P22658 (HOXF_RHOOP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NAD-reducing hydrogenase hoxS subunit alpha
EC=1.12.1.2

Gene names

Name:

hoxF

Encoded on

Plasmid

Organism

Rhodococcus opacus (Nocardia opaca)

Taxonomic identifier

37919 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	37 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunits alpha and gamma of hoxS constitute an NADH--oxidoreductase.
Catalytic activity	H2 + NAD+ = H+ + NADH.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Subunit structure	Tetramer of an alpha and a gamma subunits (flavin-containing dimer), and a delta and a nickel-containing beta subunits (hydrogenase dimer).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW hydrogen dehydrogenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›37	›37	NAD-reducing hydrogenase hoxS subunit alpha		PRO_0000118565
Experimental info
	Non-terminal residue	37	1

Sequences

	Sequence		Length	Mass (Da)
	P22658 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 5A1C36E68B866C9D	FASTA	37	4,273
	10 20 30 SGDIKAILER NGSERTRLID ILWDVQHLYG HIPDEVL

References

[1]

"Comparison of the NH2-terminal amino acid sequences of the four non-identical subunits of the NAD-linked hydrogenases from Nocardia opaca 1b and Alcaligenes eutrophus H16." Zaborosch C., Schneider K., Schlegel H.G., Kratzin H. Eur. J. Biochem. 181:175-180(1989) [PubMed: 2496982] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: 1B.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
PROSITE	PS00644. COMPLEX1_51K_1. Partial match. PS00645. COMPLEX1_51K_2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOXF_RHOOP

Accession

Primary (citable) accession number: P22658

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	June 28, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program