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P22646

- CD3E_MOUSE

UniProt

P22646 - CD3E_MOUSE

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Protein

T-cell surface glycoprotein CD3 epsilon chain

Gene

Cd3e

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The CD3 complex mediates signal transduction.

GO - Molecular functioni

  1. signal transducer activity Source: MGI
  2. transmembrane signaling receptor activity Source: InterPro

GO - Biological processi

  1. apoptotic signaling pathway Source: MGI
  2. cell surface receptor signaling pathway Source: MGI
  3. lymphocyte activation Source: MGI
  4. negative regulation of smoothened signaling pathway Source: MGI
  5. negative thymic T cell selection Source: MGI
  6. positive regulation of alpha-beta T cell proliferation Source: MGI
  7. positive regulation of calcium-mediated signaling Source: MGI
  8. positive regulation of interferon-gamma production Source: MGI
  9. positive regulation of interleukin-2 biosynthetic process Source: MGI
  10. positive regulation of interleukin-4 production Source: MGI
  11. positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  12. positive regulation of T cell activation Source: MGI
  13. positive regulation of T cell anergy Source: MGI
  14. positive regulation of T cell proliferation Source: MGI
  15. response to nutrient Source: Ensembl
  16. T cell costimulation Source: MGI
  17. T cell receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Enzyme and pathway databases

ReactomeiREACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
REACT_215414. Translocation of ZAP-70 to Immunological synapse.
REACT_216080. Phosphorylation of CD3 and TCR zeta chains.
REACT_225145. Downstream TCR signaling.
REACT_225768. Generation of second messenger molecules.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD3 epsilon chain
Alternative name(s):
T-cell surface antigen T3/Leu-4 epsilon chain
CD_antigen: CD3e
Gene namesi
Name:Cd3e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:88332. Cd3e.

Subcellular locationi

GO - Cellular componenti

  1. alpha-beta T cell receptor complex Source: MGI
  2. cell-cell junction Source: MGI
  3. external side of plasma membrane Source: MGI
  4. immunological synapse Source: MGI
  5. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Add
BLAST
Chaini22 – 189168T-cell surface glycoprotein CD3 epsilon chainPRO_0000014609Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 83
Modified residuei170 – 1701PhosphotyrosinePROSITE-ProRule annotation
Modified residuei181 – 1811PhosphotyrosinePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP22646.
PRIDEiP22646.

PTM databases

PhosphoSiteiP22646.

Expressioni

Gene expression databases

BgeeiP22646.
CleanExiMM_CD3E.
ExpressionAtlasiP22646. baseline and differential.
GenevestigatoriP22646.

Interactioni

Subunit structurei

The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.

Protein-protein interaction databases

BioGridi198596. 6 interactions.
IntActiP22646. 1 interaction.
MINTiMINT-7895702.

Structurei

Secondary structure

1
189
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi30 – 345
Beta strandi37 – 415
Beta strandi51 – 544
Beta strandi57 – 593
Beta strandi65 – 717
Turni74 – 763
Beta strandi79 – 846
Beta strandi94 – 963
Turni135 – 1373
Helixi144 – 1463
Helixi168 – 1703
Helixi179 – 1824
Helixi183 – 1853
Turni186 – 1883

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JBJNMR-A22-100[»]
1XMWNMR-A22-100[»]
2K4FNMR-A134-189[»]
3R08X-ray4.10E22-100[»]
ProteinModelPortaliP22646.
SMRiP22646. Positions 22-100, 134-189.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22646.

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 10886ExtracellularSequence AnalysisAdd
BLAST
Topological domaini135 – 18955CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei109 – 13426HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 9977Ig-likeAdd
BLAST
Domaini160 – 18728ITAMPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ITAM domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG78872.
GeneTreeiENSGT00510000048592.
HOGENOMiHOG000290664.
HOVERGENiHBG102121.
InParanoidiP22646.
KOiK06451.
OMAiDIEWIST.
OrthoDBiEOG7N0C6H.
PhylomeDBiP22646.
TreeFamiTF335892.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR015484. CD3_esu/gsu/dsu.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR003110. Phos_immunorcpt_sig_ITAM.
[Graphical view]
PANTHERiPTHR10570. PTHR10570. 1 hit.
PfamiPF02189. ITAM. 1 hit.
[Graphical view]
SMARTiSM00408. IGc2. 1 hit.
SM00077. ITAM. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51055. ITAM_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22646-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRWNTFWGIL CLSLLAVGTC QDDAENIEYK VSISGTSVEL TCPLDSDENL
60 70 80 90 100
KWEKNGQELP QKHDKHLVLQ DFSEVEDSGY YVCYTPASNK NTYLYLKARV
110 120 130 140 150
CEYCVEVDLT AVAIIIIVDI CITLGLLMVI YYWSKNRKAK AKPVTRGTGA
160 170 180
GSRPRGQNKE RPPPVPNPDY EPIRKGQRDL YSGLNQRAV
Length:189
Mass (Da):21,393
Last modified:February 1, 1996 - v2
Checksum:i5CE92F784FA13B96
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02990 mRNA. Translation: AAA40296.1.
M23376
, M23370, M23371, M23372, M23373, M23374, M23375 Genomic DNA. Translation: AAA72720.1.
CCDSiCCDS23125.1.
PIRiA31348.
RefSeqiNP_031674.1. NM_007648.4.
XP_006510029.1. XM_006509966.1.
UniGeneiMm.210361.

Genome annotation databases

EnsembliENSMUST00000102832; ENSMUSP00000099896; ENSMUSG00000032093.
GeneIDi12501.
KEGGimmu:12501.
UCSCiuc009pez.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02990 mRNA. Translation: AAA40296.1 .
M23376
, M23370 , M23371 , M23372 , M23373 , M23374 , M23375 Genomic DNA. Translation: AAA72720.1 .
CCDSi CCDS23125.1.
PIRi A31348.
RefSeqi NP_031674.1. NM_007648.4.
XP_006510029.1. XM_006509966.1.
UniGenei Mm.210361.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1JBJ NMR - A 22-100 [» ]
1XMW NMR - A 22-100 [» ]
2K4F NMR - A 134-189 [» ]
3R08 X-ray 4.10 E 22-100 [» ]
ProteinModelPortali P22646.
SMRi P22646. Positions 22-100, 134-189.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198596. 6 interactions.
IntActi P22646. 1 interaction.
MINTi MINT-7895702.

PTM databases

PhosphoSitei P22646.

Proteomic databases

PaxDbi P22646.
PRIDEi P22646.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000102832 ; ENSMUSP00000099896 ; ENSMUSG00000032093 .
GeneIDi 12501.
KEGGi mmu:12501.
UCSCi uc009pez.1. mouse.

Organism-specific databases

CTDi 916.
MGIi MGI:88332. Cd3e.

Phylogenomic databases

eggNOGi NOG78872.
GeneTreei ENSGT00510000048592.
HOGENOMi HOG000290664.
HOVERGENi HBG102121.
InParanoidi P22646.
KOi K06451.
OMAi DIEWIST.
OrthoDBi EOG7N0C6H.
PhylomeDBi P22646.
TreeFami TF335892.

Enzyme and pathway databases

Reactomei REACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
REACT_215414. Translocation of ZAP-70 to Immunological synapse.
REACT_216080. Phosphorylation of CD3 and TCR zeta chains.
REACT_225145. Downstream TCR signaling.
REACT_225768. Generation of second messenger molecules.

Miscellaneous databases

EvolutionaryTracei P22646.
NextBioi 281450.
PROi P22646.
SOURCEi Search...

Gene expression databases

Bgeei P22646.
CleanExi MM_CD3E.
ExpressionAtlasi P22646. baseline and differential.
Genevestigatori P22646.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
InterProi IPR015484. CD3_esu/gsu/dsu.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR003110. Phos_immunorcpt_sig_ITAM.
[Graphical view ]
PANTHERi PTHR10570. PTHR10570. 1 hit.
Pfami PF02189. ITAM. 1 hit.
[Graphical view ]
SMARTi SM00408. IGc2. 1 hit.
SM00077. ITAM. 1 hit.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 1 hit.
PS51055. ITAM_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Evolutionary relationship between the T3 chains of the T-cell receptor complex and the immunoglobulin supergene family."
    Gold D.P., Clevers H., Alarcon B., Dunlap S., Novotny J., Williams A.F., Terhorst C.
    Proc. Natl. Acad. Sci. U.S.A. 84:7649-7653(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Mechanisms contributing to T cell receptor signaling and assembly revealed by the solution structure of an ectodomain fragment of the CD3 epsilon gamma heterodimer."
    Sun Z.J., Kim K.S., Wagner G., Reinherz E.L.
    Cell 105:913-923(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 22-100.

Entry informationi

Entry nameiCD3E_MOUSE
AccessioniPrimary (citable) accession number: P22646
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3