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P22636

- PCYB_SPHPI

UniProt

P22636 - PCYB_SPHPI

Protein

Protocatechuate 4,5-dioxygenase beta chain

Gene

ligB

Organism
Sphingomonas paucimobilis (Pseudomonas paucimobilis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 62 (11 Dec 2013)
      Sequence version 1 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    Responsible for the aromatic ring fission of protocatechuate.

    Catalytic activityi

    Protocatechuate + O2 = 4-carboxy-2-hydroxymuconate semialdehyde.

    Cofactori

    Fe2+ ion.

    GO - Molecular functioni

    1. ferrous iron binding Source: InterPro
    2. protocatechuate 4,5-dioxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. aromatic compound catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Biological processi

    Aromatic hydrocarbons catabolism

    Keywords - Ligandi

    Iron

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15117.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protocatechuate 4,5-dioxygenase beta chain (EC:1.13.11.8)
    Alternative name(s):
    4,5-PCD
    Gene namesi
    Name:ligB
    OrganismiSphingomonas paucimobilis (Pseudomonas paucimobilis)
    Taxonomic identifieri13689 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 302302Protocatechuate 4,5-dioxygenase beta chainPRO_0000085101Add
    BLAST

    Interactioni

    Subunit structurei

    Composed of two subunits (alpha and beta) in a 1:1 ratio.

    Protein-protein interaction databases

    IntActiP22636. 1 interaction.

    Structurei

    Secondary structure

    1
    302
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 108
    Helixi14 – 218
    Turni28 – 303
    Helixi31 – 4414
    Turni47 – 493
    Beta strandi52 – 587
    Beta strandi62 – 643
    Beta strandi69 – 768
    Beta strandi78 – 814
    Beta strandi87 – 904
    Helixi100 – 11213
    Beta strandi118 – 1225
    Helixi127 – 13711
    Beta strandi144 – 1529
    Beta strandi155 – 1573
    Helixi162 – 17716
    Beta strandi179 – 1813
    Beta strandi184 – 1907
    Turni200 – 2034
    Helixi207 – 21913
    Helixi221 – 2244
    Helixi229 – 2368
    Helixi240 – 2434
    Helixi244 – 2518
    Beta strandi257 – 26812
    Beta strandi271 – 28010
    Helixi281 – 2833
    Beta strandi289 – 2968

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1B4UX-ray2.20B/D1-302[»]
    1BOUX-ray2.20B/D1-302[»]
    ProteinModelPortaliP22636.
    SMRiP22636. Positions 2-299.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP22636.

    Family & Domainsi

    Family and domain databases

    Gene3Di3.40.830.10. 1 hit.
    InterProiIPR004183. Xdiol_dOase_suB.
    [Graphical view]
    PfamiPF02900. LigB. 1 hit.
    [Graphical view]
    SUPFAMiSSF53213. SSF53213. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P22636-1 [UniParc]FASTAAdd to Basket

    « Hide

    MARVTTGITS SHIPALGAAI QTGTSDNDYW GPVFKGYQPI RDWIKQPGNM    50
    PDVVILVYND HASAFDMNII PTFAIGCAET FKPADEGWGP RPVPDVKGHP 100
    DLAWHIAQSL ILDEFDMTIM NQMDVDHGCT VPLSMIFGEP EEWPCKVIPF 150
    PVNVVTYPPP SGKRCFALGD SIRAAVESFP EDLNVHVWGT GGMSHQLQGP 200
    RAGLINKEFD LNFIDKLISD PEELSKMPHI QYLRESGSEG VELVMWLIMR 250
    GALPEKVRDL YTFYHIPASN TALGAMILQP EETAGTPLEP RKVMSGHSLA 300
    QA 302
    Length:302
    Mass (Da):33,292
    Last modified:August 1, 1991 - v1
    Checksum:i0552B3B0E59702E5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34835 Unassigned DNA. Translation: AAA17728.1.
    PIRiB35271.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M34835 Unassigned DNA. Translation: AAA17728.1 .
    PIRi B35271.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1B4U X-ray 2.20 B/D 1-302 [» ]
    1BOU X-ray 2.20 B/D 1-302 [» ]
    ProteinModelPortali P22636.
    SMRi P22636. Positions 2-299.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P22636. 1 interaction.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-15117.

    Miscellaneous databases

    EvolutionaryTracei P22636.

    Family and domain databases

    Gene3Di 3.40.830.10. 1 hit.
    InterProi IPR004183. Xdiol_dOase_suB.
    [Graphical view ]
    Pfami PF02900. LigB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53213. SSF53213. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the protocatechuate 4,5-dioxygenase genes of Pseudomonas paucimobilis."
      Noda Y., Nishikawa S., Shiozuka K., Kadokura H., Nakajima H., Yoda K., Katayama Y., Morohoshi N., Haraguchi T., Yamasaki M.
      J. Bacteriol. 172:2704-2709(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-9.
      Strain: SYK-6.

    Entry informationi

    Entry nameiPCYB_SPHPI
    AccessioniPrimary (citable) accession number: P22636
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1991
    Last sequence update: August 1, 1991
    Last modified: December 11, 2013
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3