Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P22637 (CHOD_BREST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cholesterol oxidase

Short name=CHOD
EC=1.1.3.6
Alternative name(s):
Cholesterol isomerase
EC=5.3.3.1
Gene names
Name:choB
OrganismBrevibacterium sterolicum
Taxonomic identifier1702 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicrococcineaeBrevibacteriaceaeBrevibacterium

Protein attributes

Sequence length552 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the oxidation and isomerization of cholesterol to cholestenone (4-cholesten-3-one), which is an initial step in the cholesterol degradation process.

Catalytic activity

Cholesterol + O2 = cholest-5-en-3-one + H2O2.

A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

Cofactor

FAD.

Pathway

Steroid metabolism; cholesterol metabolism.

Subcellular location

Secreted.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Sequence similarities

Belongs to the GMC oxidoreductase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4545Tat-type signal Ref.1
Chain46 – 552507Cholesterol oxidase
PRO_0000012332

Regions

Nucleotide binding63 – 7917FAD

Sites

Active site4061Proton acceptor
Active site4921 By similarity

Secondary structure

................................................................................ 552
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P22637 [UniParc].

Last modified May 1, 1992. Version 2.
Checksum: 74913FAED74B4F09

FASTA55259,358
        10         20         30         40         50         60 
MTDSRANRAD ATRGVASVSR RRFLAGAGLT AGAIALSSMS TSASAAPSRT LADGDRVPAL 

        70         80         90        100        110        120 
VIGSGYGGAV AALRLTQAGI PTQIVEMGRS WDTPGSDGKI FCGMLNPDKR SMWLADKTDQ 

       130        140        150        160        170        180 
PVSNFMGFGI NKSIDRYVGV LDSERFSGIK VYQGRGVGGG SLVNGGMAVT PKRNYFEEIL 

       190        200        210        220        230        240 
PSVDSNEMYN KYFPRANTGL GVNNIDQAWF ESTEWYKFAR TGRKTAQRSG FTTAFVPNVY 

       250        260        270        280        290        300 
DFEYMKKEAA GQVTKSGLGG EVIYGNNAGK KSLDKTYLAQ AAATGKLTIT TLHRVTKVAP 

       310        320        330        340        350        360 
ATGSGYSVTM EQIDEQGNVV ATKVVTADRV FFAAGSVGTS KLLVSMKAQG HLPNLSSQVG 

       370        380        390        400        410        420 
EGWGNNGNIM VGRANHMWDA TGSKQATIPT MGIDNWADPT APIFAEIAPL PAGLETYVSL 

       430        440        450        460        470        480 
YLAITKNPER ARFQFNSGTG KVDLTWAQSQ NQKGIDMAKK VFDKINQKEG TIYRTDLFGV 

       490        500        510        520        530        540 
YFKTWGDDFT YHPLGGVLLN KATDNFGRLP EYPGLYVVDG SLVPGNVGVN PFVTITRLAE 

       550 
RNMDKIISSD IQ 

« Hide

References

[1]"Sequence of gene choB encoding cholesterol oxidase of Brevibacterium sterolicum: comparison with choA of streptomyces sp. SA-COO."
Ohta T., Fujishiro K., Yamaguchi K., Tamura Y., Aisaka K., Uwajima T., Hasegawa M.
Gene 103:93-96(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 46-55.
Strain: ATCC 21387 / NCIB 11161.
[2]"Isolation and identification of the gene of cholesterol oxidase from Brevibacterium sterolicum ATCC 21387, a widely used enzyme in clinical analysis."
Fujishiro K., Ota T., Hasegawa M., Yamaguchi K., Mizukami T., Uwajima T.
Biochem. Biophys. Res. Commun. 172:721-727(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-552, PARTIAL PROTEIN SEQUENCE.
Strain: ATCC 21387 / NCIB 11161.
[3]Erratum
Fujishiro K., Ota T., Hasegawa M., Yamaguchi K., Mizukami T., Uwajima T.
Biochem. Biophys. Res. Commun. 173:1384-1384(1990)
[4]"Crystal structure of cholesterol oxidase from Brevibacterium sterolicum refined at 1.8-A resolution."
Vrielink A., Lloyld L.F., Blow D.M.
J. Mol. Biol. 219:533-554(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D00712 Genomic DNA. Translation: BAA00617.1.
PIRJQ1193.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1COYX-ray1.80A46-552[»]
3COXX-ray1.80A46-552[»]
ProteinModelPortalP22637.
SMRP22637. Positions 49-551.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16891.
UniPathwayUPA00296.

Family and domain databases

InterProIPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
IPR006311. TAT_signal.
[Graphical view]
PfamPF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view]
PROSITEPS00623. GMC_OXRED_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP22637.

Entry information

Entry nameCHOD_BREST
AccessionPrimary (citable) accession number: P22637
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: May 1, 1992
Last modified: February 19, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways