Reviewed, UniProtKB/Swiss-Prot P22580 (AMDY_YEAST)

Names and origin

Protein names

Recommended name:
    Probable amidase
    EC=3.5.1.4

Gene names

Name:	AMD2
Synonyms:	AMDY, AMDY1
Ordered Locus Names:	YDR242W
ORF Names:	YD8419.09

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A monocarboxylic acid amide + H2O = a monocarboxylate + NH3.
Sequence similarities	Belongs to the amidase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	amidase activity Inferred from electronic annotation. Source: EC carbon-nitrogen ligase activity, with glutamine as amido-N-donor Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-2044220,EBI-24597

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Probable amidase		PRO_0000105130
Sites
	Active site	132	1	Charge relay system By similarity
	Active site	209	1	Charge relay system By similarity
	Active site	233	1	Acyl-ester intermediate By similarity
Experimental info
	Sequence conflict	525	1	G → S in CAA39514. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P22580-1 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: E7CB049845C1FCDC	FASTA	549	61,410
	10 20 30 40 50 60 MTVHSTWKEK VQLKKDQLNS KIKDEWKLNS TTITRLKNDK KNLIKNIDDL CSSSENQITH 70 80 90 100 110 120 STIMALRQAL EAKELSCHEI TAAFCHRAAL IHQVVNCLSE IMFSEALRLA DYYDSNRPAI 130 140 150 160 170 180 LPPLYGIPIS LKDQCNVEGV DTSLGYLCRT FKPKTKNEES LIVSFLRDLG AIIFVKTTVP 190 200 210 220 230 240 SSMMATDTQS NTFGYTYNSI NLSFSSGGSS GGEGSLIGAH GSLLGLGTDI GGSIRIPSSY 250 260 270 280 290 300 QGLFGLKPTF GRVPYLRVDN SFEGRETIPS VIGPLARDLS DLRYFMSCVI NICQPWVQDV 310 320 330 340 350 360 KCIPYHFDSS TSKLHDNYVV GIWYGDGVID PPPSDIRALK TCEDLVNKTK GMKAVKWEPS 370 380 390 400 410 420 SELSRELFDL ANEADVADSG NEIKNEFEIS GEPLLDILKP MVLENGRPPY TVNEWWDLTK 430 440 450 460 470 480 RVYNAQQLMR DYYLSFPESE RPDVIISPTT LMPFRPGDML KTTLRYILLF NVLNFPSLSI 490 500 510 520 530 540 PVGSVDCQID GLMDTTSALN PEDKMIKTYW NDLIQSGEID GFPIGLQVVS PTFNDNEVCK FASWLFSKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a putative amidase gene in yeast Saccharomyces cerevisiae." Chang T.-H., Abelson J. Nucleic Acids Res. 18:7180-7180(1990) [PubMed: 2263500] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X56043 Genomic DNA. Translation: CAA39514.1. Z49701 Genomic DNA. Translation: CAA89728.1. AY723784 Genomic DNA. Translation: AAU09701.1.
PIR	S54538.
RefSeq	NP_010528.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P22580. 1 interaction.
STRING	P22580.
Genome annotation databases
Ensembl	YDR242W; YDR242W; YDR242W; Saccharomyces cerevisiae. [Genome view]
GeneID	851829.
KEGG	sce:YDR242W.
NMPDR	fig|4932.3.peg.1285.
Organism-specific databases
CYGD	YDR242w.
SGD	S000002650. AMD2.
Phylogenomic databases
HOGENOM	HBG327754.
OMA	STEGQEH.
OrthoDB	EOG9SXPVN.
Enzyme and pathway databases
BRENDA	3.5.1.4. 250.
Gene expression databases
ArrayExpress	P22580.
Genevestigator	P22580.
GermOnline	YDR242W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000120. Amidase. IPR020556. Amidase_CS. IPR015830. Amidase_fun. [Graphical view]
Gene3D	G3DSA:3.90.1300.10. Amidase_sig_enz. 1 hit.
PANTHER	PTHR11895. Amidase. 1 hit.
Pfam	PF01425. Amidase. 1 hit. [Graphical view]
PIRSF	PIRSF001221. Amidase_fungi. 1 hit.
PROSITE	PS00571. AMIDASES. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	969712.

Entry information

Entry name

AMDY_YEAST

Accession

Primary (citable) accession number: P22580

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	February 1, 1996
Last modified:	December 15, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)