P22636 (PCYB_PSEPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protocatechuate 4,5-dioxygenase beta chain
EC=1.13.11.8

Alternative name(s):
4,5-PCD

Gene names

Name:

ligB

Organism

Pseudomonas paucimobilis (Sphingomonas paucimobilis)

Taxonomic identifier

13689 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingomonas

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Responsible for the aromatic ring fission of protocatechuate.
Catalytic activity	Protocatechuate + O2 = 4-carboxy-2-hydroxymuconate semialdehyde.
Cofactor	Fe2+ ion.
Subunit structure	Composed of two subunits (alpha and beta) in a 1:1 ratio.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ferrous iron binding Inferred from electronic annotation. Source: InterPro protocatechuate 4,5-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 302

302

Protocatechuate 4,5-dioxygenase beta chain

PRO_0000085101

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

302

Helix Strand Turn

Details...

Beta strand

3 – 10

8

Helix

14 – 21

8

Turn

28 – 30

3

Helix

31 – 44

14

Turn

47 – 49

3

Beta strand

52 – 58

7

Beta strand

62 – 64

3

Beta strand

69 – 76

8

Beta strand

78 – 81

4

Beta strand

87 – 90

4

Helix

100 – 112

13

Beta strand

118 – 122

5

Helix

127 – 137

11

Beta strand

144 – 152

9

Beta strand

155 – 157

3

Helix

162 – 177

16

Beta strand

179 – 181

3

Beta strand

184 – 190

7

Turn

200 – 203

4

Helix

207 – 219

13

Helix

221 – 224

4

Helix

229 – 236

8

Helix

240 – 243

4

Helix

244 – 251

8

Beta strand

257 – 268

12

Beta strand

271 – 280

10

Helix

281 – 283

3

Beta strand

289 – 296

8

Sequences

	Sequence		Length	Mass (Da)
	P22636 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 0552B3B0E59702E5	FASTA	302	33,292
	10 20 30 40 50 60 MARVTTGITS SHIPALGAAI QTGTSDNDYW GPVFKGYQPI RDWIKQPGNM PDVVILVYND 70 80 90 100 110 120 HASAFDMNII PTFAIGCAET FKPADEGWGP RPVPDVKGHP DLAWHIAQSL ILDEFDMTIM 130 140 150 160 170 180 NQMDVDHGCT VPLSMIFGEP EEWPCKVIPF PVNVVTYPPP SGKRCFALGD SIRAAVESFP 190 200 210 220 230 240 EDLNVHVWGT GGMSHQLQGP RAGLINKEFD LNFIDKLISD PEELSKMPHI QYLRESGSEG 250 260 270 280 290 300 VELVMWLIMR GALPEKVRDL YTFYHIPASN TALGAMILQP EETAGTPLEP RKVMSGHSLA QA

References

[1]	"Molecular cloning of the protocatechuate 4,5-dioxygenase genes of Pseudomonas paucimobilis." Noda Y., Nishikawa S., Shiozuka K., Kadokura H., Nakajima H., Yoda K., Katayama Y., Morohoshi N., Haraguchi T., Yamasaki M. J. Bacteriol. 172:2704-2709(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-9. Strain: SYK-6.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M34835 Unassigned DNA. Translation: AAA17728.1.

PIR

B35271.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B4U	X-ray	2.20	B/D	1-302	[»]
1BOU	X-ray	2.20	B/D	1-302	[»]

ProteinModelPortal

P22636.

SMR

P22636. Positions 2-299.

ModBase

Search...

Protein-protein interaction databases

IntAct

P22636. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-15117.

Family and domain databases

Gene3D

3.40.830.10. 1 hit.

InterPro

IPR004183. Xdiol_dOase_suB.
[Graphical view]

Pfam

PF02900. LigB. 1 hit.
[Graphical view]

SUPFAM

SSF53213. Xdiol_dOase_3B. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P22636.

Entry information

Entry name

PCYB_PSEPA

Accession

Primary (citable) accession number: P22636

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program