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P22643 (DHLA_XANAU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Haloalkane dehalogenase

EC=3.8.1.5
Gene names
Name:dhlA
OrganismXanthobacter autotrophicus
Taxonomic identifier280 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter

Protein attributes

Sequence length310 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes terminally mono- and di- chlorinated and brominated alkanes (up to C4 only). The highest activity was found with 1,2-dichloroethane, 1,3-dichloropropane, and 1,2-dibromoethane. HAMAP-Rule MF_01230

Catalytic activity

1-haloalkane + H2O = a primary alcohol + halide. HAMAP-Rule MF_01230

1,2-dichloroethane + H2O = 2-chloroethanol + hydrogen chloride. HAMAP-Rule MF_01230

Enzyme regulation

Inhibited by thiol reagents such as p-chloromercuribenzoate and iodoacetamide. HAMAP-Rule MF_01230

Pathway

Xenobiotic degradation; 1,2-dichloroethane degradation; glycolate from 1,2-dichloroethane: step 1/4. HAMAP-Rule MF_01230

Subunit structure

Monomer.

Sequence similarities

Belongs to the haloalkane dehalogenase family. Type 1 subfamily.

Biophysicochemical properties

pH dependence:

Optimum pH is 8.2. HAMAP-Rule MF_01230

Temperature dependence:

Optimum temperature is 37 degrees Celsius.

Ontologies

Keywords
   Biological processDetoxification
   Molecular functionHydrolase
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_process1,2-dichloroethane catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

response to toxic substance

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionhaloalkane dehalogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 310310Haloalkane dehalogenase HAMAP-Rule MF_01230
PRO_0000216771

Sites

Active site1241Nucleophile
Active site2601Proton donor
Active site2891Proton acceptor
Binding site1251Halide
Binding site1751Halide

Secondary structure

.......................................................... 310
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P22643 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: FB32C94BE5D8940C

FASTA31035,144
        10         20         30         40         50         60 
MINAIRTPDQ RFSNLDQYPF SPNYLDDLPG YPGLRAHYLD EGNSDAEDVF LCLHGEPTWS 

        70         80         90        100        110        120 
YLYRKMIPVF AESGARVIAP DFFGFGKSDK PVDEEDYTFE FHRNFLLALI ERLDLRNITL 

       130        140        150        160        170        180 
VVQDWGGFLG LTLPMADPSR FKRLIIMNAC LMTDPVTQPA FSAFVTQPAD GFTAWKYDLV 

       190        200        210        220        230        240 
TPSDLRLDQF MKRWAPTLTE AEASAYAAPF PDTSYQAGVR KFPKMVAQRD QACIDISTEA 

       250        260        270        280        290        300 
ISFWQNDWNG QTFMAIGMKD KLLGPDVMYP MKALINGCPE PLEIADAGHF VQEFGEQVAR 

       310 
EALKHFAETE 

« Hide

References

[1]"Cloning of 1,2-dichloroethane degradation genes of Xanthobacter autotrophicus GJ10 and expression and sequencing of the dhlA gene."
Janssen D.B., Pries F., van der Ploeg J., Kazemier B., Terpstra P., Witholt B.
J. Bacteriol. 171:6791-6799(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: GJ10.
[2]"Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10."
Keuning S., Janssen D.B., Witholt B.
J. Bacteriol. 163:635-639(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-10.
[3]"Crystallization of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10."
Rozeboom H.J., Kingma J., Janssen D.B., Dijkstra B.W.
J. Mol. Biol. 200:611-612(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: CRYSTALLIZATION.
Strain: GJ10.
[4]"Crystal structure of haloalkane dehalogenase: an enzyme to detoxify halogenated alkanes."
Franken S.M., Rozeboom H.J., Kalk K.H., Dijkstra B.W.
EMBO J. 10:1297-1302(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS), SEQUENCE REVISION TO 120.
Strain: GJ10.
[5]"Refined X-ray structures of haloalkane dehalogenase at pH 6.2 and pH 8.2 and implications for the reaction mechanism."
Verschueren K.H.G., Franken S.M., Rozeboom H.J., Kalk K.H., Dijkstra B.W.
J. Mol. Biol. 232:856-872(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
[6]"Crystallographic analysis of the catalytic mechanism of haloalkane dehalogenase."
Verschueren K.H.G., Seljee F., Rozeboom H.J., Kalk K.H., Dijkstra B.W.
Nature 363:693-698(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
Strain: GJ10.
[7]"Kinetic analysis and X-ray structure of haloalkane dehalogenase with a modified halide-binding site."
Krooshof G.H., Ridder I.S., Tepper A.W.J.W., Vos G.J., Rozeboom H.J., Kalk K.H., Dijkstra B.W., Janssen D.B.
Biochemistry 37:15013-15023(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS).
[8]"Crystallographic and kinetic evidence of a collision complex formed during halide import in haloalkane dehalogenase."
Pikkemaat M.G., Ridder I.S., Rozeboom H.J., Kalk K.H., Dijkstra B.W., Janssen D.B.
Biochemistry 38:12052-12061(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[9]"Haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 refined at 1.15 A resolution."
Ridder I.S., Rozeboom H.J., Dijkstra B.W.
Acta Crystallogr. D 55:1273-1290(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.15 ANGSTROMS).
Strain: GJ10.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M26950 Genomic DNA. Translation: AAA88691.1.
PIRB43718.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B6GX-ray1.15A3-310[»]
1BE0X-ray1.96A3-310[»]
1BEEX-ray2.60A3-310[»]
1BEZX-ray2.10A3-310[»]
1CIJX-ray2.30A3-310[»]
1EDBX-ray2.01A1-310[»]
1EDDX-ray2.19A1-310[»]
1EDEX-ray1.90A1-310[»]
1HDEX-ray2.70A/B1-310[»]
2DHCX-ray2.30A1-310[»]
2DHDX-ray2.13A1-310[»]
2DHEX-ray2.13A1-310[»]
2EDAX-ray2.19A1-310[»]
2EDCX-ray2.30A1-310[»]
2HADX-ray1.90A1-310[»]
2PKYX-ray1.55X1-310[»]
2YXPX-ray1.53X1-310[»]
ProteinModelPortalP22643.
SMRP22643. Positions 1-310.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSS33.990.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00265; UER00387.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
HAMAPMF_01230. Haloalk_dehal_type1.
InterProIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
IPR023489. Haloalkane_dehalogenase_1.
[Graphical view]
PfamPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSPR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMSSF53474. SSF53474. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP22643.

Entry information

Entry nameDHLA_XANAU
AccessionPrimary (citable) accession number: P22643
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways