Reviewed, UniProtKB/Swiss-Prot P22635 (PCYA_PSEPA)

Names and origin

Protein names

Recommended name:
    Protocatechuate 4,5-dioxygenase alpha chain
    EC=1.13.11.8
Alternative name(s):
    4,5-PCD

Gene names

Name:

ligA

Organism

Pseudomonas paucimobilis (Sphingomonas paucimobilis)

Taxonomic identifier

13689 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingomonas

Protein attributes

Sequence length	139 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for the aromatic ring fission of protocatechuate.
Catalytic activity	Protocatechuate + O2 = 4-carboxy-2-hydroxymuconate semialdehyde.
Cofactor	Fe2+ ion.
Subunit structure	Composed of two subunits (alpha and beta) in a 1:1 ratio.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protocatechuate 4,5-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 139

139

Protocatechuate 4,5-dioxygenase alpha chain

PRO_0000085100

Secondary structure

1

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139

Helix Strand Turn

Details...

Helix

10 – 15

6

Helix

16 – 19

4

Helix

28 – 33

6

Helix

35 – 43

9

Helix

47 – 55

9

Helix

57 – 62

6

Turn

63 – 65

3

Helix

68 – 76

9

Helix

79 – 84

6

Helix

89 – 98

10

Helix

103 – 109

7

Turn

110 – 112

3

Helix

115 – 123

9

Turn

135 – 137

3

Sequences

	Sequence		Length	Mass (Da)
	P22635-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: E6CFEF0499A3FD26	FASTA	139	15,549
	10 20 30 40 50 60 MTEKKERIDV HAYLAEFDDI PGTRVFTAQR ARKGYNLNQF AMSLMKAENR ERFKADESAY 70 80 90 100 110 120 LDEWNLTPAA KAAVLARDYN AMIDEGGNVY FLSKLFSTDG KSFQFAAGSM TGMTQEEYAQ 130 MMIDGGRSPA GVRSIKGGY

References

[1]	"Molecular cloning of the protocatechuate 4,5-dioxygenase genes of Pseudomonas paucimobilis." Noda Y., Nishikawa S., Shiozuka K., Kadokura H., Nakajima H., Yoda K., Katayama Y., Morohoshi N., Haraguchi T., Yamasaki M. J. Bacteriol. 172:2704-2709(1990) [PubMed: 2185230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 23-34. Strain: SYK-6.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M34835 Unassigned DNA. Translation: AAA17727.1.

PIR

A35271.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B4U	X-ray	2.20	A/C	1-139	[»]
1BOU	X-ray	2.20	A/C	1-139	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

1.13.11.8. 97493.

Family and domain databases

InterPro

IPR014159. PCA_ligA.
IPR011986. Xdiol_dOase_LigA.
[Graphical view]

Gene3D

G3DSA:1.10.700.10. Xdiol_dOase_LigA. 1 hit.

Pfam

PF07746. LigA. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02792. PCA_ligA. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PCYA_PSEPA

Accession

Primary (citable) accession number: P22635

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	January 20, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)