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P22643

- DHLA_XANAU

UniProt

P22643 - DHLA_XANAU

Protein

Haloalkane dehalogenase

Gene

dhlA

Organism
Xanthobacter autotrophicus
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes terminally mono- and di- chlorinated and brominated alkanes (up to C4 only). The highest activity was found with 1,2-dichloroethane, 1,3-dichloropropane, and 1,2-dibromoethane.

    Catalytic activityi

    1-haloalkane + H2O = a primary alcohol + halide.
    1,2-dichloroethane + H2O = 2-chloroethanol + hydrogen chloride.

    Enzyme regulationi

    Inhibited by thiol reagents such as p-chloromercuribenzoate and iodoacetamide.

    pH dependencei

    Optimum pH is 8.2.

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei124 – 1241Nucleophile
    Binding sitei125 – 1251Halide
    Binding sitei175 – 1751Halide
    Active sitei260 – 2601Proton donor
    Active sitei289 – 2891Proton acceptor

    GO - Molecular functioni

    1. haloalkane dehalogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 1,2-dichloroethane catabolic process Source: UniProtKB-UniPathway
    2. response to toxic substance Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Detoxification

    Enzyme and pathway databases

    UniPathwayiUPA00265; UER00387.

    Protein family/group databases

    MEROPSiS33.990.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Haloalkane dehalogenase (EC:3.8.1.5)
    Gene namesi
    Name:dhlA
    OrganismiXanthobacter autotrophicus
    Taxonomic identifieri280 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 310310Haloalkane dehalogenasePRO_0000216771Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    Secondary structure

    1
    310
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 124
    Beta strandi23 – 275
    Beta strandi35 – 428
    Beta strandi49 – 524
    Helixi60 – 634
    Turni64 – 663
    Helixi67 – 726
    Beta strandi76 – 805
    Beta strandi90 – 923
    Helixi94 – 963
    Helixi99 – 11315
    Beta strandi117 – 1226
    Helixi125 – 1306
    Helixi134 – 1363
    Helixi138 – 1403
    Beta strandi141 – 1488
    Turni155 – 1573
    Helixi160 – 1634
    Turni164 – 1663
    Turni169 – 1713
    Helixi172 – 1809
    Helixi187 – 1948
    Helixi200 – 2078
    Helixi213 – 2153
    Helixi217 – 22711
    Helixi231 – 24616
    Beta strandi250 – 2578
    Beta strandi261 – 2644
    Helixi265 – 27410
    Beta strandi282 – 2843
    Helixi291 – 2944
    Helixi295 – 30814

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1B6GX-ray1.15A3-310[»]
    1BE0X-ray1.96A3-310[»]
    1BEEX-ray2.60A3-310[»]
    1BEZX-ray2.10A3-310[»]
    1CIJX-ray2.30A3-310[»]
    1EDBX-ray2.01A1-310[»]
    1EDDX-ray2.19A1-310[»]
    1EDEX-ray1.90A1-310[»]
    1HDEX-ray2.70A/B1-310[»]
    2DHCX-ray2.30A1-310[»]
    2DHDX-ray2.13A1-310[»]
    2DHEX-ray2.13A1-310[»]
    2EDAX-ray2.19A1-310[»]
    2EDCX-ray2.30A1-310[»]
    2HADX-ray1.90A1-310[»]
    2PKYX-ray1.55X1-310[»]
    2YXPX-ray1.53X1-310[»]
    ProteinModelPortaliP22643.
    SMRiP22643. Positions 1-310.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP22643.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    HAMAPiMF_01230. Haloalk_dehal_type1.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000639. Epox_hydrolase-like.
    IPR023489. Haloalkane_dehalogenase_1.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    [Graphical view]
    PRINTSiPR00111. ABHYDROLASE.
    PR00412. EPOXHYDRLASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P22643-1 [UniParc]FASTAAdd to Basket

    « Hide

    MINAIRTPDQ RFSNLDQYPF SPNYLDDLPG YPGLRAHYLD EGNSDAEDVF    50
    LCLHGEPTWS YLYRKMIPVF AESGARVIAP DFFGFGKSDK PVDEEDYTFE 100
    FHRNFLLALI ERLDLRNITL VVQDWGGFLG LTLPMADPSR FKRLIIMNAC 150
    LMTDPVTQPA FSAFVTQPAD GFTAWKYDLV TPSDLRLDQF MKRWAPTLTE 200
    AEASAYAAPF PDTSYQAGVR KFPKMVAQRD QACIDISTEA ISFWQNDWNG 250
    QTFMAIGMKD KLLGPDVMYP MKALINGCPE PLEIADAGHF VQEFGEQVAR 300
    EALKHFAETE 310
    Length:310
    Mass (Da):35,144
    Last modified:February 1, 1995 - v2
    Checksum:iFB32C94BE5D8940C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26950 Genomic DNA. Translation: AAA88691.1.
    PIRiB43718.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26950 Genomic DNA. Translation: AAA88691.1 .
    PIRi B43718.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1B6G X-ray 1.15 A 3-310 [» ]
    1BE0 X-ray 1.96 A 3-310 [» ]
    1BEE X-ray 2.60 A 3-310 [» ]
    1BEZ X-ray 2.10 A 3-310 [» ]
    1CIJ X-ray 2.30 A 3-310 [» ]
    1EDB X-ray 2.01 A 1-310 [» ]
    1EDD X-ray 2.19 A 1-310 [» ]
    1EDE X-ray 1.90 A 1-310 [» ]
    1HDE X-ray 2.70 A/B 1-310 [» ]
    2DHC X-ray 2.30 A 1-310 [» ]
    2DHD X-ray 2.13 A 1-310 [» ]
    2DHE X-ray 2.13 A 1-310 [» ]
    2EDA X-ray 2.19 A 1-310 [» ]
    2EDC X-ray 2.30 A 1-310 [» ]
    2HAD X-ray 1.90 A 1-310 [» ]
    2PKY X-ray 1.55 X 1-310 [» ]
    2YXP X-ray 1.53 X 1-310 [» ]
    ProteinModelPortali P22643.
    SMRi P22643. Positions 1-310.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S33.990.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00265 ; UER00387 .

    Miscellaneous databases

    EvolutionaryTracei P22643.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    HAMAPi MF_01230. Haloalk_dehal_type1.
    InterProi IPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000639. Epox_hydrolase-like.
    IPR023489. Haloalkane_dehalogenase_1.
    [Graphical view ]
    Pfami PF00561. Abhydrolase_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00111. ABHYDROLASE.
    PR00412. EPOXHYDRLASE.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of 1,2-dichloroethane degradation genes of Xanthobacter autotrophicus GJ10 and expression and sequencing of the dhlA gene."
      Janssen D.B., Pries F., van der Ploeg J., Kazemier B., Terpstra P., Witholt B.
      J. Bacteriol. 171:6791-6799(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: GJ10.
    2. "Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10."
      Keuning S., Janssen D.B., Witholt B.
      J. Bacteriol. 163:635-639(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-10.
    3. "Crystallization of haloalkane dehalogenase from Xanthobacter autotrophicus GJ10."
      Rozeboom H.J., Kingma J., Janssen D.B., Dijkstra B.W.
      J. Mol. Biol. 200:611-612(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: CRYSTALLIZATION.
      Strain: GJ10.
    4. "Crystal structure of haloalkane dehalogenase: an enzyme to detoxify halogenated alkanes."
      Franken S.M., Rozeboom H.J., Kalk K.H., Dijkstra B.W.
      EMBO J. 10:1297-1302(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS), SEQUENCE REVISION TO 120.
      Strain: GJ10.
    5. "Refined X-ray structures of haloalkane dehalogenase at pH 6.2 and pH 8.2 and implications for the reaction mechanism."
      Verschueren K.H.G., Franken S.M., Rozeboom H.J., Kalk K.H., Dijkstra B.W.
      J. Mol. Biol. 232:856-872(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
    6. "Crystallographic analysis of the catalytic mechanism of haloalkane dehalogenase."
      Verschueren K.H.G., Seljee F., Rozeboom H.J., Kalk K.H., Dijkstra B.W.
      Nature 363:693-698(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
      Strain: GJ10.
    7. "Kinetic analysis and X-ray structure of haloalkane dehalogenase with a modified halide-binding site."
      Krooshof G.H., Ridder I.S., Tepper A.W.J.W., Vos G.J., Rozeboom H.J., Kalk K.H., Dijkstra B.W., Janssen D.B.
      Biochemistry 37:15013-15023(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS).
    8. "Crystallographic and kinetic evidence of a collision complex formed during halide import in haloalkane dehalogenase."
      Pikkemaat M.G., Ridder I.S., Rozeboom H.J., Kalk K.H., Dijkstra B.W., Janssen D.B.
      Biochemistry 38:12052-12061(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
    9. "Haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 refined at 1.15 A resolution."
      Ridder I.S., Rozeboom H.J., Dijkstra B.W.
      Acta Crystallogr. D 55:1273-1290(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.15 ANGSTROMS).
      Strain: GJ10.

    Entry informationi

    Entry nameiDHLA_XANAU
    AccessioniPrimary (citable) accession number: P22643
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3