Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methionine aminopeptidase

Gene

map

Organism
Methanothermus fervidus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val) (By similarity).By similarity

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.

Cofactori

Co2+By similarity, Zn2+By similarity, Mn2+By similarity, Fe2+By similarityNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60SubstrateBy similarity1
Metal bindingi80Divalent metal cation 1By similarity1
Metal bindingi91Divalent metal cation 1By similarity1
Metal bindingi91Divalent metal cation 2; catalyticBy similarity1
Metal bindingi164Divalent metal cation 2; catalytic; via tele nitrogenBy similarity1
Binding sitei172SubstrateBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Metal-binding

Protein family/group databases

MEROPSiM24.035.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase (EC:3.4.11.18)
Short name:
MAP
Short name:
MetAP
Alternative name(s):
Peptidase M
Gene namesi
Name:map
OrganismiMethanothermus fervidus
Taxonomic identifieri2180 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanothermaceaeMethanothermus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001489741 – ›188Methionine aminopeptidaseAdd BLAST›188

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi523846.Mfer_1063.

Structurei

3D structure databases

ProteinModelPortaliP22624.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG01001. Archaea.
COG0024. LUCA.

Family and domain databases

Gene3Di3.90.230.10. 1 hit.
InterProiIPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR018349. Pept_M24A_MAP2_BS.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P22624-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKFKKAGKI ASKVRKKAIK AVKGEMKILD LAEFIENEIE KMGAKPAFPC
60 70 80 90 100
NISVNEITAH YSPPCNDDRK ILPGDLVKID IGVHVDGFIG DTATTVLVEG
110 120 130 140 150
YEDLKNYNDE LAEKNKKMIE AAESALENAI NTIRDGVEIG KIGEVIENTI
160 170 180
NKFGFKPISN LTGHTIDRWV LHSGLSIPNV KGQNSHKL
Length:188
Mass (Da):20,713
Last modified:August 1, 1991 - v1
Checksum:i73057519CA497F62
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei1881

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26978 Genomic DNA. Translation: AAA72775.1.
PIRiPS0039.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26978 Genomic DNA. Translation: AAA72775.1.
PIRiPS0039.

3D structure databases

ProteinModelPortaliP22624.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi523846.Mfer_1063.

Protein family/group databases

MEROPSiM24.035.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiarCOG01001. Archaea.
COG0024. LUCA.

Family and domain databases

Gene3Di3.90.230.10. 1 hit.
InterProiIPR000994. Pept_M24.
IPR001714. Pept_M24_MAP.
IPR018349. Pept_M24A_MAP2_BS.
[Graphical view]
PfamiPF00557. Peptidase_M24. 1 hit.
[Graphical view]
PRINTSiPR00599. MAPEPTIDASE.
SUPFAMiSSF55920. SSF55920. 1 hit.
PROSITEiPS01202. MAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAP2_METFE
AccessioniPrimary (citable) accession number: P22624
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: August 1, 1991
Last modified: November 30, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.