ID   SAV_STRAV               Reviewed;         183 AA.
AC   P22629;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   27-MAR-2024, entry version 159.
DE   RecName: Full=Streptavidin;
DE   Flags: Precursor;
OS   Streptomyces avidinii.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=1895;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 25-64.
RX   PubMed=3951999; DOI=10.1093/nar/14.4.1871;
RA   Argarana C.E., Kuntz I.D., Birken S., Axel R., Cantor C.R.;
RT   "Molecular cloning and nucleotide sequence of the streptavidin gene.";
RL   Nucleic Acids Res. 14:1871-1882(1986).
RN   [2]
RP   IMPORTANCE OF TRP IN BIOTIN-BINDING.
RX   PubMed=3223904; DOI=10.1042/bj2560279;
RA   Gitlin G., Bayer E.A., Wilchek M.;
RT   "Studies on the biotin-binding site of streptavidin. Tryptophan residues
RT   involved in the active site.";
RL   Biochem. J. 256:279-282(1988).
RN   [3]
RP   IMPORTANCE OF TYR IN BIOTIN-BINDING.
RX   PubMed=2386489; DOI=10.1042/bj2690527;
RA   Gitlin G., Bayer E.A., Wilchek M.;
RT   "Studies on the biotin-binding sites of avidin and streptavidin. Tyrosine
RT   residues are involved in the binding site.";
RL   Biochem. J. 269:527-530(1990).
RN   [4]
RP   CELL-BINDING.
RX   PubMed=2390089; DOI=10.1016/0006-291x(90)90526-s;
RA   Alon R., Bayer E.A., Wilchek M.;
RT   "Streptavidin contains an RYD sequence which mimics the RGD receptor domain
RT   of fibronectin.";
RL   Biochem. Biophys. Res. Commun. 170:1236-1241(1990).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 37-157.
RX   PubMed=2911722; DOI=10.1126/science.2911722;
RA   Weber P.C., Ohlendorf D.H., Wendoloski J.J., Salemme F.R.;
RT   "Structural origins of high-affinity biotin binding to streptavidin.";
RL   Science 243:85-88(1989).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 40-157.
RX   PubMed=9194176; DOI=10.1002/pro.5560060604;
RA   Freitag S., le Trong I., Klumb L., Stayton P.S., Stenkamp R.E.;
RT   "Structural studies of the streptavidin binding loop.";
RL   Protein Sci. 6:1157-1166(1997).
RN   [7]
RP   X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 37-157.
RX   PubMed=9148939; DOI=10.1074/jbc.272.20.13220;
RA   Katz B.A., Cass R.T.;
RT   "In crystals of complexes of streptavidin with peptide ligands containing
RT   the HPQ sequence the pKa of the peptide histidine is less than 3.0.";
RL   J. Biol. Chem. 272:13220-13228(1997).
RN   [8]
RP   X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 37-157.
RX   PubMed=9405158; DOI=10.1006/jmbi.1997.1444;
RA   Katz B.A.;
RT   "Binding of biotin to streptavidin stabilizes intersubunit salt bridges
RT   between Asp61 and His87 at low pH.";
RL   J. Mol. Biol. 274:776-800(1997).
RN   [9]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 37-157 OF MUTANTS.
RX   PubMed=9636711; DOI=10.1006/jmbi.1998.1735;
RA   Freitag S., le Trong I., Chilkoti A., Klumb L.A., Stayton P.S.,
RA   Stenkamp R.E.;
RT   "Structural studies of binding site tryptophan mutants in the high-affinity
RT   streptavidin-biotin complex.";
RL   J. Mol. Biol. 279:211-221(1998).
CC   -!- FUNCTION: The biological function of streptavidin is not known. Forms a
CC       strong non-covalent specific complex with biotin (one molecule of
CC       biotin per subunit of streptavidin).
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the avidin/streptavidin family. {ECO:0000305}.
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DR   EMBL; X03591; CAA27265.1; -; Genomic_DNA.
DR   PIR; A23513; A23513.
DR   PDB; 1DF8; X-ray; 1.51 A; A/B=37-163.
DR   PDB; 1HQQ; X-ray; 1.70 A; A/B/C/D=36-163.
DR   PDB; 1HXL; X-ray; 1.80 A; A/B=36-163.
DR   PDB; 1HXZ; X-ray; 1.80 A; A/B=36-163.
DR   PDB; 1HY2; X-ray; 2.00 A; A/B/C/D=36-163.
DR   PDB; 1I9H; X-ray; 2.40 A; A/B=25-163.
DR   PDB; 1KFF; X-ray; 1.90 A; A/B/C/D=38-163.
DR   PDB; 1KL3; X-ray; 1.70 A; A/B/C/D=38-163.
DR   PDB; 1KL4; X-ray; 1.70 A; A/B/C/D=38-163.
DR   PDB; 1KL5; X-ray; 1.80 A; A/B/C/D=38-163.
DR   PDB; 1LCV; X-ray; 2.30 A; A/B=39-159.
DR   PDB; 1LCW; X-ray; 2.20 A; A/B=39-159.
DR   PDB; 1LCZ; X-ray; 1.95 A; A/B=25-159.
DR   PDB; 1LUQ; X-ray; 0.96 A; A/B=37-163.
DR   PDB; 1MEP; X-ray; 1.65 A; A/B/C/D=37-163.
DR   PDB; 1MK5; X-ray; 1.40 A; A/B=37-163.
DR   PDB; 1MM9; X-ray; 1.66 A; A=37-163.
DR   PDB; 1MOY; X-ray; 1.55 A; A=37-163.
DR   PDB; 1N43; X-ray; 1.89 A; A/B/C/D=37-163.
DR   PDB; 1N4J; X-ray; 2.18 A; A=37-163.
DR   PDB; 1N7Y; X-ray; 1.96 A; A/B/C/D=37-163.
DR   PDB; 1N9M; X-ray; 1.60 A; A/B/C/D=37-163.
DR   PDB; 1N9Y; X-ray; 1.53 A; A/B/C/D=37-163.
DR   PDB; 1NBX; X-ray; 1.70 A; A/B/C/D=37-163.
DR   PDB; 1NC9; X-ray; 1.80 A; A/B/C/D=37-163.
DR   PDB; 1NDJ; X-ray; 1.81 A; A/B/C/D=37-163.
DR   PDB; 1NQM; X-ray; 1.70 A; A/B/C/D=24-159.
DR   PDB; 1PTS; X-ray; 2.00 A; A/B=37-157.
DR   PDB; 1RST; X-ray; 1.70 A; B=38-163.
DR   PDB; 1RSU; X-ray; 1.70 A; B=38-163.
DR   PDB; 1RXH; X-ray; 2.90 A; A/B=37-163.
DR   PDB; 1RXJ; X-ray; 1.14 A; A/B/C/D=39-159.
DR   PDB; 1RXK; X-ray; 1.70 A; A/B=38-159.
DR   PDB; 1SLD; X-ray; 2.50 A; B=25-159.
DR   PDB; 1SLE; X-ray; 2.00 A; B/D=25-159.
DR   PDB; 1SLF; X-ray; 1.76 A; B/D=25-159.
DR   PDB; 1SLG; X-ray; 1.76 A; B/D=25-159.
DR   PDB; 1SRE; X-ray; 1.78 A; A/B=37-157.
DR   PDB; 1SRF; X-ray; 2.00 A; A/B=37-157.
DR   PDB; 1SRG; X-ray; 1.80 A; A/B=37-157.
DR   PDB; 1SRH; X-ray; 2.20 A; A/B=37-157.
DR   PDB; 1SRI; X-ray; 1.65 A; A/B=37-157.
DR   PDB; 1SRJ; X-ray; 1.80 A; A/B=37-157.
DR   PDB; 1STP; X-ray; 2.60 A; A=25-183.
DR   PDB; 1STR; X-ray; 1.80 A; B/D=37-159.
DR   PDB; 1STS; X-ray; 1.95 A; B/D=37-159.
DR   PDB; 1SWA; X-ray; 1.90 A; A/B/C/D=37-163.
DR   PDB; 1SWB; X-ray; 1.85 A; A/B/C/D=37-163.
DR   PDB; 1SWC; X-ray; 1.80 A; A/B/C/D=37-163.
DR   PDB; 1SWD; X-ray; 1.90 A; A/B/C/D=37-163.
DR   PDB; 1SWE; X-ray; 2.06 A; A/B/C/D=37-163.
DR   PDB; 1SWF; X-ray; 2.00 A; A/B/C/D=75-163.
DR   PDB; 1SWG; X-ray; 1.80 A; A/B/C/D=75-178.
DR   PDB; 1SWH; X-ray; 1.70 A; A/B/C/D=37-163.
DR   PDB; 1SWJ; X-ray; 2.00 A; A/B/C/D=37-163.
DR   PDB; 1SWK; X-ray; 2.00 A; A/B/C/D=37-163.
DR   PDB; 1SWL; X-ray; 1.80 A; A/B/C/D=37-163.
DR   PDB; 1SWN; X-ray; 2.20 A; A/B/C/D=37-163.
DR   PDB; 1SWO; X-ray; 1.95 A; A/B/C/D=37-163.
DR   PDB; 1SWP; X-ray; 2.00 A; A/B/C/D=37-163.
DR   PDB; 1SWQ; X-ray; 1.90 A; A/B/C/D=37-163.
DR   PDB; 1SWR; X-ray; 1.90 A; A/B/C/D=37-163.
DR   PDB; 1SWS; X-ray; 2.00 A; A/B/C/D=37-163.
DR   PDB; 1SWT; X-ray; 2.00 A; A/B=37-163.
DR   PDB; 1SWU; X-ray; 1.14 A; A/B/C/D=37-163.
DR   PDB; 1VWA; X-ray; 1.85 A; B/D=37-159.
DR   PDB; 1VWB; X-ray; 1.82 A; B=37-159.
DR   PDB; 1VWC; X-ray; 1.86 A; B=37-159.
DR   PDB; 1VWD; X-ray; 1.87 A; B=37-159.
DR   PDB; 1VWE; X-ray; 1.50 A; B=37-159.
DR   PDB; 1VWF; X-ray; 1.92 A; B=37-159.
DR   PDB; 1VWG; X-ray; 1.46 A; B=37-159.
DR   PDB; 1VWH; X-ray; 1.48 A; B=37-159.
DR   PDB; 1VWI; X-ray; 1.50 A; B/D=37-159.
DR   PDB; 1VWJ; X-ray; 1.45 A; B/D=37-159.
DR   PDB; 1VWK; X-ray; 1.45 A; B/D=37-159.
DR   PDB; 1VWL; X-ray; 1.45 A; B/D=37-159.
DR   PDB; 1VWM; X-ray; 1.60 A; B=37-159.
DR   PDB; 1VWN; X-ray; 1.85 A; B=37-159.
DR   PDB; 1VWO; X-ray; 1.65 A; B=37-159.
DR   PDB; 1VWP; X-ray; 1.75 A; B=37-159.
DR   PDB; 1VWQ; X-ray; 1.70 A; B=37-159.
DR   PDB; 1VWR; X-ray; 1.50 A; B=37-159.
DR   PDB; 2BC3; X-ray; 1.54 A; A/B=38-183.
DR   PDB; 2F01; X-ray; 0.85 A; A/B=37-163.
DR   PDB; 2G5L; X-ray; 1.15 A; A/B=37-163.
DR   PDB; 2GH7; X-ray; 1.00 A; A/B=37-163.
DR   PDB; 2IZA; X-ray; 1.46 A; A=37-157.
DR   PDB; 2IZB; X-ray; 1.20 A; A=37-158.
DR   PDB; 2IZC; X-ray; 1.40 A; B/D=37-159.
DR   PDB; 2IZD; X-ray; 1.60 A; B/D=37-159.
DR   PDB; 2IZE; X-ray; 1.57 A; B/D=37-159.
DR   PDB; 2IZF; X-ray; 1.58 A; B/D=37-159.
DR   PDB; 2IZG; X-ray; 1.36 A; B/D=37-159.
DR   PDB; 2IZH; X-ray; 1.36 A; B/D=37-159.
DR   PDB; 2IZI; X-ray; 1.50 A; A=37-159.
DR   PDB; 2IZJ; X-ray; 1.30 A; A=37-159.
DR   PDB; 2IZK; X-ray; 1.30 A; A=37-159.
DR   PDB; 2IZL; X-ray; 1.48 A; B/D=37-159.
DR   PDB; 2QCB; X-ray; 1.65 A; A=38-183.
DR   PDB; 2RTA; X-ray; 1.39 A; A=25-159.
DR   PDB; 2RTB; X-ray; 1.50 A; B/D=25-159.
DR   PDB; 2RTC; X-ray; 1.50 A; B/D=25-159.
DR   PDB; 2RTD; X-ray; 1.65 A; B/D=25-159.
DR   PDB; 2RTE; X-ray; 1.50 A; B/D=25-159.
DR   PDB; 2RTF; X-ray; 1.47 A; B/D=25-159.
DR   PDB; 2RTG; X-ray; 1.39 A; B/D=25-159.
DR   PDB; 2RTH; X-ray; 1.56 A; B/D=25-159.
DR   PDB; 2RTI; X-ray; 1.40 A; B/D=25-159.
DR   PDB; 2RTJ; X-ray; 1.40 A; A=25-159.
DR   PDB; 2RTK; X-ray; 1.82 A; A=25-159.
DR   PDB; 2RTL; X-ray; 1.41 A; A=25-159.
DR   PDB; 2RTM; X-ray; 1.30 A; A=25-159.
DR   PDB; 2RTN; X-ray; 1.80 A; B/D=25-159.
DR   PDB; 2RTO; X-ray; 1.58 A; B/D=25-159.
DR   PDB; 2RTP; X-ray; 1.50 A; B/D=25-159.
DR   PDB; 2RTQ; X-ray; 1.39 A; B/D=25-159.
DR   PDB; 2RTR; X-ray; 1.62 A; B/D=25-159.
DR   PDB; 2WPU; X-ray; 1.92 A; A=38-183.
DR   PDB; 2Y3E; X-ray; 1.45 A; A/B=37-163.
DR   PDB; 2Y3F; X-ray; 1.49 A; A=37-163.
DR   PDB; 3MG5; X-ray; 1.30 A; A/B/C/D=37-163.
DR   PDB; 3PK2; X-ray; 1.90 A; A=38-183.
DR   PDB; 3RDM; X-ray; 1.60 A; A=37-164.
DR   PDB; 3RDO; X-ray; 1.40 A; A=37-164.
DR   PDB; 3RDQ; X-ray; 1.60 A; A=37-164.
DR   PDB; 3RDS; X-ray; 1.50 A; A=37-164.
DR   PDB; 3RDU; X-ray; 1.50 A; A=37-164.
DR   PDB; 3RDX; X-ray; 2.10 A; A/B=37-164.
DR   PDB; 3RE5; X-ray; 1.95 A; A=37-164.
DR   PDB; 3RE6; X-ray; 1.82 A; A=37-164.
DR   PDB; 3RY1; X-ray; 1.03 A; A/B/C/D=37-163.
DR   PDB; 3RY2; X-ray; 0.95 A; A/B=37-163.
DR   PDB; 3T6F; X-ray; 1.22 A; A/B=37-163.
DR   PDB; 3T6L; X-ray; 1.30 A; A=37-163.
DR   PDB; 3WYP; X-ray; 1.30 A; A/B/C/D=37-163.
DR   PDB; 3WYQ; X-ray; 1.00 A; A/B=37-163.
DR   PDB; 3WZN; X-ray; 1.30 A; A/B=37-163.
DR   PDB; 3WZO; X-ray; 1.50 A; A/B/C/D=37-163.
DR   PDB; 3WZP; X-ray; 1.20 A; A/B/C/D=37-163.
DR   PDB; 3WZQ; X-ray; 1.70 A; A/B/C/D=37-163.
DR   PDB; 3X00; X-ray; 1.30 A; A/B/C/D=37-163.
DR   PDB; 4BX5; X-ray; 1.43 A; A/C=37-72, B/D=37-163.
DR   PDB; 4BX6; X-ray; 1.60 A; A/B/C/D=37-163.
DR   PDB; 4BX7; X-ray; 2.26 A; A/B=37-163.
DR   PDB; 4CPE; X-ray; 1.06 A; A/B=37-163.
DR   PDB; 4CPF; X-ray; 1.14 A; A/B=37-163.
DR   PDB; 4CPH; X-ray; 1.64 A; A/B/C/D=37-163.
DR   PDB; 4CPI; X-ray; 1.54 A; A/B/C/D=37-163.
DR   PDB; 4DNE; X-ray; 1.88 A; A/B=1-183.
DR   PDB; 4EKV; X-ray; 2.00 A; A=25-183.
DR   PDB; 4GD9; X-ray; 1.50 A; A/B/C/D=37-163.
DR   PDB; 4GDA; X-ray; 1.00 A; A/B=37-163.
DR   PDB; 4GJS; X-ray; 1.85 A; A/B=38-183.
DR   PDB; 4GJV; X-ray; 2.40 A; A=38-183.
DR   PDB; 4IRW; X-ray; 1.40 A; A=36-163.
DR   PDB; 4JO6; X-ray; 1.75 A; A/B/C/D=25-183.
DR   PDB; 4OKA; X-ray; 2.50 A; A=38-183.
DR   PDB; 4Y59; X-ray; 1.22 A; A/B/C/D=39-159.
DR   PDB; 4Y5D; X-ray; 1.20 A; A/B/C/D=39-160.
DR   PDB; 4YVB; X-ray; 1.35 A; A/B/C/D=40-159.
DR   PDB; 5B5F; X-ray; 1.20 A; A/B/C/D=40-159.
DR   PDB; 5B5G; X-ray; 1.50 A; A/B/C/D=40-159.
DR   PDB; 5CSE; X-ray; 1.79 A; A/B=38-158.
DR   PDB; 5F2B; X-ray; 1.70 A; A=38-183.
DR   PDB; 5JD2; X-ray; 1.90 A; A/B/C/D=40-159.
DR   PDB; 5K67; X-ray; 1.70 A; A=38-183.
DR   PDB; 5K68; X-ray; 1.40 A; A=38-183.
DR   PDB; 5L3Y; X-ray; 1.70 A; A=38-183.
DR   PDB; 5N7X; X-ray; 1.12 A; A/B/E/F/H/K/M/O=1-183.
DR   PDB; 5N89; X-ray; 1.27 A; A/B/D/F/H/K/M/O=1-183.
DR   PDB; 5N8B; X-ray; 1.03 A; A/B/D/G=1-183.
DR   PDB; 5N8E; X-ray; 1.10 A; A/B/C/D=1-183.
DR   PDB; 5N8J; X-ray; 1.05 A; A/B/C/D=1-183.
DR   PDB; 5N8T; X-ray; 1.61 A; A=1-183.
DR   PDB; 5N8W; X-ray; 1.10 A; A/B=1-183.
DR   PDB; 5N99; X-ray; 1.50 A; A/B/D/G/I/K/M/O/Q/S/U/Y=1-183.
DR   PDB; 5TO2; X-ray; 1.65 A; A/B/C=39-163, D=39-162.
DR   PDB; 5VCQ; X-ray; 2.05 A; A/B/C/D=1-183.
DR   PDB; 5VKX; X-ray; 1.37 A; A=38-183.
DR   PDB; 5VL5; X-ray; 1.46 A; A=38-183.
DR   PDB; 5VL8; X-ray; 1.70 A; A=38-183.
DR   PDB; 5WBA; X-ray; 1.50 A; A=38-183.
DR   PDB; 5WBB; X-ray; 1.50 A; A=38-183.
DR   PDB; 5WBC; X-ray; 1.72 A; A=38-183.
DR   PDB; 5WBD; X-ray; 1.50 A; A=38-183.
DR   PDB; 6ANX; X-ray; 1.62 A; A=38-183.
DR   PDB; 6AUC; X-ray; 1.46 A; A=38-183.
DR   PDB; 6AUE; X-ray; 1.36 A; A=38-183.
DR   PDB; 6AUH; X-ray; 1.60 A; A=38-183.
DR   PDB; 6AUL; X-ray; 1.36 A; A=38-183.
DR   PDB; 6AUO; X-ray; 1.70 A; A=38-183.
DR   PDB; 6AVK; X-ray; 1.40 A; A/B=37-159.
DR   PDB; 6ESS; X-ray; 1.91 A; A=38-183.
DR   PDB; 6ESU; X-ray; 1.78 A; A=38-183.
DR   PDB; 6FH8; X-ray; 1.64 A; A=38-183.
DR   PDB; 6FRY; X-ray; 1.70 A; A=38-183.
DR   PDB; 6GH7; X-ray; 1.08 A; A/B/C/D=37-163.
DR   PDB; 6J6J; EM; 3.20 A; A/B/C/D=40-158.
DR   PDB; 6J6K; EM; 3.30 A; A/B/C/D=40-158.
DR   PDB; 6LNG; X-ray; 1.80 A; A/B/C/D/E/F=39-163.
DR   PDB; 6M9B; X-ray; 1.55 A; A/B/C/D=37-163.
DR   PDB; 6QBB; X-ray; 1.52 A; A/B=38-163.
DR   PDB; 6QSY; X-ray; 1.70 A; A=38-163.
DR   PDB; 6QW4; X-ray; 2.10 A; A=38-163.
DR   PDB; 6S4Q; X-ray; 1.85 A; A/B=39-183.
DR   PDB; 6S50; X-ray; 2.00 A; A/B=39-183.
DR   PDB; 6SOK; X-ray; 1.96 A; A/B/C/D=38-163.
DR   PDB; 6SOS; X-ray; 2.20 A; A/B/C/D=38-163.
DR   PDB; 6T1E; X-ray; 1.30 A; A=38-183.
DR   PDB; 6T1G; X-ray; 1.90 A; A/B=38-183.
DR   PDB; 6T1K; X-ray; 1.20 A; A=38-183.
DR   PDB; 6T2L; X-ray; 1.00 A; A=38-183.
DR   PDB; 6T2Y; X-ray; 1.80 A; A/B=38-183.
DR   PDB; 6T2Z; X-ray; 1.35 A; A/B=38-183.
DR   PDB; 6T30; X-ray; 1.80 A; A/B=38-183.
DR   PDB; 6T31; X-ray; 1.35 A; A/B=38-183.
DR   PDB; 6T32; X-ray; 1.75 A; A/B=38-183.
DR   PDB; 6TIP; X-ray; 2.10 A; A/B=38-163.
DR   PDB; 6UC3; X-ray; 1.84 A; A/B=37-163.
DR   PDB; 6UD1; X-ray; 1.55 A; A/B=37-163.
DR   PDB; 6UD6; X-ray; 1.50 A; A/B=37-163.
DR   PDB; 6UDB; X-ray; 1.55 A; A/B/C/D=37-163.
DR   PDB; 6UDC; X-ray; 2.10 A; A/B/C/D=37-163.
DR   PDB; 6UI0; X-ray; 1.40 A; A=38-183.
DR   PDB; 6UIU; X-ray; 1.35 A; A=38-183.
DR   PDB; 6UIY; X-ray; 1.47 A; A=38-183.
DR   PDB; 6UIZ; X-ray; 1.85 A; A=38-183.
DR   PDB; 6US6; X-ray; 1.50 A; A=38-183.
DR   PDB; 6VJK; X-ray; 1.60 A; A/B/C/D/E/F/G/H/I/J/K/L=38-160.
DR   PDB; 6VJL; X-ray; 1.30 A; A=25-183.
DR   PDB; 6VO9; X-ray; 1.50 A; A=38-183.
DR   PDB; 6VOB; X-ray; 1.70 A; A=38-183.
DR   PDB; 6VOZ; X-ray; 1.30 A; A=38-183.
DR   PDB; 6VP1; X-ray; 1.45 A; A/B/C/D=38-183.
DR   PDB; 6VP2; X-ray; 1.80 A; A/B/C/D=38-183.
DR   PDB; 6VP3; X-ray; 1.65 A; A/B/C/D=38-183.
DR   PDB; 6Y25; X-ray; 1.95 A; AAA=39-183.
DR   PDB; 6Y2M; X-ray; 1.95 A; AAA=39-183.
DR   PDB; 6Y2T; X-ray; 1.55 A; AAA=39-183.
DR   PDB; 6Y33; X-ray; 1.49 A; AAA=39-183.
DR   PDB; 6Y34; X-ray; 1.31 A; AAA=39-183.
DR   PDB; 6Y3Q; X-ray; 1.95 A; AAA=39-183.
DR   PDB; 7ALX; X-ray; 1.80 A; AAA/BBB=39-183.
DR   PDB; 7B74; X-ray; 1.85 A; AAA/BBB/CCC/DDD=39-183.
DR   PDB; 7DY0; EM; 1.92 A; A=1-183.
DR   PDB; 7EFC; EM; 1.70 A; A=1-183.
DR   PDB; 7EFD; EM; 1.77 A; A=1-183.
DR   PDB; 7EK8; X-ray; 1.70 A; A/B/C/D=38-160.
DR   PDB; 7EK9; X-ray; 1.10 A; A/B/C/D=38-160.
DR   PDB; 7KBY; X-ray; 1.70 A; A=38-183.
DR   PDB; 7KBZ; X-ray; 1.90 A; A/B/C/D=38-183.
DR   PDB; 7KNL; X-ray; 1.35 A; A=38-183.
DR   PDB; 7NLV; X-ray; 1.29 A; AAA/BBB/CCC/DDD=37-163.
DR   PDB; 7ZOF; X-ray; 1.74 A; A/B/C/D=38-183.
DR   PDB; 7ZX9; X-ray; 1.55 A; A=39-183.
DR   PDB; 7ZXZ; X-ray; 1.45 A; A/B=39-183.
DR   PDB; 8AQD; X-ray; 1.45 A; A=38-183.
DR   PDB; 8AQJ; X-ray; 1.85 A; A=38-183.
DR   PDB; 8AQO; X-ray; 1.90 A; A/B/C/D=38-183.
DR   PDB; 8AQX; X-ray; 1.85 A; A/B/C/D=38-183.
DR   PDB; 8AQY; X-ray; 1.65 A; A/B/C/D=38-183.
DR   PDB; 8CRN; X-ray; 2.00 A; A/B/C/D=39-183.
DR   PDB; 8CRP; X-ray; 2.00 A; A/B/C/D=39-183.
DR   PDB; 8GVK; EM; 2.20 A; A/B/C/D=1-183.
DR   PDB; 8P5Y; X-ray; 1.88 A; A/B/C/D=39-183.
DR   PDB; 8P5Z; X-ray; 1.56 A; A/B/C/D=38-183.
DR   PDB; 8PXG; X-ray; 1.80 A; A=38-163.
DR   PDBsum; 1DF8; -.
DR   PDBsum; 1HQQ; -.
DR   PDBsum; 1HXL; -.
DR   PDBsum; 1HXZ; -.
DR   PDBsum; 1HY2; -.
DR   PDBsum; 1I9H; -.
DR   PDBsum; 1KFF; -.
DR   PDBsum; 1KL3; -.
DR   PDBsum; 1KL4; -.
DR   PDBsum; 1KL5; -.
DR   PDBsum; 1LCV; -.
DR   PDBsum; 1LCW; -.
DR   PDBsum; 1LCZ; -.
DR   PDBsum; 1LUQ; -.
DR   PDBsum; 1MEP; -.
DR   PDBsum; 1MK5; -.
DR   PDBsum; 1MM9; -.
DR   PDBsum; 1MOY; -.
DR   PDBsum; 1N43; -.
DR   PDBsum; 1N4J; -.
DR   PDBsum; 1N7Y; -.
DR   PDBsum; 1N9M; -.
DR   PDBsum; 1N9Y; -.
DR   PDBsum; 1NBX; -.
DR   PDBsum; 1NC9; -.
DR   PDBsum; 1NDJ; -.
DR   PDBsum; 1NQM; -.
DR   PDBsum; 1PTS; -.
DR   PDBsum; 1RST; -.
DR   PDBsum; 1RSU; -.
DR   PDBsum; 1RXH; -.
DR   PDBsum; 1RXJ; -.
DR   PDBsum; 1RXK; -.
DR   PDBsum; 1SLD; -.
DR   PDBsum; 1SLE; -.
DR   PDBsum; 1SLF; -.
DR   PDBsum; 1SLG; -.
DR   PDBsum; 1SRE; -.
DR   PDBsum; 1SRF; -.
DR   PDBsum; 1SRG; -.
DR   PDBsum; 1SRH; -.
DR   PDBsum; 1SRI; -.
DR   PDBsum; 1SRJ; -.
DR   PDBsum; 1STP; -.
DR   PDBsum; 1STR; -.
DR   PDBsum; 1STS; -.
DR   PDBsum; 1SWA; -.
DR   PDBsum; 1SWB; -.
DR   PDBsum; 1SWC; -.
DR   PDBsum; 1SWD; -.
DR   PDBsum; 1SWE; -.
DR   PDBsum; 1SWF; -.
DR   PDBsum; 1SWG; -.
DR   PDBsum; 1SWH; -.
DR   PDBsum; 1SWJ; -.
DR   PDBsum; 1SWK; -.
DR   PDBsum; 1SWL; -.
DR   PDBsum; 1SWN; -.
DR   PDBsum; 1SWO; -.
DR   PDBsum; 1SWP; -.
DR   PDBsum; 1SWQ; -.
DR   PDBsum; 1SWR; -.
DR   PDBsum; 1SWS; -.
DR   PDBsum; 1SWT; -.
DR   PDBsum; 1SWU; -.
DR   PDBsum; 1VWA; -.
DR   PDBsum; 1VWB; -.
DR   PDBsum; 1VWC; -.
DR   PDBsum; 1VWD; -.
DR   PDBsum; 1VWE; -.
DR   PDBsum; 1VWF; -.
DR   PDBsum; 1VWG; -.
DR   PDBsum; 1VWH; -.
DR   PDBsum; 1VWI; -.
DR   PDBsum; 1VWJ; -.
DR   PDBsum; 1VWK; -.
DR   PDBsum; 1VWL; -.
DR   PDBsum; 1VWM; -.
DR   PDBsum; 1VWN; -.
DR   PDBsum; 1VWO; -.
DR   PDBsum; 1VWP; -.
DR   PDBsum; 1VWQ; -.
DR   PDBsum; 1VWR; -.
DR   PDBsum; 2BC3; -.
DR   PDBsum; 2F01; -.
DR   PDBsum; 2G5L; -.
DR   PDBsum; 2GH7; -.
DR   PDBsum; 2IZA; -.
DR   PDBsum; 2IZB; -.
DR   PDBsum; 2IZC; -.
DR   PDBsum; 2IZD; -.
DR   PDBsum; 2IZE; -.
DR   PDBsum; 2IZF; -.
DR   PDBsum; 2IZG; -.
DR   PDBsum; 2IZH; -.
DR   PDBsum; 2IZI; -.
DR   PDBsum; 2IZJ; -.
DR   PDBsum; 2IZK; -.
DR   PDBsum; 2IZL; -.
DR   PDBsum; 2QCB; -.
DR   PDBsum; 2RTA; -.
DR   PDBsum; 2RTB; -.
DR   PDBsum; 2RTC; -.
DR   PDBsum; 2RTD; -.
DR   PDBsum; 2RTE; -.
DR   PDBsum; 2RTF; -.
DR   PDBsum; 2RTG; -.
DR   PDBsum; 2RTH; -.
DR   PDBsum; 2RTI; -.
DR   PDBsum; 2RTJ; -.
DR   PDBsum; 2RTK; -.
DR   PDBsum; 2RTL; -.
DR   PDBsum; 2RTM; -.
DR   PDBsum; 2RTN; -.
DR   PDBsum; 2RTO; -.
DR   PDBsum; 2RTP; -.
DR   PDBsum; 2RTQ; -.
DR   PDBsum; 2RTR; -.
DR   PDBsum; 2WPU; -.
DR   PDBsum; 2Y3E; -.
DR   PDBsum; 2Y3F; -.
DR   PDBsum; 3MG5; -.
DR   PDBsum; 3PK2; -.
DR   PDBsum; 3RDM; -.
DR   PDBsum; 3RDO; -.
DR   PDBsum; 3RDQ; -.
DR   PDBsum; 3RDS; -.
DR   PDBsum; 3RDU; -.
DR   PDBsum; 3RDX; -.
DR   PDBsum; 3RE5; -.
DR   PDBsum; 3RE6; -.
DR   PDBsum; 3RY1; -.
DR   PDBsum; 3RY2; -.
DR   PDBsum; 3T6F; -.
DR   PDBsum; 3T6L; -.
DR   PDBsum; 3WYP; -.
DR   PDBsum; 3WYQ; -.
DR   PDBsum; 3WZN; -.
DR   PDBsum; 3WZO; -.
DR   PDBsum; 3WZP; -.
DR   PDBsum; 3WZQ; -.
DR   PDBsum; 3X00; -.
DR   PDBsum; 4BX5; -.
DR   PDBsum; 4BX6; -.
DR   PDBsum; 4BX7; -.
DR   PDBsum; 4CPE; -.
DR   PDBsum; 4CPF; -.
DR   PDBsum; 4CPH; -.
DR   PDBsum; 4CPI; -.
DR   PDBsum; 4DNE; -.
DR   PDBsum; 4EKV; -.
DR   PDBsum; 4GD9; -.
DR   PDBsum; 4GDA; -.
DR   PDBsum; 4GJS; -.
DR   PDBsum; 4GJV; -.
DR   PDBsum; 4IRW; -.
DR   PDBsum; 4JO6; -.
DR   PDBsum; 4OKA; -.
DR   PDBsum; 4Y59; -.
DR   PDBsum; 4Y5D; -.
DR   PDBsum; 4YVB; -.
DR   PDBsum; 5B5F; -.
DR   PDBsum; 5B5G; -.
DR   PDBsum; 5CSE; -.
DR   PDBsum; 5F2B; -.
DR   PDBsum; 5JD2; -.
DR   PDBsum; 5K67; -.
DR   PDBsum; 5K68; -.
DR   PDBsum; 5L3Y; -.
DR   PDBsum; 5N7X; -.
DR   PDBsum; 5N89; -.
DR   PDBsum; 5N8B; -.
DR   PDBsum; 5N8E; -.
DR   PDBsum; 5N8J; -.
DR   PDBsum; 5N8T; -.
DR   PDBsum; 5N8W; -.
DR   PDBsum; 5N99; -.
DR   PDBsum; 5TO2; -.
DR   PDBsum; 5VCQ; -.
DR   PDBsum; 5VKX; -.
DR   PDBsum; 5VL5; -.
DR   PDBsum; 5VL8; -.
DR   PDBsum; 5WBA; -.
DR   PDBsum; 5WBB; -.
DR   PDBsum; 5WBC; -.
DR   PDBsum; 5WBD; -.
DR   PDBsum; 6ANX; -.
DR   PDBsum; 6AUC; -.
DR   PDBsum; 6AUE; -.
DR   PDBsum; 6AUH; -.
DR   PDBsum; 6AUL; -.
DR   PDBsum; 6AUO; -.
DR   PDBsum; 6AVK; -.
DR   PDBsum; 6ESS; -.
DR   PDBsum; 6ESU; -.
DR   PDBsum; 6FH8; -.
DR   PDBsum; 6FRY; -.
DR   PDBsum; 6GH7; -.
DR   PDBsum; 6J6J; -.
DR   PDBsum; 6J6K; -.
DR   PDBsum; 6LNG; -.
DR   PDBsum; 6M9B; -.
DR   PDBsum; 6QBB; -.
DR   PDBsum; 6QSY; -.
DR   PDBsum; 6QW4; -.
DR   PDBsum; 6S4Q; -.
DR   PDBsum; 6S50; -.
DR   PDBsum; 6SOK; -.
DR   PDBsum; 6SOS; -.
DR   PDBsum; 6T1E; -.
DR   PDBsum; 6T1G; -.
DR   PDBsum; 6T1K; -.
DR   PDBsum; 6T2L; -.
DR   PDBsum; 6T2Y; -.
DR   PDBsum; 6T2Z; -.
DR   PDBsum; 6T30; -.
DR   PDBsum; 6T31; -.
DR   PDBsum; 6T32; -.
DR   PDBsum; 6TIP; -.
DR   PDBsum; 6UC3; -.
DR   PDBsum; 6UD1; -.
DR   PDBsum; 6UD6; -.
DR   PDBsum; 6UDB; -.
DR   PDBsum; 6UDC; -.
DR   PDBsum; 6UI0; -.
DR   PDBsum; 6UIU; -.
DR   PDBsum; 6UIY; -.
DR   PDBsum; 6UIZ; -.
DR   PDBsum; 6US6; -.
DR   PDBsum; 6VJK; -.
DR   PDBsum; 6VJL; -.
DR   PDBsum; 6VO9; -.
DR   PDBsum; 6VOB; -.
DR   PDBsum; 6VOZ; -.
DR   PDBsum; 6VP1; -.
DR   PDBsum; 6VP2; -.
DR   PDBsum; 6VP3; -.
DR   PDBsum; 6Y25; -.
DR   PDBsum; 6Y2M; -.
DR   PDBsum; 6Y2T; -.
DR   PDBsum; 6Y33; -.
DR   PDBsum; 6Y34; -.
DR   PDBsum; 6Y3Q; -.
DR   PDBsum; 7ALX; -.
DR   PDBsum; 7B74; -.
DR   PDBsum; 7DY0; -.
DR   PDBsum; 7EFC; -.
DR   PDBsum; 7EFD; -.
DR   PDBsum; 7EK8; -.
DR   PDBsum; 7EK9; -.
DR   PDBsum; 7KBY; -.
DR   PDBsum; 7KBZ; -.
DR   PDBsum; 7KNL; -.
DR   PDBsum; 7NLV; -.
DR   PDBsum; 7ZOF; -.
DR   PDBsum; 7ZX9; -.
DR   PDBsum; 7ZXZ; -.
DR   PDBsum; 8AQD; -.
DR   PDBsum; 8AQJ; -.
DR   PDBsum; 8AQO; -.
DR   PDBsum; 8AQX; -.
DR   PDBsum; 8AQY; -.
DR   PDBsum; 8CRN; -.
DR   PDBsum; 8CRP; -.
DR   PDBsum; 8GVK; -.
DR   PDBsum; 8P5Y; -.
DR   PDBsum; 8P5Z; -.
DR   PDBsum; 8PXG; -.
DR   AlphaFoldDB; P22629; -.
DR   EMDB; EMD-0689; -.
DR   EMDB; EMD-0690; -.
DR   EMDB; EMD-3090; -.
DR   EMDB; EMD-3091; -.
DR   EMDB; EMD-30913; -.
DR   EMDB; EMD-31083; -.
DR   EMDB; EMD-31084; -.
DR   PCDDB; P22629; -.
DR   SMR; P22629; -.
DR   BindingDB; P22629; -.
DR   ChEMBL; CHEMBL1075026; -.
DR   DrugBank; DB08196; 2-((3',5'-DIMETHOXY-4'-HYDROXYPHENYL)AZO)BENZOIC ACID.
DR   DrugBank; DB07667; 2-((3',5'-DIMETHYL-4'-HYDROXYPHENYL)AZO)BENZOIC ACID.
DR   DrugBank; DB08181; 2-((3'-METHYL-4'-HYDROXYPHENYL)AZO)BENZOIC ACID.
DR   DrugBank; DB08216; 2-((3'-TERTBUTYL-4'-HYDROXYPHENYL)AZO)BENZOIC ACID.
DR   DrugBank; DB08252; 2-((4'-HYDROXYNAPHTHYL)-AZO)BENZOIC ACID.
DR   DrugBank; DB07880; 2-((4'-HYDROXYPHENYL)-AZO)BENZOIC ACID.
DR   DrugBank; DB03353; 2-Iminobiotin.
DR   DrugBank; DB02674; 4-(2-Oxo-Hexahydro-Thieno[3,4-D]Imidazol-4-Yl)-Butyricacid.
DR   DrugBank; DB04650; 5-[(3AS,4R,6AR)-2-OXOHEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL]PENTANOIC ACID.
DR   DrugBank; DB03112; 6-(2-Oxo-Hexahydro-Thieno[3,4-D]Imidazol-4-Yl)-Hexanoic Acid.
DR   DrugBank; DB03139; 6-[5-(2-Oxo-Hexahydro-Thieno[3,4-D]Imidazol-4-Yl)-Pentanoylamino]-Hexanoic Acid.
DR   DrugBank; DB03533; Acetyleneurea.
DR   DrugBank; DB03549; Biotinyl P-Nitroaniline.
DR   DrugBank; DB01942; Formic acid.
DR   DrugBank; DB04464; N-Formylmethionine.
DR   SABIO-RK; P22629; -.
DR   EvolutionaryTrace; P22629; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0009374; F:biotin binding; IEA:InterPro.
DR   Gene3D; 2.40.128.30; Avidin-like; 1.
DR   InterPro; IPR005469; Avidin.
DR   InterPro; IPR017889; Avidin-like_CS.
DR   InterPro; IPR036896; Avidin-like_sf.
DR   InterPro; IPR005468; Avidin/str.
DR   PANTHER; PTHR34399:SF3; AVIDIN; 1.
DR   PANTHER; PTHR34399; AVIDIN-RELATED; 1.
DR   Pfam; PF01382; Avidin; 1.
DR   PRINTS; PR00709; AVIDIN.
DR   SUPFAM; SSF50876; Avidin/streptavidin; 1.
DR   PROSITE; PS00577; AVIDIN_1; 1.
DR   PROSITE; PS51326; AVIDIN_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Biotin; Direct protein sequencing; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000269|PubMed:3951999"
FT   CHAIN           25..183
FT                   /note="Streptavidin"
FT                   /id="PRO_0000002729"
FT   DOMAIN          37..159
FT                   /note="Avidin-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00656"
FT   MOTIF           83..85
FT                   /note="Cell attachment site; atypical"
FT   BINDING         67
FT                   /ligand="biotin"
FT                   /ligand_id="ChEBI:CHEBI:57586"
FT   BINDING         78
FT                   /ligand="biotin"
FT                   /ligand_id="ChEBI:CHEBI:57586"
FT   BINDING         116
FT                   /ligand="biotin"
FT                   /ligand_id="ChEBI:CHEBI:57586"
FT   BINDING         132
FT                   /ligand="biotin"
FT                   /ligand_id="ChEBI:CHEBI:57586"
FT   BINDING         144
FT                   /ligand="biotin"
FT                   /ligand_id="ChEBI:CHEBI:57586"
FT   TURN            39..41
FT                   /evidence="ECO:0007829|PDB:2F01"
FT   STRAND          43..47
FT                   /evidence="ECO:0007829|PDB:2F01"
FT   STRAND          48..50
FT                   /evidence="ECO:0007829|PDB:5N8W"
FT   STRAND          52..57
FT                   /evidence="ECO:0007829|PDB:2F01"
FT   STRAND          61..68
FT                   /evidence="ECO:0007829|PDB:2F01"
FT   STRAND          70..73
FT                   /evidence="ECO:0007829|PDB:2F01"
FT   HELIX           74..76
FT                   /evidence="ECO:0007829|PDB:5N8B"
FT   STRAND          78..84
FT                   /evidence="ECO:0007829|PDB:2F01"
FT   STRAND          90..92
FT                   /evidence="ECO:0007829|PDB:2F01"
FT   STRAND          95..104
FT                   /evidence="ECO:0007829|PDB:2F01"
FT   STRAND          109..121
FT                   /evidence="ECO:0007829|PDB:2F01"
FT   STRAND          123..125
FT                   /evidence="ECO:0007829|PDB:3RY1"
FT   STRAND          127..136
FT                   /evidence="ECO:0007829|PDB:2F01"
FT   HELIX           140..145
FT                   /evidence="ECO:0007829|PDB:2F01"
FT   STRAND          147..156
FT                   /evidence="ECO:0007829|PDB:2F01"
FT   HELIX           161..163
FT                   /evidence="ECO:0007829|PDB:1SWF"
FT   HELIX           168..172
FT                   /evidence="ECO:0007829|PDB:4GDA"
FT   HELIX           178..180
FT                   /evidence="ECO:0007829|PDB:2BC3"
SQ   SEQUENCE   183 AA;  18834 MW;  4652D8AE018468F5 CRC64;
     MRKIVVAAIA VSLTTVSITA SASADPSKDS KAQVSAAEAG ITGTWYNQLG STFIVTAGAD
     GALTGTYESA VGNAESRYVL TGRYDSAPAT DGSGTALGWT VAWKNNYRNA HSATTWSGQY
     VGGAEARINT QWLLTSGTTE ANAWKSTLVG HDTFTKVKPS AASIDAAKKA GVNNGNPLDA
     VQQ
//