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Protein

Glutamate racemase

Gene

murI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Provides the (R)-glutamate required for cell wall biosynthesis.UniRule annotation1 Publication2 Publications

Catalytic activityi

L-glutamate = D-glutamate.UniRule annotation2 Publications

Enzyme regulationi

The low basal catalytic activity in increased 1000-fold in the presence of UDP-MurNAc-Ala, the product of the preceding enzyme in the peptidoglycan biosynthesis.1 Publication

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 Publication2 Publications
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei92Proton donor/acceptorUniRule annotationBy similarity1
Binding sitei104UDP-MurNAc-L-AlaCombined sources1 Publication1
Active sitei204Proton donor/acceptorUniRule annotationBy similarity1

GO - Molecular functioni

  • glutamate racemase activity Source: EcoCyc

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan biosynthetic process Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:GLUTRACE-MONOMER.
ECOL316407:JW5550-MONOMER.
MetaCyc:GLUTRACE-MONOMER.
SABIO-RKP22634.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate racemase1 PublicationUniRule annotation (EC:5.1.1.3UniRule annotation2 Publications)
Gene namesi
Name:murI2 PublicationsUniRule annotation
Synonyms:dga, glr, yijA
Ordered Locus Names:b3967, JW5550
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11204. murI.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000954701 – 285Glutamate racemaseAdd BLAST285

Proteomic databases

PaxDbiP22634.
PRIDEiP22634.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4259606. 249 interactors.
DIPiDIP-10283N.
IntActiP22634. 13 interactors.
MINTiMINT-1222611.
STRINGi511145.b3967.

Structurei

Secondary structure

1285
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi21 – 31Combined sources11
Helixi33 – 43Combined sources11
Beta strandi47 – 53Combined sources7
Turni55 – 57Combined sources3
Turni60 – 62Combined sources3
Helixi65 – 82Combined sources18
Beta strandi86 – 90Combined sources5
Helixi93 – 106Combined sources14
Helixi118 – 124Combined sources7
Beta strandi126 – 134Combined sources9
Helixi138 – 140Combined sources3
Helixi142 – 150Combined sources9
Beta strandi155 – 161Combined sources7
Helixi163 – 173Combined sources11
Helixi180 – 186Combined sources7
Helixi188 – 191Combined sources4
Beta strandi198 – 202Combined sources5
Helixi207 – 210Combined sources4
Helixi211 – 217Combined sources7
Beta strandi223 – 225Combined sources3
Helixi228 – 241Combined sources14
Beta strandi253 – 258Combined sources6
Helixi261 – 264Combined sources4
Helixi267 – 272Combined sources6
Beta strandi277 – 280Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JFNX-ray1.90A1-285[»]
ProteinModelPortaliP22634.
SMRiP22634.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP22634.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni28 – 29Substrate bindingUniRule annotationCombined sources1 Publication2
Regioni60 – 61Substrate bindingUniRule annotationCombined sources1 Publication2
Regioni93 – 94Substrate bindingUniRule annotationCombined sources1 Publication2
Regioni113 – 119UDP-MurNAc-Ala bindingCombined sources1 Publication7
Regioni205 – 206Substrate bindingUniRule annotationCombined sources1 Publication2

Sequence similaritiesi

Belongs to the aspartate/glutamate racemases family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F03. Bacteria.
COG0796. LUCA.
HOGENOMiHOG000262397.
InParanoidiP22634.
KOiK01776.
OMAiVPYGSKS.
PhylomeDBiP22634.

Family and domain databases

Gene3Di3.40.50.1860. 1 hit.
HAMAPiMF_00258. Glu_racemase. 1 hit.
InterProiIPR015942. Asp/Glu/hydantoin_racemase.
IPR001920. Asp/Glu_race.
IPR018187. Asp/Glu_racemase_AS_1.
IPR033134. Asp/Glu_racemase_AS_2.
IPR004391. Glu_race.
[Graphical view]
PfamiPF01177. Asp_Glu_race. 1 hit.
[Graphical view]
SUPFAMiSSF53681. SSF53681. 2 hits.
TIGRFAMsiTIGR00067. glut_race. 1 hit.
PROSITEiPS00923. ASP_GLU_RACEMASE_1. 1 hit.
PS00924. ASP_GLU_RACEMASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P22634-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATKLQDGNT PCLAATPSEP RPTVLVFDSG VGGLSVYDEI RHLLPDLHYI
60 70 80 90 100
YAFDNVAFPY GEKSEAFIVE RVVAIVTAVQ ERYPLALAVV ACNTASTVSL
110 120 130 140 150
PALREKFDFP VVGVVPAIKP AARLTANGIV GLLATRGTVK RSYTHELIAR
160 170 180 190 200
FANECQIEML GSAEMVELAE AKLHGEDVSL DALKRILRPW LRMKEPPDTV
210 220 230 240 250
VLGCTHFPLL QEELLQVLPE GTRLVDSGAA IARRTAWLLE HEAPDAKSAD
260 270 280
ANIAFCMAMT PGAEQLLPVL QRYGFETLEK LAVLG
Length:285
Mass (Da):31,002
Last modified:February 1, 1995 - v2
Checksum:iEC87281B577B8E93
GO

Sequence cautioni

The sequence AAA23677 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAC43073 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA23637 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA23638 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00348 Genomic DNA. Translation: CAA23638.1. Different initiation.
V00347 Genomic DNA. Translation: CAA23637.1. Different initiation.
L14556 Genomic DNA. Translation: AAA23677.1. Different initiation.
U00006 Genomic DNA. Translation: AAC43073.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76949.2.
AP009048 Genomic DNA. Translation: BAE77344.1.
PIRiI41187.
RefSeqiNP_418402.2. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC76949; AAC76949; b3967.
BAE77344; BAE77344; BAE77344.
GeneIDi948467.
KEGGiecj:JW5550.
eco:b3967.
PATRICi32123455. VBIEscCol129921_4088.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00348 Genomic DNA. Translation: CAA23638.1. Different initiation.
V00347 Genomic DNA. Translation: CAA23637.1. Different initiation.
L14556 Genomic DNA. Translation: AAA23677.1. Different initiation.
U00006 Genomic DNA. Translation: AAC43073.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76949.2.
AP009048 Genomic DNA. Translation: BAE77344.1.
PIRiI41187.
RefSeqiNP_418402.2. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JFNX-ray1.90A1-285[»]
ProteinModelPortaliP22634.
SMRiP22634.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259606. 249 interactors.
DIPiDIP-10283N.
IntActiP22634. 13 interactors.
MINTiMINT-1222611.
STRINGi511145.b3967.

Proteomic databases

PaxDbiP22634.
PRIDEiP22634.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76949; AAC76949; b3967.
BAE77344; BAE77344; BAE77344.
GeneIDi948467.
KEGGiecj:JW5550.
eco:b3967.
PATRICi32123455. VBIEscCol129921_4088.

Organism-specific databases

EchoBASEiEB1189.
EcoGeneiEG11204. murI.

Phylogenomic databases

eggNOGiENOG4105F03. Bacteria.
COG0796. LUCA.
HOGENOMiHOG000262397.
InParanoidiP22634.
KOiK01776.
OMAiVPYGSKS.
PhylomeDBiP22634.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciEcoCyc:GLUTRACE-MONOMER.
ECOL316407:JW5550-MONOMER.
MetaCyc:GLUTRACE-MONOMER.
SABIO-RKP22634.

Miscellaneous databases

EvolutionaryTraceiP22634.
PROiP22634.

Family and domain databases

Gene3Di3.40.50.1860. 1 hit.
HAMAPiMF_00258. Glu_racemase. 1 hit.
InterProiIPR015942. Asp/Glu/hydantoin_racemase.
IPR001920. Asp/Glu_race.
IPR018187. Asp/Glu_racemase_AS_1.
IPR033134. Asp/Glu_racemase_AS_2.
IPR004391. Glu_race.
[Graphical view]
PfamiPF01177. Asp_Glu_race. 1 hit.
[Graphical view]
SUPFAMiSSF53681. SSF53681. 2 hits.
TIGRFAMsiTIGR00067. glut_race. 1 hit.
PROSITEiPS00923. ASP_GLU_RACEMASE_1. 1 hit.
PS00924. ASP_GLU_RACEMASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMURI_ECOLI
AccessioniPrimary (citable) accession number: P22634
Secondary accession number(s): P78133, Q2M8R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.