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P22646 (CD3E_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
T-cell surface glycoprotein CD3 epsilon chain
Alternative name(s):
T-cell surface antigen T3/Leu-4 epsilon chain
CD_antigen=CD3e
Gene names
Name:Cd3e
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length189 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The CD3 complex mediates signal transduction.

Subunit structure

The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.

Subcellular location

Membrane; Single-pass type I membrane protein.

Sequence similarities

Contains 1 Ig-like (immunoglobulin-like) domain.

Contains 1 ITAM domain.

Ontologies

Keywords
   Cellular componentMembrane
   DomainImmunoglobulin domain
Signal
Transmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMDisulfide bond
Phosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processT cell receptor signaling pathway

Inferred from direct assay PubMed 10485657. Source: MGI

apoptotic signaling pathway

Inferred from direct assay PubMed 8666928. Source: MGI

cell surface receptor signaling pathway

Inferred from direct assay PubMed 7630421PubMed 8943715. Source: MGI

lymphocyte activation

Inferred from mutant phenotype PubMed 15843560. Source: MGI

negative regulation of smoothened signaling pathway

Inferred from direct assay PubMed 10981962. Source: MGI

negative thymic T cell selection

Inferred from direct assay PubMed 7630421. Source: MGI

positive regulation of T cell activation

Inferred from direct assay PubMed 12874832. Source: MGI

positive regulation of T cell anergy

Inferred from direct assay PubMed 14973438. Source: MGI

positive regulation of T cell proliferation

Inferred from direct assay PubMed 10072077PubMed 10485657PubMed 12874832PubMed 15368291. Source: MGI

positive regulation of alpha-beta T cell proliferation

Inferred from direct assay PubMed 11894097PubMed 14973438PubMed 8125140. Source: MGI

positive regulation of calcium-mediated signaling

Inferred from direct assay PubMed 7630421PubMed 8943715. Source: MGI

positive regulation of interferon-gamma production

Inferred from direct assay PubMed 11894097. Source: MGI

positive regulation of interleukin-2 biosynthetic process

Inferred from direct assay PubMed 12874832. Source: MGI

positive regulation of interleukin-4 production

Inferred from direct assay PubMed 11894097. Source: MGI

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from direct assay PubMed 10485657. Source: MGI

response to nutrient

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentalpha-beta T cell receptor complex

Inferred from direct assay PubMed 9064344PubMed 9208839. Source: MGI

external side of plasma membrane

Inferred from direct assay PubMed 10072077PubMed 10485657PubMed 15368291PubMed 17277142PubMed 19838199PubMed 20660734PubMed 20709950PubMed 22732588PubMed 7630421PubMed 8125140PubMed 8666928PubMed 8755570. Source: MGI

immunological synapse

Inferred from direct assay PubMed 14635057PubMed 15064761. Source: MGI

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionsignal transducer activity

Inferred from direct assay PubMed 7630421. Source: MGI

transmembrane signaling receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121
Chain22 – 189168T-cell surface glycoprotein CD3 epsilon chain
PRO_0000014609

Regions

Topological domain23 – 10886Extracellular Potential
Transmembrane109 – 13426Helical; Potential
Topological domain135 – 18955Cytoplasmic Potential
Domain23 – 9977Ig-like
Domain160 – 18728ITAM

Amino acid modifications

Modified residue1701Phosphotyrosine By similarity
Modified residue1811Phosphotyrosine By similarity
Disulfide bond42 ↔ 83

Secondary structure

........................... 189
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P22646 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 5CE92F784FA13B96

FASTA18921,393
        10         20         30         40         50         60 
MRWNTFWGIL CLSLLAVGTC QDDAENIEYK VSISGTSVEL TCPLDSDENL KWEKNGQELP 

        70         80         90        100        110        120 
QKHDKHLVLQ DFSEVEDSGY YVCYTPASNK NTYLYLKARV CEYCVEVDLT AVAIIIIVDI 

       130        140        150        160        170        180 
CITLGLLMVI YYWSKNRKAK AKPVTRGTGA GSRPRGQNKE RPPPVPNPDY EPIRKGQRDL 


YSGLNQRAV 

« Hide

References

[1]"Evolutionary relationship between the T3 chains of the T-cell receptor complex and the immunoglobulin supergene family."
Gold D.P., Clevers H., Alarcon B., Dunlap S., Novotny J., Williams A.F., Terhorst C.
Proc. Natl. Acad. Sci. U.S.A. 84:7649-7653(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Characterization and expression of the murine CD3-epsilon gene."
Clevers H., Dunlap S., Saito H., Georgopoulos K., Wileman T., Terhorst C.
Proc. Natl. Acad. Sci. U.S.A. 85:8623-8627(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Mechanisms contributing to T cell receptor signaling and assembly revealed by the solution structure of an ectodomain fragment of the CD3 epsilon gamma heterodimer."
Sun Z.J., Kim K.S., Wagner G., Reinherz E.L.
Cell 105:913-923(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 22-100.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02990 mRNA. Translation: AAA40296.1.
M23376 expand/collapse EMBL AC list , M23370, M23371, M23372, M23373, M23374, M23375 Genomic DNA. Translation: AAA72720.1.
PIRA31348.
RefSeqNP_031674.1. NM_007648.4.
XP_006510029.1. XM_006509966.1.
UniGeneMm.210361.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JBJNMR-A22-100[»]
1XMWNMR-A22-100[»]
2K4FNMR-A134-189[»]
3R08X-ray4.10E22-100[»]
ProteinModelPortalP22646.
SMRP22646. Positions 22-100, 134-189.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198596. 6 interactions.
IntActP22646. 1 interaction.
MINTMINT-7895702.

PTM databases

PhosphoSiteP22646.

Proteomic databases

PaxDbP22646.
PRIDEP22646.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102832; ENSMUSP00000099896; ENSMUSG00000032093.
GeneID12501.
KEGGmmu:12501.
UCSCuc009pez.1. mouse.

Organism-specific databases

CTD916.
MGIMGI:88332. Cd3e.

Phylogenomic databases

eggNOGNOG78872.
GeneTreeENSGT00510000048592.
HOGENOMHOG000290664.
HOVERGENHBG102121.
InParanoidP22646.
KOK06451.
OMAIVDICIT.
OrthoDBEOG7N0C6H.
PhylomeDBP22646.
TreeFamTF335892.

Gene expression databases

ArrayExpressP22646.
BgeeP22646.
CleanExMM_CD3E.
GenevestigatorP22646.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR015477. CD3_epsilon.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR003110. Phos_immunorcpt_sig_ITAM.
[Graphical view]
PANTHERPTHR10506. PTHR10506. 1 hit.
PfamPF02189. ITAM. 1 hit.
[Graphical view]
SMARTSM00408. IGc2. 1 hit.
SM00077. ITAM. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
PS51055. ITAM_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP22646.
NextBio281450.
PROP22646.
SOURCESearch...

Entry information

Entry nameCD3E_MOUSE
AccessionPrimary (citable) accession number: P22646
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot