P22659 (HOXU_RHOOP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD-reducing hydrogenase hoxS subunit gamma
EC=1.12.1.2

Gene names

Name:

hoxU

Encoded on

Plasmid

Organism

Rhodococcus opacus (Nocardia opaca)

Taxonomic identifier

37919 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	33 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunits alpha and gamma of hoxS constitute an NADH--oxidoreductase.
Catalytic activity	H2 + NAD+ = H+ + NADH.
Cofactor	Binds 3 4Fe-4S clusters per subunit Potential.
Subunit structure	Tetramer of an alpha and a gamma subunits (flavin-containing dimer), and a delta and a nickel-containing beta subunits (hydrogenase dimer).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the complex I 75 kDa subunit family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	2Fe-2S 4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW hydrogen dehydrogenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›33	›33	NAD-reducing hydrogenase hoxS subunit gamma		PRO_0000118539
Regions
	Domain	1 – ›33	›33	2Fe-2S ferredoxin-type
Experimental info
	Non-terminal residue	33	1

Sequences

	Sequence		Length	Mass (Da)
	P22659 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 62AF6E43F64E719D	FASTA	33	3,492
	10 20 30 SIEIEIDGVT VTTEESRTLV DVAAEAGVYI PTL

References

[1]

"Comparison of the NH2-terminal amino acid sequences of the four non-identical subunits of the NAD-linked hydrogenases from Nocardia opaca 1b and Alcaligenes eutrophus H16." Zaborosch C., Schneider K., Schlegel H.G., Kratzin H. Eur. J. Biochem. 181:175-180(1989) [PubMed: 2496982] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: 1B.

Cross-references

3D structure databases
ProteinModelPortal	P22659.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR012675. Beta-grasp_ferredoxin-type. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. Partial match. PS51085. 2FE2S_FER_2. Partial match. PS00641. COMPLEX1_75K_1. Partial match. PS00642. COMPLEX1_75K_2. Partial match. PS00643. COMPLEX1_75K_3. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOXU_RHOOP

Accession

Primary (citable) accession number: P22659

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	June 28, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program