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Protein

Alpha-1-antitrypsin 1-2

Gene

Serpina1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin.2 Publications

Miscellaneous

Murine alpha-1-antitrypsin is represented by a cluster of up to 6 individual Serpina1-related genes. The precise complement of Serpina1-related genes present varies according to the strain of the animal.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei377 – 378Reactive bondBy similarity2

GO - Molecular functioni

  • glycoprotein binding Source: MGI
  • identical protein binding Source: MGI
  • protease binding Source: MGI
  • serine-type endopeptidase inhibitor activity Source: MGI

GO - Biological processi

  • acute-phase response Source: UniProtKB-KW
  • in utero embryonic development Source: MGI
  • protein N-linked glycosylation Source: MGI
  • response to cytokine Source: MGI
  • response to peptide hormone Source: MGI

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor
Biological processAcute phase

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
R-MMU-5694530. Cargo concentration in the ER.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-8957275. Post-translational protein phosphorylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1-antitrypsin 1-2
Short name:
AAT
Alternative name(s):
Alpha-1 protease inhibitor 2
Alpha-1-antiproteinase
Serine protease inhibitor 1-2
Serine protease inhibitor A1b
Short name:
Serpin A1b
Gene namesi
Name:Serpina1b
Synonyms:Aat2, Dom2, Spi1-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:891970. Serpina1b.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24By similarityAdd BLAST24
ChainiPRO_000003238925 – 413Alpha-1-antitrypsin 1-2Add BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi64N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi101N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi265N-linked (GlcNAc...) asparagine1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP22599.
PaxDbiP22599.
PeptideAtlasiP22599.
PRIDEiP22599.

PTM databases

iPTMnetiP22599.
PhosphoSitePlusiP22599.

Expressioni

Gene expression databases

BgeeiENSMUSG00000071178.
ExpressionAtlasiP22599. baseline and differential.
GenevisibleiP22599. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203428. 1 interactor.
IntActiP22599. 2 interactors.
MINTiMINT-1855477.
STRINGi10090.ENSMUSP00000093101.

Structurei

3D structure databases

ProteinModelPortaliP22599.
SMRiP22599.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni368 – 387RCLAdd BLAST20

Domaini

The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable (By similarity). Variability within the reactive center loop (RCL) sequences of Serpina1-related genes may determine target protease specificity.By similarity

Sequence similaritiesi

Belongs to the serpin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00760000118839.
HOGENOMiHOG000238521.
HOVERGENiHBG005957.
InParanoidiP22599.
KOiK03984.
OMAiARRPMEF.
OrthoDBiEOG091G0ION.
PhylomeDBiP22599.
TreeFamiTF343201.

Family and domain databases

InterProiView protein in InterPro
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiView protein in Pfam
PF00079. Serpin. 1 hit.
SMARTiView protein in SMART
SM00093. SERPIN. 1 hit.
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiView protein in PROSITE
PS00284. SERPIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P22599-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPSISWGLL LLAGLCCMVP SFLAEDVQET DTSQKDQSPA SHEIATNLGD
60 70 80 90 100
FAISLYRELV HQSNTSNIFF SPVSIATAFA MLSLGSKGDT HTQILEGLQF
110 120 130 140 150
NLTQTSEADI HKSFQHLLQT LNRPDSELQL STGNGLFVNN DLKLVEKFLE
160 170 180 190 200
EAKNHYQAEV FSVNFAESEE AKKVINDFVE KGTQGKIVEA VKELDQDTVF
210 220 230 240 250
ALANYILFKG KWKKPFDPEN TEEAEFHVDK STTVKVPMMM LSGMLDVHHC
260 270 280 290 300
SILSSWVLLM DYAGNASAVF LLPEDGKMQH LEQTLNKELI SKILLNRRRR
310 320 330 340 350
LVQIHIPRLS ISGDYNLKTL MSPLGITRIF NNGADLSGIT EENAPLKLSK
360 370 380 390 400
AVHKAVLTID ETGTEAAAAT VFEAVPMSMP PILRFDHPFL FIIFEEHTQS
410
PIFVGKVVDP THK
Length:413
Mass (Da):45,975
Last modified:June 21, 2005 - v2
Checksum:i92BD12B6D4768D7B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18M → L (PubMed:2303252).Curated1
Sequence conflicti18M → L (PubMed:12408969).Curated1
Sequence conflicti18M → L in AAH22109 (PubMed:15489334).Curated1
Sequence conflicti18M → L in AAH25445 (PubMed:15489334).Curated1
Sequence conflicti18M → L in AAH49255 (PubMed:15489334).Curated1
Sequence conflicti203A → G in AAA37132 (PubMed:2303252).Curated1
Sequence conflicti240M → T (PubMed:2303252).Curated1
Sequence conflicti240M → T (PubMed:12408969).Curated1
Sequence conflicti240M → T in AAH22109 (PubMed:15489334).Curated1
Sequence conflicti240M → T in AAH25445 (PubMed:15489334).Curated1
Sequence conflicti240M → T in AAH49255 (PubMed:15489334).Curated1
Sequence conflicti252I → T (PubMed:2303252).Curated1
Sequence conflicti252I → T (PubMed:12408969).Curated1
Sequence conflicti252I → T in AAH22109 (PubMed:15489334).Curated1
Sequence conflicti252I → T in AAH25445 (PubMed:15489334).Curated1
Sequence conflicti252I → T in AAH49255 (PubMed:15489334).Curated1
Sequence conflicti263A → V in AAM47489 (PubMed:12408969).Curated1
Sequence conflicti263A → V in AAH22109 (PubMed:15489334).Curated1
Sequence conflicti263A → V in AAH25445 (PubMed:15489334).Curated1
Sequence conflicti263A → V in AAH49255 (PubMed:15489334).Curated1
Sequence conflicti286N → S in AAM47489 (PubMed:12408969).Curated1
Sequence conflicti286N → S in AAH22109 (PubMed:15489334).Curated1
Sequence conflicti286N → S in AAH25445 (PubMed:15489334).Curated1
Sequence conflicti286N → S in AAH49255 (PubMed:15489334).Curated1
Sequence conflicti298R → H in AAM47489 (PubMed:12408969).Curated1
Sequence conflicti298R → H in AAH22109 (PubMed:15489334).Curated1
Sequence conflicti298R → H in AAH25445 (PubMed:15489334).Curated1
Sequence conflicti298R → H in AAH49255 (PubMed:15489334).Curated1
Sequence conflicti314D → E in AAA37132 (PubMed:2303252).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25529 mRNA. Translation: AAA37132.1.
AF481949 Genomic DNA. Translation: AAM47489.1.
BC022109 mRNA. Translation: AAH22109.1.
BC025445 mRNA. Translation: AAH25445.1.
BC049255 mRNA. Translation: AAH49255.2.
M75716 mRNA. Translation: AAC28865.1.
CCDSiCCDS26138.1.
PIRiI49452.
I49471.
RefSeqiNP_033270.3. NM_009244.4.
UniGeneiMm.439692.
Mm.439693.
Mm.439695.

Genome annotation databases

EnsembliENSMUST00000095450; ENSMUSP00000093101; ENSMUSG00000071178.
ENSMUST00000164454; ENSMUSP00000127266; ENSMUSG00000071178.
ENSMUST00000186166; ENSMUSP00000139941; ENSMUSG00000071178.
GeneIDi20701.
KEGGimmu:20701.
UCSCiuc007owe.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25529 mRNA. Translation: AAA37132.1.
AF481949 Genomic DNA. Translation: AAM47489.1.
BC022109 mRNA. Translation: AAH22109.1.
BC025445 mRNA. Translation: AAH25445.1.
BC049255 mRNA. Translation: AAH49255.2.
M75716 mRNA. Translation: AAC28865.1.
CCDSiCCDS26138.1.
PIRiI49452.
I49471.
RefSeqiNP_033270.3. NM_009244.4.
UniGeneiMm.439692.
Mm.439693.
Mm.439695.

3D structure databases

ProteinModelPortaliP22599.
SMRiP22599.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203428. 1 interactor.
IntActiP22599. 2 interactors.
MINTiMINT-1855477.
STRINGi10090.ENSMUSP00000093101.

PTM databases

iPTMnetiP22599.
PhosphoSitePlusiP22599.

Proteomic databases

MaxQBiP22599.
PaxDbiP22599.
PeptideAtlasiP22599.
PRIDEiP22599.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095450; ENSMUSP00000093101; ENSMUSG00000071178.
ENSMUST00000164454; ENSMUSP00000127266; ENSMUSG00000071178.
ENSMUST00000186166; ENSMUSP00000139941; ENSMUSG00000071178.
GeneIDi20701.
KEGGimmu:20701.
UCSCiuc007owe.1. mouse.

Organism-specific databases

CTDi20701.
MGIiMGI:891970. Serpina1b.

Phylogenomic databases

eggNOGiKOG2392. Eukaryota.
COG4826. LUCA.
GeneTreeiENSGT00760000118839.
HOGENOMiHOG000238521.
HOVERGENiHBG005957.
InParanoidiP22599.
KOiK03984.
OMAiARRPMEF.
OrthoDBiEOG091G0ION.
PhylomeDBiP22599.
TreeFamiTF343201.

Enzyme and pathway databases

ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
R-MMU-5694530. Cargo concentration in the ER.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-8957275. Post-translational protein phosphorylation.

Miscellaneous databases

PROiPR:P22599.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000071178.
ExpressionAtlasiP22599. baseline and differential.
GenevisibleiP22599. MM.

Family and domain databases

InterProiView protein in InterPro
IPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
PANTHERiPTHR11461. PTHR11461. 1 hit.
PfamiView protein in Pfam
PF00079. Serpin. 1 hit.
SMARTiView protein in SMART
SM00093. SERPIN. 1 hit.
SUPFAMiSSF56574. SSF56574. 1 hit.
PROSITEiView protein in PROSITE
PS00284. SERPIN. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiA1AT2_MOUSE
AccessioniPrimary (citable) accession number: P22599
Secondary accession number(s): Q61283, Q80ZH5, Q8VC20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1991
Last sequence update: June 21, 2005
Last modified: June 7, 2017
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.