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P22643

- DHLA_XANAU

UniProt

P22643 - DHLA_XANAU

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Protein

Haloalkane dehalogenase

Gene

dhlA

Organism
Xanthobacter autotrophicus
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes terminally mono- and di- chlorinated and brominated alkanes (up to C4 only). The highest activity was found with 1,2-dichloroethane, 1,3-dichloropropane, and 1,2-dibromoethane.

Catalytic activityi

1-haloalkane + H2O = a primary alcohol + halide.
1,2-dichloroethane + H2O = 2-chloroethanol + hydrogen chloride.

Enzyme regulationi

Inhibited by thiol reagents such as p-chloromercuribenzoate and iodoacetamide.

pH dependencei

Optimum pH is 8.2.

Temperature dependencei

Optimum temperature is 37 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei124 – 1241Nucleophile
Binding sitei125 – 1251Halide
Binding sitei175 – 1751Halide
Active sitei260 – 2601Proton donor
Active sitei289 – 2891Proton acceptor

GO - Molecular functioni

  1. haloalkane dehalogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 1,2-dichloroethane catabolic process Source: UniProtKB-UniPathway
  2. response to toxic substance Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Detoxification

Enzyme and pathway databases

UniPathwayiUPA00265; UER00387.

Protein family/group databases

MEROPSiS33.990.

Names & Taxonomyi

Protein namesi
Recommended name:
Haloalkane dehalogenase (EC:3.8.1.5)
Gene namesi
Name:dhlA
OrganismiXanthobacter autotrophicus
Taxonomic identifieri280 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Haloalkane dehalogenasePRO_0000216771Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
310
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 124Combined sources
Beta strandi23 – 275Combined sources
Beta strandi35 – 428Combined sources
Beta strandi49 – 524Combined sources
Helixi60 – 634Combined sources
Turni64 – 663Combined sources
Helixi67 – 726Combined sources
Beta strandi76 – 805Combined sources
Beta strandi90 – 923Combined sources
Helixi94 – 963Combined sources
Helixi99 – 11315Combined sources
Beta strandi117 – 1226Combined sources
Helixi125 – 1306Combined sources
Helixi134 – 1363Combined sources
Helixi138 – 1403Combined sources
Beta strandi141 – 1488Combined sources
Turni155 – 1573Combined sources
Helixi160 – 1634Combined sources
Turni164 – 1663Combined sources
Turni169 – 1713Combined sources
Helixi172 – 1809Combined sources
Helixi187 – 1948Combined sources
Helixi200 – 2078Combined sources
Helixi213 – 2153Combined sources
Helixi217 – 22711Combined sources
Helixi231 – 24616Combined sources
Beta strandi250 – 2578Combined sources
Beta strandi261 – 2644Combined sources
Helixi265 – 27410Combined sources
Beta strandi282 – 2843Combined sources
Helixi291 – 2944Combined sources
Helixi295 – 30814Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B6GX-ray1.15A3-310[»]
1BE0X-ray1.96A3-310[»]
1BEEX-ray2.60A3-310[»]
1BEZX-ray2.10A3-310[»]
1CIJX-ray2.30A3-310[»]
1EDBX-ray2.01A1-310[»]
1EDDX-ray2.19A1-310[»]
1EDEX-ray1.90A1-310[»]
1HDEX-ray2.70A/B1-310[»]
2DHCX-ray2.30A1-310[»]
2DHDX-ray2.13A1-310[»]
2DHEX-ray2.13A1-310[»]
2EDAX-ray2.19A1-310[»]
2EDCX-ray2.30A1-310[»]
2HADX-ray1.90A1-310[»]
2PKYX-ray1.55X1-310[»]
2YXPX-ray1.53X1-310[»]
ProteinModelPortaliP22643.
SMRiP22643. Positions 1-310.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22643.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
HAMAPiMF_01230. Haloalk_dehal_type1.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR023489. Haloalkane_dehalogenase_1.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

P22643-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MINAIRTPDQ RFSNLDQYPF SPNYLDDLPG YPGLRAHYLD EGNSDAEDVF
60 70 80 90 100
LCLHGEPTWS YLYRKMIPVF AESGARVIAP DFFGFGKSDK PVDEEDYTFE
110 120 130 140 150
FHRNFLLALI ERLDLRNITL VVQDWGGFLG LTLPMADPSR FKRLIIMNAC
160 170 180 190 200
LMTDPVTQPA FSAFVTQPAD GFTAWKYDLV TPSDLRLDQF MKRWAPTLTE
210 220 230 240 250
AEASAYAAPF PDTSYQAGVR KFPKMVAQRD QACIDISTEA ISFWQNDWNG
260 270 280 290 300
QTFMAIGMKD KLLGPDVMYP MKALINGCPE PLEIADAGHF VQEFGEQVAR
310
EALKHFAETE
Length:310
Mass (Da):35,144
Last modified:February 1, 1995 - v2
Checksum:iFB32C94BE5D8940C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26950 Genomic DNA. Translation: AAA88691.1.
PIRiB43718.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26950 Genomic DNA. Translation: AAA88691.1 .
PIRi B43718.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1B6G X-ray 1.15 A 3-310 [» ]
1BE0 X-ray 1.96 A 3-310 [» ]
1BEE X-ray 2.60 A 3-310 [» ]
1BEZ X-ray 2.10 A 3-310 [» ]
1CIJ X-ray 2.30 A 3-310 [» ]
1EDB X-ray 2.01 A 1-310 [» ]
1EDD X-ray 2.19 A 1-310 [» ]
1EDE X-ray 1.90 A 1-310 [» ]
1HDE X-ray 2.70 A/B 1-310 [» ]
2DHC X-ray 2.30 A 1-310 [» ]
2DHD X-ray 2.13 A 1-310 [» ]
2DHE X-ray 2.13 A 1-310 [» ]
2EDA X-ray 2.19 A 1-310 [» ]
2EDC X-ray 2.30 A 1-310 [» ]
2HAD X-ray 1.90 A 1-310 [» ]
2PKY X-ray 1.55 X 1-310 [» ]
2YXP X-ray 1.53 X 1-310 [» ]
ProteinModelPortali P22643.
SMRi P22643. Positions 1-310.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi S33.990.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00265 ; UER00387 .

Miscellaneous databases

EvolutionaryTracei P22643.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
HAMAPi MF_01230. Haloalk_dehal_type1.
InterProi IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR023489. Haloalkane_dehalogenase_1.
[Graphical view ]
Pfami PF00561. Abhydrolase_1. 1 hit.
[Graphical view ]
PRINTSi PR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMi SSF53474. SSF53474. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning of 1,2-dichloroethane degradation genes of Xanthobacter autotrophicus GJ10 and expression and sequencing of the dhlA gene."
    Janssen D.B., Pries F., van der Ploeg J., Kazemier B., Terpstra P., Witholt B.
    J. Bacteriol. 171:6791-6799(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: GJ10.
  2. "Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10."
    Keuning S., Janssen D.B., Witholt B.
    J. Bacteriol. 163:635-639(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-10.
  3. "Crystallization of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10."
    Rozeboom H.J., Kingma J., Janssen D.B., Dijkstra B.W.
    J. Mol. Biol. 200:611-612(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
    Strain: GJ10.
  4. "Crystal structure of haloalkane dehalogenase: an enzyme to detoxify halogenated alkanes."
    Franken S.M., Rozeboom H.J., Kalk K.H., Dijkstra B.W.
    EMBO J. 10:1297-1302(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS), SEQUENCE REVISION TO 120.
    Strain: GJ10.
  5. "Refined X-ray structures of haloalkane dehalogenase at pH 6.2 and pH 8.2 and implications for the reaction mechanism."
    Verschueren K.H.G., Franken S.M., Rozeboom H.J., Kalk K.H., Dijkstra B.W.
    J. Mol. Biol. 232:856-872(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
  6. "Crystallographic analysis of the catalytic mechanism of haloalkane dehalogenase."
    Verschueren K.H.G., Seljee F., Rozeboom H.J., Kalk K.H., Dijkstra B.W.
    Nature 363:693-698(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
    Strain: GJ10.
  7. "Kinetic analysis and X-ray structure of haloalkane dehalogenase with a modified halide-binding site."
    Krooshof G.H., Ridder I.S., Tepper A.W.J.W., Vos G.J., Rozeboom H.J., Kalk K.H., Dijkstra B.W., Janssen D.B.
    Biochemistry 37:15013-15023(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS).
  8. "Crystallographic and kinetic evidence of a collision complex formed during halide import in haloalkane dehalogenase."
    Pikkemaat M.G., Ridder I.S., Rozeboom H.J., Kalk K.H., Dijkstra B.W., Janssen D.B.
    Biochemistry 38:12052-12061(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
  9. "Haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 refined at 1.15 A resolution."
    Ridder I.S., Rozeboom H.J., Dijkstra B.W.
    Acta Crystallogr. D 55:1273-1290(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.15 ANGSTROMS).
    Strain: GJ10.

Entry informationi

Entry nameiDHLA_XANAU
AccessioniPrimary (citable) accession number: P22643
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1995
Last modified: November 26, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3