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P22635 (PCYA_SPHPI) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protocatechuate 4,5-dioxygenase alpha chain

EC=1.13.11.8
Alternative name(s):
4,5-PCD
Gene names
Name:ligA
OrganismSphingomonas paucimobilis (Pseudomonas paucimobilis)
Taxonomic identifier13689 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas

Protein attributes

Sequence length139 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Responsible for the aromatic ring fission of protocatechuate.

Catalytic activity

Protocatechuate + O2 = 4-carboxy-2-hydroxymuconate semialdehyde.

Cofactor

Fe2+ ion.

Subunit structure

Composed of two subunits (alpha and beta) in a 1:1 ratio.

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   LigandIron
   Molecular functionDioxygenase
Oxidoreductase
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological_processaromatic compound catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionprotocatechuate 4,5-dioxygenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 139139Protocatechuate 4,5-dioxygenase alpha chain
PRO_0000085100

Secondary structure

.......................... 139
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P22635 [UniParc].

Last modified August 1, 1991. Version 1.
Checksum: E6CFEF0499A3FD26

FASTA13915,549
        10         20         30         40         50         60 
MTEKKERIDV HAYLAEFDDI PGTRVFTAQR ARKGYNLNQF AMSLMKAENR ERFKADESAY 

        70         80         90        100        110        120 
LDEWNLTPAA KAAVLARDYN AMIDEGGNVY FLSKLFSTDG KSFQFAAGSM TGMTQEEYAQ 

       130 
MMIDGGRSPA GVRSIKGGY 

« Hide

References

[1]"Molecular cloning of the protocatechuate 4,5-dioxygenase genes of Pseudomonas paucimobilis."
Noda Y., Nishikawa S., Shiozuka K., Kadokura H., Nakajima H., Yoda K., Katayama Y., Morohoshi N., Haraguchi T., Yamasaki M.
J. Bacteriol. 172:2704-2709(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-34.
Strain: SYK-6.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M34835 Unassigned DNA. Translation: AAA17727.1.
PIRA35271.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B4UX-ray2.20A/C1-139[»]
1BOUX-ray2.20A/C1-139[»]
ProteinModelPortalP22635.
SMRP22635. Positions 8-139.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP22635. 1 interaction.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-15116.

Family and domain databases

Gene3D1.10.700.10. 1 hit.
InterProIPR014159. PCA_ligA.
IPR011986. Xdiol_dOase_LigA.
[Graphical view]
PfamPF07746. LigA. 1 hit.
[Graphical view]
SUPFAMSSF48076. SSF48076. 1 hit.
TIGRFAMsTIGR02792. PCA_ligA. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP22635.

Entry information

Entry namePCYA_SPHPI
AccessionPrimary (citable) accession number: P22635
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: December 11, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references