ID   PCYA_SPHSK              Reviewed;         139 AA.
AC   P22635;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   13-SEP-2023, entry version 90.
DE   RecName: Full=Protocatechuate 4,5-dioxygenase alpha chain;
DE            EC=1.13.11.8;
DE   AltName: Full=4,5-PCD;
GN   Name=ligA;
OS   Sphingobium sp. (strain NBRC 103272 / SYK-6).
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingobium.
OX   NCBI_TaxID=627192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 23-34.
RC   STRAIN=NBRC 103272 / SYK-6;
RX   PubMed=2185230; DOI=10.1128/jb.172.5.2704-2709.1990;
RA   Noda Y., Nishikawa S., Shiozuka K., Kadokura H., Nakajima H., Yoda K.,
RA   Katayama Y., Morohoshi N., Haraguchi T., Yamasaki M.;
RT   "Molecular cloning of the protocatechuate 4,5-dioxygenase genes of
RT   Pseudomonas paucimobilis.";
RL   J. Bacteriol. 172:2704-2709(1990).
CC   -!- FUNCTION: Responsible for the aromatic ring fission of protocatechuate.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3,4-dihydroxybenzoate + O2 = 4-carboxy-2-hydroxy-cis,cis-
CC         muconate 6-semialdehyde + H(+); Xref=Rhea:RHEA:24044,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:36241,
CC         ChEBI:CHEBI:58358; EC=1.13.11.8;
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC   -!- SUBUNIT: Composed of two subunits (alpha and beta) in a 1:1 ratio.
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DR   EMBL; M34835; AAA17727.1; -; Unassigned_DNA.
DR   PIR; A35271; A35271.
DR   RefSeq; WP_014075577.1; NC_015976.1.
DR   PDB; 1B4U; X-ray; 2.20 A; A/C=1-139.
DR   PDB; 1BOU; X-ray; 2.20 A; A/C=1-139.
DR   PDBsum; 1B4U; -.
DR   PDBsum; 1BOU; -.
DR   AlphaFoldDB; P22635; -.
DR   SMR; P22635; -.
DR   IntAct; P22635; 1.
DR   STRING; 627192.SLG_12510; -.
DR   KEGG; ag:AAA17727; -.
DR   OrthoDB; 7864521at2; -.
DR   BioCyc; MetaCyc:MONOMER-15116; -.
DR   EvolutionaryTrace; P22635; -.
DR   GO; GO:0018579; F:protocatechuate 4,5-dioxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd07924; PCA_45_Doxase_A; 1.
DR   Gene3D; 1.10.700.10; Dioxygenase LigAB, LigA subunit; 1.
DR   InterPro; IPR036622; LigA_sf.
DR   InterPro; IPR014159; PCA_LigA.
DR   InterPro; IPR011986; Xdiol_dOase_LigA.
DR   NCBIfam; TIGR02792; PCA_ligA; 1.
DR   Pfam; PF07746; LigA; 1.
DR   SUPFAM; SSF48076; LigA subunit of an aromatic-ring-opening dioxygenase LigAB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Aromatic hydrocarbons catabolism; Dioxygenase;
KW   Direct protein sequencing; Iron; Oxidoreductase.
FT   CHAIN           1..139
FT                   /note="Protocatechuate 4,5-dioxygenase alpha chain"
FT                   /id="PRO_0000085100"
FT   HELIX           10..15
FT                   /evidence="ECO:0007829|PDB:1B4U"
FT   HELIX           16..19
FT                   /evidence="ECO:0007829|PDB:1B4U"
FT   HELIX           28..33
FT                   /evidence="ECO:0007829|PDB:1B4U"
FT   HELIX           35..43
FT                   /evidence="ECO:0007829|PDB:1B4U"
FT   HELIX           47..55
FT                   /evidence="ECO:0007829|PDB:1B4U"
FT   HELIX           57..62
FT                   /evidence="ECO:0007829|PDB:1B4U"
FT   TURN            63..65
FT                   /evidence="ECO:0007829|PDB:1B4U"
FT   HELIX           68..76
FT                   /evidence="ECO:0007829|PDB:1B4U"
FT   HELIX           79..84
FT                   /evidence="ECO:0007829|PDB:1B4U"
FT   HELIX           89..98
FT                   /evidence="ECO:0007829|PDB:1B4U"
FT   HELIX           103..109
FT                   /evidence="ECO:0007829|PDB:1B4U"
FT   TURN            110..112
FT                   /evidence="ECO:0007829|PDB:1B4U"
FT   HELIX           115..123
FT                   /evidence="ECO:0007829|PDB:1B4U"
FT   TURN            135..137
FT                   /evidence="ECO:0007829|PDB:1B4U"
SQ   SEQUENCE   139 AA;  15549 MW;  E6CFEF0499A3FD26 CRC64;
     MTEKKERIDV HAYLAEFDDI PGTRVFTAQR ARKGYNLNQF AMSLMKAENR ERFKADESAY
     LDEWNLTPAA KAAVLARDYN AMIDEGGNVY FLSKLFSTDG KSFQFAAGSM TGMTQEEYAQ
     MMIDGGRSPA GVRSIKGGY
//