P22580 (AMDY_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable amidase
EC=3.5.1.4

Gene names

Name:	AMD2
Synonyms:	AMDY, AMDY1
Ordered Locus Names:	YDR242W
ORF Names:	YD8419.09

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A monocarboxylic acid amide + H2O = a monocarboxylate + NH3.
Sequence similarities	Belongs to the amidase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	amidase activity Inferred from electronic annotation. Source: EC carbon-nitrogen ligase activity, with glutamine as amido-N-donor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Probable amidase		PRO_0000105130
Sites
	Active site	132	1	Charge relay system By similarity
	Active site	209	1	Charge relay system By similarity
	Active site	233	1	Acyl-ester intermediate By similarity
Experimental info
	Sequence conflict	525	1	G → S in CAA39514. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P22580 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: E7CB049845C1FCDC	FASTA	549	61,410
	10 20 30 40 50 60 MTVHSTWKEK VQLKKDQLNS KIKDEWKLNS TTITRLKNDK KNLIKNIDDL CSSSENQITH 70 80 90 100 110 120 STIMALRQAL EAKELSCHEI TAAFCHRAAL IHQVVNCLSE IMFSEALRLA DYYDSNRPAI 130 140 150 160 170 180 LPPLYGIPIS LKDQCNVEGV DTSLGYLCRT FKPKTKNEES LIVSFLRDLG AIIFVKTTVP 190 200 210 220 230 240 SSMMATDTQS NTFGYTYNSI NLSFSSGGSS GGEGSLIGAH GSLLGLGTDI GGSIRIPSSY 250 260 270 280 290 300 QGLFGLKPTF GRVPYLRVDN SFEGRETIPS VIGPLARDLS DLRYFMSCVI NICQPWVQDV 310 320 330 340 350 360 KCIPYHFDSS TSKLHDNYVV GIWYGDGVID PPPSDIRALK TCEDLVNKTK GMKAVKWEPS 370 380 390 400 410 420 SELSRELFDL ANEADVADSG NEIKNEFEIS GEPLLDILKP MVLENGRPPY TVNEWWDLTK 430 440 450 460 470 480 RVYNAQQLMR DYYLSFPESE RPDVIISPTT LMPFRPGDML KTTLRYILLF NVLNFPSLSI 490 500 510 520 530 540 PVGSVDCQID GLMDTTSALN PEDKMIKTYW NDLIQSGEID GFPIGLQVVS PTFNDNEVCK FASWLFSKI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a putative amidase gene in yeast Saccharomyces cerevisiae." Chang T.-H., Abelson J. Nucleic Acids Res. 18:7180-7180(1990) [PubMed: 2263500] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed: 9169867] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56043 Genomic DNA. Translation: CAA39514.1. Z49701 Genomic DNA. Translation: CAA89728.1. AY723784 Genomic DNA. Translation: AAU09701.1. BK006938 Genomic DNA. Translation: DAA12082.1.
PIR	S54538.
RefSeq	NP_010528.1. NM_001180550.1.
3D structure databases
ProteinModelPortal	P22580.
ModBase	Search...
Protein-protein interaction databases
IntAct	P22580. 1 interaction.
STRING	P22580.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YDR242W; YDR242W; YDR242W.
GeneID	851829.
KEGG	sce:YDR242W.
NMPDR	fig|4932.3.peg.1285.
Organism-specific databases
CYGD	YDR242w.
SGD	S000002650. AMD2.
Phylogenomic databases
eggNOG	fuNOG06476.
GeneTree	EFGT00050000001234.
HOGENOM	HBG327754.
OMA	SENQITH.
OrthoDB	EOG4M68RW.
Gene expression databases
ArrayExpress	P22580.
Genevestigator	P22580.
GermOnline	YDR242W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000120. Amidase. IPR020556. Amidase_CS. IPR023631. Amidase_dom. IPR015830. Amidase_fun. [Graphical view]
Gene3D	G3DSA:3.90.1300.10. Amidase_sig_enz. 1 hit.
KO	K01426.
PANTHER	PTHR11895. Amidase. 1 hit. PTHR11895:SF4. PTHR11895:SF4. 1 hit.
Pfam	PF01425. Amidase. 1 hit. [Graphical view]
PIRSF	PIRSF001221. Amidase_fungi. 1 hit.
SUPFAM	SSF75304. Amidase_sig_enz. 1 hit.
PROSITE	PS00571. AMIDASES. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	969712.

Entry information

Entry name

AMDY_YEAST

Accession

Primary (citable) accession number: P22580
Secondary accession number(s): D6VSM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program