Reviewed, UniProtKB/Swiss-Prot P22567 (ARGA_PSEAE)

Names and origin

Protein names

Recommended name:
    Amino-acid acetyltransferase
    EC=2.3.1.1
Alternative name(s):
    N-acetylglutamate synthase
      Short name=AGS
      Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	PA5204

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.
Sequence caution	The sequence AAA73977.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino-acid N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Amino-acid acetyltransferase HAMAP MF_01105		PRO_0000186798
Regions
	Domain	286 – 425	140	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	P22567-1 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 725FAD778FD44A4E	FASTA	432	47,859
	10 20 30 40 50 60 MPDYVNWLRH ASPYINSHRD RTFVVMLPGE GVEHPNFGNI VHDLVLLHSL GARLVLVHGS 70 80 90 100 110 120 RPQIEARLAA RGLAPRYHRD LRVTDAPTLE CVIDAVGSLR IAIEARLSMD MAASPMQGAR 130 140 150 160 170 180 LRVAGGNLVT ARPIGVVEGV DYHHTGEVRR IDRKGIGRLL DERSIVLLSP LGYSPTGEIF 190 200 210 220 230 240 NLACEDVAMR AAIDLEAEKL ILYGAEQGLL DASGKLVREL RPQQVPAHLQ RLGNSYQAEL 250 260 270 280 290 300 LDAAAQACRA GVKRSHIVSY TEDGALLSEL FTRTGNGTLV AQEQFEQLRE AGIEDVGGLI 310 320 330 340 350 360 ELIRPLEEQG ILVRRSREVL EREIEQFSIV EREGLIIACA ALYPIADSEA GELACLAVNP 370 380 390 400 410 420 EYRHGGRGDE LLERIEERAR GLGLKTLFVL TTRTAHWFRE RGFQPSSVER LPAARASLYN 430 FQRNSQVFEK SL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Dharmsthiti S., Krishnapillai V.V. Submitted (FEB-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PAO.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M38358 Genomic DNA. Translation: AAA73977.1. Frameshift. AE004091 Genomic DNA. Translation: AAG08589.1.
PIR	G82995. S27600.
RefSeq	NP_253891.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	879475.
GenomeReviews	Gene locus PA5204 in contig AE004091_GR.
KEGG	pae:PA5204.
Organism-specific databases
PseudoCAP	PA5204.
CMR	Search...
Phylogenomic databases
HOGENOM	P22567.
OMA	DLIRPLE.
Enzyme and pathway databases
BioCyc	PAER208964:PA5204-MON.
BRENDA	2.3.1.1. 354.
Family and domain databases
HAMAP	MF_01105. [Tree]
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR000182. GCN5-rel_AcTrfase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_PSEAE

Accession

Primary (citable) accession number: P22567

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1991
Last sequence update:	December 8, 2000
Last modified:	November 3, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)