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Protein

T-cell surface glycoprotein CD3 epsilon chain

Gene

Cd3e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell developement (PubMed:19956738, PubMed:24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region.By similarity4 Publications

GO - Molecular functioni

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • apoptotic signaling pathway Source: MGI
  • cell surface receptor signaling pathway Source: MGI
  • cerebellum development Source: ARUK-UCL
  • dendrite development Source: ARUK-UCL
  • lymphocyte activation Source: MGI
  • negative regulation of gene expression Source: MGI
  • negative regulation of smoothened signaling pathway Source: MGI
  • negative thymic T cell selection Source: MGI
  • positive regulation of alpha-beta T cell proliferation Source: MGI
  • positive regulation of calcium-mediated signaling Source: MGI
  • positive regulation of gene expression Source: MGI
  • positive regulation of interferon-gamma production Source: MGI
  • positive regulation of interleukin-2 biosynthetic process Source: MGI
  • positive regulation of interleukin-4 production Source: MGI
  • positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  • positive regulation of T cell activation Source: MGI
  • positive regulation of T cell anergy Source: MGI
  • positive regulation of T cell proliferation Source: MGI
  • protein homooligomerization Source: MGI
  • response to nutrient Source: Ensembl
  • T cell costimulation Source: MGI
  • T cell receptor signaling pathway Source: MGI

Keywordsi

Molecular functionReceptor
Biological processAdaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-202424. Downstream TCR signaling.
R-MMU-202427. Phosphorylation of CD3 and TCR zeta chains.
R-MMU-202430. Translocation of ZAP-70 to Immunological synapse.
R-MMU-202433. Generation of second messenger molecules.
R-MMU-389948. PD-1 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD3 epsilon chain
Alternative name(s):
T-cell surface antigen T3/Leu-4 epsilon chain
CD_antigen: CD3e
Gene namesi
Name:Cd3e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:88332. Cd3e.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 108ExtracellularSequence analysisAdd BLAST86
Transmembranei109 – 134HelicalSequence analysisAdd BLAST26
Topological domaini135 – 189CytoplasmicSequence analysisAdd BLAST55

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Absence of CD3E leads to the complete absence of mature T-cells. Thymocyte development is arrested at the early double-negative (DN) stage.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000001460922 – 189T-cell surface glycoprotein CD3 epsilon chainAdd BLAST168

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi42 ↔ 83Combined sources1 Publication
Modified residuei170PhosphotyrosinePROSITE-ProRule annotationBy similarity1
Modified residuei181PhosphotyrosinePROSITE-ProRule annotationBy similarity1

Post-translational modificationi

Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiP22646.
MaxQBiP22646.
PaxDbiP22646.
PRIDEiP22646.

PTM databases

iPTMnetiP22646.
PhosphoSitePlusiP22646.
SwissPalmiP22646.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032093.
CleanExiMM_CD3E.
ExpressionAtlasiP22646. baseline and differential.
GenevisibleiP22646. MM.

Interactioni

Subunit structurei

The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. Interacts with CD6. Interacts with NCK1 (PubMed:24470497).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198596. 6 interactors.
CORUMiP22646.
IntActiP22646. 3 interactors.
MINTiMINT-7895702.
STRINGi10090.ENSMUSP00000099896.

Structurei

Secondary structure

1189
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 34Combined sources5
Beta strandi37 – 41Combined sources5
Beta strandi51 – 54Combined sources4
Beta strandi57 – 59Combined sources3
Beta strandi65 – 71Combined sources7
Turni74 – 76Combined sources3
Beta strandi79 – 84Combined sources6
Beta strandi94 – 96Combined sources3
Turni135 – 137Combined sources3
Helixi144 – 146Combined sources3
Helixi168 – 170Combined sources3
Helixi179 – 182Combined sources4
Helixi183 – 185Combined sources3
Turni186 – 188Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JBJNMR-A22-100[»]
1XMWNMR-A22-100[»]
2K4FNMR-A134-189[»]
3R08X-ray4.10E22-100[»]
ProteinModelPortaliP22646.
SMRiP22646.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22646.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 99Ig-likeAdd BLAST77
Domaini160 – 187ITAMPROSITE-ProRule annotationAdd BLAST28

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYD1. Eukaryota.
ENOG41123JN. LUCA.
GeneTreeiENSGT00510000048592.
HOGENOMiHOG000290664.
HOVERGENiHBG102121.
InParanoidiP22646.
KOiK06451.
OMAiQRGQNKE.
OrthoDBiEOG091G0PKE.
PhylomeDBiP22646.
TreeFamiTF335892.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiView protein in InterPro
IPR015484. CD3_esu/gsu/dsu.
IPR007110. Ig-like_dom.
IPR036179. Ig-like_dom_sf.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR003110. Phos_immunorcpt_sig_ITAM.
PANTHERiPTHR10570. PTHR10570. 1 hit.
PfamiView protein in Pfam
PF02189. ITAM. 1 hit.
SMARTiView protein in SMART
SM00408. IGc2. 1 hit.
SM00077. ITAM. 1 hit.
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiView protein in PROSITE
PS50835. IG_LIKE. 1 hit.
PS51055. ITAM_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRWNTFWGIL CLSLLAVGTC QDDAENIEYK VSISGTSVEL TCPLDSDENL
60 70 80 90 100
KWEKNGQELP QKHDKHLVLQ DFSEVEDSGY YVCYTPASNK NTYLYLKARV
110 120 130 140 150
CEYCVEVDLT AVAIIIIVDI CITLGLLMVI YYWSKNRKAK AKPVTRGTGA
160 170 180
GSRPRGQNKE RPPPVPNPDY EPIRKGQRDL YSGLNQRAV
Length:189
Mass (Da):21,393
Last modified:February 1, 1996 - v2
Checksum:i5CE92F784FA13B96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02990 mRNA. Translation: AAA40296.1.
M23376
, M23370, M23371, M23372, M23373, M23374, M23375 Genomic DNA. Translation: AAA72720.1.
CCDSiCCDS23125.1.
PIRiA31348.
RefSeqiNP_031674.1. NM_007648.4.
XP_006510029.1. XM_006509966.3.
UniGeneiMm.210361.

Genome annotation databases

EnsembliENSMUST00000102832; ENSMUSP00000099896; ENSMUSG00000032093.
GeneIDi12501.
KEGGimmu:12501.
UCSCiuc009pez.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiCD3E_MOUSE
AccessioniPrimary (citable) accession number: P22646
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1996
Last modified: November 22, 2017
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references