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P22646

- CD3E_MOUSE

UniProt

P22646 - CD3E_MOUSE

Protein

T-cell surface glycoprotein CD3 epsilon chain

Gene

Cd3e

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    The CD3 complex mediates signal transduction.

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. signal transducer activity Source: MGI
    3. transmembrane signaling receptor activity Source: InterPro

    GO - Biological processi

    1. apoptotic signaling pathway Source: MGI
    2. cell surface receptor signaling pathway Source: MGI
    3. lymphocyte activation Source: MGI
    4. negative regulation of smoothened signaling pathway Source: MGI
    5. negative thymic T cell selection Source: MGI
    6. positive regulation of alpha-beta T cell proliferation Source: MGI
    7. positive regulation of calcium-mediated signaling Source: MGI
    8. positive regulation of interferon-gamma production Source: MGI
    9. positive regulation of interleukin-2 biosynthetic process Source: MGI
    10. positive regulation of interleukin-4 production Source: MGI
    11. positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
    12. positive regulation of T cell activation Source: MGI
    13. positive regulation of T cell anergy Source: MGI
    14. positive regulation of T cell proliferation Source: MGI
    15. response to nutrient Source: Ensembl
    16. T cell costimulation Source: MGI
    17. T cell receptor signaling pathway Source: MGI

    Keywords - Molecular functioni

    Receptor

    Enzyme and pathway databases

    ReactomeiREACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
    REACT_215414. Translocation of ZAP-70 to Immunological synapse.
    REACT_216080. Phosphorylation of CD3 and TCR zeta chains.
    REACT_225145. Downstream TCR signaling.
    REACT_225768. Generation of second messenger molecules.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    T-cell surface glycoprotein CD3 epsilon chain
    Alternative name(s):
    T-cell surface antigen T3/Leu-4 epsilon chain
    CD_antigen: CD3e
    Gene namesi
    Name:Cd3e
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:88332. Cd3e.

    Subcellular locationi

    GO - Cellular componenti

    1. alpha-beta T cell receptor complex Source: MGI
    2. cell-cell junction Source: MGI
    3. external side of plasma membrane Source: MGI
    4. immunological synapse Source: MGI
    5. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Add
    BLAST
    Chaini22 – 189168T-cell surface glycoprotein CD3 epsilon chainPRO_0000014609Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi42 ↔ 83
    Modified residuei170 – 1701PhosphotyrosinePROSITE-ProRule annotation
    Modified residuei181 – 1811PhosphotyrosinePROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    Proteomic databases

    PaxDbiP22646.
    PRIDEiP22646.

    PTM databases

    PhosphoSiteiP22646.

    Expressioni

    Gene expression databases

    ArrayExpressiP22646.
    BgeeiP22646.
    CleanExiMM_CD3E.
    GenevestigatoriP22646.

    Interactioni

    Subunit structurei

    The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.

    Protein-protein interaction databases

    BioGridi198596. 6 interactions.
    IntActiP22646. 1 interaction.
    MINTiMINT-7895702.

    Structurei

    Secondary structure

    1
    189
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi30 – 345
    Beta strandi37 – 415
    Beta strandi51 – 544
    Beta strandi57 – 593
    Beta strandi65 – 717
    Turni74 – 763
    Beta strandi79 – 846
    Beta strandi94 – 963
    Turni135 – 1373
    Helixi144 – 1463
    Helixi168 – 1703
    Helixi179 – 1824
    Helixi183 – 1853
    Turni186 – 1883

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JBJNMR-A22-100[»]
    1XMWNMR-A22-100[»]
    2K4FNMR-A134-189[»]
    3R08X-ray4.10E22-100[»]
    ProteinModelPortaliP22646.
    SMRiP22646. Positions 22-100, 134-189.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP22646.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini23 – 10886ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini135 – 18955CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei109 – 13426HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 9977Ig-likeAdd
    BLAST
    Domaini160 – 18728ITAMPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ITAM domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG78872.
    GeneTreeiENSGT00510000048592.
    HOGENOMiHOG000290664.
    HOVERGENiHBG102121.
    InParanoidiP22646.
    KOiK06451.
    OMAiDIEWIST.
    OrthoDBiEOG7N0C6H.
    PhylomeDBiP22646.
    TreeFamiTF335892.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    InterProiIPR015484. CD3_esu/gsu/dsu.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003598. Ig_sub2.
    IPR003110. Phos_immunorcpt_sig_ITAM.
    [Graphical view]
    PANTHERiPTHR10570. PTHR10570. 1 hit.
    PfamiPF02189. ITAM. 1 hit.
    [Graphical view]
    SMARTiSM00408. IGc2. 1 hit.
    SM00077. ITAM. 1 hit.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 1 hit.
    PS51055. ITAM_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P22646-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRWNTFWGIL CLSLLAVGTC QDDAENIEYK VSISGTSVEL TCPLDSDENL    50
    KWEKNGQELP QKHDKHLVLQ DFSEVEDSGY YVCYTPASNK NTYLYLKARV 100
    CEYCVEVDLT AVAIIIIVDI CITLGLLMVI YYWSKNRKAK AKPVTRGTGA 150
    GSRPRGQNKE RPPPVPNPDY EPIRKGQRDL YSGLNQRAV 189
    Length:189
    Mass (Da):21,393
    Last modified:February 1, 1996 - v2
    Checksum:i5CE92F784FA13B96
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02990 mRNA. Translation: AAA40296.1.
    M23376
    , M23370, M23371, M23372, M23373, M23374, M23375 Genomic DNA. Translation: AAA72720.1.
    CCDSiCCDS23125.1.
    PIRiA31348.
    RefSeqiNP_031674.1. NM_007648.4.
    XP_006510029.1. XM_006509966.1.
    UniGeneiMm.210361.

    Genome annotation databases

    EnsembliENSMUST00000102832; ENSMUSP00000099896; ENSMUSG00000032093.
    GeneIDi12501.
    KEGGimmu:12501.
    UCSCiuc009pez.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02990 mRNA. Translation: AAA40296.1 .
    M23376
    , M23370 , M23371 , M23372 , M23373 , M23374 , M23375 Genomic DNA. Translation: AAA72720.1 .
    CCDSi CCDS23125.1.
    PIRi A31348.
    RefSeqi NP_031674.1. NM_007648.4.
    XP_006510029.1. XM_006509966.1.
    UniGenei Mm.210361.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JBJ NMR - A 22-100 [» ]
    1XMW NMR - A 22-100 [» ]
    2K4F NMR - A 134-189 [» ]
    3R08 X-ray 4.10 E 22-100 [» ]
    ProteinModelPortali P22646.
    SMRi P22646. Positions 22-100, 134-189.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198596. 6 interactions.
    IntActi P22646. 1 interaction.
    MINTi MINT-7895702.

    PTM databases

    PhosphoSitei P22646.

    Proteomic databases

    PaxDbi P22646.
    PRIDEi P22646.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000102832 ; ENSMUSP00000099896 ; ENSMUSG00000032093 .
    GeneIDi 12501.
    KEGGi mmu:12501.
    UCSCi uc009pez.1. mouse.

    Organism-specific databases

    CTDi 916.
    MGIi MGI:88332. Cd3e.

    Phylogenomic databases

    eggNOGi NOG78872.
    GeneTreei ENSGT00510000048592.
    HOGENOMi HOG000290664.
    HOVERGENi HBG102121.
    InParanoidi P22646.
    KOi K06451.
    OMAi DIEWIST.
    OrthoDBi EOG7N0C6H.
    PhylomeDBi P22646.
    TreeFami TF335892.

    Enzyme and pathway databases

    Reactomei REACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
    REACT_215414. Translocation of ZAP-70 to Immunological synapse.
    REACT_216080. Phosphorylation of CD3 and TCR zeta chains.
    REACT_225145. Downstream TCR signaling.
    REACT_225768. Generation of second messenger molecules.

    Miscellaneous databases

    EvolutionaryTracei P22646.
    NextBioi 281450.
    PROi P22646.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P22646.
    Bgeei P22646.
    CleanExi MM_CD3E.
    Genevestigatori P22646.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    InterProi IPR015484. CD3_esu/gsu/dsu.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003598. Ig_sub2.
    IPR003110. Phos_immunorcpt_sig_ITAM.
    [Graphical view ]
    PANTHERi PTHR10570. PTHR10570. 1 hit.
    Pfami PF02189. ITAM. 1 hit.
    [Graphical view ]
    SMARTi SM00408. IGc2. 1 hit.
    SM00077. ITAM. 1 hit.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 1 hit.
    PS51055. ITAM_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolutionary relationship between the T3 chains of the T-cell receptor complex and the immunoglobulin supergene family."
      Gold D.P., Clevers H., Alarcon B., Dunlap S., Novotny J., Williams A.F., Terhorst C.
      Proc. Natl. Acad. Sci. U.S.A. 84:7649-7653(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Mechanisms contributing to T cell receptor signaling and assembly revealed by the solution structure of an ectodomain fragment of the CD3 epsilon gamma heterodimer."
      Sun Z.J., Kim K.S., Wagner G., Reinherz E.L.
      Cell 105:913-923(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 22-100.

    Entry informationi

    Entry nameiCD3E_MOUSE
    AccessioniPrimary (citable) accession number: P22646
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3