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Protein

Thymidine kinase

Gene

TK

Organism
Bovine herpesvirus 2 (strain BHM-1) (BoHV-2) (Bovine mammillitis virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome.By similarity

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei36 – 361Proton acceptorSequence Analysis
Binding sitei54 – 541SubstrateBy similarity
Binding sitei78 – 781SubstrateBy similarity
Binding sitei125 – 1251SubstrateBy similarity
Binding sitei169 – 1691ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 188ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. thymidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA biosynthetic process Source: UniProtKB-KW
  2. TMP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
OrganismiBovine herpesvirus 2 (strain BHM-1) (BoHV-2) (Bovine mammillitis virus)
Taxonomic identifieri10297 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Thymidine kinasePRO_0000175063Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP22649.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P22649-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLLRVYVD GPHGLGKTTA ASALASERGD AIYLPEPMSY WSGAGEDDLV
60 70 80 90 100
ARVYTAQHRM DRGEIDAREA AGVVLGAQLT MSTPYVALNG LIAPHIGEEP
110 120 130 140 150
SPGNATPPDL ILIFDRHPIA SLLCYPLARY LTRCLPIESV LSLIALIPPT
160 170 180 190 200
PPGTNLILGT APAEDHLSRL VARGRPGELP DARMLRAIRY VYALLANTVK
210 220 230 240 250
YLQSGGSWRA DLGSEPPRLP LAPPEIGDPN NPGGHNTLLA LIHGAGATRG
260 270 280 290 300
CAAMTSWTLD LLADRLRSMN MFTVDYSAPP EACVAQMRNL LPSMQCTVTP
310 320
KPESHRAVAD AALAFMREMS NCK
Length:323
Mass (Da):34,661
Last modified:May 26, 2002 - v2
Checksum:i5AB03D3F17EF1199
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00537 Genomic DNA. Translation: BAA00425.2.
PIRiJT0532. KIBEB2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00537 Genomic DNA. Translation: BAA00425.2.
PIRiJT0532. KIBEB2.

3D structure databases

ProteinModelPortaliP22649.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Location and characterization of the bovine herpesvirus type 2 thymidine kinase gene."
    Sheppard M., May J.T.
    J. Gen. Virol. 70:3067-3071(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Sheppard M., May J.T.
    Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO C-TERMINUS.

Entry informationi

Entry nameiKITH_BHV2H
AccessioniPrimary (citable) accession number: P22649
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 31, 1991
Last sequence update: May 26, 2002
Last modified: January 6, 2015
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.