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P22637

- CHOD_BREST

UniProt

P22637 - CHOD_BREST

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Protein
Cholesterol oxidase
Gene
choB
Organism
Brevibacterium sterolicum
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation and isomerization of cholesterol to cholestenone (4-cholesten-3-one), which is an initial step in the cholesterol degradation process.

Catalytic activityi

Cholesterol + O2 = cholest-5-en-3-one + H2O2.
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

Cofactori

FAD.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei406 – 4061Proton acceptor
Active sitei492 – 4921 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi63 – 7917FAD
Add
BLAST

GO - Molecular functioni

  1. cholesterol oxidase activity Source: UniProtKB-EC
  2. flavin adenine dinucleotide binding Source: InterPro
  3. steroid delta-isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. cholesterol metabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Oxidoreductase

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16891.
UniPathwayiUPA00296.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol oxidase (EC:1.1.3.6)
Short name:
CHOD
Alternative name(s):
Cholesterol isomerase (EC:5.3.3.1)
Gene namesi
Name:choB
OrganismiBrevibacterium sterolicum
Taxonomic identifieri1702 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeBrevibacteriaceaeBrevibacterium

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4545Tat-type signal1 Publication
Add
BLAST
Chaini46 – 552507Cholesterol oxidase
PRO_0000012332Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi56 – 627
Helixi66 – 7712
Beta strandi82 – 854
Beta strandi104 – 1063
Beta strandi111 – 1144
Beta strandi140 – 1456
Beta strandi150 – 1545
Helixi159 – 1624
Helixi173 – 1797
Helixi185 – 1906
Helixi192 – 2009
Helixi207 – 2126
Helixi214 – 2163
Helixi217 – 22812
Beta strandi233 – 2353
Beta strandi238 – 2403
Helixi242 – 2498
Turni257 – 2604
Beta strandi267 – 2704
Turni273 – 2764
Helixi277 – 2837
Beta strandi287 – 2904
Beta strandi292 – 3009
Beta strandi302 – 31312
Beta strandi319 – 33214
Helixi335 – 34814
Turni357 – 3604
Beta strandi368 – 3747
Beta strandi391 – 3944
Beta strandi403 – 4086
Beta strandi418 – 4258
Beta strandi433 – 4364
Turni437 – 4404
Beta strandi441 – 4444
Helixi448 – 4514
Helixi452 – 46918
Beta strandi484 – 4863
Beta strandi488 – 4914
Turni499 – 5013
Beta strandi515 – 5173
Helixi520 – 5223
Helixi532 – 54918

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1COYX-ray1.80A46-552[»]
3COXX-ray1.80A46-552[»]
ProteinModelPortaliP22637.
SMRiP22637. Positions 49-551.

Miscellaneous databases

EvolutionaryTraceiP22637.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view]
PROSITEiPS00623. GMC_OXRED_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22637-1 [UniParc]FASTAAdd to Basket

« Hide

MTDSRANRAD ATRGVASVSR RRFLAGAGLT AGAIALSSMS TSASAAPSRT    50
LADGDRVPAL VIGSGYGGAV AALRLTQAGI PTQIVEMGRS WDTPGSDGKI 100
FCGMLNPDKR SMWLADKTDQ PVSNFMGFGI NKSIDRYVGV LDSERFSGIK 150
VYQGRGVGGG SLVNGGMAVT PKRNYFEEIL PSVDSNEMYN KYFPRANTGL 200
GVNNIDQAWF ESTEWYKFAR TGRKTAQRSG FTTAFVPNVY DFEYMKKEAA 250
GQVTKSGLGG EVIYGNNAGK KSLDKTYLAQ AAATGKLTIT TLHRVTKVAP 300
ATGSGYSVTM EQIDEQGNVV ATKVVTADRV FFAAGSVGTS KLLVSMKAQG 350
HLPNLSSQVG EGWGNNGNIM VGRANHMWDA TGSKQATIPT MGIDNWADPT 400
APIFAEIAPL PAGLETYVSL YLAITKNPER ARFQFNSGTG KVDLTWAQSQ 450
NQKGIDMAKK VFDKINQKEG TIYRTDLFGV YFKTWGDDFT YHPLGGVLLN 500
KATDNFGRLP EYPGLYVVDG SLVPGNVGVN PFVTITRLAE RNMDKIISSD 550
IQ 552
Length:552
Mass (Da):59,358
Last modified:May 1, 1992 - v2
Checksum:i74913FAED74B4F09
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00712 Genomic DNA. Translation: BAA00617.1.
PIRiJQ1193.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D00712 Genomic DNA. Translation: BAA00617.1 .
PIRi JQ1193.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1COY X-ray 1.80 A 46-552 [» ]
3COX X-ray 1.80 A 46-552 [» ]
ProteinModelPortali P22637.
SMRi P22637. Positions 49-551.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00296 .
BioCyci MetaCyc:MONOMER-16891.

Miscellaneous databases

EvolutionaryTracei P22637.

Family and domain databases

InterProi IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view ]
PROSITEi PS00623. GMC_OXRED_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of gene choB encoding cholesterol oxidase of Brevibacterium sterolicum: comparison with choA of streptomyces sp. SA-COO."
    Ohta T., Fujishiro K., Yamaguchi K., Tamura Y., Aisaka K., Uwajima T., Hasegawa M.
    Gene 103:93-96(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 46-55.
    Strain: ATCC 21387 / NCIB 11161.
  2. "Isolation and identification of the gene of cholesterol oxidase from Brevibacterium sterolicum ATCC 21387, a widely used enzyme in clinical analysis."
    Fujishiro K., Ota T., Hasegawa M., Yamaguchi K., Mizukami T., Uwajima T.
    Biochem. Biophys. Res. Commun. 172:721-727(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-552, PARTIAL PROTEIN SEQUENCE.
    Strain: ATCC 21387 / NCIB 11161.
  3. Erratum
    Fujishiro K., Ota T., Hasegawa M., Yamaguchi K., Mizukami T., Uwajima T.
    Biochem. Biophys. Res. Commun. 173:1384-1384(1990)
  4. "Crystal structure of cholesterol oxidase from Brevibacterium sterolicum refined at 1.8-A resolution."
    Vrielink A., Lloyld L.F., Blow D.M.
    J. Mol. Biol. 219:533-554(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

Entry informationi

Entry nameiCHOD_BREST
AccessioniPrimary (citable) accession number: P22637
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: May 1, 1992
Last modified: February 19, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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