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P22636 (PCYB_SPHPI) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protocatechuate 4,5-dioxygenase beta chain

EC=1.13.11.8
Alternative name(s):
4,5-PCD
Gene names
Name:ligB
OrganismSphingomonas paucimobilis (Pseudomonas paucimobilis)
Taxonomic identifier13689 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas

Protein attributes

Sequence length302 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for the aromatic ring fission of protocatechuate.

Catalytic activity

Protocatechuate + O2 = 4-carboxy-2-hydroxymuconate semialdehyde.

Cofactor

Fe2+ ion.

Subunit structure

Composed of two subunits (alpha and beta) in a 1:1 ratio.

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   LigandIron
   Molecular functionDioxygenase
Oxidoreductase
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processaromatic compound catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionferrous iron binding

Inferred from electronic annotation. Source: InterPro

protocatechuate 4,5-dioxygenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 302302Protocatechuate 4,5-dioxygenase beta chain
PRO_0000085101

Secondary structure

...................................................... 302
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P22636 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: 0552B3B0E59702E5

FASTA30233,292
        10         20         30         40         50         60 
MARVTTGITS SHIPALGAAI QTGTSDNDYW GPVFKGYQPI RDWIKQPGNM PDVVILVYND 

        70         80         90        100        110        120 
HASAFDMNII PTFAIGCAET FKPADEGWGP RPVPDVKGHP DLAWHIAQSL ILDEFDMTIM 

       130        140        150        160        170        180 
NQMDVDHGCT VPLSMIFGEP EEWPCKVIPF PVNVVTYPPP SGKRCFALGD SIRAAVESFP 

       190        200        210        220        230        240 
EDLNVHVWGT GGMSHQLQGP RAGLINKEFD LNFIDKLISD PEELSKMPHI QYLRESGSEG 

       250        260        270        280        290        300 
VELVMWLIMR GALPEKVRDL YTFYHIPASN TALGAMILQP EETAGTPLEP RKVMSGHSLA 


QA 

« Hide

References

[1]"Molecular cloning of the protocatechuate 4,5-dioxygenase genes of Pseudomonas paucimobilis."
Noda Y., Nishikawa S., Shiozuka K., Kadokura H., Nakajima H., Yoda K., Katayama Y., Morohoshi N., Haraguchi T., Yamasaki M.
J. Bacteriol. 172:2704-2709(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-9.
Strain: SYK-6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M34835 Unassigned DNA. Translation: AAA17728.1.
PIRB35271.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B4UX-ray2.20B/D1-302[»]
1BOUX-ray2.20B/D1-302[»]
ProteinModelPortalP22636.
SMRP22636. Positions 2-299.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP22636. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-15117.

Family and domain databases

Gene3D3.40.830.10. 1 hit.
InterProIPR004183. Xdiol_dOase_suB.
[Graphical view]
PfamPF02900. LigB. 1 hit.
[Graphical view]
SUPFAMSSF53213. SSF53213. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP22636.

Entry information

Entry namePCYB_SPHPI
AccessionPrimary (citable) accession number: P22636
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: December 11, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references