Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protocatechuate 4,5-dioxygenase alpha chain

Gene

ligA

Organism
Sphingomonas paucimobilis (Pseudomonas paucimobilis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the aromatic ring fission of protocatechuate.

Catalytic activityi

Protocatechuate + O2 = 4-carboxy-2-hydroxymuconate semialdehyde.

Cofactori

GO - Molecular functioni

  1. protocatechuate 4,5-dioxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. aromatic compound catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15116.

Names & Taxonomyi

Protein namesi
Recommended name:
Protocatechuate 4,5-dioxygenase alpha chain (EC:1.13.11.8)
Alternative name(s):
4,5-PCD
Gene namesi
Name:ligA
OrganismiSphingomonas paucimobilis (Pseudomonas paucimobilis)
Taxonomic identifieri13689 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaSphingomonadalesSphingomonadaceaeSphingomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 139139Protocatechuate 4,5-dioxygenase alpha chainPRO_0000085100Add
BLAST

Interactioni

Subunit structurei

Composed of two subunits (alpha and beta) in a 1:1 ratio.

Protein-protein interaction databases

IntActiP22635. 1 interaction.

Structurei

Secondary structure

1
139
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 156Combined sources
Helixi16 – 194Combined sources
Helixi28 – 336Combined sources
Helixi35 – 439Combined sources
Helixi47 – 559Combined sources
Helixi57 – 626Combined sources
Turni63 – 653Combined sources
Helixi68 – 769Combined sources
Helixi79 – 846Combined sources
Helixi89 – 9810Combined sources
Helixi103 – 1097Combined sources
Turni110 – 1123Combined sources
Helixi115 – 1239Combined sources
Turni135 – 1373Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B4UX-ray2.20A/C1-139[»]
1BOUX-ray2.20A/C1-139[»]
SMRiP22635. Positions 8-139.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22635.

Family & Domainsi

Family and domain databases

Gene3Di1.10.700.10. 1 hit.
InterProiIPR014159. PCA_ligA.
IPR011986. Xdiol_dOase_LigA.
[Graphical view]
PfamiPF07746. LigA. 1 hit.
[Graphical view]
SUPFAMiSSF48076. SSF48076. 1 hit.
TIGRFAMsiTIGR02792. PCA_ligA. 1 hit.

Sequencei

Sequence statusi: Complete.

P22635-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEKKERIDV HAYLAEFDDI PGTRVFTAQR ARKGYNLNQF AMSLMKAENR
60 70 80 90 100
ERFKADESAY LDEWNLTPAA KAAVLARDYN AMIDEGGNVY FLSKLFSTDG
110 120 130
KSFQFAAGSM TGMTQEEYAQ MMIDGGRSPA GVRSIKGGY
Length:139
Mass (Da):15,549
Last modified:July 31, 1991 - v1
Checksum:iE6CFEF0499A3FD26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34835 Unassigned DNA. Translation: AAA17727.1.
PIRiA35271.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34835 Unassigned DNA. Translation: AAA17727.1.
PIRiA35271.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B4UX-ray2.20A/C1-139[»]
1BOUX-ray2.20A/C1-139[»]
SMRiP22635. Positions 8-139.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP22635. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15116.

Miscellaneous databases

EvolutionaryTraceiP22635.

Family and domain databases

Gene3Di1.10.700.10. 1 hit.
InterProiIPR014159. PCA_ligA.
IPR011986. Xdiol_dOase_LigA.
[Graphical view]
PfamiPF07746. LigA. 1 hit.
[Graphical view]
SUPFAMiSSF48076. SSF48076. 1 hit.
TIGRFAMsiTIGR02792. PCA_ligA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of the protocatechuate 4,5-dioxygenase genes of Pseudomonas paucimobilis."
    Noda Y., Nishikawa S., Shiozuka K., Kadokura H., Nakajima H., Yoda K., Katayama Y., Morohoshi N., Haraguchi T., Yamasaki M.
    J. Bacteriol. 172:2704-2709(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-34.
    Strain: SYK-6.

Entry informationi

Entry nameiPCYA_SPHPI
AccessioniPrimary (citable) accession number: P22635
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 31, 1991
Last sequence update: July 31, 1991
Last modified: March 31, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.