P22600 (HEMH_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferrochelatase, mitochondrial
EC=4.99.1.1

Alternative name(s):
Heme synthase
Protoheme ferro-lyase

Gene names

Name:

FECH

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX.
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+.
Cofactor	Binds 1 2Fe-2S cluster.
Enzyme regulation	Inhibited by nitric oxide (NO). The 2Fe-2S cluster could act as a NO sensor By similarity.
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.
Subunit structure	Monomer By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW ferrochelatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 47	47	Mitochondrion Potential
	Chain	48 – 416	369	Ferrochelatase, mitochondrial		PRO_0000008872
Sites
	Active site	223	1	By similarity
	Active site	376	1	By similarity
	Metal binding	189	1	Iron-sulfur (2Fe-2S)
	Metal binding	396	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	399	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	404	1	Iron-sulfur (2Fe-2S) By similarity
Experimental info
	Sequence conflict	282	1	D → E AA sequence Ref.2
	Sequence conflict	287	1	S → P AA sequence Ref.2
	Sequence conflict	287	1	S → P AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P22600 [UniParc]. Last modified February 1, 1996. Version 3. Checksum: ECE1D43F9DC4B78E	FASTA	416	46,935
	10 20 30 40 50 60 MAAALRSAGV LLRDRLLYGG SRACQPRRCQ SGAATAAAAT ETAQRARSPK PQAQPGNRKP 70 80 90 100 110 120 RTGILMLNMG GPETVEEVQD FLQRLFLDQD LMTLPVQDKL GPFIAKRRTP KIQEQYRRIG 130 140 150 160 170 180 GGSPIKMWTS KQGEGMVKLL DELSPHTAPH KYYIGFRYVH PLTEEAIEEM ERDGLERAVA 190 200 210 220 230 240 FTQYPQYSCS TTGSSLNAIY RYYNEVGRKP TMKWSTIDRW PTHPLLIQCF ADHILKELDH 250 260 270 280 290 300 FPPEKRREVV ILFSAHSLPM SVVNRGDPYP QEVGATVQRV MDKLGYSNPY RLVWQSKVGP 310 320 330 340 350 360 MPWLGPQTDE AIKGLCKRGR KNILLVPIAF TSDHIETLYE LDIEYSQVLA SECGLENIRR 370 380 390 400 410 AESLNGNPLF SKALADLVHS HLQSKERCST QLTLSCPLCV NPTCRETKSF FTSQQL

References

[1]	"The coding sequence of the bovine ferrochelatase gene." Shibuya H., Nonneman D., Tamassia M., Allphin O.L., Johnson G.S. Biochim. Biophys. Acta 1231:117-120(1995) [PubMed: 7640290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of murine ferrochelatase." Brenner D.A., Frasier F. Proc. Natl. Acad. Sci. U.S.A. 88:849-853(1991) [PubMed: 1704134] [Abstract] Cited for: PROTEIN SEQUENCE OF 281-311. Tissue: Liver.
[3]	"Molecular cloning, sequencing, and expression of mouse ferrochelatase." Taketani S., Nakahashi Y., Osumi T., Tokunaga R. J. Biol. Chem. 265:19377-19380(1990) [PubMed: 2246229] [Abstract] Cited for: PROTEIN SEQUENCE OF 152-157; 266-275; 284-291 AND 373-381.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L34173 mRNA. Translation: AAA79169.1.
IPI	IPI00701579.
PIR	I45890.
RefSeq	NP_776479.1. NM_174054.2.
UniGene	Bt.88608.
3D structure databases
ProteinModelPortal	P22600.
SMR	P22600. Positions 58-416.
ModBase	Search...
Protein-protein interaction databases
STRING	P22600.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000008384; ENSBTAP00000008384; ENSBTAG00000006393.
GeneID	281158.
KEGG	bta:281158.
Organism-specific databases
CTD	2235.
Phylogenomic databases
eggNOG	maNOG04866.
GeneTree	ENSGT00390000016258.
HOVERGEN	HBG051898.
InParanoid	P22600.
OMA	LQKPLAW.
OrthoDB	EOG4GXFN0.
PhylomeDB	P22600.
Family and domain databases
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
KO	K01772.
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. HemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BOVIN

Accession

Primary (citable) accession number: P22600

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	February 1, 1996
Last modified:	November 16, 2011
This is version 97 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program