P22621 (AMA1_PLAFF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Apical membrane antigen 1

Alternative name(s):
Merozoite surface antigen

Gene names

Name:	AMA-1
Synonyms:	PF83

Organism

Plasmodium falciparum (isolate FC27 / Papua New Guinea)

Taxonomic identifier

5837 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodium › Plasmodium (Laverania)

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in parasite invasion of erythrocytes.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the apicomplexan parasites AMA1 family.

Ontologies

Keywords
Cellular component	Membrane
Disease	Malaria
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Potential

Chain

25 – 622

598

Apical membrane antigen 1

PRO_0000024611

Regions

Topological domain

25 – 546

522

Extracellular Potential

Transmembrane

547 – 567

21

Helical; Potential

Topological domain

568 – 622

55

Cytoplasmic Potential

Amino acid modifications

Glycosylation

162

1

N-linked (GlcNAc...) Potential

Glycosylation

286

1

N-linked (GlcNAc...) Potential

Glycosylation

371

1

N-linked (GlcNAc...) Potential

Glycosylation

421

1

N-linked (GlcNAc...) Potential

Glycosylation

422

1

N-linked (GlcNAc...) Potential

Glycosylation

499

1

N-linked (GlcNAc...) Potential

Disulfide bond

149 ↔ 302

By similarity

Disulfide bond

217 ↔ 247

By similarity

Disulfide bond

263 ↔ 275

By similarity

Disulfide bond

320 ↔ 418

Ref.2

Disulfide bond

337 ↔ 409

Ref.2

Disulfide bond

443 ↔ 502

By similarity

Disulfide bond

490 ↔ 507

By similarity

Disulfide bond

492 ↔ 509

By similarity

Experimental info

Sequence conflict

308

1

Q → E in AAA29476. Ref.1

Sequence conflict

332

1

I → N in AAA29476. Ref.1

Sequence conflict

407

1

Q → H in AAA29476. Ref.1

Sequence conflict

439

1

H → N in AAA29476. Ref.1

Sequence conflict

496

1

I → M in AAA29476. Ref.1

Sequence conflict

503

1

N → R in AAA29476. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

622

Helix Strand Turn

Details...

Beta strand

316 – 319

4

Beta strand

327 – 329

3

Turn

331 – 333

3

Beta strand

334 – 336

3

Helix

345 – 347

3

Helix

357 – 359

3

Helix

361 – 365

5

Beta strand

368 – 370

3

Helix

371 – 374

4

Helix

375 – 377

3

Helix

386 – 388

3

Helix

393 – 395

3

Beta strand

400 – 403

4

Turn

404 – 407

4

Beta strand

408 – 412

5

Sequences

	Sequence		Length	Mass (Da)
	P22621 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 7D41335E249FA18F	FASTA	622	72,010
	10 20 30 40 50 60 MRKLYCVLLL SAFEFTYMIN FGRGQNYWEH PYQKSDVYHP INEHREHPKE YQYPLHQEHT 70 80 90 100 110 120 YQQEDSGEDE NTLQHAYPID HEGAEPAPQE QNLFSSIEIV ERSNYMGNPW TEYMAKYDIE 130 140 150 160 170 180 EVHGSGIRVD LGEDAEVAGT QYRLPSGKCP VFGKGIIIEN SNTTFLTPVA TGNQYLKDGG 190 200 210 220 230 240 FAFPPTEPLM SPMTLDEMRH FYKDNKYVKN LDELTLCSRH AGNMIPDNDK NSNYKYPAVY 250 260 270 280 290 300 DDKDKKCHIL YIAAQENNGP RYCNKDESKR NSMFCFRPAK DISFQNYTYL SKNVVDNWEK 310 320 330 340 350 360 VCPRKNLQNA KFGLWVDGNC EDIPHVNEFS AIDLFECNKL VFELSASDQP KQYEQHLTDY 370 380 390 400 410 420 EKIKEGFKNK NASMIKSAFL PTGAFKADRY KSHGKGYNWG NYNTETQKCE IFNVKPTCLI 430 440 450 460 470 480 NNSSYIATTA LSHPIEVEHN FPCSLYKNEI MKEIERESKR IKLNDNDDEG NKKIIAPRIF 490 500 510 520 530 540 ISDDKDSLKC PCDPEIVSNS TCNFFVCKCV ERRAEVTSNN EVVVKEEYKD EYADIPEHKP 550 560 570 580 590 600 TYDKMKIIIA SSAAVAVLAT ILMVYLYKRK GNAEKYDKMD EPQHYGKSNS RNDEMLDPEA 610 620 SFWGEEKRAS HTTPVLMEKP YY

References

[1]	"Integral membrane protein located in the apical complex of Plasmodium falciparum." Peterson M.G., Marshall V.M., Smythe J.A., Crewther P.E., Lew A., Silva A., Anders R.F., Kemp D.J. Mol. Cell. Biol. 9:3151-3154(1989) [PubMed: 2701947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"Structure and inter-domain interactions of domain II from the blood-stage malarial protein, apical membrane antigen 1." Feng Z.-P., Keizer D.W., Stevenson R.A., Yao S., Babon J.J., Murphy V.J., Anders R.F., Norton R.S. J. Mol. Biol. 350:641-656(2005) [PubMed: 15964019] [Abstract] Cited for: STRUCTURE BY NMR OF 309-436, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M27133 Genomic DNA. Translation: AAA29475.1.
M27957 mRNA. Translation: AAA29476.1.

PIR

A32499.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YXE	NMR	-	A	309-436	[»]
2Q8A	X-ray	2.40	A	104-438	[»]
2Q8B	X-ray	2.30	A	104-438	[»]

ProteinModelPortal

P22621.

SMR

P22621. Positions 96-545.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR003298. Apmem_Ag1.
IPR024056. Apmem_Ag1_domain.
[Graphical view]

Gene3D

G3DSA:4.10.1010.10. G3DSA:4.10.1010.10. 1 hit.

Pfam

PF02430. AMA-1. 1 hit.
[Graphical view]

PRINTS

PR01361. MEROZOITESA.

SMART

SM00815. AMA-1. 1 hit.
[Graphical view]

SUPFAM

SSF82910. SSF82910. 1 hit.

ProtoNet

Search...

Entry information

Entry name

AMA1_PLAFF

Accession

Primary (citable) accession number: P22621

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	July 27, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)