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P22634 (MURI_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamate racemase

EC=5.1.1.3
Gene names
Name:murI
Synonyms:dga, glr, yijA
Ordered Locus Names:b3967, JW5550
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Provides the (R)-glutamate required for cell wall biosynthesis. HAMAP-Rule MF_00258

Catalytic activity

L-glutamate = D-glutamate. HAMAP-Rule MF_00258

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00258

Sequence similarities

Belongs to the aspartate/glutamate racemases family.

Sequence caution

The sequence AAA23677.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence AAC43073.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence CAA23637.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence CAA23638.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

tufAP0CE471EBI-554903,EBI-301077

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 285285Glutamate racemase HAMAP-Rule MF_00258
PRO_0000095470

Secondary structure

.................................................. 285
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P22634 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: EC87281B577B8E93

FASTA28531,002
        10         20         30         40         50         60 
MATKLQDGNT PCLAATPSEP RPTVLVFDSG VGGLSVYDEI RHLLPDLHYI YAFDNVAFPY 

        70         80         90        100        110        120 
GEKSEAFIVE RVVAIVTAVQ ERYPLALAVV ACNTASTVSL PALREKFDFP VVGVVPAIKP 

       130        140        150        160        170        180 
AARLTANGIV GLLATRGTVK RSYTHELIAR FANECQIEML GSAEMVELAE AKLHGEDVSL 

       190        200        210        220        230        240 
DALKRILRPW LRMKEPPDTV VLGCTHFPLL QEELLQVLPE GTRLVDSGAA IARRTAWLLE 

       250        260        270        280 
HEAPDAKSAD ANIAFCMAMT PGAEQLLPVL QRYGFETLEK LAVLG 

« Hide

References

« Hide 'large scale' references
[1]"Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli."
Brosius J., Dull T.J., Sleeter D.D., Noller H.F.
J. Mol. Biol. 148:107-127(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Identification of two new promoters probably involved in the transcription of a ribosomal RNA gene of Escherichia coli."
Boros I., Csordas-Toth E., Kiss A., Kiss I., Toeroek I., Udvardy A., Udvardy K., Venetianer P.
Biochim. Biophys. Acta 739:173-180(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The Escherichia coli mutant requiring D-glutamic acid is the result of mutations in two distinct genetic loci."
Dougherty T.J., Thanassi J.A., Pucci M.J.
J. Bacteriol. 175:111-116(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: B.
[4]"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[6]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]"Identification of the Escherichia coli murI gene, which is required for the biosynthesis of D-glutamic acid, a specific component of bacterial peptidoglycan."
Doublet P., van Heijenoort J., Mengin-Lecreulx D.
J. Bacteriol. 174:5772-5779(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: K12.
[8]"The murI gene of Escherichia coli is an essential gene that encodes a glutamate racemase activity."
Doublet P., van Heijenoort J., Bohin J.-P., Mengin-Lecreulx D.
J. Bacteriol. 175:2970-2979(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V00348 Genomic DNA. Translation: CAA23638.1. Different initiation.
V00347 Genomic DNA. Translation: CAA23637.1. Different initiation.
L14556 Genomic DNA. Translation: AAA23677.1. Different initiation.
U00006 Genomic DNA. Translation: AAC43073.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76949.2.
AP009048 Genomic DNA. Translation: BAE77344.1.
PIRI41187.
RefSeqNP_418402.2. NC_000913.2.
YP_491485.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2JFNX-ray1.90A1-285[»]
ProteinModelPortalP22634.
SMRP22634. Positions 20-285.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-10283N.
IntActP22634. 5 interactions.
MINTMINT-1222611.
STRING511145.b3967.

Proteomic databases

PaxDbP22634.
PRIDEP22634.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76949; AAC76949; b3967.
BAE77344; BAE77344; BAE77344.
GeneID12930612.
948467.
KEGGecj:Y75_p3221.
eco:b3967.
PATRIC32123455. VBIEscCol129921_4088.

Organism-specific databases

EchoBASEEB1189.
EcoGeneEG11204. murI.

Phylogenomic databases

eggNOGCOG0796.
HOGENOMHOG000262397.
KOK01776.
OMAVIMACNT.
ProtClustDBPRK00865.

Enzyme and pathway databases

BioCycEcoCyc:GLUTRACE-MONOMER.
ECOL316407:JW5550-MONOMER.
MetaCyc:GLUTRACE-MONOMER.
SABIO-RKP22634.
UniPathwayUPA00219.

Gene expression databases

GenevestigatorP22634.

Family and domain databases

Gene3D3.40.50.1860. 1 hit.
HAMAPMF_00258. Glu_racemase.
InterProIPR015942. Asp/Glu/hydantoin_racemase.
IPR001920. Asp/Glu_race.
IPR018187. Asp/Glu_racemase_AS.
IPR004391. Glu_race.
[Graphical view]
PANTHERPTHR21198. PTHR21198. 1 hit.
PfamPF01177. Asp_Glu_race. 1 hit.
[Graphical view]
SUPFAMSSF53681. Asp/Glu_race. 2 hits.
TIGRFAMsTIGR00067. glut_race. 1 hit.
PROSITEPS00923. ASP_GLU_RACEMASE_1. 1 hit.
PS00924. ASP_GLU_RACEMASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP22634.

Entry information

Entry nameMURI_ECOLI
AccessionPrimary (citable) accession number: P22634
Secondary accession number(s): P78133, Q2M8R2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1995
Last modified: May 1, 2013
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families