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Protein

T-cell surface glycoprotein CD3 epsilon chain

Gene

Cd3e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The CD3 complex mediates signal transduction.

GO - Molecular functioni

GO - Biological processi

  • apoptotic signaling pathway Source: MGI
  • cell surface receptor signaling pathway Source: MGI
  • lymphocyte activation Source: MGI
  • negative regulation of smoothened signaling pathway Source: MGI
  • negative thymic T cell selection Source: MGI
  • positive regulation of alpha-beta T cell proliferation Source: MGI
  • positive regulation of calcium-mediated signaling Source: MGI
  • positive regulation of interferon-gamma production Source: MGI
  • positive regulation of interleukin-2 biosynthetic process Source: MGI
  • positive regulation of interleukin-4 production Source: MGI
  • positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  • positive regulation of T cell activation Source: MGI
  • positive regulation of T cell anergy Source: MGI
  • positive regulation of T cell proliferation Source: MGI
  • response to nutrient Source: Ensembl
  • T cell costimulation Source: MGI
  • T cell receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Enzyme and pathway databases

ReactomeiREACT_280649. Downstream TCR signaling.
REACT_300990. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
REACT_309115. PD-1 signaling.
REACT_334692. Generation of second messenger molecules.
REACT_345130. Translocation of ZAP-70 to Immunological synapse.
REACT_345471. Phosphorylation of CD3 and TCR zeta chains.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD3 epsilon chain
Alternative name(s):
T-cell surface antigen T3/Leu-4 epsilon chain
CD_antigen: CD3e
Gene namesi
Name:Cd3e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:88332. Cd3e.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 10886ExtracellularSequence AnalysisAdd
BLAST
Transmembranei109 – 13426HelicalSequence AnalysisAdd
BLAST
Topological domaini135 – 18955CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • alpha-beta T cell receptor complex Source: MGI
  • cell-cell junction Source: MGI
  • external side of plasma membrane Source: MGI
  • immunological synapse Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MGI
  • T cell receptor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Add
BLAST
Chaini22 – 189168T-cell surface glycoprotein CD3 epsilon chainPRO_0000014609Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 83
Modified residuei170 – 1701PhosphotyrosinePROSITE-ProRule annotation
Modified residuei181 – 1811PhosphotyrosinePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP22646.
PRIDEiP22646.

PTM databases

PhosphoSiteiP22646.

Expressioni

Gene expression databases

BgeeiP22646.
CleanExiMM_CD3E.
ExpressionAtlasiP22646. baseline and differential.
GenevisibleiP22646. MM.

Interactioni

Subunit structurei

The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.

Protein-protein interaction databases

BioGridi198596. 6 interactions.
IntActiP22646. 1 interaction.
MINTiMINT-7895702.
STRINGi10090.ENSMUSP00000099896.

Structurei

Secondary structure

1
189
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi30 – 345Combined sources
Beta strandi37 – 415Combined sources
Beta strandi51 – 544Combined sources
Beta strandi57 – 593Combined sources
Beta strandi65 – 717Combined sources
Turni74 – 763Combined sources
Beta strandi79 – 846Combined sources
Beta strandi94 – 963Combined sources
Turni135 – 1373Combined sources
Helixi144 – 1463Combined sources
Helixi168 – 1703Combined sources
Helixi179 – 1824Combined sources
Helixi183 – 1853Combined sources
Turni186 – 1883Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JBJNMR-A22-100[»]
1XMWNMR-A22-100[»]
2K4FNMR-A134-189[»]
3R08X-ray4.10E22-100[»]
ProteinModelPortaliP22646.
SMRiP22646. Positions 22-100, 134-189.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22646.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 9977Ig-likeAdd
BLAST
Domaini160 – 18728ITAMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ITAM domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG78872.
GeneTreeiENSGT00510000048592.
HOGENOMiHOG000290664.
HOVERGENiHBG102121.
InParanoidiP22646.
KOiK06451.
OMAiYLRARVC.
OrthoDBiEOG7N0C6H.
PhylomeDBiP22646.
TreeFamiTF335892.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR015484. CD3_esu/gsu/dsu.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR003110. Phos_immunorcpt_sig_ITAM.
[Graphical view]
PANTHERiPTHR10570. PTHR10570. 1 hit.
PfamiPF02189. ITAM. 1 hit.
[Graphical view]
SMARTiSM00408. IGc2. 1 hit.
SM00077. ITAM. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51055. ITAM_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRWNTFWGIL CLSLLAVGTC QDDAENIEYK VSISGTSVEL TCPLDSDENL
60 70 80 90 100
KWEKNGQELP QKHDKHLVLQ DFSEVEDSGY YVCYTPASNK NTYLYLKARV
110 120 130 140 150
CEYCVEVDLT AVAIIIIVDI CITLGLLMVI YYWSKNRKAK AKPVTRGTGA
160 170 180
GSRPRGQNKE RPPPVPNPDY EPIRKGQRDL YSGLNQRAV
Length:189
Mass (Da):21,393
Last modified:February 1, 1996 - v2
Checksum:i5CE92F784FA13B96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02990 mRNA. Translation: AAA40296.1.
M23376
, M23370, M23371, M23372, M23373, M23374, M23375 Genomic DNA. Translation: AAA72720.1.
CCDSiCCDS23125.1.
PIRiA31348.
RefSeqiNP_031674.1. NM_007648.4.
XP_006510029.1. XM_006509966.2.
UniGeneiMm.210361.

Genome annotation databases

EnsembliENSMUST00000102832; ENSMUSP00000099896; ENSMUSG00000032093.
GeneIDi12501.
KEGGimmu:12501.
UCSCiuc009pez.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02990 mRNA. Translation: AAA40296.1.
M23376
, M23370, M23371, M23372, M23373, M23374, M23375 Genomic DNA. Translation: AAA72720.1.
CCDSiCCDS23125.1.
PIRiA31348.
RefSeqiNP_031674.1. NM_007648.4.
XP_006510029.1. XM_006509966.2.
UniGeneiMm.210361.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JBJNMR-A22-100[»]
1XMWNMR-A22-100[»]
2K4FNMR-A134-189[»]
3R08X-ray4.10E22-100[»]
ProteinModelPortaliP22646.
SMRiP22646. Positions 22-100, 134-189.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198596. 6 interactions.
IntActiP22646. 1 interaction.
MINTiMINT-7895702.
STRINGi10090.ENSMUSP00000099896.

PTM databases

PhosphoSiteiP22646.

Proteomic databases

PaxDbiP22646.
PRIDEiP22646.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102832; ENSMUSP00000099896; ENSMUSG00000032093.
GeneIDi12501.
KEGGimmu:12501.
UCSCiuc009pez.1. mouse.

Organism-specific databases

CTDi916.
MGIiMGI:88332. Cd3e.

Phylogenomic databases

eggNOGiNOG78872.
GeneTreeiENSGT00510000048592.
HOGENOMiHOG000290664.
HOVERGENiHBG102121.
InParanoidiP22646.
KOiK06451.
OMAiYLRARVC.
OrthoDBiEOG7N0C6H.
PhylomeDBiP22646.
TreeFamiTF335892.

Enzyme and pathway databases

ReactomeiREACT_280649. Downstream TCR signaling.
REACT_300990. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
REACT_309115. PD-1 signaling.
REACT_334692. Generation of second messenger molecules.
REACT_345130. Translocation of ZAP-70 to Immunological synapse.
REACT_345471. Phosphorylation of CD3 and TCR zeta chains.

Miscellaneous databases

EvolutionaryTraceiP22646.
NextBioi281450.
PROiP22646.
SOURCEiSearch...

Gene expression databases

BgeeiP22646.
CleanExiMM_CD3E.
ExpressionAtlasiP22646. baseline and differential.
GenevisibleiP22646. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR015484. CD3_esu/gsu/dsu.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR003110. Phos_immunorcpt_sig_ITAM.
[Graphical view]
PANTHERiPTHR10570. PTHR10570. 1 hit.
PfamiPF02189. ITAM. 1 hit.
[Graphical view]
SMARTiSM00408. IGc2. 1 hit.
SM00077. ITAM. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51055. ITAM_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Evolutionary relationship between the T3 chains of the T-cell receptor complex and the immunoglobulin supergene family."
    Gold D.P., Clevers H., Alarcon B., Dunlap S., Novotny J., Williams A.F., Terhorst C.
    Proc. Natl. Acad. Sci. U.S.A. 84:7649-7653(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Mechanisms contributing to T cell receptor signaling and assembly revealed by the solution structure of an ectodomain fragment of the CD3 epsilon gamma heterodimer."
    Sun Z.J., Kim K.S., Wagner G., Reinherz E.L.
    Cell 105:913-923(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 22-100.

Entry informationi

Entry nameiCD3E_MOUSE
AccessioniPrimary (citable) accession number: P22646
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.