

References

[1]

"Comparison of the NH2-terminal amino acid sequences of the four non-identical subunits of the NAD-linked hydrogenases from Nocardia opaca 1b and Alcaligenes eutrophus H16." Zaborosch C., Schneider K., Schlegel H.G., Kratzin H. Eur. J. Biochem. 181:175-180(1989) [PubMed: 2496982] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: 1B.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.12.1.2. 2206.
Family and domain databases
InterPro	IPR018194. Ni-dep_hyd_lsu_Ni_BS. [Graphical view]
PROSITE	PS00507. NI_HGENASE_L_1. Partial match. PS00508. NI_HGENASE_L_2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOXH_RHOOP

Accession

Primary (citable) accession number: P22661

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)