P22630 (AMY1_AERHY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Alpha-amylase EC=3.2.1.1 Alternative name(s): 1,4-alpha-D-glucan glucanohydrolase
Organism	Aeromonas hydrophila
Taxonomic identifier	644 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 464	443	Alpha-amylase		PRO_0000001326
Regions
	Region	218 – 219	2	Substrate binding By similarity
Sites
	Active site	215	1	Nucleophile By similarity
	Active site	242	1	Proton donor By similarity
	Binding site	107	1	Substrate By similarity
	Binding site	213	1	Substrate By similarity
	Binding site	247	1	Substrate; via amide nitrogen By similarity
	Binding site	313	1	Substrate By similarity
	Site	314	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22630 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: E82A3298EEAC5C49	FASTA	464	51,656
	10 20 30 40 50 60 MKNTAGILAI AGMLIAPLAH ADVILHAFNW KYSEVTAKAD LIKGAGYKQV LISPPLKSSG 70 80 90 100 110 120 NEWWARYQPQ DLRLVDSPLG NKQDLEQLIA AMQARGIAVY ADVVLNHMAN ESWKRNDLNY 130 140 150 160 170 180 PGTELLGQYA ANPDYYSRQR LFGDLGQNLL SASDFHPEGC ITDWSDPGHV QYWRLCGGAG 190 200 210 220 230 240 DKGLPDLDPN NWVVSQQQAY LKALKGMGIK GFRVDAVKHM SDYQINAVFT PEIKQGMHVF 250 260 270 280 290 300 GEVITTGGAG STDYERFLKP YLDNSGQGAY DFPLFASLRG ALGYGGSMNQ LADPGAYGQA 310 320 330 340 350 360 LPGNRAVTFA ITHDIPTNDG FRYQILNQTD EKLAYAYLLG RDGGSPLVYS DHGETQDKDG 370 380 390 400 410 420 LRWQDYYLRS DLKGMIRFHN AVQGQPMQLI GSGDCFVLFK RGKQGLVGVN KCDYEQEYWL 430 440 450 460 DTAKFELNWY RNYKDVLDQS AVINVQSQWV RVAMPARRPP LAAE

References

[1]	"Molecular cloning, characterization, and nucleotide sequence of an extracellular amylase gene from Aeromonas hydrophila." Gobius K.S., Pemberton J.M. J. Bacteriol. 170:1325-1332(1988) [PubMed: 2449422] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JMP636.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M20401 Genomic DNA. Translation: AAA21936.1.
PIR	A28631.
3D structure databases
ProteinModelPortal	P22630.
ModBase	Search...
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006046. Glyco_hydro_13. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10357. Alpha_amylase. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
PRINTS	PR00110. ALPHAAMYLASE.
SMART	SM00642. Aamy. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AMY1_AERHY

Accession

Primary (citable) accession number: P22630

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program