Reviewed, UniProtKB/Swiss-Prot P22573 (DYR_SHV2C)

Names and origin

Protein names

Recommended name:
    Viral dihydrofolate reductase
      Short name=vDHFR
    EC=1.5.1.3

Gene names

Name:	DHFR
Synonyms:	2

Organism

Saimiriine herpesvirus 2 (strain 488) (SaHV-2) (Herpesvirus saimiri)

Taxonomic identifier

10384 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Gammaherpesvirinae › Rhadinovirus

Virus host

Saimiri sciureus (Common squirrel monkey) [TaxID: 9521]

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.
Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	Viral dihydrofolate reductase		PRO_0000186381
Regions
	Domain	4 – 184	181	DHFR
	Nucleotide binding	16 – 22	7	NADP By similarity
	Nucleotide binding	54 – 56	3	NADP By similarity
	Nucleotide binding	76 – 78	3	NADP By similarity
	Nucleotide binding	116 – 123	8	NADP By similarity
	Region	31 – 36	6	Substrate binding By similarity
Sites
	Binding site	10	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	70	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P22573-1 [UniParc]. Last modified August 1, 1991. Version 1. Checksum: 932BBB2EA200033C	FASTA	213	24,577
	10 20 30 40 50 60 MVLLLNCIVA VDQNMGIGKK GHLPWPLLIN DFKYFQRMTT SSVKNKQNLV IMGKNTWFSI 70 80 90 100 110 120 PEKNRPLKDR INLVLSKKLK EIPHGAHFLA RSLNDALKLI EQPELVNKVD RVWIIGGSSV 130 140 150 160 170 180 YKDAMNYSSH LKLFVTRIMQ SFETDTFFPE IDLKNYKLLI EYPGVPSNTQ EEKGIKYKFE 190 200 210 VYEKIVNTFL FKSHAGLTCS VKPKEASYDF ELS

References

[1]	"The divergence between two oncogenic Herpesvirus saimiri strains in a genomic region related to the transforming phenotype." Biesinger B., Trimble J.J., Desrosiers R.C., Fleckenstein B. Virology 176:505-514(1990) [PubMed: 2161148] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M55264 Genomic DNA. Translation: AAA72932.1.
3D structure databases
SMR	P22573. Positions 5-184.
ModBase	Search...
Family and domain databases
InterPro	IPR012259. DHFR. IPR017925. Dihydrofolate_reductase_CS. IPR001796. Dihydrofolate_reductase_dom. [Graphical view]
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR_SHV2C

Accession

Primary (citable) accession number: P22573

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	August 1, 1991
Last modified:	February 9, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)