Reviewed, UniProtKB/Swiss-Prot P22624 (AMPM_METFE)

Names and origin

Protein names	Recommended name:     Probable methionine aminopeptidase       Short name=MAP     EC=3.4.11.18 Alternative name(s):     Peptidase M
Organism	Methanothermus fervidus
Taxonomic identifier	2180 [NCBI]
Taxonomic lineage	Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanothermaceae › Methanothermus

Protein attributes

Sequence length	188 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›188	›188	Probable methionine aminopeptidase		PRO_0000148974
Sites
	Metal binding	80	1	Cobalt 1 By similarity
	Metal binding	91	1	Cobalt 1 By similarity
	Metal binding	91	1	Cobalt 2 By similarity
	Metal binding	164	1	Cobalt 2 By similarity
	Binding site	60	1	Substrate By similarity
	Binding site	172	1	Substrate By similarity
Experimental info
	Non-terminal residue	188	1

Sequences

	Sequence		Length	Mass (Da)
	P22624-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 73057519CA497F62	FASTA	188	20,713
	10 20 30 40 50 60 MEKFKKAGKI ASKVRKKAIK AVKGEMKILD LAEFIENEIE KMGAKPAFPC NISVNEITAH 70 80 90 100 110 120 YSPPCNDDRK ILPGDLVKID IGVHVDGFIG DTATTVLVEG YEDLKNYNDE LAEKNKKMIE 130 140 150 160 170 180 AAESALENAI NTIRDGVEIG KIGEVIENTI NKFGFKPISN LTGHTIDRWV LHSGLSIPNV KGQNSHKL

References

[1]	"Genes encoding 5S rRNA and tRNAs in the extremely thermophilic archaebacterium Methanothermus fervidus." Haas E.S., Daniels C.J., Reeve J.N. Gene 77:253-263(1989) [PubMed: 2473943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Novel protein families in archaean genomes." Ouzounis C., Kyrpides N., Sander C. Nucleic Acids Res. 23:565-570(1995) [PubMed: 7899076] [Abstract] Cited for: SIMILARITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M26978 Genomic DNA. Translation: AAA72775.1.
PIR	PS0039.
3D structure databases
HSSP	HSSP built from PDB template 1XGS based on UniProtKB P56218.
ModBase	Search...
Protein family/group databases
MEROPS	M24.002.
Enzyme and pathway databases
BRENDA	3.4.11.18. 7358.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. IPR018349. Pept_M24A_MAP2_BS. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHER	PTHR10804:SF13. Pept_M24A_MAP1. 1 hit. PTHR10804. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
PROSITE	PS01202. MAP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_METFE

Accession

Primary (citable) accession number: P22624

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)